Article
Biotechnology & Applied Microbiology
Lei Sun, Yingguo Bai, Xiu Zhang, Cheng Zhou, Jie Zhang, Xiaoyun Su, Huiying Luo, Bin Yao, Yuan Wang, Tao Tu
Summary: Three recombinant GAD genes from Bacillus strains were identified and successfully expressed heterologously in Escherichia coli. Using the engineered E. coli strain as a whole-cell biocatalyst, the biosynthesis of GABA from L-Glu was achieved, showing potential for industrial production of GABA.
MICROBIAL CELL FACTORIES
(2021)
Article
Agronomy
Xiao Liu, Hao Ma, Jing Liu, Donghe Liu, Chunlei Wang
Summary: Watercore is a physiological disorder in Rosaceae fruits that causes hypoxia stress and fruit decay. The key enzyme PpGAD2 plays an important role in GABA synthesis, and exogenous GABA can inhibit the content of ethanol, MDA, and H2O2. PpGAD2 is significantly induced in watercore fruit, and its activity is affected by PpWRKY53 and several CML genes.
Article
Chemistry, Applied
Yu Sun, Dayi Ji, Haile Ma, Xiumin Chen
Summary: This study aimed to uncover the mechanism of GABA enrichment in coffee leaves under ultrasound treatment. The results showed that ultrasound increased the activity of enzymes related to GABA synthesis and changed the microstructure of coffee leaves, accelerating the migration of glutamate into cells. Metabolomics analysis identified various metabolic pathways involved in GABA increase.
Article
Food Science & Technology
Zhou Chen, Run Wang, Yanyin Song, Aijin Ma, Siting Li, Yingmin Jia
Summary: Gamma-aminobutyric acid (GABA) is a potent bioactive compound with many anti-hypertensive and anti-depressant activities. A novel pyridoxal-5-phosphate monohydrate-dependent GAD (LcGAD10s) was cloned from Latilactobacillus curvatus and expressed in E. coli. LcGAD10s exhibited high stability and efficiency in converting GABA.
Article
Biotechnology & Applied Microbiology
Hyo Jung Lim, Dong-Hyun Jung, Eui-Sang Cho, Myung-Ji Seo
Summary: This study reports the first Gad from Lc.garvieae species and provides important enzymatic information related to GABA biosynthesis in the Lactococcus genus.
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
(2022)
Article
Multidisciplinary Sciences
Mohammad Reza Edalatian Dovom, Mohammad Bagher Habibi Najafi, Paria Rahnama Vosough, Neda Norouzi, Seyyed Javad Ebadi Nezhad, Baltasar Mayo
Summary: A total of 50 lactic acid bacteria isolates from Iranian traditional dairy products were screened for GABA production. Eight isolates showed strong GAD activity and two Lactococcus lactis strains (311 and 491) were the strongest GABA producers. Optimal conditions for GABA production by these strains were determined.
SCIENTIFIC REPORTS
(2023)
Article
Microbiology
Xinyi Gu, Jiancun Zhao, Rongling Zhang, Ruohan Yu, Tingting Guo, Jian Kong
Summary: Enterococcus avium is a common bacterium found in the intestines of humans and animals, capable of producing GABA. Research showed that the GAD system in E. avium strains is encoded by three gadB genes, with gadB3 playing a crucial role in GABA biosynthesis and acid tolerance. This insight provides a better understanding of how E. avium strains survive in acidic environments and maintain cell viability during transit through the stomach and gut.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Food Science & Technology
Su Jeong Park, Dong Hyun Kim, Hye Jee Kang, Minhye Shin, Soo-Yeon Yang, Jungwoo Yang, Young Hoon Jung
Summary: In this study, a synthetic medium was used to efficiently produce high titer of GABA using Lactobacillus plantarum. The optimized medium composition resulted in the highest GABA production compared to previous studies. The use of yeast extract as a secondary substrate source also contributed to achieving a yield exceeding the theoretical maximum.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2021)
Article
Neurosciences
Hideki Miwa, Ken Kobayashi, Shinobu Hirai, Mitsuhiko Yamada, Masahiko Watanabe, Haruo Okado, Yuchio Yanagawa
Summary: The study reveals the critical role of GABA in establishing connections between basket cells and Purkinje cells in the cerebellum, and its association with motor coordination in mice.
