Article
Agriculture, Dairy & Animal Science
Jian Tu, Dandan Fu, Yi Gu, Ying Shao, Xiangjun Song, Mei Xue, Kezong Qi
Summary: APEC is the responsible pathogen for colibacillosis in poultry, and the role of YgeK in APEC has not been reported. This study found that YgeK plays a crucial role in APEC virulence by affecting flagellar formation, bacterial motility, serum sensitivity, and adhesion ability. The results contribute to understanding the function of the ETT2 cluster in APEC and clarify its pathogenic mechanism.
Review
Biochemistry & Molecular Biology
Babak Pakbin, Wolfram M. Bruck, John W. A. Rossen
Summary: Escherichia coli are versatile microorganisms found in the normal intestinal microbiota of humans and animals. They can become emerging human pathogens by acquiring genes encoding virulence factors. Nine enteric E. coli pathotypes have been characterized, utilizing various virulence factors to cause intestinal and extraintestinal diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Microbiology
Camille Lucas, Sabine Delannoy, Catherine Schouler, Rozenn Souillard, Laetitia Le Devendec, Pierrick Lucas, Alassane Keita, Patrick Fach, Julie Puterflam, Stephanie Bougeard, Isabelle Kempf
Summary: This study identified relevant genetic markers associated with avian pathogenic E. coli (APEC) using high-throughput PCR and a machine learning procedure, which is crucial for better control of avian colibacillosis.
VETERINARY MICROBIOLOGY
(2022)
Article
Microbiology
Xuhua Chen, Wenxing Liu, Huoming Li, Shigan Yan, Fengwei Jiang, Wentong Cai, Ganwu Li
Summary: Avian pathogenic Escherichia coli (APEC) show great genetic diversity with various virulence-associated genes that could serve as markers. Different virulence-associated gene clusters are significantly associated with specific phylogroups of APEC, suggesting a distinct distribution pattern. This study provides insights into the evolution and pathogenesis of APEC, potentially leading to the development of new diagnostic tools.
VETERINARY MICROBIOLOGY
(2021)
Article
Veterinary Sciences
Lei Yin, Baoyan Cheng, Jian Tu, Ying Shao, Xiangjun Song, Xiaocheng Pan, Kezong Qi
Summary: This study investigates the role of transcriptional regulator YqeH in the pathogenicity of avian pathogenic Escherichia coli (APEC). The results show that the deletion of YqeH leads to reduced motility and biofilm formation, resulting in decreased virulence of APEC. Additionally, YqeH regulates the transcriptional levels of genes associated with flagellum, biofilm, and virulence, impacting the pathogenicity of APEC.
VETERINARY RESEARCH
(2022)
Article
Microbiology
Zhuohao Wang, Xiangkuan Zheng, Genglin Guo, Zimeng Hu, Jinfeng Miao, Yongyi Dong, Zhengjun Xu, Qingan Zhou, Xiankai Wei, Xiangan Han, Yuqing Liu, Wei Zhang
Summary: Among Avian pathogenic Escherichia coli (APEC) serogroups in China, O145 may be emerging as a predominant serogroup. This serogroup has been overlooked but may have stronger pathogenicity. Genomic analysis revealed possible reasons for its prevalence. This newly predominant serogroup should be given more attention, not limited to the three traditional APEC serogroups.
VETERINARY MICROBIOLOGY
(2022)
Article
Microbiology
Marianne Nicolas, Angelina Trotereau, Antoine Culot, Arshnee Moodley, Robert Atterbury, Jeroen Wagemans, Rob Lavigne, Philippe Velge, Catherine Schouler
Summary: Colibacillosis, the most common bacterial disease affecting poultry, is primarily treated with antibiotics. However, the rise of multidrug-resistant avian-pathogenic Escherichia coli (APEC) necessitates the exploration of alternative treatments, such as phage therapy. This study isolates and characterizes 19 genetically diverse coliphages, and demonstrates their efficacy in controlling APEC infections in ovo.
