Article
Biochemistry & Molecular Biology
Alexandra Teixeira, Ines Loureiro, Johnny Lisboa, Pedro N. Oliveira, Jorge E. Azevedo, Nuno M. S. dos Santos, Ana do Vale
Summary: Phdp is a Gram-negative fish pathogen that causes significant economic losses in aquaculture. This study found that Phdp secretes large amounts of OMVs during in vitro culture and infection, which protect Phdp cells from fish antimicrobial peptides. Vaccination with OMVs induced the production of anti-Phdp antibodies and provided partial protection against infection in sea bass.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Microbiology
Alba V. Barca, Ana Vences, Mateus S. Terceti, Ana do Vale, Carlos R. Osorio
Summary: This study investigated the transcriptional response of the facultative marine pathogen Photobacterium damselae subsp. damselae to changes in salinity. It was found that low salinity conditions triggered a virulence gene expression profile, while growth at high salinity upregulated genes involved in energy production and metabolism. This study enhances our understanding of the salinity-responsive adaptations of a generalist marine pathogen.
Article
Immunology
Feng-Jie Su, Meei-Mei Chen
Summary: The oral biofilm vaccine demonstrated a protective effect against Photobacterium damselae subsp. damselae, increasing phagocytosis and improving immune response in infected fish, resulting in reduced cumulative mortality rate.
Article
Fisheries
Jiasong Xie, Han Mei, Shan Jin, Lingfei Bu, Xinyi Wang, Chunlin Wang, Qingsong Zhao, Rongrong Ma, Suming Zhou
Summary: In May 2017, a massive mortality of mud crab Scylla paramamosain occurred in a farm in Sanmen, Zhejiang Province, China, caused by Photobacterium damselae subsp. damselae infection. The QX175062 isolate was lethal to mud crabs with LD(50) value of 3.63 x 10(1) CFU/g, highlighting the importance of developing rapid diagnostic methods and prevention measures for economic losses in mud crab aquaculture due to P. damselae subsp. damselae infection.
Article
Biochemistry & Molecular Biology
Saqr Abushattal, Ana Vences, Carlos R. Osorio
Summary: The unstable plasmid pPHDPT3, encoding a type III secretion system, is a major virulence factor of the marine bacterium Photobacterium damselae subsp. piscicida (Pdp). However, pPHDPT3 undergoes curing upon in vitro cultivation, resulting in a significant reduction in Pdp's virulence for fish.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Fisheries
Aki Nishihara, Natsuki Morimoto, Takechiyo Sumiyoshi, Shinya Yasumoto, Masakazu Kondo, Tomoya Kono, Masahiro Sakai, Jun-ichi Hikima
Summary: The Ivy gene in the plasmid of Pdp may help the bacteria evade lysozyme-mediated lysis during interaction with an animal host. This study found that Ivy-Pdp is a temperature- and pH-stable lysozyme inhibitor, and it inhibits the lytic activity of lysozyme involved in host biophylaxis.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Paulo Santos, Diogo Peixoto, Ines Ferreira, Ricardo Passos, Pedro Pires, Marco Simoes, Pedro Pousao-Ferreira, Teresa Baptista, Benjamin Costas
Summary: Photobacteriosis is a bacterial disease that affects various marine species globally, leading to significant economic losses. Despite studies on pathogen virulence and resistance, information on host defense mechanisms post-infection remains limited. This study focused on innate immune responses of farmed juvenile gilthead seabream after infection with Photobacterium damselae subsp. piscicida, revealing marked changes in blood parameters and gene expression related to inflammation and phagocytic activity. Further research on different fish species and pathogens could help identify health biomarkers for disease prevention in aquaculture.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Food Science & Technology
Athanasios Lattos, Ilias Chaligiannis, Dimitrios Papadopoulos, Ioannis A. Giantsis, Evanthia I. Petridou, George Vafeas, Alexandra Staikou, Basile Michaelidis
Summary: The study evaluated the risk of consuming raw bivalves from the two largest seafood markets in Thessaloniki, Greece, and found the presence of harmful microbes, but most of them did not exceed the European Union thresholds. Additionally, non-target pathogenic microbes were detected in the samples, with Vibrio and a protozoan parasite identified.
