Article
Multidisciplinary Sciences
Qi Yan, Wenqing Zhang, Mingqun Lin, Omid Teymournejad, Khemraj Budachetri, Jeffrey Lakritz, Yasuko Rikihisa
Summary: Etf-3 is a bacterial protein that induces ferritinophagy to facilitate pathogen iron capture, allowing the bacterium to acquire iron for survival and proliferation in the host cell.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Microbiology
Jiaqi Yan, Qi'an Liang, Zhouyi Chai, Nan Duan, Xiaoxiao Li, Yajing Liu, Nan Yang, Meifang Li, Yongxin Jin, Fang Bai, Weihui Wu, Zhihui Cheng
Summary: Ehrlichia chaffeensis utilizes two enzymes, GshA and GshB, to synthesize glutathione and a global transcriptional regulator, CtrA, to resist oxidative stress. These findings provide insights into the adaptation and pathogenesis of E. chaffeensis in hosts.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Microbiology
Madison R. Rogan, LaNisha L. Patterson, Caitlan D. Byerly, Tian Luo, Slobodan Paessler, Veljko Veljkovic, Bethany Quade, Jere W. McBride
Summary: This study reveals that the protein TRP120 of Ehrlichia chaffeensis interacts directly with components of the Wnt receptor complex, influencing pathway activity and promoting infection. This finding highlights a unique mechanism of bacterial mimicry of Wnt pathway ligands and provides insights into potential antimicrobial therapeutic targets.
Article
Multidisciplinary Sciences
Wenqing Zhang, Mingqun Lin, Qi Yan, Khemraj Budachetri, Libo Hou, Ashweta Sahni, Hongyan Liu, Nien-Ching Han, Jeffrey Lakritz, Dehua Pei, Yasuko Rikihisa
Summary: The study developed a specific nanobody, NbD7, that inhibits T4SS effector functions and Ehrlichia chaffeensis infection. NbD7 was successfully delivered into host cells by conjugating to cell-permeable peptide 12 (CPP12-NbD7), effectively blocking infection and overcoming current barriers in research and therapy development.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Immunology
Deborah C. Jaworski, Ying Wang, Arathy Nair, Huitao Liu, Roman R. Ganta
Summary: This study identified 55 mutations in E. chaffeensis, which demonstrated the critical nature of these genes for its growth in a canine infection model. Four mutations resulted in replication deficiency in the tick vector, similar to the vertebrate host. Additionally, three mutations caused growth defects only in the tick vector. The findings suggest that genes essential for growth in the canine host may not be essential for growth in the tick vector.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Samira Sabzi, Shahla Shahbazi, Narjes Noori Goodarzi, Fatemeh Haririzadeh Jouriani, Mehri Habibi, Negin Bolourchi, Amir Mirzaie, Farzad Badmasti
Summary: This study aims to develop an effective drug and vaccine against Ehrlichia chaffeensis. Through protein and epitope analysis, potential drug targets and vaccine candidates were identified, and a multi-epitope vaccine was designed. These findings may have important implications for the treatment and prevention of Ehrlichia chaffeensis, but further experimental research is needed for validation.
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
(2023)
Review
Immunology
Yasuko Rikihisa
Summary: Ehrlichia chaffeensis is an intracellular bacterium that causes human monocytic ehrlichiosis. It enters human cells through binding to a specific receptor and manipulates host-cell functions for replication. Future research on the molecular mechanisms of this bacterium and its interaction with host-cell components may lead to advancements in the understanding and treatment of ehrlichiosis.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Immunology
Nan Duan, Xiaohui Ma, Heting Cui, Zhexuan Wang, Zhouyi Chai, Jiaqi Yan, Xiaoxiao Li, Yingxing Feng, Yu Cao, Yongxin Jin, Fang Bai, Weihui Wu, Yasuko Rikihisa, Zhihui Cheng
Summary: The study demonstrates that transcriptional regulators EcxR and Tr1 regulate the differential expression of omp-1B and p28 in E. chaffeensis. Tr1 shows a higher affinity towards the p28 promoter than the omp-1B promoter and can activate or repress gene expression based on temperature conditions. This insight into novel gene regulation mechanisms may contribute to the development of new therapeutics for HME.
EMERGING MICROBES & INFECTIONS
(2021)
Article
Chemistry, Medicinal
Jiayao Tian, Shang Chen, Fei Liu, Qian Zhu, Jianzhong Shen, Wenhan Lin, Kui Zhu
Summary: The marine-derived compound equisetin efficiently eliminates intracellular Staphylococcus aureus by potentiating host autophagy and inducing ROS generation, showing remarkable anti-infection activity in a mouse model.
Article
Multidisciplinary Sciences
Alex B. Benedict, Prithwi Ghosh, Samuel M. Scott, Joel S. Griffitts
Summary: The symbiosis between Medicago truncatula and Sinorhizobium meliloti involves chemical signaling that initiates rhizobial infection in root nodule tissue, leading to nitrogen fixation. Plant-derived NCR peptides play a crucial role in inducing physiological changes in the intracellular bacteria. The discovery of the symbiosis-associated peptidase gene sapA suggests that specific bacterial factors can influence the symbiotic outcome, with its overexpression resulting in a significant decrease in plant fitness.
SCIENTIFIC REPORTS
(2021)
Article
Microbiology
Mirelys Hernandez-Morfa, Nicolas M. Reinoso-Vizcaino, Nadia B. Olivero, Victoria E. Zappia, Paulo R. Cortes, Andrea Jaime, Jose Echenique
Summary: This study reveals the fluoroquinolone persistence mechanism in Streptococcus pneumoniae, which is induced by oxidative stress and allows the bacteria to survive in host cells. The findings suggest that persistence may influence antibiotic therapy and contribute to the evolution of fluoroquinolone resistance.
