Article
Environmental Sciences
Elena Curto, Eder F. Mateus-Medina, Astrid Crespo-Lessmann, Ruben Osuna-Gomez, Cristina Ujaldon-Miro, Alba Garcia-Moral, Paula Galvan-Blasco, Lorena Soto-Retes, David Ramos-Barbon, Vicente Plaza
Summary: This study identified two subsets of eosinophils (E1 and E2) in induced sputum samples using flow cytometry, and found that their distribution and correlation varied among different types of asthma patients.
INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH
(2022)
Article
Chemistry, Analytical
Chengxin Wu, Xing Wei, Xue Men, Xuan Zhang, Yong-Liang Yu, Zhang-Run Xu, Ming-Li Chen, Jian-Hua Wang
Summary: CytoLM is a two-dimensional cytometry platform developed for single-particle and single-cell sampling and analysis, featuring high sensitivity and high temporal resolution. It combines VCCS-LIF and ICP-MS technologies to enable high-throughput, multi-dimensional, and multi-parameter characterization of particles and cells, showing vast potential in life science analysis.
ANALYTICAL CHEMISTRY
(2021)
Article
Multidisciplinary Sciences
Yang Wang, Li Feng Gu, Xincheng Zhao, Chengping Hu, Qiong Chen
Summary: This study investigated the association between TFR1 expression and asthma severity. The results showed that TFR1 expression was correlated with lung function, inflammatory macrophage phenotype, and inflammatory cytokines in asthma patients. These findings suggest that TFR1 may serve as a marker for indicating asthma severity.
Article
Chemistry, Multidisciplinary
M. Monsur Ali, Manali Mukherjee, Katherine Radford, Zil Patel, Alfredo Capretta, Parameswaran Nair, John D. D. Brennan
Summary: This study describes the first protein-binding allosteric RNA-cleaving DNAzyme (RCD) obtained by direct in vitro selection against eosinophil peroxidase (EPX). The RCD has strong affinity for EPX, shows high selectivity against related peroxidases and other eosinophil proteins, and is resistant to degradation by mammalian nucleases. An optimized RCD was used to develop both fluorescence and lateral flow assays, which demonstrated a clinical sensitivity of 100% and specificity of 96% when evaluated using patient sputum samples.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Chemistry, Analytical
Margarita Khariton, Conor J. McClune, Kara K. Brower, Sandy Klemm, Elizabeth S. Sattely, Polly M. Fordyce, Bo Wang
Summary: Microfluidic droplet assays enable single-cell PCR and sequencing analyses at unprecedented scales. Encapsulating cells within picoliter double emulsion allows sorting droplets with commercially available FACS machines, making it possible to isolate single cells based on phenotypes of interest. However, sorting DE droplets with standard cytometers requires small droplets that can pass FACS nozzles. Therefore, this study overcame limitations by using a plate-based RT-PCR assay to ameliorate the inhibition and demonstrated one-step RT-PCR from single cells in 20 pL DE droplets with fluorescence quantifiable via FACS.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Margarita Khariton, Conor J. McClune, Kara K. Brower, Sandy Klemm, Elizabeth S. Sattely, Polly M. Fordyce, Bo Wang
Summary: Microfluidic droplet assays enable single-cell PCR and sequencing analyses at unprecedented scales. However, sorting small droplets for FACS poses challenges for molecular biology. To overcome this, we used a plate-based RT-PCR assay and showed that heat lysis can alleviate inhibition for most cell lines, allowing RT-PCR to proceed in low-picoliter volumes.
ANALYTICAL CHEMISTRY
(2023)
Article
Allergy
Nami Shrestha Palikhe, Yingqi Wu, Emily Konrad, Vivek Dipak Gandhi, Brian H. Rowe, Harissios Vliagoftis, Lisa Cameron
Summary: This study found that during acute asthma exacerbations, the number of Th2 cells in peripheral blood correlated with ACQ6 and AQLQ scores, while ILC2 and eosinophil numbers did not. Subjects had higher CRTh2 expression during exacerbation compared to follow-up, and worse lung function. The decrease in CRTh2 expression during follow-up correlated with improvement in lung function.
