Article
Multidisciplinary Sciences
Fang Gao, Xi Cao, Caiyun Qin, Shigang Chen, Jufeng Cai, Changbin Sun, Lisheng Kong, Jing Tao
Summary: Embryogenic tissue (ET) is crucial for genetic modification and plant re-generation. This study induced ET from mature zygotic embryos in blue spruce and investigated the effects of different factors on ET proliferation and somatic embryo (SE) maturation. The specific concentrations of 2,4-D and BA during the ET proliferation stage significantly affected both ET growth and the quality of ET for SE maturation. Developing genotype-specific protocols is necessary for efficient production of mature SEs or somatic plants in blue spruce.
SCIENTIFIC REPORTS
(2023)
Article
Plant Sciences
Aigul Amirova, Symbat Dossymbetova, Yeldana Rysbayeva, Bakdaulet Usenbekov, Arman Tolegen, Alibek Ydyrys
Summary: The aim of this study was to investigate the effect of plant growth regulators on callus induction and in vitro morphogenesis of Paulownia tomentosa and develop an efficient plant regeneration protocol. It was found that the addition of TDZ and NAA stimulated somatic embryogenesis, while the combination of BAP and NAA was optimal for callus induction and multiple plant regeneration. The regenerative capacity of P. tomentosa varied depending on the type of explant, occurring through both callus formation and direct regeneration. Overall, this study successfully developed a reproducible protocol for embryogenic callus formation and multiple shoot induction in vitro, leading to an increased frequency of plant regeneration.
Article
Multidisciplinary Sciences
Hyun-A Woo, Seong Sub Ku, Eun Yee Jie, HyeRan Kim, Hyun-Soon Kim, Hye Sun Cho, Won-Joong Jeong, Sang Un Park, Sung Ran Min, Suk Weon Kim
Summary: This study revealed that the highest frequency of embryogenic callus formation from immature zygotic embryos of Euonymus alatus was achieved with 1 mg/L 2,4-D on MS medium. Moreover, 3% (w/v) sucrose treatment was found to be optimal for somatic embryo formation.
SCIENTIFIC REPORTS
(2021)
Article
Horticulture
Yu Liu, Ying Zhan, Qiang Fu, Songlin Li, Xinyu Sun, Yaru Wang, Min Yu, Dong Qin, Junwei Huo, Chenqiao Zhu
Summary: This study established an in vitro regeneration system for blue honeysuckle through somatic embryogenesis for the first time, and improved the previously established indirect organogenesis-based regeneration system. By optimizing different culture media, efficient induction of embryogenesis and organogenesis in blue honeysuckle can be achieved. This study provides a powerful tool for rapid propagation, transformation, and genetic improvement of blue honeysuckle.
Article
Plant Sciences
Fatiha Bradai, Carolina Sanchez-Romero
Summary: The cryopreservation of olive somatic embryos using droplet-vitrification method affected the somatic embryogenesis process and its outcomes varied depending on the genotype. Specifically, the Line T1 showed higher fresh weight increases and the Line P5 exhibited improved maturation pattern and germination results after cryopreservation. Genotype plays a key role in determining the impact of cryopreservation on olive somatic embryogenesis.
Review
Plant Sciences
Guifang Zhang, Wu Liu, Zewei Gu, Shasha Wu, E. Yilan, Wenkun Zhou, Jinxing Lin, Lin Xu
Summary: This review summarizes the important role of jasmonate in plant regeneration. Jasmonate acts as a hormone linking wounding to different types of regeneration by regulating stem cell activities, cell proliferation, cell fate transition, and auxin production.
JOURNAL OF EXPERIMENTAL BOTANY
(2023)
Article
Plant Sciences
Andrei A. Kudriashov, Natalia S. Zlydneva, Elena P. Efremova, Varvara E. Tvorogova, Ludmila A. Lutova
Summary: CLE peptides play a role in plant development and can be regulated by WOX transcription factors. This study identified three CLE peptides that could potentially regulate the MtWOX9-1 gene and stimulate somatic embryogenesis in Medicago truncatula. However, none of these peptides affected the expression of MtWOX9-1 in embryogenic calli. One CLE gene, MtCLE16, was found to decrease somatic embryogenesis intensity when overexpressed, while overexpression of MtCLE08 suppressed the expression of MtWOX13a, a supposed antagonist of somatic embryo development. These findings contribute to the search for new regeneration regulators for plant transformation.