Article
Plant Sciences
Qinling Wei, Keqin Xie, Hongfei Wang, Xingfeng Shao, Yingying Wei, Yi Chen, Shu Jiang, Mengze Cao, Jisuan Chen, Feng Xu
Summary: The study investigated the effect of fructose on the content and metabolic pathway of gamma-aminobutyric acid (GABA) in broccoli sprouts. The results showed that fructose treatment increased the fresh weight, GABA, and glutamate contents in sprouts and promoted the activity of glutamic acid decarboxylase (GAD) and the expressions of BoGAD1 and BoGAD2. Fructose treatment also inhibited the stem length of broccoli sprouts and enhanced abscisic acid (ABA) production. Additionally, fructose treatment stimulated the Ca2+, CaM contents, and BoCaM2 expression, as well as increased inositol trisphosphate (IP3) content and activated phosphatidylinositol-specific phospholipase C (PI-PLC) activity and BoPLC2 expression, contributing to Ca2+ influx into the cells.
Article
Biotechnology & Applied Microbiology
Sukanya Phuengjayaem, Auttaporn Booncharoen, Somboon Tanasupawat
Summary: This study screened and characterized 15 lactic acid bacteria isolates capable of producing GABA, with a focus on strains LSI2-1 and CAB1-7 isolated from pickled vegetables, which showed high GABA production levels. Additionally, the presence of the gadA gene in these strains indicates their potential for GABA production.
BIOTECHNOLOGY LETTERS
(2021)
Article
Agricultural Engineering
Qiaohong Chen, Yi Chen, Qiang Hu, Danxiang Han
Summary: The utilization of gibberellic acid-3, high carbon/nitrogen ratio, and salinity concentration can effectively enhance astaxanthin biosynthesis in Chromochloris zofingiensis under heterotrophic conditions. Metabolomics analysis revealed that enhanced glycolysis, pentose phosphate pathways, and tricarboxylic acid cycle led to astaxanthin accumulation. Increased fatty acids also contributed to astaxanthin esterification. The addition of appropriate concentrations of glycine and γ-aminobutyric acid promoted astaxanthin biosynthesis and biomass yield in C. zofingiensis.
BIORESOURCE TECHNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Wenli Liu, Huamin Li, Long Liu, Kanghee Ko, Incheol Kim
Summary: This study screened seven strains of LAB producing GABA from kimchi, with F109-MD3 showing the highest GABA-producing ability. The strains, especially F109-MD3, were able to produce GABA effectively even in high concentrations of MSG, and the GAD activity exhibited a broad pH range and higher optimal temperature.
JOURNAL OF APPLIED MICROBIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Lovely Mahawar, Aparna Pandey, Kesava Priyan Ramasamy, Sakshi Pandey, Sheo Mohan Prasad
Summary: This article provides an overview of the research progress on the synthesis and metabolic pathways of GABA in cyanobacteria, as well as the feasibility of enhancing GABA production and extraction methods by modifying external conditions and gene expression. In addition, it introduces the commercial applications of GABA in various industries.
JOURNAL OF APPLIED PHYCOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Fengmin Liu, Heng Zhang, Xiangjun Zhang, Xiaobo Wei, Huiyan Liu, Haitian Fang
Summary: Gamma-aminobutyric acid (GABA) is a water-soluble non-proteinogenic amino acid widely used in the food and pharmaceutical industries. Enzymatic conversion, catalyzed by glutamic acid decarboxylase (GAD), is an efficient method for GABA production. Directed mutation at specific sites, such as Gad B-T62S and Gad B-Q309A, can improve the catalytic properties and practical application of GAD. GABA production using recombinant strains of Escherichia coli showed promising results with high GABA concentrations and transformation rates.
