4.7 Article

The extent of genetic diversity among Vanilla species: Comparative results for RAPD and ISSR

Journal

INDUSTRIAL CROPS AND PRODUCTS
Volume 29, Issue 2-3, Pages 581-589

Publisher

ELSEVIER
DOI: 10.1016/j.indcrop.2008.11.006

Keywords

Vanilla; RAPD; ISSR; Polymorphic markers

Ask authors/readers for more resources

Vanilla is a large genus of about 110 species in the orchid family (Orchidaceae), including the species Vanilla planifolia from which commercial vanilla flavoring is derived. Since most species of vanilla are considered rare and endangered there is an urgent need to conserve them through genetic analysis and propagation/conservation studies on this crop. The present study investigated the genetic diversity among nine leafy- and leaf-less vanilla species employing 30 decamer RAPD primers and 10 ISSR primers. The species under study were diverse and displayed a range of variability (0-66% and 0-81% for RAPD and ISSR, respectively). A total of 154 RAPD polymorphic markers (83.24%, h = 0.378) and 93 ISSR polymorphic markers (86.11%, h = 0.363) were used to generate a genetic similarity matrix followed by them cluster analysis. Specific groupings were revealed by each cluster analysis with slight Variation between two different markers. Among the nine species studied, V planifolia, Vanilla aphylla and Vanilla tahitensis revealed very low level of variation within their collections, thus indicating a narrow genetic base. The large genetic distance of Vanilla andamanica from other species suggests its different origin. A close genetic affinity was observed between the pairs V. planifolia, V. tahitensis and Vanilla albida, V aphylla. These are the first comparative results for RAPD and ISSR reporting inter-relationship among nine cultivated, wild and hybrid Vanilla species. (C) 2008 Elsevier B.V. All rights reserved.

Authors

I am an author on this paper
Click your name to claim this paper and add it to your profile.

Reviews

Primary Rating

4.7
Not enough ratings

Secondary Ratings

Novelty
-
Significance
-
Scientific rigor
-
Rate this paper

Recommended

Review Biochemistry & Molecular Biology

A Review of Chemistry and Pharmacology of Piperidine Alkaloids of Pinus and Related Genera

Lav Singh, Atul K. Upadhyay, Pooja Dixit, Arpit Singh, Divyanshu Yadav, Apurv Chhavi, Suraj Konar, Ravi Prakash Srivastava, Shivaraman Pandey, Hari Prasad Devkota, Praveen C. Verma, Gauri Saxena

Summary: This study presents the clinical applications and different structural types of piperidine alkaloids in the family Pinaceae, with special emphasis on the importance of the piperidine nucleus. By studying these alkaloids, researchers can create drugs with the highest therapeutic efficacy.

CURRENT PHARMACEUTICAL BIOTECHNOLOGY (2022)

Article Plant Sciences

Genotype-dependent and temperature-induced modulation of secondary metabolites, antioxidative defense and gene expression profile in Solanum viarum Dunal

Preeti Patel, Archana Prasad, Dipali Srivastava, Abhishek Niranjan, Gauri Saxena, Satya Shila Singh, Pratibha Misra, Debasis Chakrabarty

Summary: The study demonstrated that the prickleless genotype of Solanum viarum exhibited higher accumulation of secondary metabolites and biomass under heat and cold stress, along with a stronger antioxidative mechanism compared to the prickled genotype.

ENVIRONMENTAL AND EXPERIMENTAL BOTANY (2022)

Article Plant Sciences

A tau class GST, OsGSTU5, interacts with VirE2 and modulates the Agrobacterium-mediated transformation in rice

Madhu Tiwari, Neelam Gautam, Yuvraj Indoliya, Maria Kidwai, Arun Kumar Mishra, Debasis Chakrabarty

Summary: This study identified OsGSTU5 as a cellular interactor of VirE2 in rice and established its important role in modulating Agrobacterium-mediated transformation efficiency in rice.

