Article
Plant Sciences
Alejandra Rojas-Vargas, Ander Castander-Olarieta, Antonia Maiara Marques do Nascimento, Maria Laura Velez, Catia Pereira, Joao Martins, Monica Zuzarte, Jorge Canhoto, Itziar A. A. Montalban, Paloma Moncalean
Summary: This study aimed to improve the protocols for P. ponderosa regeneration through in vitro organogenesis by analyzing the influence of different explants, culture media, cytokinins, auxins, and light treatments on shoot multiplication and rooting phases. The results showed that whole zygotic embryos and 44 mu M 6-benzyladenine had the best survival rate for explants. Blue LED light treatment promoted shoot elongation, while fluorescent light treatment improved acclimatization percentage.
Article
Biotechnology & Applied Microbiology
Sandopu Sravan Kumar, Parvatam Giridhar
Summary: A novel and efficient in vitro micropropagation protocol was established for B. rubra shoot cultures without the use of plant growth regulators, achieving 100% elongation and rooting of microshoots. Seed germination was improved to 70% with urea treatment, and multiple shoot proliferation was initiated using nodal shoot segments from mature plants in pots. Multiple shoots were obtained with specific growth regulator combinations, and successful rooting was achieved in both MS medium and MS medium supplemented with activated charcoal.
PLANT CELL TISSUE AND ORGAN CULTURE
(2021)
Article
Horticulture
Alessandra Sgueglia, Andrea Frattarelli, Adele Gentile, Gaia Urbinati, Simona Lucioli, Maria Antonietta Germana, Emilia Caboni
Summary: Cryopreservation by droplet vitrification was applied to hazelnut axillary buds, with the highest regrowth percentage obtained after applying PVS3 for 60 minutes. Cold pre-treatment on the mother plant did not significantly improve overall regrowth, and the cryopreservation process did not decline the rooting ability of the recovered shoots.
Article
Biochemistry & Molecular Biology
Yuan-Yuan Li, Zhi-Gang Hao, Shuo Miao, Xiong Zhang, Jian-Qiang Li, Shun-Xing Guo, Yung- Lee
Summary: This study identified key CKs metabolic genes from the Phalaenopsis genome and investigated their expression profiles and endogenous CKs dynamics under thidiazuron (TDZ) treatments. The results showed that TDZ treatment promoted shoot proliferation in Phalaenopsis and significantly increased the levels of CK-ribosides. Overexpression of certain CKs metabolic genes resulted in increased or decreased levels of CK-ribosides, suggesting their regulatory roles in CKs metabolism. These findings provide a better understanding of CKs metabolism in Phalaenopsis micropropagation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Plant Sciences
Sumira Tyub, Shabeer Ahmad Dar, Ishfaq Maqbool Lone, Aabid Hussain Mir, Azra N. Kamili
Summary: The study successfully developed a rapid in vitro propagation protocol for Echinacea angustifolia, regenerating complete plantlets from various explants. The highest shoot induction rate was observed in shoot tip explants, with other explants also showing successful shoot regeneration. This optimized protocol has the potential for mass production of this medicinal plant.
CURRENT PLANT BIOLOGY
(2021)
Article
Horticulture
Nevena Banjac, Dijana Krstic-Milosevic, Tatjana Mijalkovic, Mirjana Petrovic, Tatjana Cosic, Mariana Stanisic, Branka Vinterhalter
Summary: In this study, an efficient tissue culture system for improving the rocket cultivar Domaca rukola was established. Optimal shoot multiplication and organogenesis protocols were determined through experiments with different hormone concentrations and combinations. In addition, in vitro-produced rocket shoots were found to be a potential source of the antioxidant flavonoid kaempferol.
Article
Biotechnology & Applied Microbiology
Vanessa Alves, Rui Pinto, Clayton Debiasi, Maria Conceicao Santos, Jose Carlos Goncalves, Joana Domingues
Summary: Corema album, a shrub with edible, ornamental and medicinal value, has a limited distribution and slow regeneration rate in its natural habitat. A novel in vitro regeneration protocol has been developed for the first time from adult plants in the field, showing promise for future breeding and valorization efforts.
PLANT CELL TISSUE AND ORGAN CULTURE
(2021)
Article
Horticulture
Huda E. Mahood, Majeed Kadhem Abbas, Nisar Ahmad Zahid
Summary: This study established a micropropagation protocol for feverfew and quantified the parthenolide content in micropropagated and conventionally grown plants. The results showed that micropropagated plants had significantly higher parthenolide levels compared to conventionally grown plants, indicating that micropropagation can be used to produce disease-free planting materials and improve the parthenolide content of feverfew.
Article
Plant Sciences
Klaus-Peter Goetz, Frank-M Chmielewski, Danuse Tarkowska, Ales Pencik, Ondrej Novak
Summary: This study was conducted to assess the involvement of gibberellins (GAs), cytokinins (CKs), and auxins (AX) in the dormancy of sweet cherry buds. The results showed that these hormone groups did not exhibit significant changes during the transition between different dormancy phases. However, there were specific changes in the content of bioactive molecules during ontogenetic development.
JOURNAL OF PLANT GROWTH REGULATION
(2023)
Article
Forestry
Maurizio Capuana, Werther Guidi Nissim, Joshua D. Klein
Summary: We describe a micropropagation protocol for three ecotypes of S. acmophylla, which showed that the best results for shoot proliferation were obtained by culturing the shoot explants on Woody Plant Medium containing benzyladenine. Separating the shoot tip from the underlying internodes further enhanced proliferation.
