Article
Biochemistry & Molecular Biology
Irshad Sharafutdinov, Nicole Tegtmeyer, Mathias Musken, Steffen Backert
Summary: The study found that the Campylobacter bacteria can disrupt cellular junctions and allow non-invasive microbiota to transmigrate through the paracellular route. This discovery has important implications for understanding Campylobacter-associated diseases such as inflammatory bowel disease.
Review
Biotechnology & Applied Microbiology
Leon Kemper, Andreas Hensel
Summary: Campylobacter jejuni, causing severe enteritis, has unique features and adapts effectively to environmental conditions. The review summarizes the strategies of C. jejuni for host-pathogen interaction, providing insights for future drug development.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Microbiology
Andrea Laconi, Ilenia Drigo, Nicola Palmieri, Lisa Carraro, Elena Tonon, Raffaela Franch, Luca Bano, Alessandra Piccirillo
Summary: The study revealed that Campylobacter isolates from chicken livers possessed a higher number of virulence-associated genes, which might contribute to the development of pathological lesions in these isolates within the host.
VETERINARY MICROBIOLOGY
(2021)
Article
Immunology
Shiho Fukushima, Takaaki Shimohata, Yuri Inoue, Junko Kido, Takashi Uebanso, Kazuaki Mawatari, Akira Takahashi
Summary: Campylobacter jejuni infection activates the Rac1 signaling pathway to promote bacterial internalization, and LC3 plays a role in regulating the invasion signaling of C. jejuni, thereby participating in autophagy-mediated host immune response.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Fabia D. Lobo de Sa, Steffen Backert, Praveen K. Nattramilarasu, Soraya Mousavi, Geoffrey Sandle, Stefan Bereswill, Markus M. Heimesaat, Jorg-Dieter Schulzke, Roland Buecker
Summary: The study found that Vitamin D can improve the intestinal barrier dysfunction caused by Campylobacter infection, reducing bacterial transmigration.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Immunology
Jiayun Gu, Yan Lin, Zhichao Wang, Qicong Pan, Guohua Cai, Qigai He, Xiaojuan Xu, Xuwang Cai
Summary: This study elucidates the molecular mechanism by which CDT induces pyroptosis via the ROS/caspase-9/caspase-3/GSDME signaling pathway.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Biology
Stephanie Peters, Ben Pascoe, Zuowei Wu, Sion C. Bayliss, Ximin Zeng, Adam Edwinson, Sakteesh Veerabadhran-Gurunathan, Selina Jawahir, Jessica K. Calland, Evangelos Mourkas, Robin Patel, Terra Wiens, Marijke Decuir, David Boxrud, Kirk Smith, Craig T. Parker, Gianrico Farrugia, Qijing Zhang, Samuel K. Sheppard, Madhusudan Grover
Summary: Researchers used whole-genome sequencing and phenotypic analysis to identify potential genetic factors linked to irritable bowel syndrome caused by Campylobacter jejuni infection. They found that specific genetic variations in bacterial stress response, adhesion protein, and core biosynthetic pathway genes may contribute to the development of post-infection irritable bowel syndrome (PI-IBS). The study demonstrated that certain Campylobacter genotypes exhibit increased virulence in vitro, shedding light on the complex host-pathogen interactions underlying this condition.
COMMUNICATIONS BIOLOGY
(2021)
Article
Microbiology
Andrea Laconi, Roberta Tolosi, Ilenia Drigo, Luca Bano, Alessandra Piccirillo
Summary: This study assessed the ability of C. jejuni and C. coli strains isolated from diseased poultry to form biofilm. The results showed that C. jejuni strains were classified as no, weak, and moderate biofilm producers, while all C. coli strains were weak producers. Genes involved in chemotaxis, adhesion, and invasion were present in all C. jejuni strains. Further investigations are needed to better understand the genetic determinants involved in extra-intestinal Campylobacter biofilm formation.
VETERINARY MICROBIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Sholpan Omarova, Karem Awad, Verena Moos, Christoph Puening, Greta Goelz, Jorg-Dieter Schulzke, Roland Buecker
Summary: This study observed patients with persistent PI-IBS symptoms after C. jejuni infection and found that the epithelial resistance of the colon epithelium was unaltered, but the permeability of HRP increased and some inflammation-related markers were elevated. Therefore, targeting these mechanisms caused by the infection may help reduce sequelae such as PI-IBS.
