Article
Immunology
Wanwu Li, Lingyan Jiang, Xiaoqian Liu, Rui Guo, Shuai Ma, Jingting Wang, Shuangshuang Ma, Shujie Li, Huiying Li
Summary: YhjC is identified as a necessary transcriptional regulator that positively regulates Shigella virulence by activating virF transcription. Deletion of YhjC results in decreased pathogenicity of Shigella, as well as downregulation of virF and other VirF-dependent genes. Additionally, YhjC functions as a global regulatory factor affecting the expression of multiple genes in the large virulence plasmid.
Article
Multidisciplinary Sciences
Oscar A. Campos, Narsis Attar, Chen Cheng, Maria Vogelauer, Nathan Mallipeddi, Stefan Schmollinger, Nedas Matulionis, Heather R. Christofk, Sabeeha S. Merchant, Siavash K. Kurdistani
Summary: This study reveals that histone H3-mediated Cu1+ toxicity is a major determinant of cellular functional pool of Fe-S clusters. Inadequate Fe-S cluster supply causes severe metabolic and growth defects. Decreasing Cu1+ abundance restores Fe-S cluster-dependent metabolism and growth.
Article
Infectious Diseases
Iqbal Nisa, Muhammad Qasim, Arnold Driessen, Jeroen Nijland, Fazal Adnan, Malik Nawaz Shuja, Hazir Rahman
Summary: This study investigated the distribution of virulence factors among different serotypes of S. flexneri in Pakistan, revealing that serotype 2b is the most virulent strain with a complete set of five virulence genes, which is associated with high disease severity.
INFECTION GENETICS AND EVOLUTION
(2021)
Review
Microbiology
Ahtesham Ahmad Shad, Wajahat Ahmed Shad
Summary: S. sonnei is an emerging pathogen globally, with increasing prevalence and significant role in occupying ecological niches of other gut pathogens. Recent studies suggest that improved sanitation has reduced cross-immunization with other strains, while enhancing its competitive advantage through specific secretion systems.
ARCHIVES OF MICROBIOLOGY
(2021)
Article
Medicine, General & Internal
Quanping Ma, Chengbao Zhu, Mingxiao Yao, Guangying Yuan, Yuguo Sun
Summary: This study examined the correlation between sulfamethoxazole-trimethoprim resistance in Shigella flexneri and antibiotic resistance genes sul1 and sul2. The sul1 and sul2 genes were found to be associated with the resistance of S. flexneri isolates to sulfamethoxazole-trimethoprim in this research.
Review
Plant Sciences
Bing Yang, Chenyun Xu, Yuting Cheng, Ting Jia, Xueyun Hu
Summary: Iron-sulfur (Fe-S) clusters are ancient protein cofactors that exist ubiquitously in organisms and play important roles in various life processes. Plastids, semi-autonomous organelles believed to originate from cyanobacterial endosymbionts, have a Fe-S cluster biosynthesis and delivery pathway similar to cyanobacteria. Fe-S clusters are crucial for the normal functioning of downstream Fe-S proteins in plastids. Recent research has made significant progress in understanding this pathway and this review summarizes these findings as well as the remaining scientific challenges.
PLANT CELL REPORTS
(2023)
Article
Biochemistry & Molecular Biology
Jorge Diaz-Rullo, Jose Eduardo Gonzalez-Pastor
Summary: In this study, we developed a novel bioinformatic strategy to predict Q-genes and found that these genes are widely enriched in cellular functions, especially in biofilm formation and virulence in bacteria, particularly in human pathogens. Experimental validation showed that altering the level of tRNA Q-modification significantly affected these physiological processes in different model bacteria, representing the first report of a general mechanism controlling biofilm formation and virulence in Gram-positive and Gram-negative bacteria. Additionally, we propose that changes in Q availability in a microbiome would impact its functionality. These findings open new avenues for the control of bacterial infections and biofilm formation by inhibiting tRNA Q-modification.