Article
Food Science & Technology
Ines Lua Freitas, Alexandra Teixeira, Ines Loureiro, Johnny Lisboa, Aurelia Saraiva, Nuno Miguel Simoes dos Santos, Ana do Vale
Summary: Photobacterium damselae subsp. piscicida (Phdp) is a Gram-negative bacterium that infects various marine fish species. Its virulence factor, AIP56, induces apoptosis of sea bass phagocytes during infection. This study found that sea bream is resistant to AIP56 toxicity due to inefficient internalization of the toxin. In contrast, sea bass is more susceptible to AIP56-induced lethality. These findings highlight the different effects of AIP56 in sea bass and sea bream.
Article
Fisheries
Antonio Vallecillos, Elena Chaves-Pozo, Marta Arizcun, Ruben Perez, Juan M. Afonso, Concepcion Berbel, Jaume Perez-Sanchez, Emilio Maria-Dolores, Eva Armero
Summary: A challenge test for Photobacterium damselae subsp. piscicida resistance was conducted in two populations of gilthead seabream, revealing moderate heritabilities for body weight and disease resistance, and low heritability for days to death. Genetic correlations were high and positive between body weight and disease survival, and tended to be positive with immune markers. Selective breeding programs may consider incorporating immune markers like peroxidase activity, along with performance traits, to breed disease-resistant fish.
Article
Immunology
Po-Tsang Lee, Fan-Hua Nan, Po-Yu Chiu, Chung-Chih Tseng, Meng-Chou Lee
Summary: This study examined the effects of Sarcodia suiae water extract (SSWE) on cobia, and found that SSWE treatment can enhance the fish's immune response against pathogens and has the potential to be used as an immunostimulant and vaccine adjuvant for fish.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Immunology
Daniella-Mari White, Michail-Aggelos Valsamidis, Georgios D. Kokkoris, Vasileios Bakopoulos
Summary: Infectious diseases in aquaculture can have negative impacts on the fish farming industry, consumers, and the environment. This study aimed to investigate the effect of water temperature and challenge route on hemocyte phagocytosis in octopods after experimental challenge with two different pathogens. The results suggest that temperature, time post-challenge, route of challenge, and pathogen may influence the phagocytosis ability of hemocytes.
MICROBIAL PATHOGENESIS
(2023)
Article
Fisheries
Yongxiang Yu, Yingeng Wang, Meijie Liao, Linni Shi, Yongjie Li, Bin Li, Xiaojun Rong, Chunyuan Wang, Zheng Zhang
Summary: This study investigated the spleen transcriptome response of black rockfish infected with Photobacterium damselae subsp. damselae, revealing the involvement of immune-related genes in the pathogenesis process over time. The expression profiles of immune-related genes were found to correlate with disease progression, with more immune-related signal pathways and genes involved in the early stage of infection. The findings provide valuable insights into the molecular responses of black rockfish to photobacteriosis.
AQUACULTURE RESEARCH
(2022)
Article
Genetics & Heredity
Laura Baseggio, Oleksandra Silayeva, Nicky Buller, Matt Landos, Jan Englestadter, Andrew C. Barnes
Summary: Despite recent technological advances, complete assembly of multi-chromosome genomes of Vibrionaceae remains challenging. This study successfully sequenced, closed, and curated the genomes of two strains of Photobacterium damselae subsp. piscicida isolated in Australia using a combination of long Oxford Nanopore MinION reads and short Illumina reads. Phylogenetic analysis revealed closer relationship of Australian strains to Asian and American strains rather than to European ones. Host specialization and adaptive evolution to fish in P. damselae subsp. piscicida are ongoing processes mediated by transposable elements and plasmids. Identification of potential virulence determinants like the chorismate mutase gene and the transfer-messenger RNA gene ssrA provides new insights into the evolution of this fish pathogen.
MICROBIAL GENOMICS
(2021)
Article
Fisheries
Muthusamy Petchimuthu, Mulloorpeedikayil Rosalind George, Kollanoor Riji John, Vanniyaraj Santhana Kumar
Summary: The study found a high prevalence of antimicrobial resistance in Photobacterium damselae subsp. damselae isolated from fish in the southeast coast of India, indicating strong pathogenic potential to cage-cultured fish.
AQUACULTURE RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Marta A. Lages, M. Carmen de la Fuente, Lucia Ageitos, Diana Martinez-Matamoros, Jaime Rodriguez, Miguel Balado, Carlos Jimenez, Manuel L. Lemos
Summary: The study confirmed the importance of FrpA in mediating ferri-Pcb uptake by analyzing mutants lacking FrpA. Additionally, the authors evaluated the internalization ability of several Pcb analogues, and concluded that maintaining a hydroxyl group at position C-13 is crucial for chelating Fe3+ ions.