ENGINEERING IN LIFE SCIENCES
(2023)
Article
Agriculture, Multidisciplinary
Min-A Song, Jae Sin Park, Ki Deok Kim, Yong Chull Jeun
PLANT PATHOLOGY JOURNAL
(2015)
Article
Agriculture, Multidisciplinary
Jeum Kyu Hong, Hye Ji Yang, Heesoo Jung, Dong June Yoon, Mee Kyung Sang, Yong-Chull Jeun
PLANT PATHOLOGY JOURNAL
(2015)
Article
Agronomy
Hye Sook Kim, Mee Kyung Sang, Yong-Chull Jeun, Byung Kook Hwang, Ki Deok Kim
Article
Agronomy
Yongjun An, Seogchan Kang, Ki-Deok Kim, Byung Kook Hwang, Yongchull Jeun
Article
Agronomy
Hye-Sook Kim, Mee Kyung Sang, Ho Won Jung, Yong-Chull Jeun, Inn-Shik Myung, Ki Deok Kim
Article
Microbiology
Yun Jung Ko, Ju Sung Kim, Ki Deok Kim, Yong Chull Jeun
JOURNAL OF MICROBIOLOGY
(2014)
Article
Microbiology
So Young Kang, Ki Deok Kim, Jeum Kyu Hong, He Nam Hyun, Yong Chull Jeun
JOURNAL OF MICROBIOLOGY
(2014)
Article
Plant Sciences
Mee Kyung Sang, Eui Nam Kim, Gyung Deok Han, Min Sun Kwack, Yong Chull Jeun, Ki Deok Kim
Article
Biochemistry & Molecular Biology
Dae Sung Kim, Yongchull Jeun, Byung Kook Hwang
PLANT MOLECULAR BIOLOGY
(2014)
Article
Agronomy
Eun Ju Ko, Yong Ho Shin, He Nam Hyun, Hyo Soon Song, Jeum Kyu Hong, Yong Chull Jeun
Article
Agriculture, Multidisciplinary
Su Jeung Kim, Eun Ju Ko, Jeum Kyu Hong, Yong Chull Jeun
PLANT PATHOLOGY JOURNAL
(2018)
Article
Agriculture, Multidisciplinary
Jin Young Park, Su Hyeon Kim, Na Hee Kim, Sang Woo Lee, Yong-Chull Jeun, Jeum Kyu Hong
PLANT PATHOLOGY JOURNAL
(2017)
Article
Plant Sciences
Y. C. Jeun, K. W. Kim, K. D. Kim, J. W. Hyun
JOURNAL OF PHYTOPATHOLOGY
(2007)
Article
Agriculture, Multidisciplinary
Hyo-Jeong Kim, Yong-Chull Jeun
PLANT PATHOLOGY JOURNAL
(2007)
Article
Agricultural Engineering
Jingmo Zhou, Shubin Wu, Ying Liu
Summary: Expenditure isolation of lignin and the understanding of its structural variations during different isolation processes are crucial for the high-value utilization of lignin. This study proposed a green deep eutectic solvent (DES) consisting of choline chloride, ethylene glycol, and 4-chlorobenzene sulfonic acid for rapid lignin separation from poplar. The structural variations of DES lignin and its relationship with alkali pre-extraction of hemicellulose were investigated. The results showed that alkali pretreatment enhanced the DES separation of lignin and promoted the cleavage of specific linkages, resulting in reduced molecular weight and thermal stability of isolated DES lignin. Moreover, DES lignin obtained from integrated utilization displayed higher purity and S/G ratio compared to lignin obtained from direct DES treatment.