NUCLEIC ACIDS RESEARCH
(2023)
Article
Microbiology
Nicholas L. Xerri, Shelley M. Payne
Summary: The study revealed that the conditioned growth medium from the commensal bacterium Bacteroides thetaiotaomicron represses the invasion of Shigella flexneri. This repression is attributed to the presence of B. thetaiotaomicron outer membrane vesicles, indicating a novel role of bacterial outer membrane vesicles in modulating pathogenicity.
Article
Biochemistry & Molecular Biology
Jing Du, Zhaoyang Huang, Yanchun Li, Xueying Ren, Chaoting Zhou, Ruolan Liu, Ping Zhang, Guojie Lei, Jianxin Lyu, Jianghui Li, Guoqiang Tan
Summary: Copper is a necessary mineral nutrient but excess copper is cytotoxic. Wilson's disease is an autosomal recessive hereditary disease characterized by pathological copper accumulation in organs. The molecular mechanism of Wilson's disease is not fully understood and further research is needed to develop therapeutic strategies.
FREE RADICAL BIOLOGY AND MEDICINE
(2023)
Article
Biology
Seiya Watanabe, Yohsuke Murase, Yasunori Watanabe, Yasuhiro Sakurai, Kunihiko Tajima
Summary: Seiya Watanabe et al. report the crystal structures of two distinct members of the Aconitase X subfamily, which contain [Fe-S] clusters different from other aconitases. This study provides insight into the molecular evolution of the aconitase superfamily.
COMMUNICATIONS BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Ines Abatedaga, Barbara Perez Mora, Marisel Tuttobene, Gabriela Muller, Daiana Biancotti, Claudio D. Borsarelli, Lorena Valle, Maria A. Mussi
Summary: This study characterized the BLUF photoreceptors in A. nosocomialis and found that they have idiosyncratic characteristics compared to the previously characterized A. baumannii BlsA, including temperature-dependency and expression patterns. The results contribute to broadening our knowledge of the BLUF family.
Article
Multidisciplinary Sciences
Suppachai Srisantitham, Edward D. Badding, Daniel L. M. Suess
Summary: This study successfully incorporated Fe-57 into specific sites of the nitrogenase cofactor L-cluster, providing crucial insights into its electronic structure and biosynthesis. It introduces postbiosynthetic modification as a promising strategy for further studies on nitrogenase cofactors.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biochemistry & Molecular Biology
Isabelle Fix, Lorenz Heidinger, Thorsten Friedrich, Gunhild Layer
Summary: In archaea and sulfate-reducing bacteria, heme is synthesized via the siroheme-dependent pathway. The Radical SAM enzyme AhbD catalyzes the last step of this pathway, converting iron-coproporphyrin III into heme. Amino acid sequence analysis and structural modeling suggested that some AhbD proteins may contain two auxiliary iron-sulfur clusters. Study on AhbD from Methanosarcina barkeri confirmed the presence of two auxiliary [4Fe-4S] clusters, and the clusters likely participate in the heme synthase activity.
Article
Gastroenterology & Hepatology
Rimi Chowdhury, Paulina D. Pavinski Bitar, Katherine E. Bell, Craig Altier
Summary: Shigella flexneri can utilize fatty acids commonly found in the colon to suppress its virulence, increasing its growth rate and invasion ability. This study reveals how S. flexneri employs intestinal compounds as signals to enhance its pathogenicity.
Article
Microbiology
Xingju Song, Xu Yang, Zhu Ying, Kaijian Wu, Jing Liu, Qun Liu
Summary: Iron-sulfur [Fe-S] clusters are ancient and important prosthetic groups that play key roles in various metabolic processes. In this study, we investigated the role of mitochondrial glutaredoxin 5 (NcGRX5) in Neospora caninum, a parasite belonging to the phylum Apicomplexa. We found that deficiency of NcGRX5 led to abnormal mitochondrial structure and significantly reduced parasite proliferation and virulence. Comparative proteomics and energy metabolomics revealed that disruption of NcGRX5 downregulated expression of mitochondrial electron transport chain and tricarboxylic acid cycle proteins, affecting corresponding metabolic fluxes. We also identified proteins interacting with NcGRX5, including iron-sulfur cluster synthesis proteins ISCS and ISCU1. Our findings highlight the importance of NcGRX5 in parasite growth and mitochondrial energy metabolism through mediating iron-sulfur cluster biosynthesis.