Article
Plant Sciences
Lucas R. Xavier, Felipe A. Almeida, Vitor B. Pinto, Lucas Z. Passamani, Claudete Santa-Catarina, Goncalo A. de Souza Filho, Brian P. Mooney, Jay J. Thelen, Vanildo Silveira
Summary: This study used proteomics and phosphoproteomics to analyze signaling events and protein regulation during sugarcane somatic embryo differentiation. Differential metabolic pathways and phosphorylation events were identified between multiplication and differentiation stages. Predicted protein interactions and phosphorylation motifs revealed new regulatory mechanisms in somatic embryogenesis.
JOURNAL OF PLANT PHYSIOLOGY
(2022)
Article
Forestry
Momi Tsuruta, Tsuyoshi E. Maruyama, Saneyoshi Ueno, Takumi Kaneeda, Yoshinari Moriguchi
Summary: This study provides basic information on somatic embryo maturation, germination, and growth performance of pollen-free sugi somatic plantlets derived from different embryogenic cell lines (ECLs). Significant differences in growth rate among ECLs were observed, indicating varying abilities in somatic seedling development.
Article
Forestry
Jingli Yang, Da Yang, Wanqiu Lu, Xin Zhang, Miaomiao Ma, Guifeng Liu, Jing Jiang, Chenghao Li
Summary: An efficient protocol for somatic embryogenesis in Betula platyphylla was developed, resulting in successful regeneration of plantlets. The use of appropriate plant growth regulators was found to increase the frequency of embryogenic callus induction. This somatic embryogenesis system can regenerate plantlets from immature zygotic embryos in a relatively short period of time.
JOURNAL OF FORESTRY RESEARCH
(2021)
Article
Forestry
Miaomiao Ma, Xuhui Wang, Chunyan Zhang, Solme Pak, Hongran Wu, Jingli Yang, Chenghao Li
Summary: This study aimed to cryopreserve embryogenic tissues of hybrid larch to overcome the challenges posed by rapid growth rates of conifer embryogenic cultures. By systematically evaluating several parameters, an optimized protocol for cryopreservation was developed, resulting in high survival rates of embryogenic tissues. The results provide a comprehensive set of steps for long-term conservation of embryogenic tissues in hybrid larch and have broader implications for cryopreservation of other plant species.
Article
Biotechnology & Applied Microbiology
Chunxue Peng, Fang Gao, Hao Wang, Hailong Shen, Ling Yang
Summary: This study presents an improved method for generating Korean pine somatic embryos and establishing a cryopreservation protocol for embryogenic tissue. The enhanced procedure led to a higher yield of cotyledonary somatic embryos, while also preserving rare germplasm resources. Somatic embryogenesis technology shows potential for accelerating breeding and meeting future wood harvesting needs.
PLANT CELL TISSUE AND ORGAN CULTURE
(2021)
Article
Plant Sciences
Irina Mitrofanova, Natalia Ivanova, Tatyana Kuzmina, Olga Mitrofanova, Natalya Zubkova
Summary: The study examined the effects of growth regulators on the morphogenic capacity of 13 cultivars of clematis plants, demonstrating that optimal concentrations of BAP and TDZ can promote shoot regeneration and somatic embryo development. Histological analysis revealed active formation of adventitious buds and somatic embryos, with specific concentrations of BAP and TDZ showing the best results for micropropagation. Additionally, light intensity and temperature were found to influence somatic embryo formation and development.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Biotechnology & Applied Microbiology
Evelyn Lopes Dos Santos, Joao Carlos Bespalhok Filho, Fabricio Augusto Hansel, Joao Arthur Tikler Sousa, Celso Garcia Auer, Neusa Steiner, Juliana Degenhardt
Summary: This study evaluated the impact of genotypes on somatic embryogenesis and the potential role of 5-azacytidine (5-azaC). The findings showed that genotypes play a role in somatic embryogenesis, and 5-azaC can affect the proliferation and morphology of aged embryogenic masses.
PLANT CELL TISSUE AND ORGAN CULTURE
(2023)
Article
Biotechnology & Applied Microbiology
Hongcheng Fang, Yuhui Dong, Rui Zhou, Qingpeng Wang, Qicheng Duan, Changxi Wang, Yan Bao, Shengyi Xu, Xinya Lang, Shasha Gai, Rong Chen, Ke Qiang Yang
Summary: An excellent tissue culture system for walnut was developed, which can accelerate the genetic improvement of walnut by ensuring the efficient induction, proliferation, germination, and conversion of somatic embryos.
