Journal
INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
Volume 53, Issue 42, Pages 16176-16182Publisher
AMER CHEMICAL SOC
DOI: 10.1021/ie5021206
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Funding
- National Natural Science Foundation of China (NSFC) [21072041]
- Open Funding Project of the National Key Laboratory of Biochemical Engineering [KF2010-12]
- Foundation of the Key Laboratory of Industrial Fermentation Microbiology of the Ministry of Education [2012IM004]
- Tianjin Key Lab of Industrial Microbiology (Tianjin University of Science Technology)
- Natural Science Foundation of Hebei Province [B2014208054]
- Foundation of Hebei University of Science and Technology [SW10]
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With the aim of providing a highly stable and easy recyclable biocatalyst, we prepared a new biocatalyst formulation based on cross-linked enzyme aggregate (CLEA) technology by the physical adsorption of cross-linked phenylalanine ammonia lyase on a highly porous microspherical silica gel solid support (S-CLEAs). The main factors in the preparation of S-CLEAs were optimized by response surface methodology (RSM). Analysis by scanning electron microscopy (SEM), FT-IR spectroscopy, and confocal laser scanning microscopy (CLSM) indicated that the CLEAs were successfully immobilized on the crude-pored microspherical silica gel. Compared to standard CLEAs, the S-CLEAs can be easily separated from the reaction mixture by natural precipitation without centrifugation and filtration treatments. Although the S-CLEAs revealed an excellent stability of the enzyme against high temperature and denaturants, the volumetric activity of the S-CLEAs decreased because of the presence of silica gel. In a word, the S-CLEA technology in this study might open an attractive route toward effectively improving the catalytic properties of traditional CLEAs.
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