JOURNAL OF BIOLOGICAL INORGANIC CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Saqr Abushattal, Ana Vences, Carlos R. Osorio
Summary: The unstable plasmid pPHDPT3, encoding a type III secretion system, is a major virulence factor of the marine bacterium Photobacterium damselae subsp. piscicida (Pdp). However, pPHDPT3 undergoes curing upon in vitro cultivation, resulting in a significant reduction in Pdp's virulence for fish.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Javier Cisneros-Sureda, Diego Rey-Varela, Jaime Rodriguez, Miguel Balado, Manuel L. Lemos, Carlos Jimenez
Summary: This study reports the design and synthesis of a new fluorescent probe based on amonabactins, which can label Aeromonas bacteria through their specific transport proteins. The probe also has the potential for selective labeling of other Aeromonas species and can be used for detection of Aeromonas in water and fish farming samples.
JOURNAL OF INORGANIC BIOCHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Marta A. Lages, Lucia Ageitos, Jaime Rodriguez, Carlos Jimenez, Manuel L. Lemos, Miguel Balado
Summary: This study identified key functions encoded by irp-HPI that are necessary for piscibactin production and transport. Irp4 was found to be an essential thioesterase for piscibactin release, while Irp8 was identified as a crucial protein for piscibactin secretion. FrpBC was shown to be responsible for the internalization of ferri-piscibactin. These findings provide valuable insights for the design of novel antimicrobials against pathogenic vibrios.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Inorganic & Nuclear
M. Carmen de la Fuente, Lucia Ageitos, Marta A. Lages, Diana Martinez-Matamoros, Abel M. Forero, Miguel Balado, Manuel L. Lemos, Jaime Rodriguez, Carlos Jimenez
Summary: This study successfully performed stereo-selective total synthesis of Pcb analogues, which are siderophores produced by pathogenic Gram-negative bacteria. The replacement of the acid-sensitive α-methylthiazoline moiety with a more stable thiazole ring and varying the OH group configuration at C-13 resulted in different metal coordination abilities. The study also confirmed the stereochemistry of the Ga3+ complex and proposed the scaffold's potential use in designing new chelating agents or antibacterials.
INORGANIC CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Diego Rey-Varela, Miguel Balado, Manuel L. Lemos
Summary: Aeromonas salmonicida subsp. salmonicida (A. salmonicida), a Gram-negative bacterium causing furunculosis in fish, produces siderophores acinetobactin and amonabactins to extract iron, but the regulation pathways and conditions for their production are not clear.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Immunology
M. Pilar Escribano, Miguel Balado, Alicia E. Toranzo, Manuel L. Lemos, Beatriz Magarinos
Summary: This study analyzed the prevalence of extracellular proteolytic and lipolytic activities related to virulence in Tenacibaculum maritimum strains. They found a great intra-specific heterogeneity in enzymatic capacity, particularly within serotype O4. It was also discovered that OMVs, specifically enriched in certain proteins, played a key role in virulence and biofilm formation.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Microbiology
Alba V. Barca, Ana Vences, Mateus S. Terceti, Ana do Vale, Carlos R. Osorio
Summary: This study investigated the transcriptional response of the facultative marine pathogen Photobacterium damselae subsp. damselae to changes in salinity. It was found that low salinity conditions triggered a virulence gene expression profile, while growth at high salinity upregulated genes involved in energy production and metabolism. This study enhances our understanding of the salinity-responsive adaptations of a generalist marine pathogen.
Article
Microbiology
Marta A. Lages, Ana do Vale, Manuel L. Lemos, Miguel Balado
Summary: This study found that PbtA not only interacts with irp-HPI promoters but also regulates the expression of many genes in V. anguillarum, including genes involved in metabolism, immunity, and virulence. These results are significant for understanding the regulatory mechanisms of irp-HPI island and its pathogenicity.
Article
Immunology
A. Rodriguez-Pedrouzo, J. Cisneros-Sureda, D. Martinez-Matamoros, D. Rey-Varela, M. Balado, J. Rodriguez, M. L. Lemos, M. Folgueira, C. Jimenez
Summary: Zebrafish is an excellent model for studying bacterial infections in fish. This study used zebrafish as a model to investigate the infection process of Aeromonas salmonicida in fish and evaluated the use of fluorescence microscopy to track the infection in vivo.
MICROBIAL PATHOGENESIS
(2023)