MICROBIOLOGY SPECTRUM
(2022)
Review
Biochemistry & Molecular Biology
Malte Kellermann, Felix Scharte, Michael Hensel
Summary: Pathogenic intracellular microorganisms manipulate host cells to create niches for survival and replication. This review focuses on the manipulation of organelle compartments by pathogens and highlights the specific interactions of different pathogens with host cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Kendi Okuda, Miriam Maria Silva Costa Franco, Ari Yasunaga, Ricardo Gazzinelli, Michel Rabinovitch, Sara Cherry, Neal Silverman
Summary: Leishmania parasites utilize virulence mechanisms to invade and replicate in macrophages, inhibiting host defenses and altering gene expression to enhance their survival and replication.
Article
Immunology
Yuntao Zhang, Li Chen, Chandramouli Kondethimmanahalli, Huitao Liu, Roman R. Ganta
Summary: The study explores the use of an axenic medium for the growth of two important rickettsiae pathogens in host cell-free phagosomes. It demonstrates the synthesis of bacterial DNA and protein in host cell-free phagosomes for the first time, providing new insights into the replication and growth of these pathogens. The findings highlight the importance of host cell support-free axenic growth in advancing research on tick-borne diseases affecting human and animal health.
INFECTION AND IMMUNITY
(2021)
Article
Immunology
Shanshan Liang, Hui Dong, Shunhai Zhu, Qiping Zhao, Bing Huang, Yu Yu, Qingjie Wang, Haixia Wang, Shuilan Yu, Hongyu Han
Summary: A study investigated the interaction between a potential interactor, EteIF-5A, and EtCDPK4 in Eimeria tenella, as well as the expression patterns of EteIF-5A in different developmental stages. Results showed that anti-rEteIF-5A polyclonal antibodies effectively inhibited cell invasion by sporozoites, indicating its potential as a therapeutic target.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Immunology
Tian Luo, Paige S. Dunphy, Taslima T. Lina, Jere W. McBride
INFECTION AND IMMUNITY
(2016)
Article
Microbiology
Taslima T. Lina, Paige S. Dunphy, Tian Luo, Jere W. McBride
Review
Immunology
Taslima T. Lina, Tierra Farris, Tian Luo, Shubhajit Mitra, Bing Zhu, Jere W. McBride
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2016)
Article
Immunology
Tian Luo, Paige S. Dunphy, Jere W. McBride
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2017)
Article
Immunology
Shubhajit Mitra, Paige S. Dunphy, Seema Das, Bing Zhu, Tian Luo, Jere W. McBride
INFECTION AND IMMUNITY
(2018)
Article
Microbiology
Tian Luo, Shubhajit Mitra, Jere W. McBride
Article
Immunology
Paige Selvy Dunphy, Tian Luo, Jere W. McBride
INFECTION AND IMMUNITY
(2014)
Article
Immunology
Paige Selvy Dunphy, Tian Luo, Jere W. McBride
MICROBES AND INFECTION
(2013)
Article
Immunology
Tian Luo, Jignesh G. Patel, Xiaofeng Zhang, David H. Walker, Jere W. McBride
Article
Microbiology
Madison R. Rogan, LaNisha L. Patterson, Caitlan D. Byerly, Tian Luo, Slobodan Paessler, Veljko Veljkovic, Bethany Quade, Jere W. McBride
Summary: This study reveals that the protein TRP120 of Ehrlichia chaffeensis interacts directly with components of the Wnt receptor complex, influencing pathway activity and promoting infection. This finding highlights a unique mechanism of bacterial mimicry of Wnt pathway ligands and provides insights into potential antimicrobial therapeutic targets.
Article
Parasitology
Andreia Lima Tome Melo, Tian Luo, Xiaofeng Zhang, Livia Saab Muraro, Nathalia Assis Pereira, Alejandro Cabezas-Cruz, Filipe Dantas-Torres, Jere W. McBride, Daniel Moura de Aguiar
Summary: This study found that multiple Ehrlichia agents infect dogs in Brazil, including Ehrlichia canis and Ehrlichia minasensis. Through serum testing and antibody analysis, it was discovered that some dogs had been infected with E. minasensis.
Article
Immunology
Tian Luo, Jignesh G. Patel, Xiaofeng Zhang, David H. Walker, Jere W. McBride
Summary: The immunomes of Ehrlichia chaffeensis and Ehrlichia canis are dominated by species-specific conformational antibody epitopes, with certain proteins identified as immunodominant and most proteins containing transmembrane domains. These findings enhance understanding of host-Ehrlichia immune responses and contribute to the development of effective vaccines against ehrlichiosis.
INFECTION AND IMMUNITY
(2021)
Review
Immunology
Duc-Cuong Bui, Tian Luo, Jere W. McBride
Summary: Bacteria in the Rickettsiales order, which are transmitted by arthropod vectors, cause life-threatening infections in humans and animals. The Type 1 Secretion System (T1SS) and associated effectors play important roles in evading host immune responses and promoting intracellular survival. This review summarizes current knowledge on the roles of T1SS effectors in Rickettsiales infections and explores newly identified immunoreactive proteins as potential targets for a protective immune response.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Immunology
Taslima T. Lina, Tian Luo, Thangam-Sudha Velayutham, Seema Das, Jere W. McBride
INFECTION AND IMMUNITY
(2017)