Article
Multidisciplinary Sciences
Melissa J. Martin, Brendan W. Corey, Filomena Sannio, Lindsey R. Hall, Ulrike MacDonald, Brendan T. Jones, Emma G. Mills, Casey Harless, Jason Stam, Rosslyn Maybank, Yoon Kwak, Katharina Schaufler, Karsten Becker, Nils-Olaf Hubner, Stefania Cresti, Giacinta Tordini, Marcello Valassina, Maria Grazia Cusi, Jason W. Bennett, Thomas A. Russo, Patrick T. McGann, Francois Lebreton, Jean-Denis Docquier
Summary: This study conducted genomic analysis of NDM-producing carbapenem-resistant Klebsiella pneumoniae in Tuscany, Italy, finding that the isolates belonged to the high-risk clone ST-147 and shared a recent ancestor with clinical isolates from the Middle East. Additionally, it was discovered that these isolates carried a new plasmid, indicating mutations that confer resistance to multiple antibiotics.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Immunology
Xiaoyan Wu, Xiangchen Li, Junjie Yu, Mengli Shen, Chenliang Fan, Yewei Lu, Junshun Gao, Xiaosi Li, Hongsheng Li
Summary: This study investigates the first outbreak of OXA-232-producing carbapenem-resistant Klebsiella pneumoniae (CRKP) in the Second Affiliated Hospital of Jiaxing University. Whole-genome sequencing revealed that all 21 OXA-232-producing CRKP strains belonged to sequence type 15 (ST15) and shared similar resistance, virulence genes, and plasmid types, suggesting clonal transmission between the environment and patients. Integrated genomic and epidemiological analysis traced the outbreak to two clonal transmission clusters, including 14 and 2 patients.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Infectious Diseases
Xinyi Yang, Xu Liu, Weibin Li, Lin Shi, Yingchao Zeng, Haohai Xia, Qixian Huang, Jia Li, Xiaojie Li, Bo Hu, Lianping Yang
Summary: To investigate the impact of the COVID-19 pandemic on carbapenem-resistant organisms (CROs), the epidemiological characteristics and resistance changes of CROs were analyzed. The study found that the resistance rate of carbapenem-resistant Acinetobacter baumannii (CRAB) increased significantly after the COVID-19 outbreak, while the resistance rate of Klebsiella pneumoniae remained stable. Stratified analysis revealed that factors such as gender, age, sample type, and clinical department contributed to the resistance rate of Klebsiella pneumoniae. This highlights the importance of closely monitoring CROs, especially in high-risk populations and clinical departments, during the COVID-19 pandemic.
Article
Medical Laboratory Technology
Pengwen Ouyang, Bin Jiang, Na Peng, Juan Wang, Liang Cai, Yi Wu, Jianrong Ye, Yiping Chen, Hao Yuan, Chaochao Tan, Liming Tan, Liangyi Xie
Summary: The study analyzed the microbiological and clinical characteristics of carbapenem-resistant hypervirulent Klebsiella pneumoniae (CR-hvKP) that causes nosocomial infection. The main type of CRKP that caused nosocomial infection was found to be ST11 KPC-2-producing CR-hvKP, and clonal spread has occurred.
JOURNAL OF CLINICAL LABORATORY ANALYSIS
(2022)
Article
Infectious Diseases
Sriram Kk, Tsegaye Sewunet, Walaiporn Wangchinda, Teerawit Tangkoskul, Visanu Thamlikitkul, Christian G. Giske, Fredrik Westerlund
Summary: In this study, 27 CR-KP isolates from 16 patients were analyzed using NGS and ODM, with most isolates belonging to ST16. The plasmid carrying the bla(NDM-1) gene was identified in the ST16 isolates, and the potential of using ODM of plasmids as a marker for bacterial spread was highlighted. Additionally, ODM was able to differentiate between plasmids carrying different resistance genes in the ST16 isolates.
