Journal
INDIAN JOURNAL OF MEDICAL MICROBIOLOGY
Volume 28, Issue 1, Pages 57-59Publisher
ELSEVIER
DOI: 10.4103/0255-0857.58732
Keywords
DNA; slit skin smear; polymerase chain reaction; leprosy
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Funding
- The Leprosy Mission, India
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Identification of Mycobacterium leprae, which causes leprosy, is done by Ziehl Neelsen Carbol Fuchsin (ZNCF) stained slit skin smear microscopy that aids in the diagnosis and quantification of approximate bacterial load carried by the patient. We attempted M. leprae DNA extraction from 46 stained slit skin smear negative slides, using Proteinase K and SDS lysis, followed by ethanol precipitation. M. leprae specific primers (16SrRNA) were used for PCR-based amplification of DNA. We could detect M. leprae DNA in 15 (32.6) samples. The method can be useful in the diagnosis of apparently slit skin smear negative leprosy cases.
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