Review
Biochemistry & Molecular Biology
Urszula Demkow, Anna Stelmaszczyk-Emmel
Summary: Extracellular vesicles (EVs) play a crucial role in respiratory diseases, but face obstacles in the analysis process, such as material collection, standardization of isolation techniques, and content analysis. Research focuses on the role of EVs in atopic respiratory diseases, exploring their clinical applicability as a diagnostic tool.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Allergy
Joost Brandsma, James P. R. Schofield, Xian Yang, Fabio Strazzeri, Clair Barber, Victoria M. Goss, Grielof Koster, Per S. Bakke, Massimo Caruso, Pascal Chanez, Sven-Erik Dahlen, Stephen J. Fowler, Ildiko Horvath, Norbert Krug, Paolo Montuschi, Marek Sanak, Thomas Sandstrom, Dominick E. Shaw, Kian Fan Chung, Florian Singer, Louise J. Fleming, Ian M. Adcock, Ioannis Pandis, Aruna T. Bansal, Julie Corfield, Ana R. Sousa, Peter J. Sterk, Ruben J. Sanchez-Garcia, Paul J. Skipp, Anthony D. Postle, Ratko Djukanovic
Summary: This study conducted a comprehensive analysis of the lipid composition in the induced sputum of asthma patients and healthy controls, revealing 9 different lipid phenotypes. The results indicated that abnormal lipid metabolism in asthma patients is associated with disease severity and lung function impairment.
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY
(2023)
Article
Respiratory System
Lijuan Du, Changyi Xu, Zhimin Zeng, Fengjia Chen, Kun Tang, Yuxia Liang, Yubiao Guo
Summary: BIRC3 expression is significantly increased in induced sputum of asthma and positively correlates with airway eosinophilic and peripheral blood allergic inflammation, type 2 cytokines, and airway obstruction. Increased BIRC3 might be involved in the pathogenesis of asthma.
BMC PULMONARY MEDICINE
(2022)
Review
Biochemical Research Methods
Kai Pang, Sihan Dong, Yuxi Zhu, Xi Zhu, Quanyu Zhou, Bobo Gu, Wei Jin, Rui Zhang, Yuting Fu, Bingchen Yu, Da Sun, Duanmu Zheng, Xunbin Wei
Summary: Flow cytometry is a versatile tool for detecting and measuring characteristics of cells or particles. Recent advancements in techniques such as in vivo photoacoustic FC, coherent Raman FC, and microfluidic FC have expanded the applications of FC in basic research and clinical practice.
JOURNAL OF BIOPHOTONICS
(2023)
Article
Immunology
A. Elisabeth Gressler, Sabrina Luebke, Bettina Wagner, Corinna Arnold, Katharina L. Lohmann, Christiane L. Schnabel
Summary: Equine asthma, a common disease affecting approximately 20% of horses, shows similarities to human asthma and is believed to be immune-mediated. By analyzing the cell populations in the lower airways of horses with different severities of equine asthma, researchers identified characteristic changes in the cell compositions, highlighting potential differences between severe cases and mild to moderate cases as well as healthy horses. These findings contribute to a better understanding of the pathogenesis of equine asthma.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Respiratory System
Madeleine E. Lemieux, Xavier T. Reveles, Jennifer Rebeles, Lydia H. Bederka, Patricia R. Araujo, Jamila R. Sanchez, Marcia Grayson, Shao-Chiang Lai, Louis R. DePalo, Sheila A. Habib, David G. Hill, Kathleen Lopez, Lara Patriquin, Robert Sussman, Roby P. Joyce, Vivienne I. Rebel
Summary: Researchers have developed a sputum-based test using flow cytometry and machine learning to assist physicians in diagnosing small to intermediate-sized lung nodules.
RESPIRATORY RESEARCH
(2023)
Article
Biochemical Research Methods
Jessie E. Arce, Joshua A. Welsh, Sean Cook, John Tigges, Ionita Ghiran, Jennifer C. Jones, Andrew Jackson, Matthew Roth, Aleksandar Milosavljevic
Summary: The NanoFlow Repository, the first implementation of the MIFlowCyt-EV framework, has been developed to meet the need for data sharing and minimum reporting standards for extracellular particles (EPs).
Article
Biochemical Research Methods
Paul H. Dannenberg, Jisoo Kang, Nicola Martino, Anokhi Kashiparekh, Sarah Forward, Jiamin Wu, Andreas C. Liapis, Jie Wang, Seok-Hyun Yun
Summary: This article demonstrates a cell barcode technology using laser particles, which can be used for cell sorting and routing through a microfluidic platform integrated with a high-speed spectrometer. By utilizing the spectral emission of laser particles, individual cells labeled with different laser colors can be distinguished and sorted.
Editorial Material
Respiratory System
Ivan Castellvi, Silvia Vidal, Claudia Valenzuela
ARCHIVOS DE BRONCONEUMOLOGIA
(2022)
Article
Respiratory System
Vicente Plaza, Isam Alobid, Cesareo Alvarez, Marina Blanco, Jorge Ferreira, Gabriel Garcia, Antonio Gomez-Outes, Fernando Gomez, Antonio Hidalgo, Javier Korta, Jesus Molina, Francisco Javier Pellegrini, Montserrat Perez, Javier Plaza, Manuel Praena, Santiago Quirce, Jose Sanz
Summary: In this study, the Spanish Asthma Management Guidelines (GEMA) have undergone a thorough review, leading to several significant changes. These changes include new FENO cut-off and severity classification in diagnosis, a more restrictive concept of intermittent asthma and expanded treatment options, as well as improvements in other aspects such as pediatric asthma scoring and the addition of new sections.
