Journal
IMMUNOLOGY LETTERS
Volume 123, Issue 2, Pages 144-148Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.imlet.2009.03.005
Keywords
Barley-derived beta-D-glucans; Dectin-1; Signaling pathway; Structure
Categories
Funding
- Ministry of Education, Culture, Sports, Science, and Technology
- Japan Private School Promotion Foundation
Ask authors/readers for more resources
Barley-derived beta-glucan, a linear mixed-linkage beta-glucan composed of 1,3- and 1,4-beta-D-glucopyranose polymers, binds to dectin-1. However, whether it can trigger signal transduction via dectin-1 remains unclear. In this study, we used a reporter gene assay to determine whether barley-derived beta-D-glucan can activate NF-kappa B via dectin-1-mediated signaling when dectin-1 is cotransfected with Syk, CARD9, and Bcl10 in 293T cells. We found that barley-derived beta-D-glucan can activate NF-kappa B leading to cytokine production when dectin-1, Syk, CARD9, and Bcl10 are coexpressed in the cells. We also found that barley-derived beta-D-glucan can induce the phosphorylation of Syk and production of IL-6 in thioglycolate-elicited peritoneal macrophages. These results indicated that the immunostimulatory effects of barley-derived beta-D-glucan might be exerted, at least in part, via dectin-1. (C) 2009 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available