Article
Genetics & Heredity
To Nguyen Thi Nguyen, Phuong Luong Nha Nguyen, Ngan Thi Quynh Le, Lan Phu Huong Nguyen, Thuy Bich Duong, Nghia Dang Trung Ho, Quynh Pham Nhu Nguyen, Trung Duc Pham, Anh Tuan Tran, Hao Chung The, Hien Huu Nguyen, Chau Van Vinh Nguyen, Guy E. Thwaites, Maia A. Rabaa, Duy Thanh Pham
Summary: The emergence of carbapenem resistance in Klebsiella pneumoniae is a major global public health concern, with two high-mortality nosocomial outbreaks occurring in a hospital in Vietnam. Genome sequencing and phylogenetic analysis identified two distinct lineages of the international sequence type 16 clone causing the outbreaks, with extensive drug resistance and potential nosocomial spread from high-touch surfaces. The study highlights the importance of coordinated efforts between clinical and molecular microbiologists and infection control teams to effectively identify and contain nosocomial outbreaks.
MICROBIAL GENOMICS
(2021)
Article
Biology
Natalia C. Rosas, Jonathan Wilksch, Jake Barber, Jiahui Li, Yanan Wang, Zhewei Sun, Andrea Rocker, Chaille T. Webb, Laura Perlaza-Jimenez, Christopher J. Stubenrauch, Vijaykrishna Dhanasekaran, Jiangning Song, George Taiaroa, Mark Davies, Richard A. Strugnell, Qiyu Bao, Tieli Zhou, Michael J. McDonald, Trevor Lithgow
Summary: This study reconstructed the genetic and evolutionary mechanisms of carbapenem resistance in a clinical isolate of Klebsiella quasipneumoniae. It found that multiple genetic loci are necessary for acquiring carbapenem resistance, and the selection of one gene by an antibiotic can facilitate the mutation of another gene, further enhancing the evolution of carbapenem resistance.
Article
Infectious Diseases
Gilberto G. Gaspar, Gustavo Tamasco, Nathalia Abichabki, Ana Flavia T. Scaranello, Maria Auxiliadora-Martins, Renata Pocente, Leonardo N. Andrade, Maria-Eugenia Guazzaroni, Rafael Silva-Rocha, Valdes R. Bollela
Summary: We studied an outbreak of polymyxin B (PB)- and carbapenem-resistant Klebsiella pneumoniae during the COVID-19 pandemic and found that all isolates showed resistance to multiple drugs, with one particular subtype spreading in the ICU. This outbreak was related to the collapse of the local healthcare system and resulted in high mortality rates.
Article
Multidisciplinary Sciences
Marisa Zenaide Ribeiro Gomes, Elisangela Martins J. de Lima, Caio Augusto Martins Aires, Polyana Silva Pereira, Juwon Yim, Fernando Henrique Silva, Caio Augusto Santos Rodrigues, Thamirys Rachel Tavares e Oliveira, Priscila Pinho da Silva, Cristiane Monteiro Eller, Claudio Marcos Rocha de Souza, Michael Rybak, Rodolpho Mattos Albano, Antonio Basilio de Miranda, Edson Machado, Marcos Catanho
Summary: We report a PCR-Kp outbreak causing untreatable infections descriptively correlated with bacterial genomes. A target phenotypic profile was detected in 10% of the tested patients, who had epidemiological links and were involved in intractable infections and death.
SCIENTIFIC REPORTS
(2023)
Article
Microbiology
Andreas G. Tofarides, Panagiotis Dimitriou, Georgios K. Nikolopoulos, Dimitrios Rogkas, Christina Flourou, Elina Khattab, Diamanto Kasapi, Chara Azina, Eirini Christaki
Summary: Klebsiella pneumoniae is a leading cause of hospital-acquired infections, and resistant strains are becoming increasingly common. This study found that recent antimicrobial use and heart disease are associated with bloodstream infections caused by ESBL-producing Klebsiella pneumoniae.