PLANT CELL REPORTS (2022)

Article Environmental Sciences

Nitric oxide-mediated alleviation of arsenic stress involving metalloid detoxification and physiological responses in rice (Oryza sativa L.)

Pradyumna Kumar Singh, Debasis Chakrabarty, Sanjay Dwivedi, Amit Kumar, Surendra Pratap Singh, Geetgovind Sinam, Abhishek Niranjan, Poonam C. Singh, Sandipan Chatterjee, Dipanjali Majumdar, Manish Tiwari, Rudra Deo Tripathi

Summary: Rice, as a staple crop, is at risk of arsenic contamination, which poses a serious health threat. This study explores the protective role of nitric oxide (NO) in rice plants under arsenic stress. Exogenous NO supplementation protects the plant by regulating physiological traits and metabolic responses, reducing arsenic toxicity.

ENVIRONMENTAL POLLUTION (2022)

Article Engineering, Environmental

A tau class glutathione-S-transferase (OsGSTU5) confers tolerance against arsenic toxicity in rice by accumulating more arsenic in root

Madhu Tiwari, Maria Kidwai, Prasanna Dutta, Shiv Narayan, Neelam Gautam, Khushboo Chawda, Pramod Arvind Shirke, Arun Kumar Mishra, Debasis Chakrabarty

Summary: Arsenic is a hazardous metalloid that hampers physiological activities in rice. In this study, the role of OsGSTU5 in arsenic tolerance was investigated. OsGSTU5 was found to enhance arsenic tolerance by regulating antioxidant activities and participating in arsenic chelation and transport processes.

JOURNAL OF HAZARDOUS MATERIALS (2022)

Review Plant Sciences

Agrobacterium-mediated gene transfer: recent advancements and layered immunity in plants

Madhu Tiwari, Arun Kumar Mishra, Debasis Chakrabarty

Summary: Plants respond to Agrobacterium infection by mounting a three-layered immune response, which determines their susceptibility or resistance to the pathogen. Understanding the molecular dialogues between plants and Agrobacterium may provide important insights for the treatment of crown gall disease.

PLANTA (2022)

Review Integrative & Complementary Medicine

Overview of COVID-19 Pandemic: Its Management and Prevention in Light of the Indian Traditional Medicine System

Lav Singh, Arpit Singh, Divyanshu Yadav, Apurv Chhavi, Ravi Prakash Srivastava, Shivaraman Pandey, Pooja Dixit, Praveen Chandra Verma, Gauri Saxena

Summary: India's healthcare system is under pressure with one doctor serving 1456 people, far from the World Health Organization's recommended ratio of one doctor per 1000 population. As a result, traditional remedies have become popular in India as a substitute for a proper medical system. With its rich diversity of medicinal herbs and drugs, India's 5000-year-old medication system has effectively managed health issues and is now playing a crucial role in combating COVID-19.

CURRENT TRADITIONAL MEDICINE (2023)

Article Biotechnology & Applied Microbiology

Biotic elicitor-induced changes in growth, antioxidative defense, and metabolites in an improved prickleless Solanum viarum

Archana Prasad, Preeti Patel, Abhishek Niranjan, Aradhana Mishra, Gauri Saxena, Satya Shila Singh, Debasis Chakrabarty

Summary: This study aimed to enhance the production of alkaloids and glycoalkaloids in Solanum viarum culture by adding microbial-based elicitors. The results showed that the treatment with 3% Trichoderma reesei and Bacillus tequilensis culture filtrate significantly increased biomass and yield. Quantitative real-time PCR expression analysis revealed that the abundance of gene transcripts involved in metabolite biosynthesis correlated with their accumulation in elicited plants.

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY (2022)

Article Biochemistry & Molecular Biology

Comparative transcriptional analysis of metabolic pathways and mechanisms regulating essential oil biosynthesis in four elite Cymbopogon spp.