Review
Gastroenterology & Hepatology
Viktoria Hentschel, Thomas Seufferlein, Milena Armacki
Summary: Maintaining the integrity of the mucosal barrier is crucial for intestinal homeostasis and overall health, depending on a balanced signaling system between the intestinal epithelium and other cell populations. Disruptions to this communication may lead to sustained weakening of the intestinal barrier function, causing inflammation, tissue damage, and dysbiosis. Organoid-derived intestinal coculture models provide a convenient tool for studying these pathophysiological events and exploring interactions between cell populations to focus on intestinal barrier recovery and stabilization.
AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY
(2021)
Article
Cell Biology
Geunhye Hong, Cadi Davies, Zahra Omole, Janie Liaw, Anna D. D. Grabowska, Barbara Canonico, Nicolae Corcionivoschi, Brendan W. W. Wren, Nick Dorrell, Abdi Elmi, Ozan Gundogdu
Summary: Campylobacter jejuni is the main bacterial cause of worldwide foodborne gastroenteritis and its interaction with the defense mechanisms of human intestinal epithelial cells (IECs) is not well understood. This study shows that C. jejuni regulates intracellular and extracellular ROS production in IECs and downregulates the expression of key ROS-generating enzyme NADPH oxidase, as well as antioxidant defense genes CAT and SOD1. Inhibition of NADPH oxidase reduces C. jejuni's ability to interact and survive within IECs. These findings provide mechanistic insight into how C. jejuni modulates the defense machinery of IECs.
CELLULAR MICROBIOLOGY
(2023)
Article
Microbiology
Pengxiang Li, Yifang Cui, Fangfang Guo, Jiahui Guo, Xiaoya Cao, Jun Lin, Baoan Ding, Fuzhou Xu
Summary: This study aimed to characterize the chicken host defense peptides (HDPs) responses to Campylobacter jejuni (C. jejuni) infection. In vitro experiments showed that C. jejuni infection suppressed the expression of certain HDPs but increased the expression of others. In vivo experiments in chickens demonstrated that C. jejuni infection initially up-regulated the expression of HDPs, but this expression decreased over time. The findings suggest that C. jejuni evades the chicken immune system by suppressing the expression of chicken HDPs.
VETERINARY MICROBIOLOGY
(2023)
Article
Gastroenterology & Hepatology
Yosra A. Helmy, Issmat I. Kassem, Gireesh Rajashekara
Summary: This study evaluated the impact of E. coli Nissle 1917 (EcN) on Campylobacter jejuni infections in HT-29 cells. Pre-treatment with EcN reduced C. jejuni invasion and intracellular survival in the cells, possibly through induction of anti-inflammatory cytokines and activation of anti-apoptotic signaling. EcN also positively affected gene expression related to cellular maintenance, growth, development, and immunity, modulating key signaling pathways involved in innate immunity.
Article
Agriculture, Dairy & Animal Science
Luis R. Munoz, Matthew A. Bailey, James T. Krehling, Dianna V. Bourassa, Ruediger Hauck, Wilmer J. Pacheco, Bernardo Chaves-Cordoba, Kaicie S. Chasteen, Aidan A. Talorico, Cesar Escobar, Andrea Pietruska, Ken S. Macklin
Summary: A study was conducted to assess the effects of a dietary yeast cell wall (YCW) with and without a Campylobacter jejuni (CJ) challenge. The results showed that the addition of YCW did not have an impact on broiler performance, innate immune response, cecal colonization, carcass yield, or CJ prevalence after processing.
Article
Immunology
Wayne T. Muraoka, Anna A. Korchagina, Qingqing Xia, Sergey A. Shein, Xi Jing, Zhao Lai, Korri S. Weldon, Li-Ju Wang, Yidong Chen, Lawrence W. Kummer, Markus Mohrs, Eric Vivier, Ekaterina P. Koroleva, Alexei V. Tumanov
Summary: Research showed that IFN-γ-producing ILCs can promote intestinal pathology in a mouse model of enterocolitis caused by Campylobacter jejuni, arising from RORγt(+) progenitors. This highlights the importance of these ILCs in intestinal immune regulation.
