Article
Cell Biology
Roni Sarel-Gallily, Nissim Benvenisty
Summary: This study investigates the changes in the status of the X chromosome between primed and naive human embryonic stem cells (hESCs). The findings suggest that naive hESCs reactivate XIST gene and show increased gene expression levels on the X chromosome. However, the reactivation of the X chromosome in naive hESCs is not uniform and presents a distinct eroded pattern.
Article
Cell & Tissue Engineering
Seong-Min Kim, Eun-Ji Kwon, Yun-Jeong Kim, Young-Hyun Go, Ji-Young Oh, Seokwoo Park, Jeong Tae Do, Keun-Tae Kim, Hyuk-Jin Cha
Summary: This study discovered the differential roles of Shp2 in naive and primed pluripotency and proposed the usage of iShp2 instead of iMek1 for the efficient maintenance and establishment of naive pluripotency.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Multidisciplinary Sciences
Yan Bi, Zhifen Tu, Jianfeng Zhou, Xuehao Zhu, Hong Wang, Shaorong Gao, Yixuan Wang
Summary: This study investigates the cell fate dynamics during the transition from primed state to naive pluripotency using a dual fluorescent reporter system. The integration of transcription profiles and chromatin accessibility landscape reveals the appearance of primitive endoderm and trophectoderm signatures in the transitioning subpopulations. The study provides insights into early embryogenesis and offers hints for embryo modeling-related studies.
NATURE COMMUNICATIONS
(2022)
Article
Materials Science, Multidisciplinary
Xin Zhao, Qiuyan Ruan, Yun Feng, Ziyun Ruan, Zhulian Wu, Deshun Shi, Fenghua Lu
Summary: This study investigated the effect of blastocyst stage on the isolation of buffalo embryonic stem cells (bESCs). The results showed that expanded blastocyst and hatched blastocyst were more suitable for bESCs isolation and could maintain pluripotency.
MICROSCOPY AND MICROANALYSIS
(2022)
Article
Multidisciplinary Sciences
Shengyong Yu, Chunhua Zhou, Jiangping He, Zhaokai Yao, Xingnan Huang, Bowen Rong, Hong Zhu, Shijie Wang, Shuyan Chen, Xialian Wang, Baomei Cai, Guoqing Zhao, Yuhan Chen, Lizhan Xiao, He Liu, Yue Qin, Jing Guo, Haokaifeng Wu, Zhen Zhang, Man Zhang, Xiaoyang Zhao, Fei Lan, Yixuan Wang, Jiekai Chen, Shangtao Cao, Duanqing Pei, Jing Liu
Summary: This study utilizes single-cell RNA sequencing to examine the transition process of mouse stem cells and uncovers an alternative route from primed to naive pluripotency. The researchers discover that the transition goes through a primordial germ cell-like intermediate state. Furthermore, they elucidate the molecular mechanisms involved in this process, including the role of DOT1L, Gata2, and Prdm1/Blimp1.
NATURE COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Christina McKee, Christina Brown, Shreeya Bakshi, Keegan Walker, Chhabi K. Govind, G. Rasul Chaudhry
Summary: The use of 3-D self-assembling scaffolds supports the maintenance of naive state characteristics of ESCs and long-term growth. Gene expression profiles and biological processes in 3-D grown cells show significant changes, with upregulation of genes associated with transcription and translation regulation, extracellular matrix organization, and chromatin remodeling. Additionally, upregulation of genes linked to Wnt and focal adhesion signaling, and downregulation of genes related to the p53 signaling pathway, provide insight into the possible mechanisms of self-renewal of naive ESCs stimulated by mechanical signals in the 3-D microenvironment.
Article
Cell Biology
Mingwen Wang, Jinfeng Xue, Chanyi Li, Lingbin Qi, Lan Nie, Zhigang Xue
Summary: The optimization of culture conditions is crucial for improving embryo development competence in IVF. Glucose, an important carbon source, has unclear effects on the embryo development microenvironment. LC-MS analysis revealed defined metabolites at different cleavage stages, with glycometabolism playing a critical role. Adding 3 mmol/L glucose significantly improved the development competence and upregulated lipid biosynthesis gene expression at the 2-Cell stage.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Shadi Tarazi, Alejandro Aguilera-Castrejon, Carine Joubran, Nadir Ghanem, Shand Ashouokhi, Francesco Roncato, Emilie Wildschutz, Montaser Haddad, Bernardo Oldak, Elidet Gomez-Cesar, Nir Livnat, Sergey Viukov, Dmitry Lokshtanov, Segev Naveh-Tassa, Max Rose, Suhair Hanna, Calanit Raanan, Ori Brenner, Merav Kedmi, Hadas Keren-Shaul, Tsvee Lapidot, Itay Maza, Noa Novershtern, Jacob H. Flannel
Summary: Researchers have successfully generated synthetic whole embryo models with both embryonic and extraembryonic compartments using manipulated naive pluripotent stem cells. These models can complete embryonic development, develop organ progenitors, and exhibit complex structures similar to mouse embryos. This discovery highlights the potential of naive pluripotent cells.