Gurminder Kaur, Surjeet Kumar Arya, Babita Singh, Sanchita Singh, Sushmita, Gauri Saxena, Praveen C. Verma, Deepak Ganjewala

Summary: This study found marked differences in the gene expression patterns of four contrasting Cymbopogon species, which are related to terpenoid metabolism and can be used for classification and identification of Cymbopogon genotypes. The results have important implications for improving the essential oil yield and quality in Cymbopogon.

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2023)

Article Horticulture

Micropropagation of Hoya carnosa, H. kerrii, H. parasitica, and H. longifolia using tray-based floating and stationary hydroponic systems

Naushad Alam, Waseem Siddique, Manoj K. Mishra, Anukriti Pandey, Dhermendra K. Purshottam, Khuraijam Jibankumar Singh, Shri Krishna Tewari, Debasis Chakrabarty

Summary: This study aims to develop an affordable and easy-to-use hydroponic system for the conservation and mass production of four Hoya species. The optimal medium for root growth was determined to be 1/2 MS + IBA (2.5μM). Zinc sulfate (ZnSO4) at concentrations of 25, 50, 100, 150, and 200μM was found to improve root number. The findings of this study are important for the conservation and mass propagation of Hoya species.

SCIENTIA HORTICULTURAE (2023)

Review Pharmacology & Pharmacy

The industrially important genus Kaempferia: An ethnopharmacological review

Arpit Singh, Nitesh Singh, Sanchita Singh, Ravi Prakash Srivastava, Lav Singh, Praveen C. Verma, Hari P. Devkota, Laiq ur Rahman, Bikash Kumar Rajak, Amrita Singh, Gauri Saxena

Summary: Kaempferia, a widely distributed genus of the family Zingiberaceae, is highly valued for its ethnobotanical significance and has received increasing scholarly attention due to its numerous health advantages. This review compiles scientific information on the relevance, distribution, industrial applications, phytochemistry, ethnopharmacology, tissue culture, and conservation initiatives of Kaempferia, as well as the commercial realities and limitations of research. It also explores the future clinical potential of this genus. The knowledge from this study can contribute to the fields of phytopharmacology, molecular research, and industrial biology, and aid in the establishment of new conservation strategies.

FRONTIERS IN PHARMACOLOGY (2023)

Article Agronomy

Transcriptional Plasticity and Cell Wall Characterization in High-Methanol-Producing Transgenic Tobacco Plants

Sameer Dixit, Krishnappa Chandrashekar, Santosh Kumar Upadhyay, Praveen Chandra Verma

Summary: Volatile organic compounds from plant surfaces are a sustainable natural solution to combat biotic stresses. Leaf methanol, the second major volatile compound after isoprene, has been neglected for a long time but recent studies have shown its importance in development and stress responses.

AGRICULTURE-BASEL (2023)

Article Plant Sciences

DUF4057 containing express-protein negatively regulates the drought responses in rice

Poonam Tiwari, Puja Singh, Yuvraj Indoliya, Poonam C. Singh, Debasis Chakrabarty

Summary: This study conducted a functional characterization of a drought-responsive express-protein (OsEP) and found that it acts as a negative regulator in drought response in rice plants. Through regulating enzymatic activity, downregulation of the OsEP gene enables plants to adapt to drought conditions. Additionally, the study revealed the involvement of the GA-JA signaling pathway in the regulation of OsEP gene expression.

ENVIRONMENTAL AND EXPERIMENTAL BOTANY (2023)

Article Environmental Sciences

Phytoremediation potential of Solanum viarum Dunal and functional aspects of their capitate glandular trichomes in lead, cadmium, and zinc detoxification

Pragya Shukla, Maria Kidwai, Shiv Narayan, Pramod Arvind Shirke, Kapil Deo Pandey, Pratibha Misra, Debasis Chakrabarty

Summary: In the study, Solanum viarum Dunal was found to have the potential to accumulate hazardous heavy metals, including lead, cadmium, and zinc. The plant showed better germination percentage and accumulated high concentrations of these metals in its tissues under toxic conditions. It also exhibited positive modulation in physiological performances and antioxidant activities, suggesting enhanced tolerance and membrane stability. The study provides insights into the mechanisms that make S. viarum a potent accumulator and offers information for phytoremediation.

ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH (2023)

Article Biotechnology & Applied Microbiology

Interspecific and intraspecific analysis of Selinum spp. collected from Indian Himalayas using DNA barcoding

Ravi Prakash Srivastava, Gauri Saxena, Lav Singh, Arpit Singh, Praveen C. Verma, Gurminder Kaur

Summary: This study examines the universality of DNA barcoding using matK and rbcL markers in Selinum species. The success rates of PCR amplification and DNA sequencing suggest that these markers can be used for species identification. The phylogenetic analysis reveals that Selinum is closely related to Angelica species within the Apiaceae family.

JOURNAL OF GENETIC ENGINEERING AND BIOTECHNOLOGY (2022)

Article Agricultural Engineering

Structural changes of poplar lignin during the ternary deep eutectic solvent (DES) treatment and synergetic alkali-DES treatment

Jingmo Zhou, Shubin Wu, Ying Liu

Summary: Expenditure isolation of lignin and the understanding of its structural variations during different isolation processes are crucial for the high-value utilization of lignin. This study proposed a green deep eutectic solvent (DES) consisting of choline chloride, ethylene glycol, and 4-chlorobenzene sulfonic acid for rapid lignin separation from poplar. The structural variations of DES lignin and its relationship with alkali pre-extraction of hemicellulose were investigated. The results showed that alkali pretreatment enhanced the DES separation of lignin and promoted the cleavage of specific linkages, resulting in reduced molecular weight and thermal stability of isolated DES lignin. Moreover, DES lignin obtained from integrated utilization displayed higher purity and S/G ratio compared to lignin obtained from direct DES treatment.

INDUSTRIAL CROPS AND PRODUCTS (2024)

Article Agricultural Engineering

Integrated metabolomic and transcriptomic analysis provides insights into the flavonoid formation in different Glycyrrhiza species

Yuping Li, Ziyan Xie, Yun Huang, Jiangyi Zeng, Chao Yang, Ling Yuan, Ying Wang, Yongqing Li

Summary: In this study, the characteristic flavonoids (CFs) of three licorice species were identified through integrated metabolic and transcriptomic analysis. The key regulators of CF biosynthesis were also identified. These findings provide valuable information for understanding the biosynthesis of CFs and future breeding of Glycyrrhiza varieties.

INDUSTRIAL CROPS AND PRODUCTS (2024)

Article Agricultural Engineering

Construction of robust and fully bio-based epoxy vitrimers with facile malleability, self-healing ability, and recyclability from peach gum polysaccharide and itaconic acid

Xianjie Pan, Zhaoyi Luo, Fanqi Liu, Quanqian Yi, Yanning Zeng

Summary: This study presents a novel strategy for fabricating fully bio-based epoxy vitrimers using peach gum and itaconic acid, which exhibit superior mechanical properties and thermostability. The materials were characterized and the self-healing and recycling capabilities were evaluated. The results demonstrate the potential of using these materials for sustainable industrial applications.

INDUSTRIAL CROPS AND PRODUCTS (2024)

Article Agricultural Engineering

Colonization of Satureja hortensis L. (Summer savory) with Trichoderma harzianum alleviates salinity stress via improving physio-biochemical traits and biosynthesis of secondary metabolites

Mohsen Sabzi-Nojadeh, Mohammad Pouresmaeil, Mina Amani, Mehdi Younessi-Hamzekhanlu, Filippo Maggi

Summary: This study shows that inoculating Summer savory plants with endophytic fungus Trichoderma harzianum can improve plant growth and increase salt tolerance. The fungus enhances growth, restores chlorophyll and antioxidant levels, reduces harmful substances, and increases proline and soluble sugar levels, thereby increasing crop productivity in saline conditions.

