Journal
INFECTION AND IMMUNITY
Volume 76, Issue 6, Pages 2551-2559Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/IAI.00338-08
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Funding
- NIAID NIH HHS [R01AI47841, K02AI054908, R01 AI047841, K02 AI054908] Funding Source: Medline
- NIDCR NIH HHS [R01DE18138, R01 DE018138] Funding Source: Medline
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Streptococcus sanguinis is one of the pioneers in the bacterial colonization of teeth and is one of the most abundant species in the oral biofilm called dental plaque. S. sanguinis is also the most common viridans group streptococcal species implicated in infective endocarditis. To investigate the association of biofilm and endocarditis, we established a biofilm assay and examined biofilm formation with a signature-tagged mutagenesis library of S. sanguinis. Four genes that have not previously been associated with biofilm formation in any other bacterium, purB, purL, thrB, and pyrE, were putatively identified as contributing to in vitro biofilm formation in S. sanguinis. By examining 800 mutants for attenuation in the rabbit endocarditis model and for reduction in biofilm formation in vitro, we found some mutants that were both biofilm defective and attenuated for endocarditis. However, we also identified mutants with only reduced biofilm formation or with only attenuation in the endocarditis model. This result indicates that the ability to form biofilms in vitro is not associated with endocarditis virulence in vivo in S. sanguinis.
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