Article
Cell Biology
Haibin Jiang, Mingxia Du, Yaning Li, Tengfei Zhou, Jia Lei, Hongqing Liang, Zhen Zhong, Rafia S. Al-Lamki, Ming Jiang, Jun Yang
Summary: It has been found that ID1 and ID3 play important roles in the survival and pluripotency of hESCs, regulating the transition between primed and naive-like states through the AKT signaling pathway. TCF3 mediates transcriptional inhibition of MCL1 to promote AKT phosphorylation.
CELL DEATH & DISEASE
(2022)
Review
Anatomy & Morphology
A. Thirumal Raj, Supriya Kheur, Ramesh Bhonde, Archana A. Gupta, Shankargouda Patil
Summary: This study assessed the effect of human MSC derived conditioned media on human cancer cell lines, identifying pro-carcinogenic and anti-carcinogenic molecules and effector pathways associated with different MSC types. The use of MSC-CM with pro-carcinogenic effect should be restricted in cancer patients regardless of the application nature.
Article
Multidisciplinary Sciences
Xin Huang, Kyoung-Mi Park, Paul Gontarz, Bo Zhang, Joshua Pan, Zachary McKenzie, Laura A. Fischer, Chen Dong, Sabine Dietmann, Xiaoyun Xing, Pavel Shliaha, Jihong Yang, Dan Li, Junjun Ding, Tenzin Lungjangwa, Maya Mitalipova, Shafqat A. Khan, Sumeth Imsoonthornruksa, Nick Jensen, Ting Wang, Cigall Kadoch, Rudolf Jaenisch, Jianlong Wang, Thorold W. Theunissen
Summary: This study mapped OCT4 protein interactions in naive and primed human ESCs, revealing specific interactions with BAF subunits that promote an open chromatin architecture at different gene sets. It highlights how a common transcription factor utilizes differential BAF complexes to control distinct transcriptional programs in naive and primed ESCs.
NATURE COMMUNICATIONS
(2021)
Article
Oncology
Joshua G. Dierolf, Andrew J. Watson, Dean H. Betts
Summary: Mouse embryonic stem cells and mouse epiblast stem cells represent two ends of the pluripotency continuum, naive and primed pluripotent states. They differ in growth factor requirements, DNA methylation patterns, and metabolic profiles, with metabolism now recognized as a driver of stem cell state and fate decisions. The muscle isoforms PKM1/2 play important roles in maintaining pluripotency and mediating the Warburg Effect, with potential implications for distinct pluripotency state maintenance.
EXPERIMENTAL CELL RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Andreas Lackner, Robert Sehlke, Marius Garmhausen, Giuliano Giuseppe Stirparo, Michelle Huth, Fabian Titz-Teixeira, Petra van Der Lelij, Julia Ramesmayer, Henry F. Thomas, Meryem Ralser, Laura Santini, Elena Galimberti, Mihail Sarov, A. Francis Stewart, Austin Smith, Andreas Beyer, Martin Leeb
Summary: Genetic screens and CRISPR/Cas9 gene disruption were used to study the regulatory circuits governing the exit from naive pluripotency in mammalian embryos. The transcriptome profiles of ESCs deficient for regulators revealed delays in the transition from naive to formative state, with gene networks conserved in primate embryos. Integrated analysis of mutant transcriptomes identified regulatory modules and the role of signalling pathways in directing this cell state transition.
Article
Reproductive Biology
Yao Xiao, Thiago F. Amaral, Pablo J. Ross, Delia A. Soto, Kenneth E. Diffenderfer, Aimee R. Pankonin, Surawich Jeensuk, Paula Tribulo, Peter J. Hansen
Summary: In cattle, inhibition of WNT signaling is crucial for the establishment of pluripotent embryonic stem cells, while JAK signaling pathway plays a role in maintaining pluripotency. IWR-1 can block the activation of STAT3, preventing differentiation of embryonic stem cells into a specific lineage.
BIOLOGY OF REPRODUCTION
(2021)
Article
Biochemistry & Molecular Biology
Irene Romayor, Lara Herrera, Maria Buron, Myriam Martin-Inaraja, Laura Prieto, Jone Etxaniz, Marta Ingles-Ferrandiz, Jose Ramon Pineda, Cristina Eguizabal
Summary: The study demonstrates that under naive culture NHSM medium conditions, human hiPSCs and hESCs exhibit higher viability and expression of naive pluripotency markers, and are more conducive to cell differentiation into endoderm and mesoderm.