INDUSTRIAL CROPS AND PRODUCTS
(2024)
Article
Agricultural Engineering
Yuping Li, Ziyan Xie, Yun Huang, Jiangyi Zeng, Chao Yang, Ling Yuan, Ying Wang, Yongqing Li
Summary: In this study, the characteristic flavonoids (CFs) of three licorice species were identified through integrated metabolic and transcriptomic analysis. The key regulators of CF biosynthesis were also identified. These findings provide valuable information for understanding the biosynthesis of CFs and future breeding of Glycyrrhiza varieties.
INDUSTRIAL CROPS AND PRODUCTS
(2024)
Article
Agricultural Engineering
Xianjie Pan, Zhaoyi Luo, Fanqi Liu, Quanqian Yi, Yanning Zeng
Summary: This study presents a novel strategy for fabricating fully bio-based epoxy vitrimers using peach gum and itaconic acid, which exhibit superior mechanical properties and thermostability. The materials were characterized and the self-healing and recycling capabilities were evaluated. The results demonstrate the potential of using these materials for sustainable industrial applications.
INDUSTRIAL CROPS AND PRODUCTS
(2024)
Article
Agricultural Engineering
Mohsen Sabzi-Nojadeh, Mohammad Pouresmaeil, Mina Amani, Mehdi Younessi-Hamzekhanlu, Filippo Maggi
Summary: This study shows that inoculating Summer savory plants with endophytic fungus Trichoderma harzianum can improve plant growth and increase salt tolerance. The fungus enhances growth, restores chlorophyll and antioxidant levels, reduces harmful substances, and increases proline and soluble sugar levels, thereby increasing crop productivity in saline conditions.
INDUSTRIAL CROPS AND PRODUCTS
(2024)
Article
Agricultural Engineering
Yu Liu, Lei Li, Aichen Zhao, Xiuming Song, Lulu Wei, Mengdi Fang, Chuchu Zheng, Xiaodong Zhu
Summary: This study investigates the impact of peppermint essential oil microcapsules on the unpleasant odor emissions from wax oil coated wood products. The results show that microcapsules can improve the time and stability of odor release.
INDUSTRIAL CROPS AND PRODUCTS
(2024)
Article
Agricultural Engineering
Mesut Budak, Mirac Kilic, Hikmet Guenal, Ismail Velik, Mesut Sirri
Summary: This study assessed land suitability for rapeseed production using topography, climate, and soil data by analytical hierarchy process (AHP) and the Mamdani Fuzzy Inference System (MFIS). The study area covered land in the Diyarbakir province of southeastern Turkiye. The results indicated the proportions of different land suitability classes and showed the compatibility of AHP and MFIS methods in assessing suitability.
INDUSTRIAL CROPS AND PRODUCTS
(2024)
Article
Agricultural Engineering
Rui Tian, Yue Liu, Chao Wang, Weikun Jiang, Srinivas Janaswamy, Guihua Yang, Xingxiang Ji, Gaojin Lyu
Summary: In this study, hollow and solid lignin nanoparticles were synthesized through self-assembly and their application in PVA nanocomposite films was investigated. The results showed that the presence of hollow lignin nanoparticles in the films resulted in a room-like network structure, while the solid lignin nanoparticles induced different structural changes. The inclusion of LNPs improved the mechanical properties of the films, and both types of LNPs exhibited good hydrophobicity.
INDUSTRIAL CROPS AND PRODUCTS
(2024)
Article
Agricultural Engineering
Marcos Vinicius Silva de Andrade, Simone Ribeiro Lucho, Renato Delmondez de Castro, Paulo Roberto Ribeiro
Summary: Stevia rebaudiana Bertoni, commonly known as stevia, is a widely cultivated shrub with highly sweetening metabolites. It has gained significant interest in the food and beverage industries due to its extremely sweet taste and various health benefits. Researchers are currently focused on studying biotechnological strategies to enhance the biosynthesis of stevia plant metabolites for improved cultivation and production.