MICROBIOLOGY SPECTRUM
(2023)
Article
Microbiology
Dezhi Li, Wei Liang, Qingyue Hu, Jianluan Ren, Feng Xue, Qing Liu, Fang Tang
Summary: This study investigated the role of spontaneous induction prophages in avian pathogenic Escherichia coli (APEC) and found that phi458 promoted biofilm formation and decreased virulence by repressing virulence genes.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Microbiology
Courtney A. Fancher, Hudson T. Thames, Mary Gates Colvin, Mercedes Smith, Alyssa Easterling, Nikhil Nuthalapati, Li Zhang, Aaron Kiess, Thu T. N. Dinh, Anuraj Theradiyil Sukumaran
Summary: The study investigated the prevalence and virulence of E. coli in antibiotic-free broiler farms, determining that there is a high prevalence of virulent E. coli, especially during the spring season. Antimicrobial resistance was highest for tetracycline, and serogroups O8 and O78 were most frequent in virulent E. coli.
MICROBIOLOGY SPECTRUM
(2021)
Article
Veterinary Sciences
Dosseh Jean Apotre Afayibo, Hong Zhu, Beibei Zhang, Lan Yao, Hosny Ahmed Abdelgawad, Mingxing Tian, Jingjing Qi, Yali Liu, Shaohui Wang
Summary: This study identified the serotypes, phylogenetic groups, virulence genes distribution, and antibiotic resistance profiles of APEC isolates in eastern China. The APEC isolates were found to carry a variety of virulence genes and exhibit multi-antibiotic resistance profiles.
VETERINARY SCIENCES
(2022)
Review
Microbiology
Adrianna Aleksandrowicz, Muhammad Moman Khan, Katarzyna Sidorczuk, Mateusz Noszka, Rafal Kolenda
Summary: APEC, a common pathogen in poultry, relies on various adhesins to evade host defense mechanisms and cause infection. Understanding these adhesins is essential for developing effective strategies to combat APEC infections.
VETERINARY MICROBIOLOGY
(2021)
Article
Food Science & Technology
Peili Wang, Jiaxiang Zhang, Yanfei Chen, Haoran Zhong, Heng Wang, Jianji Li, Guoqiang Zhu, Pengpeng Xia, Luying Cui, Jun Li, Junsheng Dong, Qingqing Gao, Xia Meng
Summary: The study confirmed that clbG is essential for the synthesis of genotoxin colibactin in APEC XM strain, and its deletion reduced the likelihood of APEC meningitis in mice.
Article
Immunology
Lan Yao, Yinli Bao, Jiangang Hu, Beibei Zhang, Zhiyang Wang, Xinyu Wang, Weiqi Guo, Di Wang, Jingjing Qi, Mingxing Tian, Yanqing Bao, Haihua Li, Shaohui Wang
Summary: This study isolated and purified a lytic avian pathogenic Escherichia coli (APEC) phage designated as PEC9 from chicken farm feces samples. PEC9 exhibited strong lytic activity and inhibitory ability against biofilm formation, and it could effectively inhibit bacterial growth. The therapeutic effects of PEC9 were confirmed in a mice model, with improved survival rate and reduced bacterial loads and organ lesions. These findings suggest that PEC9 may be a promising alternative to antibiotics in the control of APEC infection.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Multidisciplinary Sciences
Darby M. Newman, Nicolle L. Barbieri, Aline L. de Oliveira, Dajour Willis, Lisa K. Nolan, Catherine M. Logue
Summary: Colibacillosis caused by avian pathogenic Escherichia coli (APEC) is a devastating disease in poultry industry, causing significant economic losses. Study found that APEC strains commonly carry virulence-associated genes and ColV plasmid genes, form biofilms, and exhibit antimicrobial resistance.
Article
Veterinary Sciences
Lei Yin, Qianwen Li, Zeping Wang, Jian Tu, Ying Shao, Xiangjun Song, Xiaocheng Pan, Kezong Qi
Summary: The E. coli type III secretion system 2 (ETT2) plays a crucial role in the virulence of avian pathogenic E. coli (APEC), with ygeG identified as a chaperone protein that affects biofilm formation, cell adherence, invasion, and bacterial virulence. Deletion of ygeG also enhances bacterial virulence in chicken infection models, regulating genes involved in biofilm formation, outer membrane proteins, fimbrial genes, and virulence effector proteins.