INDUSTRIAL CROPS AND PRODUCTS (2024)

Article Agricultural Engineering

Sustainable fragrance release wax oil coating for wood substrate based on peppermint essential oil microcapsules

Yu Liu, Lei Li, Aichen Zhao, Xiuming Song, Lulu Wei, Mengdi Fang, Chuchu Zheng, Xiaodong Zhu

Summary: This study investigates the impact of peppermint essential oil microcapsules on the unpleasant odor emissions from wax oil coated wood products. The results show that microcapsules can improve the time and stability of odor release.

INDUSTRIAL CROPS AND PRODUCTS (2024)

Article Agricultural Engineering

Land suitability assessment for rapeseed potential cultivation in upper Tigris basin of Turkiye comparing fuzzy and boolean logic

Mesut Budak, Mirac Kilic, Hikmet Guenal, Ismail Velik, Mesut Sirri

Summary: This study assessed land suitability for rapeseed production using topography, climate, and soil data by analytical hierarchy process (AHP) and the Mamdani Fuzzy Inference System (MFIS). The study area covered land in the Diyarbakir province of southeastern Turkiye. The results indicated the proportions of different land suitability classes and showed the compatibility of AHP and MFIS methods in assessing suitability.

INDUSTRIAL CROPS AND PRODUCTS (2024)

Article Agricultural Engineering

Strong, UV-blocking, and hydrophobic PVA composite films containing tunable lignin nanoparticles

Rui Tian, Yue Liu, Chao Wang, Weikun Jiang, Srinivas Janaswamy, Guihua Yang, Xingxiang Ji, Gaojin Lyu

Summary: In this study, hollow and solid lignin nanoparticles were synthesized through self-assembly and their application in PVA nanocomposite films was investigated. The results showed that the presence of hollow lignin nanoparticles in the films resulted in a room-like network structure, while the solid lignin nanoparticles induced different structural changes. The inclusion of LNPs improved the mechanical properties of the films, and both types of LNPs exhibited good hydrophobicity.

INDUSTRIAL CROPS AND PRODUCTS (2024)

Article Agricultural Engineering

Alternative for natural sweeteners: Improving the use of stevia as a source of steviol glycosides

Marcos Vinicius Silva de Andrade, Simone Ribeiro Lucho, Renato Delmondez de Castro, Paulo Roberto Ribeiro

Summary: Stevia rebaudiana Bertoni, commonly known as stevia, is a widely cultivated shrub with highly sweetening metabolites. It has gained significant interest in the food and beverage industries due to its extremely sweet taste and various health benefits. Researchers are currently focused on studying biotechnological strategies to enhance the biosynthesis of stevia plant metabolites for improved cultivation and production.

INDUSTRIAL CROPS AND PRODUCTS (2024)

Article Agricultural Engineering

Characterization of an Arundo donax-based composite: A solution to improve indoor comfort

Eleonora Cintura, Paulina Faria, Luisa Molari, Luca Barbaresi, Dario D'Orazio, Lina Nunes

Summary: This study investigated the properties of Arundo donax as a building material and found that it has strong adaptability, high hygroscopicity, and unique sound absorption behavior. The mechanical tests also demonstrated the applicability of the Arundo donax-based composite. This opens up new possibilities for the further application of Arundo donax-based materials.

INDUSTRIAL CROPS AND PRODUCTS (2024)

Article Agricultural Engineering

The morphology, inflorescence yield, and secondary metabolite accumulation in hemp type Cannabis sativa can be influenced by the R:FR ratio or the amount of short wavelength radiation in a spectrum

Stiina Kotiranta, Juha-Matti Pihlava, Titta Kotilainen, Pauliina Palonen

Summary: The effect of light spectra, particularly far-red and short wavelength radiation, on the growth and secondary metabolites of cannabis is significant. Altering the R:FR ratio can increase the concentration and yield of secondary metabolites, while short wavelength radiation does not significantly affect plant morphology and secondary metabolite concentrations.