INDUSTRIAL CROPS AND PRODUCTS
(2024)
Article
Agricultural Engineering
Eleonora Cintura, Paulina Faria, Luisa Molari, Luca Barbaresi, Dario D'Orazio, Lina Nunes
Summary: This study investigated the properties of Arundo donax as a building material and found that it has strong adaptability, high hygroscopicity, and unique sound absorption behavior. The mechanical tests also demonstrated the applicability of the Arundo donax-based composite. This opens up new possibilities for the further application of Arundo donax-based materials.
INDUSTRIAL CROPS AND PRODUCTS
(2024)
Article
Agricultural Engineering
Stiina Kotiranta, Juha-Matti Pihlava, Titta Kotilainen, Pauliina Palonen
Summary: The effect of light spectra, particularly far-red and short wavelength radiation, on the growth and secondary metabolites of cannabis is significant. Altering the R:FR ratio can increase the concentration and yield of secondary metabolites, while short wavelength radiation does not significantly affect plant morphology and secondary metabolite concentrations.
INDUSTRIAL CROPS AND PRODUCTS
(2024)
Article
Agricultural Engineering
Xuze Liu, Fangmin Liang, Jinyuan Cheng, Xuelian Zhou, Jia Wang, Guigan Fang, Kuizhong Shen, Xianzhi Meng, Chen Huang, Arthur J. Ragauskas
Summary: A novel Na2S-facilitated DES pretreatment method was proposed to fractionate moso bamboo, which achieved high lignin removal and preserved most cellulose and hemicellulose with excellent enzymatic digestibility.
INDUSTRIAL CROPS AND PRODUCTS
(2024)
Article
Agricultural Engineering
Zechun Ren, Xinyuan Zhou, Kejiao Ding, Tong Ji, Hao Sun, Xiang Chi, Yunzhao Wei, Min Xu
Summary: This study prepared a thermal/magnetic/light-triggered multi-stimuli responsive shape memory polymer composite with excellent properties by adding magnetic nanoparticles into a polyurethane composite. The composite showed high shape recovery rate, fast response behavior, and suitability for 3D printing. Controllable external stimuli allowed for dynamic, remote, and precise control of 4D transformations. This work is significant for the design of smart materials and devices.
INDUSTRIAL CROPS AND PRODUCTS
(2024)
Article
Agricultural Engineering
Yurany Camacho Ardila, Jaiver Efren Jaimes Figueroa, Maria Regina Wolf Maciel
Summary: The higher heating value (HHV) and elemental composition are important properties of biomass, and their prediction using proximate and ultimate analysis is accurate.
INDUSTRIAL CROPS AND PRODUCTS
(2024)
Article
Agricultural Engineering
Xiaohan Chen, Xianke Wang, Lili Shang, Xinxin Ma, Changhua Fang, Benhua Fei, Huanrong Liu, Shuqin Zhang
Summary: This study focuses on the comprehensive characterization of the three-dimensional (3D) structure of bamboo parenchyma and the evaluation of its tensile and compressive properties, as well as the analysis of its failure mechanisms. The study found that the measured tensile strength and elastic modulus of the parenchyma tissue are 48.81 MPa and 706.48 MPa, respectively. Compression failure modes in parenchyma tissue encompass compression bending and cell fracture, shear failure of the middle lamella, and detachment and debonding of the cell wall.
INDUSTRIAL CROPS AND PRODUCTS
(2024)
Article
Agricultural Engineering
Tianfeng Liu, Yuxin Liu, Yunlin Fu, Mengji Qiao, Penglian Wei, Zhigao Liu, Yingjian Li
Summary: This study explored the formation mechanism of agarwood induced by fungus and its potential application in high-quality and high-yielding agarwood production. The results showed that the transition layer played a critical role in the continuous accumulation of agarwood, and the parenchyma cells of the xylem rays and interxylary phloem were the main action site of fungus. The accumulation and distribution of chemical composition in different layers were also investigated. The study identified the drivers of specific compounds and provided a theoretical foundation for future agarwood production.
INDUSTRIAL CROPS AND PRODUCTS
(2024)