Article
Fisheries
Zhirong Fu, Srinivas Akula, Anna-Karin Olsson, Lars Hellman
Summary: In this study, we characterized the extended cleavage specificity and hematopoietic expression pattern of the chicken serine protease cathepsin G-like. Using substrate phage display and a panel of recombinant substrates, we identified a consensus sequence for this protease and found a preference for specific amino acids in the substrate. Immunohistochemical analysis confirmed the presence of cathepsin G-like in thrombocytes of chicken white blood cells.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2022)
Review
Immunology
Danyang Meng, Man Luo, Beibei Liu
Summary: CLEC-2, expressed on platelets and immune cells, plays a crucial role in infection and immunity, participating in processes such as thromboinflammation.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Immunology
Joshua H. Bourne, Nonantzin Beristain-Covarrubias, Malou Zuidscherwoude, Joana Campos, Ying Di, Evelyn Garlick, Martina Colicchia, Lauren Terry, Steven G. Thomas, Alexander Brill, Jagadeesh Bayry, Steve P. Watson, Julie Rayes
Summary: Platelet CLEC-2 reduces tissue inflammation by regulating inflammatory macrophage activation and trafficking, depending on the expression of its ligand podoplanin. This immune regulatory function is independent of platelet activation and secretion, and involves actin rearrangement, macrophage migration, and modulation of pro-inflammatory cytokines and immunosuppressive factors.
FRONTIERS IN IMMUNOLOGY
(2021)
Editorial Material
Hematology
Martha Sola-Visner
Summary: The study reveals a new role for PDPN in regulating embryonic megakaryocyte activation, and suggests a link between increased activation of eMk and midgestation brain hemorrhages in mice lacking PDPN.
Article
Immunology
Marzena Lenart, Magdalena Gorecka, Michal Bochenek, Emilia Barreto-Duran, Artur Szczepanski, Adrianna Galuszka-Bulaga, Natalia Mazur-Panasiuk, Kazimierz Weglarczyk, Andzelika Siwiec-Kozlik, Mariusz Korkosz, Pawel P. Labaj, Monika Baj-Krzyworzeka, Maciej Siedlar, Krzysztof Pyrc
Summary: In this study, it was found that SARS-CoV-2 infection inhibits NK cell functions through the LLT1-CD161 axis, leading to downregulation of NK cell phenotype and cytotoxicity. The expression of LLT1 protein is upregulated in infected epithelial cells and can be detected in serum of COVID-19 patients. Treatment with soluble LLT1 protein significantly reduces the proportion of CD161+ NK cells, impairs their ability to control SARS-CoV-2 infection, and inhibits granzyme B production and cytotoxicity, but not degranulation.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Hematology
Joshua H. Bourne, Christopher W. Smith, Natalie J. Jooss, Ying Di, Helena C. Brown, Samantha J. Montague, Mark R. Thomas, Natalie S. Poulter, Julie Rayes, Steve P. Watson
Summary: C-type lectin-like receptor 2 (CLEC-2) is highly expressed on platelets and plays a critical role in lymphatic development. This study demonstrates that CLEC-2 supports thrombus formation at sites of inflammation without affecting hemostasis. It suggests that CLEC-2 could be a promising therapeutic target for thromboinflammatory disorders.
THROMBOSIS AND HAEMOSTASIS
(2022)
Article
Immunology
Lucia La Sala, Sara Gandini, Antonino Bruno, Raffaele Allevi, Matteo Gallazzi, Pamela Senesi, Maria Teresa Palano, Paola Meregalli, Ermanno Longhi, Carmen Sommese, Livio Luzi, Emilio Trabucchi
Summary: This study assessed the effects of a two-dose regimen of the Pfizer mRNA vaccine on the immune system. The results showed that the vaccine stimulated the increase of B cells and NK cells, but had a limited effect on T cells. These findings raise concerns about the effectiveness of a two-dose vaccination in providing full immune protection.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Agriculture, Dairy & Animal Science
Samiru S. Wickramasuriya, Inkyung Park, Youngsub Lee, Luciana M. Richer, Chris Przybyszewski, Cyril G. Gay, Jolieke G. van Oosterwijk, Hyun S. Lillehoj
Summary: We investigated the effects of a higher dose of oral B. subtilis-cNK-2 treatment on coccidiosis, intestinal health, and gut microbiota composition in broiler chickens. The results showed that the NK treatment significantly improved growth performance, gut integrity, and mucosal immunity, while reducing fecal oocyst shedding. The gut microbiota composition in the NK group exhibited a distinct shift compared to the infected control group, indicating the restoration of dysbiosis caused by E. acervulina infection.