MUCOSAL IMMUNOLOGY
(2021)
Article
Food Science & Technology
Igori Balta, Mark Linton, Laurette Pinkerton, Carmel Kelly, Patrick Ward, Lavinia Stef, Ioan Pet, Adina Horablaga, Ozan Gundogdu, Nicolae Corcionivoschi
Summary: This study found that using a mixture of natural antimicrobials can significantly reduce the ability of Campylobacter coli to adhere to chicken skin and carcasses, with a stronger effect on T6SS positive isolates. In vitro experiments also showed that at a concentration of 0.5% A3001, it can impact the expression of the hcp gene in T6SS positive isolates.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2021)
Editorial Material
Microbiology
Nicolae Corcionivoschi, Stuart A. Thompson, Ozan Gundogdu
FRONTIERS IN MICROBIOLOGY
(2021)
Review
Food Science & Technology
Igori Balta, Mark Linton, Laurette Pinkerton, Carmel Kelly, Lavinia Stef, Ioan Pet, Ducu Stef, Adriana Criste, Ozan Gundogdu, Nicolae Corcionivoschi
Summary: The increasing antibiotic resistance in Campylobacter species highlights the need for novel antimicrobials that can inhibit virulence without causing resistance or host toxicity. Understanding the antibacterial potency and biological mechanisms of natural antimicrobials is crucial for combating bacterial virulence factors. Further research is necessary to evaluate the effectiveness of plant, animal, bacteria, and marine-derived antimicrobials as promising alternatives to antibiotics.
Review
Biochemistry & Molecular Biology
Gratiela Gradisteanu Pircalabioru, Nicolae Corcionivoschi, Ozan Gundogdu, Mariana-Carmen Chifiriuc, Luminita Gabriela Marutescu, Bogdan Ispas, Octavian Savu
Summary: The global incidence of T1DM is increasing and research suggests that the gut microbiota may play a role in its pathogenesis. Interventions such as probiotics may help prevent and treat T1DM, emphasizing the importance of further clinical trials to confirm their efficacy and safety.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Oncology
Katie Wang, Kento Nakano, Naghmeh Naderi, Mona Bajaj-Elliott, Afshin Mosahebi
Summary: The involvement of skin microbiota in the development of breast diseases is still unclear, but it has been suggested that skin commensals may play a role in tissue homeostasis and contribute to various breast pathologies. Further research is needed to explore the potential of antimicrobials/probiotics as a treatment option for breast diseases.
Article
Microbiology
Fauzy Nasher, Aidan J. Taylor, Abdi Elmi, Burhan Lehri, Umer Z. Ijaz, Dave Baker, Richard Goram, Steven Lynham, Dipali Singh, Richard Stabler, David J. Kelly, Ozan Gundogdu, Brendan W. Wren
Summary: RrpA and RrpB play important roles in the survival of Campylobacter jejuni under redox stress. RrpA positively regulates mdaB, which contributes to protection from redox stress, while RrpB negatively regulates cj1555c (renamed nfrA), which encodes a flavin reductase.
JOURNAL OF BACTERIOLOGY
(2022)
Article
Microbiology
Xiaofei Li, Qinwen Chai, Lina Zheng, Pingyu Huang, Ozan Gundogdu, Xinan Jiao, Yuanyue Tang, Jinlin Huang
Summary: This study demonstrated the critical role of the B and N domains of FlhF protein in flagellar assembly in Campylobacter jejuni. Point mutations in these domains caused abnormal flagella, disrupted GTPase activity and polar targeting of FlhF, and significantly affected flagella gene expression and transcription.
MICROBIOLOGICAL RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Igori Balta, Lavinia Stef, Eugenia Butucel, Gratiela Gradisteanu Pircalabioru, Adelina Venig, Patrick Ward, Myriam Deshaies, Ioan Pet, Ducu Stef, Osman Y. Koyun, Todd R. Callaway, Ozan Gundogdu, Nicolae Corcionivoschi
Summary: The study found that natural antimicrobials have a significant inhibitory effect on Nematopsis messor infections in shrimp primary intestinal cells, restoring gut integrity and enhancing gut immunity. This was further confirmed in a shrimp challenge test, indicating its in vivo antioxidant function.