INDUSTRIAL CROPS AND PRODUCTS (2024)

Article Agricultural Engineering

Investigation of a Na2S-assisted deep eutectic solvent pretreatment for enhanced cellulose/xylan saccharification and high-quality lignin with abundant ether bond

Xuze Liu, Fangmin Liang, Jinyuan Cheng, Xuelian Zhou, Jia Wang, Guigan Fang, Kuizhong Shen, Xianzhi Meng, Chen Huang, Arthur J. Ragauskas

Summary: A novel Na2S-facilitated DES pretreatment method was proposed to fractionate moso bamboo, which achieved high lignin removal and preserved most cellulose and hemicellulose with excellent enzymatic digestibility.

INDUSTRIAL CROPS AND PRODUCTS (2024)

Article Agricultural Engineering

Advancing multi-stimuli 4D printing of polylactic acid with rapid shape memory and enhanced toughness using lignin-coated Fe3O4 nanoparticles

Zechun Ren, Xinyuan Zhou, Kejiao Ding, Tong Ji, Hao Sun, Xiang Chi, Yunzhao Wei, Min Xu

Summary: This study prepared a thermal/magnetic/light-triggered multi-stimuli responsive shape memory polymer composite with excellent properties by adding magnetic nanoparticles into a polyurethane composite. The composite showed high shape recovery rate, fast response behavior, and suitability for 3D printing. Controllable external stimuli allowed for dynamic, remote, and precise control of 4D transformations. This work is significant for the design of smart materials and devices.

INDUSTRIAL CROPS AND PRODUCTS (2024)

Article Agricultural Engineering

Mathematical models for predicting the higher heating value and ultimate analysis of biomass

Yurany Camacho Ardila, Jaiver Efren Jaimes Figueroa, Maria Regina Wolf Maciel

Summary: The higher heating value (HHV) and elemental composition are important properties of biomass, and their prediction using proximate and ultimate analysis is accurate.

INDUSTRIAL CROPS AND PRODUCTS (2024)

Article Agricultural Engineering

Three-dimensional structural characterization and mechanical properties of bamboo parenchyma tissue

Xiaohan Chen, Xianke Wang, Lili Shang, Xinxin Ma, Changhua Fang, Benhua Fei, Huanrong Liu, Shuqin Zhang

Summary: This study focuses on the comprehensive characterization of the three-dimensional (3D) structure of bamboo parenchyma and the evaluation of its tensile and compressive properties, as well as the analysis of its failure mechanisms. The study found that the measured tensile strength and elastic modulus of the parenchyma tissue are 48.81 MPa and 706.48 MPa, respectively. Compression failure modes in parenchyma tissue encompass compression bending and cell fracture, shear failure of the middle lamella, and detachment and debonding of the cell wall.

INDUSTRIAL CROPS AND PRODUCTS (2024)

Article Agricultural Engineering

Structural, defense enzyme activity and chemical composition changes in the xylem of Aquilaria sinensis during fungus induction

Tianfeng Liu, Yuxin Liu, Yunlin Fu, Mengji Qiao, Penglian Wei, Zhigao Liu, Yingjian Li

Summary: This study explored the formation mechanism of agarwood induced by fungus and its potential application in high-quality and high-yielding agarwood production. The results showed that the transition layer played a critical role in the continuous accumulation of agarwood, and the parenchyma cells of the xylem rays and interxylary phloem were the main action site of fungus. The accumulation and distribution of chemical composition in different layers were also investigated. The study identified the drivers of specific compounds and provided a theoretical foundation for future agarwood production.

INDUSTRIAL CROPS AND PRODUCTS (2024)