Article
Infectious Diseases
Marco Coppi, Alberto Antonelli, Claudia Niccolail, Andrea Bartolinil, Laura Bartolini, Maddalena Grazzini, Elisabetta Mantengoli, Alberto Faresek, Filippo Pierallis, Maria Teresa Mechi, Vincenzo Di Pilato, Tommaso Giani, Gian Maria Rossolini
Summary: This study reports a nosocomial outbreak caused by a cefiderocol-resistant NDM-1-producing Klebsiella pneumoniae (NDM-Kp) strain in a large tertiary care hospital in Florence, Italy. The outbreak was sustained by clonal expansion of a mutant with an inactivated cirA gene, which exhibited high-level resistance to FDC independently of exposure to the antibiotic.
Article
Microbiology
Feiyang Zhang, Qin Li, Jiawei Bai, Manlin Ding, Xiangjin Yan, Guangxi Wang, Baoli Zhu, Yingshun Zhou
Summary: This study revealed that 8.39% of Klebsiella pneumoniae strains were amikacin-heteroresistant, with potential association with increased expression of aminoglycoside resistance genes. The majority of heteroresistant phenotypes were unstable, with partial or full reversion of minimal inhibitory concentrations to the level of susceptibility.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Microbiology
Junying Zhu, Xuemei Jiang, Lina Zhao, Min Li
Summary: An outbreak caused by carbapenem-resistant hypervirulent Klebsiella pneumoniae (CR-hvKP) ST859-K19 isolates occurred in a teaching hospital in China. The isolates showed high-level resistance to commonly used antibiotics and had almost identical resistance and virulence gene profiles, indicating possible clone transmission. This study provides important insights into the molecular and genomic characteristics of ST859 CR-hvKP isolates and highlights the need for active surveillance to detect the spread of CR-hvKP in healthcare settings.
Article
Infectious Diseases
Mykhailo Savin, Gabriele Bierbaum, Nico T. Mutters, Ricarda Maria Schmithausen, Judith Kreyenschmidt, Isidro Garcia-Menino, Silvia Schmoger, Annemarie Kaesbohrer, Jens Andre Hammerl
Summary: This study characterizes carbapenem-resistant Klebsiella spp. in wastewater and surface water in Germany. The isolates showed resistance to multiple antibiotics and a high diversity of antibiotic-resistance genes. Virulence factors were also present in most isolates. The findings suggest the possible dissemination of resistant bacteria in the environment and the risk of colonization and infection in humans, livestock, and wildlife.
Article
Microbiology
Yujiao Wang, Bo Zhu, Min Liu, Xiutao Dong, Jianping Ma, Xiaofeng Li, Fang Cheng, Jianzhuang Guo, Sumei Lu, Furong Wan, Yingying Hao, Wanshan Ma, Mingju Hao, Liang Chen
Summary: The study reported three Klebsiella species strains carrying TmexCD2-ToprJ2, indicating the spread of this novel plasmid-mediated tigecycline resistance mechanism to different Klebsiella species. The co-occurrence of bla(NDM-1) and TmexCD2-ToprJ2 in the same plasmid is of particular public health concern.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Infectious Diseases
S. Hagel, K. Ludewig, M. W. Pletz, J. Frosinski, A. Moeser, M. Wolkewitz, P. Gastmeier, S. Harbarth, F. M. Brunkhorst, M. Kesselmeier, A. Scherag