RESEARCH IN VETERINARY SCIENCE
(2021)
Article
Infectious Diseases
Qi Feng, Timothy Frana, Catherine M. Logue, James D. McKean, Scott H. Hurd, Annette M. O'Connor, James S. Dickson, Shanyuan Zhu, Ganwu Li
Summary: This study compared the AMR patterns of Salmonella spp. in swine collected at arrival (fecal samples) at the abattoir and postslaughter (cecal samples), finding significant differences between the two. Therefore, the AMR profiles of Salmonella spp. postslaughter cannot be predicted from preslaughter samples. Further research considering the genetic diversity of isolates and statistical power of genotypic analysis is warranted to improve the performance of WGS-inferred antimicrobial susceptibility.
MICROBIAL DRUG RESISTANCE
(2021)
Article
Public, Environmental & Occupational Health
Atsushi Hinenoya, Xing-Ping Li, Ximin Zeng, Orhan Sahin, Rodney A. Moxley, Catherine M. Logue, Barbara Gillespie, Shinji Yamasaki, Jun Lin
Summary: This study investigated the prevalence and characteristics of E. albertii in US domestic and food animals, revealing chickens as an important reservoir for E. albertii. The isolated chicken E. albertii strains displayed multidrug resistance and phylogenetic closeness to human-derived strains. Further large-scale surveys in poultry production are warranted in the future.
ZOONOSES AND PUBLIC HEALTH
(2021)
Article
Multidisciplinary Sciences
Darby M. Newman, Nicolle L. Barbieri, Aline L. de Oliveira, Dajour Willis, Lisa K. Nolan, Catherine M. Logue
Summary: Colibacillosis caused by avian pathogenic Escherichia coli (APEC) is a devastating disease in poultry industry, causing significant economic losses. Study found that APEC strains commonly carry virulence-associated genes and ColV plasmid genes, form biofilms, and exhibit antimicrobial resistance.
Article
Microbiology
Nicolle Lima Barbieri, Ramon Loureiro Pimenta, Dayanne Araujo de Melo, Lisa K. Nolan, Miliane Moreira Soares de Souza, Catherine M. Logue
Summary: This study examined the presence of mcr genes in 107 E. coli isolates from production poultry in Brazil, revealing a high prevalence of mcr-1 in 62 isolates. Additional analyses such as plasmid replicon typing and antimicrobial resistance phenotype characterization were conducted for further understanding. The findings suggest that the high prevalence of mcr-1 in poultry could potentially pose a threat to antimicrobial resistance in the human food chain.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Immunology
Ximin Zeng, Huiwen Wang, Canghai Huang, Catherine M. Logue, Nicolle L. Barbieri, Lisa K. Nolan, Jun Lin
Summary: This study experimented with different vaccination regimens to determine efficient production of anti-Ent egg yolk IgY in chickens, and found that the novel Ent conjugate vaccine could induce strong, specific, and robust immune response in chickens, with potential for passive immune intervention against Gram-negative infections.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Catherine A. Chambers, Alexis S. Dadelahi, Charles R. Moley, Rachel M. Olson, Catherine M. Logue, Jerod A. Skyberg
Summary: This study investigated the role of NMEC strain-dependent P2X receptor (P2XR) signaling on the outcome of infection in neonatal mice. The results showed that alpha-hemolysin (HlyA)-expressing NMEC (HlyA(+)) induced robust P2XR-dependent macrophage cell death and P2XR inhibition increased mortality in neonatal mice infected with HlyA(+) NMEC. The findings also suggested that P2XR-dependent protection against HlyA(+) NMEC required macrophages.
Article
Multidisciplinary Sciences
Meaghan M. Young, Aline L. de Oliveira, Lisa K. Nolan, Nicolle L. Barbieri, Catherine M. Logue
Summary: This study aimed to investigate biofilm formation and related genes in avian pathogenic Escherichia coli (APEC). Through mutant screening and PCR analysis, nine genes associated with biofilm formation in APEC were identified, and four mutant genes showed significantly decreased biofilm formation ability. These findings are important for further understanding the mechanisms of APEC biofilm formation.
Article
Immunology
Huiwen Wang, Liu Cao, Catherine M. Logue, Nicolle L. Barbieri, Lisa K. Nolan, Jun Lin
Summary: Colibacillosis is a common and devastating infectious disease in poultry production. Universal vaccines are urgently needed to protect chickens from avian pathogenic Escherichia coli (APEC) infections. A novel Ent conjugate vaccine induced Ent-specific antibodies that inhibited the growth of diverse APEC strains.