Article
Food Science & Technology
Igori Balta, Eugenia Butucel, Lavinia Stef, Ioan Pet, Gratiela Gradisteanu-Pircalabioru, Carmen Chifiriuc, Ozan Gundogdu, David McCleery, Nicolae Corcionivoschi
Summary: This review summarizes the use of probiotics in reducing Campylobacter levels in poultry and decreasing the pathogen's virulence in avian and human hosts. Probiotics have been found to achieve this through immunomodulation and anti-pathogenic mechanisms.
FOODBORNE PATHOGENS AND DISEASE
(2022)
Article
Biochemistry & Molecular Biology
Gratiela Gradisteanu Pircalabioru, Janie Liaw, Ozan Gundogdu, Nicolae Corcionivoschi, Iuliana Ilie, Luciana Oprea, Madalina Musat, Mariana-Carmen Chifiriuc
Summary: This study aims to investigate the role of gut microbiota in the development of metabolic syndrome (MetSyn) and type 2 diabetes mellitus (T2DM). The findings show that MetSyn patients have reduced microbial diversity and loss of butyrate producers in their gut microbiome. Furthermore, T2DM patients with MetSyn exhibit distinct gut microbiota signatures. The study also highlights the impact of metformin treatment on the gut microbiome.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Microbiology
Umer Zeeshan Ijaz, Ozan Gundogdu, Ciara Keating, Miriam van Eekert, Walter Gibson, Julian Parkhill, Faraji Abilahi, Benard Liseki, Viet-Anh Nguyen, Steven Sudgen, Christopher Quince, Jeroen H. J. Ensink, Belen Torondel, Alan W. Walker
Summary: This study conducted a spatial analysis of 35 Tanzanian pit latrines to investigate the bacteria and environmental factors associated with fill-up rates. The research found a linear gradient of beta diversity with increasing pit latrine sample depth, indicating a shift in microbial community structure. Factors such as pH, volatile solids, volatile fatty acids, and phosphate were identified as strongly correlated with fill-up rates. The study enhances understanding of the microbiota within pit latrines and provides new insights for future research on the decomposition process.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Cell Biology
Geunhye Hong, Cadi Davies, Zahra Omole, Janie Liaw, Anna D. D. Grabowska, Barbara Canonico, Nicolae Corcionivoschi, Brendan W. W. Wren, Nick Dorrell, Abdi Elmi, Ozan Gundogdu
Summary: Campylobacter jejuni is the main bacterial cause of worldwide foodborne gastroenteritis and its interaction with the defense mechanisms of human intestinal epithelial cells (IECs) is not well understood. This study shows that C. jejuni regulates intracellular and extracellular ROS production in IECs and downregulates the expression of key ROS-generating enzyme NADPH oxidase, as well as antioxidant defense genes CAT and SOD1. Inhibition of NADPH oxidase reduces C. jejuni's ability to interact and survive within IECs. These findings provide mechanistic insight into how C. jejuni modulates the defense machinery of IECs.
CELLULAR MICROBIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Augustin Tshibaka Kabongo, Rajib Acharjee, Takaya Sakura, Gloria Mavinga Bundutidi, Endah Dwi Hartuti, Cadi Davies, Ozan Gundogdu, Kiyoshi Kita, Tomoo Shiba, Daniel Ken Inaoka
Summary: Campylobacter jejuni infection is a serious global health threat, and the increasing incidence and antibiotic resistance have called for the development of new drugs targeting this bacterial infection. Malate:quinone oxidoreductase (MQO) is an attractive drug target as it is essential for the survival of several bacteria and parasites but not conserved in mammals. In this study, the researchers purified and analyzed MQO from C. jejuni, showing that it can be inhibited by ferulenol and embelin, which also inhibit the growth of C. jejuni in vitro.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2023)
Article
Multidisciplinary Sciences
Israr Aziz, Zobia Noreen, Umer Zeeshan Ijaz, Ozan Gundogdu, Muhammad Haroon Hamid, Nazir Muhammad, Abdullah Khan, Habib Bokhari
Summary: Stunted growth is a global challenge in low- and middle-income countries for children under age 5. This study investigated the association between pathogenic E. coli strains and the gut microbiota of stunted children. Stool samples from 64 children were analyzed for E. coli characterization and microbial community profiling. The study found that different pathotypes of E. coli were present, with the most prevalent being EPEC. Microbial diversity and certain bacteria were found to be altered in stunted children.
SCIENTIFIC REPORTS
(2023)