Journal
INDUSTRIAL CROPS AND PRODUCTS
Volume 41, Issue -, Pages 371-374Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.indcrop.2012.04.047
Keywords
Didehydrostemofoline; Extraction methods; Stemona collinsiae; Stemona; Stemonaceae
Categories
Funding
- Thailand Research Fund
- Mahidol University [PHD/0139/2550]
- Education Commission
- Mahidol University under the National Research Universities Initiative
Ask authors/readers for more resources
Stemona plants, which are popularly called Non Tai Yak in Thailand, have been traditionally used as a bio-pesticide. Stemona collinsiae is one of the most interesting species containing didehydrostemofoline alkaloid as a major component with promising insecticidal toxicity. The extraction methods yielding the highest content of didehydrostemofoline were investigated in S. collinsiae roots and the extracts were monitored with the validated-HPLC. Selection of a suitable extracting solvent was also taken into account. Using a single solvent, the yield of didehydrostemofoline was clearly increased with the increasing polarity (acetone < acetonitrile < ethanol < methanol). Methanol and 70% ethanol were the efficient solvents for extracting didehydrostemofoline from the roots with nearly the same yields of 0.393 +/- 0.007 and 0.388 +/- 0.001% w/w, respectively. Seventy percent ethanol, which is less hazardous than methanol, was recommended as the solvent for extracting S. collinsiae roots. Comparative analysis of didehydrostemofoline in the root extracts prepared by different extraction methods (sonication, reflux, Soxhlet, maceration, percolation) with 70% ethanol showed that reflux and sonication gave the highest content of didehydrostemofoline. However, reflux is simple, cheap, and more convenient for upscaled to industrial process. Therefore, it should be recommended for extraction of high quality extracts from S. collinsiae roots. This work will be useful as a guide for further preparation and development of S. collinsiae root extracts as natural pharmaceutical and/or insecticidal products. (C) 2012 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available