Article
Forestry
Alejandra Rojas-Vargas, Ander Castander-Olarieta, Itziar A. Montalban, Paloma Moncalean
Summary: Genetic improvement programs for conifer forest species face challenges in propagating elite individuals and addressing the effects of climate change. This study analyzes the influence of collection dates, 6-benzyladenine concentrations, and light types on shoot and root induction in Pinus radiata. The study also examines the impact of these factors on amino acid and carbohydrate content in vitro. The results highlight the importance of genotype and environmental factors in successful regeneration of conifer species.
Article
Biotechnology & Applied Microbiology
Alejandra Hernandez-Garcia, Enrique Ambriz-Parra, Pablo Lopez-Albarran, Jose Cruz-de Leon, Rafael Salgado-Garciglia
Summary: The endangered species Dalbergia congestiflora was successfully propagated using an in-vitro micropropagation technique, with optimized growth conditions under different hormonal treatments. The study found that a higher shoot number was obtained on MS supplemented with 1 mgl(-1) BA and 0.1 mgl(-1) NAA, and roots could be developed without the addition of IBA.
PLANT BIOTECHNOLOGY
(2021)
Article
Forestry
Catia Pereira, Itziar A. Montalban, Ana Pedrosa, Jessica Tavares, Alexey Pestryakov, Nina Bogdanchikova, Jorge Canhoto, Paloma Moncalean
Summary: Organogenesis and somatic embryogenesis are two main regeneration pathways in plant tissue cultures, but the difficulty in obtaining somatic embryos remains a challenge for cloning mature trees. This study attempted to induce organogenesis and somatic embryogenesis in mature Aleppo pine trees through the culture of apical shoot buds, resulting in the production of embryogenic-like tissue and non-embryogenic calli. While somatic embryos could not be obtained, phenotypically normal plants were successfully regenerated through organogenesis.
Article
Plant Sciences
Jana Sediva, Jiri Velebil, Daniel Zahradnik
Summary: The aim of this study was to develop a successful micropropagation protocol for selected Sorbus species, evaluating shoot induction and rooting ability. Significant differences were found between the species, with N-6-benzyladenine showing a greater effect on shoot regeneration. Root induction was key to successful micropropagation, with auxin-untreated microcuttings of three species rooting better than those treated with indole-3-butyric acid. This is the first published micropropagation protocol for S. omissa, S. x abscondita and S. x kitaibeliana.
Article
Biotechnology & Applied Microbiology
Jie Zhou, Fengling Guo, Chuangdong Qi, Jiaping Fu, Ying Xiao, Jinping Wu
Summary: This study aimed to optimize the protocol for acclimatization of tissue culture ginger plantlets. By adjusting the conditions of light, temperature, humidity, and substrate, successful survival and rooting of tissue culture ginger plantlets were achieved. This has important implications for commercial production of ginger.
PLANT CELL TISSUE AND ORGAN CULTURE
(2022)
Article
Plant Sciences
L. A. Elkonin, J. V. Italianskaya, I. V. Domanina, N. Yu. Selivanov, A. L. Rakitin, N. V. Ravin
RUSSIAN JOURNAL OF PLANT PHYSIOLOGY
(2016)
Article
Agronomy
L. A. Elkonin, J. V. Italianskaya, I. Yu. Fadeeva, V. V. Bychkova, V. V. Kozhemyakin
Article
Agronomy
J. V. Italianskaya, L. A. Elkonin, V. V. Kozhemyakin
Article
Genetics & Heredity
L. A. Elkonin, M. I. Tsvetova
RUSSIAN JOURNAL OF GENETICS
(2008)
Article
Genetics & Heredity
L. A. Elkonin, G. A. Gerashchenkov, M. I. Tsvetova, N. A. Rozhnova
RUSSIAN JOURNAL OF GENETICS
(2010)
Article
Genetics & Heredity
L. A. Elkonin, E. V. Belyaeva, I. Yu Fadeeva
RUSSIAN JOURNAL OF GENETICS
(2012)
Article
Plant Sciences
L. A. Elkonin, M. I. Tsvetova
FRONTIERS IN PLANT SCIENCE
(2012)
Article
Plant Sciences
Elena V. Belyaeva, Lev A. Elkonin, Anastasia A. Vladimirova, Valery M. Panin
Summary: The AS-3 line of Sorghum bicolor exhibits functional components of apomixis such as apospory, parthenogenesis, and autonomous endospermogenesis. Genetic selection for these apomixis components in this diploid species of cultivated crops has proven to be efficient.
Article
Agriculture, Multidisciplinary
O. P. Kibalnik, L. A. Elkonin
VAVILOVSKII ZHURNAL GENETIKI I SELEKTSII
(2020)
Article
Plant Sciences
Marina Tsvetova, Lev Elkonin, Yulia Italianskaya
PHYTON-INTERNATIONAL JOURNAL OF EXPERIMENTAL BOTANY
(2019)
Article
Agriculture, Multidisciplinary
L. A. Elkonin, V. V. Kozhemyakin, M. I. Tsvetova
VAVILOVSKII ZHURNAL GENETIKI I SELEKTSII
(2019)
Meeting Abstract
Plant Sciences
Lev Elkonin, Yulia Italyanskaya, Valery Panin, Nikolay Selivanov, Andrey Rakitin
IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT
(2018)
Article
Agronomy
Valentin V. Kozhemyakin, Lei A. Elkoni, Jeffery A. Dahlberg
Article
Agronomy
LA Elkonin, VV Kozhemyakin, AG Ishin