Journal
IMMUNOLOGY LETTERS
Volume 161, Issue 1, Pages 133-137Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.imlet.2014.05.011
Keywords
Activation; Cell barcoding; Gamma-delta; Phosphoflow cytometry; T lymphocytes; Signal transduction
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Funding
- Institut National de la Sante et de la Recherche Medicale (INSERM)
- Universite Toulouse III: Paul Sabatier
- Institut Carnot Lymphome (CALYM)
- Laboratoire d'Excellence Toulouse Cancer (TOUCAN)
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Human gamma delta cells expressing TCRV gamma 9 are T lymphocytes with great potential for cancer immunotherapy and unconventional pattern of antigen specificity. These HLA-unrestricted lymphocytes are specifically reactive to non-peptide metabolites (phosphoantigens) and to the butyrophilin 3A (BTN3A/CD277) protein. Whether recognition of such highly different structures trigger the same activation signaling pathway remains unclear, however. Here we combined fluorescent cell barcoding and phosphoflow analysis of TCRV gamma 9(+) T lymphocytes to compare simultaneously the level of several signaling phosphoproteins after activation by phosphoantigen (BrHPP) or by anti-BTN3A (monoclonal antibody 20.1). This approach shows that the same pathways involving ZAP70, PLC gamma 2, Akt, NF kappa B p65, MAPK p38 and Erk1, were induced by either of these stimuli. These data strongly suggest the TCRV gamma 9(+) T lymphocytes detect phosphoantigens and butyrophilin A3 by the same recognition process.(C) 2014 Published by Elsevier B.V.
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