Editorial Material
Immunology
Kewreshini Naidoo, Marcus Altfeld
Summary: This study investigates the immune crosstalk between macrophages and NK cells in bronchioalveolar lavage fluid during SARS-CoV-2 infection in macaques, identifying the immunoregulatory properties of NK cells and their implications for viral persistence.
Article
Medicine, Research & Experimental
Chaojun Tang, Lei Wang, Yulan Sheng, Zhong Zheng, Zhanli Xie, Fan Wu, Tao You, Lijie Ren, Lijun Xia, Changgeng Ruan, Li Zhu
Summary: This study demonstrated that disturbed blood flow can induce platelet accumulation in atherosclerosis, with CLEC-2 gene knockout potentially reducing subendothelial accumulation of platelets and monocytes/macrophages, thereby ameliorating plaque formation.
Article
Veterinary Sciences
Samiru S. Wickramasuriya, Inkyung Park, Youngsub Lee, Woo H. Kim, Chris Przybyszewski, Cyril G. Gay, Jolieke G. van Oosterwijk, Hyun S. Lillehoj
Summary: Chicken NK-lysin peptide 2 (cNK-2) has direct cytotoxicity against apicomplexan parasites like Eimeria. Developing an effective oral delivery strategy for cNK-2 in the gut can reduce parasite fecundity and intestinal damage, while modulating gut immune responses. B. subtilis-cNK-2 treatment shows promise as an effective alternative to antibiotics in controlling coccidiosis by reducing parasite survival, body weight loss, and gut damage.
FRONTIERS IN VETERINARY SCIENCE
(2021)
Article
Cell Biology
Elizabeth J. Haining, Kate L. Lowe, Surasak Wichaiyo, Raghu P. Kataru, Zoltan Nagy, Dean Pj Kavanagh, Sian Lax, Ying Di, Bernhard Nieswandt, Benoit Ho-Tin-Noe, Babak J. Mehrara, Yotis A. Senis, Julie Rayes, Steve P. Watson
Summary: The study found that CLEC-2 expression on platelets is not necessary to maintain a barrier between the blood and lymphatic systems in unchallenged mice, but deficiency in CLEC-2 can lead to lymphatic vessel blood filling under conditions of chronic vascular remodeling. CLEC-2-driven platelet activation plays an essential role in vascular developmental programs.
Article
Biochemistry & Molecular Biology
Eva Persyn, Sigrid Wahlen, Laura Kiekens, Sylvie Taveirne, Wouter Van Loocke, Els Van Ammel, Filip Van Nieuwerburgh, Tom Taghon, Bart Vandekerckhove, Pieter Van Vlierberghe, Georges Leclercq
Summary: This study investigated the role of TXNIP in human NK cell differentiation and found that TXNIP promotes human NK cell differentiation by affecting protein synthesis and proliferation of early NK cell differentiation stages.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Letter
Oncology
Shoubao Ma, Michael A. Caligiuri, Jianhua Yu
Summary: This study identifies a four-stage developmental model for mouse NK cell development and defines two distinct populations of immature NK cells.
JOURNAL OF HEMATOLOGY & ONCOLOGY
(2022)
Article
Immunology
Hui Wang, Kangli Cao, Siyu Liu, Yuanhong Xu, Ling Tang
Summary: The study found that the frequency and absolute number of NK cells in patients with type 2 diabetes mellitus were significantly lower than in healthy individuals. The expression of surface receptors and cytokine production in NK cells were affected in patients, and correlated with high blood glucose levels.
FRONTIERS IN IMMUNOLOGY
(2022)