Article
Surgery
Siv Fonnes, Tilde Rasmussen, Amanda Brunchmann, Barbara Juliane Holzknecht, Jacob Rosenberg
Summary: This systematic review and meta-analysis aimed to characterize the suspected or confirmed abdominal diseases and surgeries associated with Yersinia infection. The results showed that approximately half of the patients with mesenteric lymphadenitis and terminal ileitis were serologically positive for Yersinia spp. Infections, which may lead to unnecessary surgeries for suspected appendicitis.
JOURNAL OF SURGICAL RESEARCH
(2022)
Article
Gastroenterology & Hepatology
Alyssa C. Fasciano, Gaya S. Dasanayake, Mary K. Estes, Nicholas C. Zachos, David T. Breault, Ralph R. Isberg, Shumin Tan, Joan Mecsas
Summary: Researchers studied the interaction between the enteric pathogen Yersinia pseudotuberculosis (Yptb) and M cells using human ileal enteroid-derived monolayers. They found that the transcytosis of Yptb through M cells was influenced by its expression levels of T3SS, and that Yops impede the function of M cells, allowing early infectious stage Yptb to more effectively penetrate M cells.
Article
Cell Biology
Agata Lampart, Katarzyna Dominika Sluzalska, Aleksandra Czyrek, Aleksandra Szerszen, Jacek Otlewski, Antoni Wiedlocha, Malgorzata Zakrzewska
Summary: The nuclear localization sequence (NLS) in fibroblast growth factor 1 (FGF1) is not required for its intracellular anti-apoptotic activity in differentiated cells, suggesting that the mechanism of stress response differs according to the level of cell differentiation.
Article
Biochemistry & Molecular Biology
Catherine Amaya, Christopher J. F. Cameron, Swapnil C. Devarkar, Sebastian J. H. Seager, Mark B. Gerstein, Yong Xiong, Christian Schlieker
Summary: The research identified NOMO as a protein with unknown function that plays a crucial role in maintaining ER structure. Overexpression or depletion of NOMO can lead to abnormal ER morphology and impact the process of autophagy.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Shin-ichi Muroi, Yoichiro Isohama
Summary: This study showed that the C-terminal domain of AQP5 is crucial for the localization of AQP5 on the plasma membrane, with Leu(262) identified as a key amino acid. Mutations in the C-terminal domain resulted in AQP5 being localized in the autophagosome or lysosome, leading to decreased protein stability.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Ann-Katrin Kieninger, Piotr Tokarz, Ana Janovic, Martin Pilhofer, Gregor L. L. Weiss, Iris Maldener
Summary: This study identifies and characterizes the protein SepN as a new component of septal junctions in filamentous cyanobacterium Nostoc. It demonstrates the importance of controlling molecular diffusion between cells for the survival of multicellular organisms.
NATURE COMMUNICATIONS
(2022)
Article
Cell Biology
Nicola L. Stevenson, Dylan J. M. Bergen, Yinhui Lu, M. Esther Prada-Sanchez, Karl E. Kadler, Chrissy L. Hammond, David J. Stephens
Summary: Knockout of the golgin giantin leads to skeletal and craniofacial defects due to changes in glycosylation and extracellular matrix deposition. Our study reveals a conserved role for giantin in collagen biosynthesis and extracellular matrix assembly, as well as a giantin-dependent pathway for intracellular procollagen processing.
JOURNAL OF CELL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
William M. Moore, Candace Chan, Toshiki Ishikawa, Emilie A. Rennie, Heidi M-L Wipf, Veronica Benites, Maki Kawai-Yamada, Jenny C. Mortimer, Henrik Scheller
Summary: This study identified a sphingolipid glycosyltransferase GINT1 highly expressed in root nodules and roots colonized by arbuscular mycorrhizal fungi, demonstrating its role in synthesizing specific glycosyl inositol phosphoryl ceramides in plants. The results show that local reprogramming of glycosylation by MtGINT1 is essential for the persistence of endosymbionts within plant cells.
Article
Multidisciplinary Sciences
Lea Reuter, Tanja Schmidt, Prabha Manishankar, Christian Throm, Jutta Keicher, Andrea Bock, Irina Droste-Borel, Claudia Oecking
Summary: NPH3 plays a crucial role in auxin-dependent plant phototropism, with blue light triggering its dissociation from the plasma membrane through phosphorylation and interaction with 14-3-3 proteins. In darkness, NPH3 binds polyacidic phospholipids for membrane association. The dynamic change in NPH3 localization mediated by 14-3-3 is essential for auxin-dependent phototropism.