MICROBIOLOGY SPECTRUM
(2023)
Article
Biotechnology & Applied Microbiology
Gili Hrusa, William Farmer, Brian L. Weiss, Taylor Applebaum, Jose Santinni Roma, Lauren Szeto, Serap Aksoy, Laura J. Runyen-Janecky
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2015)
Article
Microbiology
Haig A. Eskandarian, Pascal D. Odermatt, Joelle X. Y. Ven, Melanie T. M. Hannebelle, Adrian P. Nievergelt, Neeraj Dhar, John D. McKinney, Georg E. Fantner
NATURE MICROBIOLOGY
(2017)
Article
Biotechnology & Applied Microbiology
Caitlin L. Smith, Brian L. Weiss, Serap Aksoy, Laura J. Runyen-Janecky
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2013)
Article
Microbiology
Crystal Richardson, Malcolm Hill, Carolyn Marks, Laura Runyen-Janecky, April Hill
FEMS MICROBIOLOGY ECOLOGY
(2012)
Article
Microbiology
Chelsea D. Waddell, Thomas J. Walter, Sophia A. Pacheco, Georgiana E. Purdy, Laura J. Runyen-Janecky
JOURNAL OF BACTERIOLOGY
(2014)
Article
Microbiology
Aaron Daugherty, Akamol Eddie Suvarnapunya, Laura Runyen-Janecky
MICROBIOLOGICAL RESEARCH
(2012)
Article
Multidisciplinary Sciences
Haig A. Eskandarian, Francis Impens, Marie-Anne Nahori, Guillaume Soubigou, Jean-Yves Coppee, Pascale Cossart, Melanie A. Hamon
Review
Immunology
Laura J. Runyen-Janecky
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2013)
Article
Biology
Catherine Baranowski, Michael A. Welsh, Lok-To Sham, Haig A. Eskandarian, Hoong C. Lim, Karen J. Kieser, Jeffrey C. Wagner, John D. McKinney, Georg E. Fantner, Thomas R. Loerger, Suzanne Walker, Thomas G. Bernhardt, Eric J. Rubin, E. Hesper Rego
Article
Biochemistry & Molecular Biology
Michael Hohl, Sille Remm, Haig A. Eskandarian, Michael Dal Molin, Fabian M. Arnold, Lea M. Huerlimann, Andri Kruegel, Georg E. Fantner, Peter Sander, Markus A. Seeger
MOLECULAR MICROBIOLOGY
(2019)
Article
Biochemistry & Molecular Biology
Zuzana Palcekova, Shiva K. Angala, Juan Manuel Belardinelli, Haig A. Eskandarian, Maju Joe, Richard Brunton, Christopher Rithner, Victoria Jones, Jerome Nigou, Todd L. Lowary, Martine Gilleron, Michael McNeil, Mary Jackson
JOURNAL OF BIOLOGICAL CHEMISTRY
(2019)
Article
Physics, Multidisciplinary
Pascal D. Odermatt, Melanie T. M. Hannebelle, Haig A. Eskandarian, Adrian P. Nievergelt, John D. McKinney, Georg E. Fantner
Article
Infectious Diseases
Jose Santinni Roma, Shaina D'Souza, Patrick J. Somers, Leah F. Cabo, Ruhan Farsin, Serap Aksoy, Laura J. Runyen-Janecky, Brian L. Weiss
PLOS NEGLECTED TROPICAL DISEASES
(2019)
Article
Multidisciplinary Sciences
Melanie T. M. Hannebelle, Joelle X. Y. Ven, Chiara Toniolo, Haig A. Eskandarian, Gaelle Vuaridel-Thurre, John D. McKinney, Georg E. Fantner
NATURE COMMUNICATIONS
(2020)
Article
Biochemistry & Molecular Biology
Adrian P. Nievergelt, Charlene Brillard, Haig A. Eskandarian, John D. McKinney, Georg E. Fantner
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2018)