CLINICAL MICROBIOLOGY AND INFECTION
(2019)
Article
Public, Environmental & Occupational Health
Seven Johannes Sam Aghdassi, Christin Schroeder, Desiree Gruhl, Petra Gastmeier, Florian Salm
ANTIMICROBIAL RESISTANCE AND INFECTION CONTROL
(2019)
Article
Medicine, General & Internal
Cristina Diaz-Agero Perez, Nieves Lopez-Fresnena, Angela L. Rincon Carlavilla, Marta Hernandez Garcia, Patricia Ruiz-Garbajosa, Jesus Maria Aranaz-Andres, Friederike Maechler, Petra Gastmeier, Marc J. M. Bonten, Rafael Canton
Article
Microbiology
Christian Schweizer, Peter Sischoff, Jennifer Bender, Axel Kola, Petra Gastmeier, Manfred Hummel, Frank-Rainer Klefisch, Felix Schoenrath, Andre Fruehauf, Yvonne Pfeifer
FRONTIERS IN MICROBIOLOGY
(2019)
Article
Public, Environmental & Occupational Health
Walter Zingg, Julie Storr, Benjamin J. Park, Raheelah Ahmad, Carolyn Tarrant, Enrique Castro-Sanchez, Sara Tomczyk, Claire Kilpatrick, Benedetta Allegranzi, Denise Cardo, Didier Pittet, Mohamed Abbas, Raheelah Ahmad, Benedetta Allegranzi, Antoine Andremont, Mike Bell, Michael Borg, Denise Cardo, Yehuda Carmeli, Enrique Castro-Sanchez, John Conly, Philippe Eggimann, Petra Gastmeier, M. Lindsay Grayson, Stephan Harbarth, Marcela Hernandez, Loreen Herwaldt, Alison Holmes, John A. Jernigan, Claire Kilpatrick, Amy Kolwaite, Karl-Heinz Krause, Elaine Larson, Sarah Masson-Roy, Shaheen Mehtar, Marc Mendelson, Ling Moi Lin, Andreea Moldovan, Dominique Monnet, Babacar Ndoye, Peter Nthumba, Folasade Ogunsola, Ben Park, Eli Perencevich, Didier Pittet, Matthew Samore, Wing Hong Seto, Arjun Srinivasan, Julie Storr, Evelina Tacconelli, Carolyn Tarrant, Sara Tomczyk, Maha Talaat, Maria Virginia Villegas, Andreas Voss, Tim Walsh, Andreas Widmer, Walter Zingg
ANTIMICROBIAL RESISTANCE AND INFECTION CONTROL
(2019)
Article
Public, Environmental & Occupational Health
Seven Johannes Sam Aghdassi, Christin Schroeder, Petra Gastmeier
ANTIMICROBIAL RESISTANCE AND INFECTION CONTROL
(2019)
Article
Medicine, General & Internal
Seven Johannes Sam Aghdassi, Frank Schwab, Peter Hoffmann, Petra Gastmeier
DEUTSCHES ARZTEBLATT INTERNATIONAL
(2019)
Article
Infectious Diseases
Benedikt Zacher, Sebastian Haller, Niklas Willrich, Jan Walter, Muna Abu Sin, Alessandro Cassini, Diamantis Plachouras, Carl Suetens, Michael Behnke, Petra Gastmeier, Lothar H. Wieler, Tim Eckmanns
Article
Infectious Diseases
Seven Johannes Sam Aghdassi, Frank Schwab, Sonja Hansen, Luis Alberto Pena Diaz, Michael Behnke, Petra Gastmeier, Tobias Siegfried Kramer
Article
Public, Environmental & Occupational Health
Lynn Meurs, Felix S. Lempp, Norman Lippmann, Henning Trawinski, Arne C. Rodloff, Matthias Eckardt, Anja Klingeberg, Tim Eckmanns, Jan Walter, Christoph Luebbert, Muna Abu Sin, Esther-Maria Antao, Michael Behnke, Jutta Bleidorn, Evgeniya Boklage, Christina Forstner, Petra Gastmeier, Jochen Gensichen, Alexander Gropmann, Stefan Hagel, Regina Hanke, Wolfgang Hanke, Lukas Klimmek, Ulrich Kraft, Tobias Kramer, Markus Lehmkuhl, Norman Ludwig, Antina Luebke-Becker, Oliwia Makarewicz, Anne Moeser, Inga Petruschke, Mathias W. Pletz, Cornelius Remschmidt, Florian Salm, Katja Schmuecker, Sandra Schneider, Christin Schroeder, Frank Schwab, Joachim Trebbe, Szilvia Vincze, Horst C. Vollmar, Sebastian Weis, Wibke Wetzker, Lothar H. Wieler