PLANT CELL TISSUE AND ORGAN CULTURE
(2022)
Article
Biotechnology & Applied Microbiology
Hyung-Woo Jeon, Jin-Seong Cho, Eung-Jun Park, Kyung-Hwan Han, Young-Im Choi, Jae-Heung Ko
PLANT BIOTECHNOLOGY JOURNAL
(2016)
Article
Plant Sciences
Van Phap Nguyen, Jin-Seong Cho, Young-Im Choi, Sang-Won Lee, Kyung-Hwan Han, Jae-Heung Ko
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2016)
Article
Plant Sciences
Van Phap Nguyen, Jin-Seong Cho, Ji-Hoon Lee, Min-Ha Kim, Young-Im Choi, Eung-Jun Park, Won-Chan Kim, Seongbin Hwang, Kyung-Hwan Han, Jae-Heung Ko
JOURNAL OF PLANT BIOLOGY
(2017)
Article
Multidisciplinary Sciences
Bosung Choi, Ritesh Ghosh, Mayank Anand Gururani, Gnanendra Shanmugam, Junhyun Jeon, Jonggeun Kim, Soo-Chul Park, Mi-Jeong Jeong, Kyung-Hwan Han, Dong-Won Bae, Hanhong Bae
SCIENTIFIC REPORTS
(2017)
Article
Plant Sciences
Phi-Yen Le, Hyung-Woo Jeon, Min-Ha Kim, Eung-Jun Park, Hyoshin Lee, Indeok Hwang, Kyung-Hwan Han, Jae-Heung Ko
Article
Agricultural Engineering
Jae-Heung Ko, Won-Chan Kim, Jong Hee Im, Joo-Yeol Kim, Sara Patterson, Kyung-Hwan Han
BIOMASS & BIOENERGY
(2018)
Article
Plant Sciences
Won-Chan Kim, Joo-Yeol Kim, Jae-Heung Ko, Hunseung Kang, Jungmook Kim, Kyung-Hwan Han
Article
Biochemistry & Molecular Biology
Won-Chan Kim, Ida-Barbara Reca, YongSig Kim, Sunchung Park, Michael F. Thomashow, Kenneth Keegstra, Kyung-Hwan Han
PLANT MOLECULAR BIOLOGY
(2014)
Article
Biotechnology & Applied Microbiology
Jin-Seong Cho, Hyung-Woo Jeon, Min-Ha Kim, The K. Vo, Jinsoo Kim, Eung-Jun Park, Young-Im Choi, Hyoshin Lee, Kyung-Hwan Han, Jae-Heung Ko
PLANT BIOTECHNOLOGY JOURNAL
(2019)
Article
Biochemistry & Molecular Biology
Min-Ha Kim, Jin-Seong Cho, Eung-Jun Park, Hyoshin Lee, Young-Im Choi, Eun-Kyung Bae, Kyung-Hwan Han, Jae-Heung Ko
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Article
Genetics & Heredity
Jong Hee Im, Jae-Heung Ko, Won-Chan Kim, Brent Crain, Daniel Keathley, Kyung-Hwan Han
Summary: MYB46 acts as a master switch for secondary cell wall biosynthesis, ensuring precise gene expression in critical tissues but being suppressed where secondary wall formation is not desired. The negative regulation of MYB46 by MPK6 during salt stress reveals a novel mechanism for plants to incorporate environmental signals into differential regulation of secondary wall biosynthesis.
Article
Biotechnology & Applied Microbiology
Jin-Seong Cho, Min-Ha Kim, Eun-Kyung Bae, Young-Im Choi, Hyung-Woo Jeon, Kyung-Hwan Han, Jae-Heung Ko
Summary: By expressing PtrMYB3 and PdGA20ox1 genes in transgenic plants, significant improvements in stem fresh weight, secondary wall thickening, and cellulose content were achieved, leading to enhanced woody biomass production and improved biofuel production efficiency.
BIOTECHNOLOGY FOR BIOFUELS
(2021)
Article
Plant Sciences
Jong Hee Im, Seungmin Son, Jae-Heung Ko, Kyung-Hwan Kim, Chung Sun An, Kyung-Hwan Han
Summary: The plant MPK cascade plays a crucial role in the plant's response to environmental stimuli and phytohormones, with H2O2 being identified as the signaling molecule for triggering the nuclear translocation of GmMPK6 during salt stress.
Article
Plant Sciences
Seungmin Son, Jong Hee Im, Jae-Heung Ko, Kyung-Hwan Han
Summary: Cellular sugar starvation and energy deprivation are important signals for live cells to trigger stress adaptation response. When exposed to cellular energy stress (ES) conditions, plants reconfigure metabolic pathways and restrict vegetative organ growth. The regulatory mechanism underlying this growth restriction response in plants is largely unknown.
Review
Biotechnology & Applied Microbiology
Jae-Heung Ko, Won-Chan Kim, Jin-Seong Cho, Young-Im Choi, Eung-Jun Park, Jong Hee Im, Okran Han, Daniel Keathley, Kyung-Hwan Han
BIOFUELS BIOPRODUCTS & BIOREFINING-BIOFPR
(2017)