Article
Veterinary Sciences
Roel Becerra, Jenny Nicholds, Karen Grogan, David French, Eric Shepherd, Catherine M. Logue
Summary: Spotty liver disease (SLD) has become an important cause of disease in egg-producing flocks, with organisms such as Campylobacter hepaticus and Campylobacter bilis causing liver lesions and negative impacts on egg production and mortality of valuable hens.
Article
Microbiology
Roel Becerra, Julia Ienes-Lima, Jenny Nicholds, Catherine M. Logue
Summary: Spotty liver disease (SLD) is a significant cause of disease in egg-producing flocks, with Campylobacter hepaticus and Campylobacter bilis being the implicated organisms. We report the complete genome sequence of C. hepaticus strain RBCL71delta and three additional strains isolated from a layer flock in Georgia, USA.
MICROBIOLOGY RESOURCE ANNOUNCEMENTS
(2023)
Article
Microbiology
Julia Ienes-Lima, Roel Becerra, Catherine M. M. Logue
Summary: Campylobacter hepaticus has re-emerged as a significant cause of disease in egg laying birds worldwide, leading to morbidity, mortality, and egg losses in the poultry industry. The disease is characterized by liver spots and histopathological analysis reveals necrotizing hepatitis. The re-emergence of C. hepaticus may be linked with housing practices and colonization in pasture raised birds.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Microbiology
Huiwen Wang, Catherine M. Logue, Lisa K. Nolan, Jun Lin
Summary: Colibacillosis, caused by avian pathogenic Escherichia coli (APEC), is a significant infectious disease in chickens and protecting young chickens from this disease is important. A novel enterobactin (Ent) conjugate vaccine was developed to induce high levels of anti-Ent immunoglobulin Y (IgY) in chicken serum, but the maternal immunity from the vaccinated hens did not provide significant protection against APEC challenge in the young chicks. Therefore, further optimization of the APEC infection model and improvement of the maternal immunization regimen are needed.
Article
Veterinary Sciences
Yu-Yang Tsai, Monique Franca, Alvin Camus, Lisa J. Stabler, Nicolle Barbieri, Catherine M. Logue
Summary: This study aimed to identify the bacteria commonly found in focal duodenal necrosis (FDN) lesions in table egg layers using histopathological analysis. The results showed no consistent bacterial species identified in FDN samples. However, analysis of relative phylum abundance revealed differences in the duodenal microbiota between FDN-positive and FDN-negative control samples, indicating intestinal dysbiosis. Bacteriological analysis of FDN lesions further identified Escherichia coli as a commonly present Gram-negative bacterium with potential pathogenic virulence genes associated with intestinal disease.
Article
Engineering, Biomedical
Tianjian Tong, Yijun Qi, Derrick Rollins, Debarpan Dhar, Cathy L. Miller, Chenxu Yu, Qun Wang
Summary: In this study, hollowed nanocarriers with PLL coating and mammalian orthoreovirus cell attachment protein & sigma;1 functionalization were explored as functional oral drug delivery vehicles. The fully functionalized ODDV system demonstrated significantly higher transport efficiency and protection of payloads, showing great potential as effective oral delivery vehicles for drugs and vaccines.
BIOACTIVE MATERIALS
(2023)
Article
Food Science & Technology
Phillip Brown, Yi Chen, Robin Siletzky, Cameron Parsons, Lee-Ann Jaykus, Joseph Eifert, Elliot Ryser, Catherine M. Logue, Christina Stam, Eric Brown, Sophia Kathariou
Summary: Listeria monocytogenes, a Gram-positive foodborne pathogen, has the ability to persist in food processing plants and contaminate ready-to-eat foods. A study investigating 54 L. monocytogenes strains from four turkey processing plants in the US found that allelic profiles across the whole genome can be specific to individual processing plants. Whole genome sequencing data has the potential to identify facility-specific signatures for tracking strain movement and environmental surveillance in food processing facilities.
FRONTIERS IN SUSTAINABLE FOOD SYSTEMS
(2021)