TRAVEL MEDICINE AND INFECTIOUS DISEASE
(2020)
Article
Infectious Diseases
L. A. Denkel, F. Maechler, F. Schwab, A. Kola, A. Weber, P. Gastmeier, F. Paeffflin, S. Weber, G. Werner, Y. Pfeifer, M. Pietsch, R. Leistner
CLINICAL MICROBIOLOGY AND INFECTION
(2020)
Article
Infectious Diseases
Jakob J. Malin, Christoph D. Spinner, Uwe Janssens, Tobias Welte, Steffen Weber-Carstens, Gereon Schaelte, Petra Gastmeier, Florian Langer, Martin Wepler, Michael Westhoff, Michael Pfeifer, Klaus F. Rabe, Florian Hoffmann, Bernd W. Bottiger, Julia Weinmann-Menke, Alexander Kersten, Peter Berlit, Marcin Krawczyk, Wiebke Nehls, Falk Fichtner, Sven Laudi, Miriam Stegemann, Nicole Skoetz, Monika Nothacker, Gernot Marx, Christian Karagiannidis, Stefan Kluge
Summary: This executive summary of a national living guideline provides rapid evidence-based recommendations on drug interventions for hospitalized patients with COVID-19, using systematic assessment and decision processes. 11 key recommendations for COVID-19 drug therapy are included in the guideline, with strong recommendations for corticosteroids and prophylactic anticoagulation as standard of care. The guidelines will be updated as new evidence emerges.
Correction
Infectious Diseases
Jakob J. Malin, Christoph D. Spinner, Uwe Janssens, Tobias Welte, Steffen Weber-Carstens, Gereon Schaelte, Petra Gastmeier, Florian Langer, Martin Wepler, Michael Westhoff, Michael Pfeifer, Klaus F. Rabe, Florian Hoffmann, Bernd W. Boettiger, Julia Weinmann-Menke, Alexander Kersten, Peter Berlit, Marcin Krawczyk, Wiebke Nehls, Falk Fichtner, Sven Laudi, Miriam Stegemann, Nicole Skoetz, Monika Nothacker, Gernot Marx, Christian Karagiannidis, Stefan Kluge
Article
Infectious Diseases
Peter Bischoff, Tobias Siegfried Kramer, Christin Schroeder, Michael Behnke, Frank Schwab, Christine Geffers, Petra Gastmeier, Seven Johannes Sam Aghdassi
Summary: This study investigated the correlation between age and surgical site infection (SSI) occurrence, and found that older age is associated with a higher risk of SSI.
Article
Infectious Diseases
Anna M. Rohde, Alexander Mischnik, Michael Behnke, Ariane Dinkelacker, Simone Eisenbeis, Jane Falgenhauer, Petra Gastmeier, Georg Haecker, Susanne Herold, Can Imirzalioglu, Nadja Kaeding, Evelyn Kramme, Silke Peter, Ellen Piepenbrock, Jan Rupp, Christian Schneider, Frank Schwab, Harald Seifert, Michaela Steib-Bauert, Evelina Tacconelli, Janina Trauth, Maria J. G. T. Vehreschild, Sarah Walker, Winfried Kern, Nathalie Jazmati, DZIF R NET Study Grp
Summary: This study analysed the influence of antibiotic consumption on healthcare-associated healthcare onset Clostridioides difficile infection (CDI) in a German university hospital setting. The findings showed that carbapenem (mostly meropenem) consumption was associated with an increased incidence density of HAHO-CDI, particularly in haematology-oncology wards. Other patient-specific factors and ward specialty were equally important in controlling HAHO-CDI.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2023)