NATURE COMMUNICATIONS
(2021)
Article
Multidisciplinary Sciences
Sean L. Beckwith, Emily J. Nomberg, Abigail C. Newman, Jeannette V. Taylor, Ricardo C. Guerrero-Ferreira, David J. Garfinkel
Summary: A study found an intrinsically disordered N-terminal prion-like domain (PrLD) within the Gag protein of Ty1 retrotransposons, which is required for retrotransposition. Deleting the PrLD leads to defects in virus-like particle (VLP) assembly and retrotransposition. Chimeras of Ty1 Gag with PrLD replaced by other sequences display different retrotransposition phenotypes. This study provides a genetically tractable in vivo platform for studying PrLDs and encourages further research on the prevalence of PrLDs in other mobile elements.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Cell Biology
Sara Bilekova, Balma Garcia-Colomer, Alberto Cebrian-Serrano, Silvia Schirge, Karsten Krey, Michael Sterr, Thomas Kurth, Stefanie M. Hauck, Heiko Lickert
Summary: Spermatogenesis is a crucial biological process that produces functional sperm for successful reproduction. The acrosome, a lysosome-related organelle in the sperm head, is essential for egg-sperm recognition and fusion. The role of the insulin inhibitory receptor (inceptor) in acrosome formation was investigated, and its absence resulted in acrosomal malformation and male infertility.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Lise C. Noack, Vincent Bayle, Laia Armengot, Frederique Rozier, Adiilah Mamode-Cassim, Floris D. Stevens, Marie-Cecile Caillaud, Teun Munnik, Sebastien Mongrand, Roman Pleskot, Yvon Jaillais
Summary: Phosphoinositides, specifically phosphatidylinositol 4-phosphate (PI4P), play a crucial role in membrane identity acquisition, particularly at the plant plasma membrane. PI4K alpha 1 is anchored to the plasma membrane through a complex involving proteins from various families, and is essential for pollen, embryonic, and post-embryonic development. The immobilization of the PI4K alpha 1 complex in plasma membrane nanodomains is critical for its function, highlighting the importance of lipid kinases in plasma membrane nanopatterning.
Article
Cell Biology
Fei Sun, Fa-Xi Wang, He Zhu, Tian-Tian Yue, Chun-Liang Yang, Jia-Hui Luo, Xi Luo, Hai-Feng Zhou, Shan-Jie Rong, Wan-Ying Lu, Qing Zhou, Ping Yang, Fei Xiong, Yan-Jun Liu, Tong Yan, Yun-Fei Liao, Shu Zhang, Cong-Yi Wang
Summary: Our study found that UBC9-mediated protein SUMOylation plays a crucial role in peripheral CD4 T-cell proliferation, but has no noticeable impact on T-cell polarization. We also discovered that PDPK1 is a novel SUMOylation substrate and its decreased SUMOylation leads to impaired cell proliferation.
CELL DEATH & DISEASE
(2022)
Article
Biology
Wenyu Yang, Rui Tian, Yong Zhu, Peijie Huang, Xinrun Ma, Xiaoxiao Meng, Wentao Dai, Yiming Tao, Daonan Chen, Jiaxiang Zhang, Jian Lu, Hui Xie, Xiangdong Jian, Zhengfeng Yang, Ruilan Wang
Summary: Paraquat is a herbicide that induces epithelial-mesenchymal transition in pulmonary fibrosis. It acts as an agonist of STIM1, leading to extracellular calcium entry and increasing intracellular calcium levels, promoting the transition of alveolar cells.
COMMUNICATIONS BIOLOGY
(2022)
Article
Neurosciences
Xin-Shen Wu, Shobana Subramanian, Yalan Zhang, Bo Shi, Jessica Xia, Tiansheng Li, Xiaoli Guo, Lynda El-Hassar, Klara Szigeti-Buck, Jorge Henao-Mejia, Richard A. Flavell, Tamas L. Horvath, Elizabeth A. Jonas, Leonard K. Kaczmarek, Ling-Gang Wu
Summary: Potassium channels play a crucial role in endocytosis and vesicle mobilization by organizing the F-actin cytoskeleton, in addition to their traditional role in ion conductance. Mutations in potassium channels that impair these non-conducting functions may contribute to various neurological disorders.