Journal
INDIAN JOURNAL OF MICROBIOLOGY
Volume 54, Issue 2, Pages 239-241Publisher
SPRINGER
DOI: 10.1007/s12088-013-0425-9
Keywords
Medium optimization; Plant growth-promoting bacteria; Pseudomonas trivialis
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Funding
- CSIR, Govt. of India under the CSIR Network Project Exploitation of India's Rich Microbial Diversity
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Optimizing nutritional requirements for mass production of microbial inoculants in shortened time has relevance for their economical field application. Therefore, the present study aimed at selecting suitable growth medium, optimizing its components, and up-scaling inoculum production for plant growth-promoting Pseudomonas trivialis BIHB 745. Of the different media tested, the culture exhibited maximal viable colony count in trypticase soya broth with 17.6 % increased biomass on optimizing levels of carbon source, nitrogen source, and NaCl using response surface methodology. A twofold higher biomass with 9 h shorter incubation period was obtained in optimized medium in a bioreactor in comparison to shake flasks.
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