ARCHIVOS DE BRONCONEUMOLOGIA
(2022)
Article
Oncology
Carlos Zamora Atenza, Georgia Anguera, Mariona Riudavets Melia, Leticia Alserawan De Lamo, Ivana Sullivan, Andres Barba Joaquin, Jorgina Serra Lopez, M. Angels Ortiz, Maria Mulet, Silvia Vidal, Margarita Majem
Summary: This study analyzed the predictive effect of integrating systemic and tumor PD-L1 in patients with advanced NSCLC receiving PD-(L)1 blockade agents. The results showed that integrating circulating and tumor PD-L1 data can help in deciding the best treatment strategy.
CANCER IMMUNOLOGY IMMUNOTHERAPY
(2022)
Letter
Respiratory System
Alvar Agusti, Gaston De Stefano, Alberto Levi, Xavier Munoz, Christian Romero-Mesones, Oriol Sibila, Alejandra Lopez-Giraldo, Vicente Plaza Moral, Elena Curto, Andres L. Echazarreta, Silvana E. Marquez, Sergi Pascual-Guardia, Salud Santos, Alicia Marin, Luis Valdes, Fernando Saldarini, Clara Salgado, Georgina Casanovas, Sara Varea, Jose Rios, Rosa Faner
EUROPEAN RESPIRATORY JOURNAL
(2022)
Letter
Allergy
Leyre Baptista-Serna, Jose Manuel Rodrigo-Munoz, Pablo Minguez, Marcela Valverde-Monge, Ebymar Arismendi, Pilar Barranco, Blanca Barroso, Irina Bobolea, Jose Antonio Canas, Blanca Cardaba, Maria Jesus Cruz, Elena Curto, Javier Dominguez-Ortega, Raquel Garcia-Latorre, Francisco J. Gonzalez-Barcala, Carlos Martinez-Rivera, Joaquim Mullol, Xavier Munoz, Jose Maria Olaguibel, Cesar Picado, Vicente Plaza, Santiago Quirce, Manuel J. Rial, Beatriz Sastre, Lorena Soto, Antonio Valero, Victoria del Pozo, Joaquin Sastre
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY-IN PRACTICE
(2022)
Article
Allergy
Jennifer K. Quint, Sofie Arnetorp, Janwillem W. H. Kocks, Maciej Kupczyk, Javier Nuevo, Vicente Plaza, Claudia Cabrera, Chantal Raherison-Semjen, Brandie Walker, Erika Penz, Ileen Gilbert, Njira Lucia Lugogo, Ralf J. P. van der Valk
Summary: There is an association between SABA use and severe asthma exacerbations. Patients using 1 or 2 SABAs in the Netherlands, U.S. commercial, and U.S. Medicare datasets did not show an association with severe exacerbations, while this association was confirmed in the United Kingdom and Poland.
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY-IN PRACTICE
(2022)
Article
Allergy
Luis Perez de Llano, David Dacal Rivas, Nuria Marina Malanda, Vicente Plaza Moral, Jose Antonio Gullon Blanco, Mariana Munoz-Esquerre, Ismael Garcia-Moguel, Rocio M. Diaz Campos, Eva Martinez-Moragon, Alicia Harbenau Mena, Borja G. Cosio, Alicia Padilla Galo, Carolina Cisneros Serrano
Summary: This study compares the effectiveness of biologics in asthma-COPD overlap (ACO) and asthma patients. The results show that ACO patients treated with biologics have worse outcomes compared to asthma patients, with higher incidence of exacerbations and corticosteroid use, and lower percentage of controlled patients.
JOURNAL OF ASTHMA AND ALLERGY
(2022)
Article
Immunology
Ruben Osuna-Gomez, Silvia Barril, Maria Mulet, Carlos Zamora Atenza, Paloma Millan-Billi, Ana Pardessus, Douglas E. Brough, Helen Sabzevari, Roshanak T. Semnani, Diego Castillo, Silvia Vidal
Summary: This study aims to investigate the immune regulatory role of IL-35 in the development of fibrosis in interstitial lung disease (ILD). The results suggest that IL-35 may induce an anti-fibrotic response, regulating the effect of TGF-beta and the inflammatory response on CD4+ T cells. Furthermore, the TGF-beta: IL-35 ratio in BAL has been shown to be a potential biomarker to predict the outcome of F patients with ILD.
Article
Biochemistry & Molecular Biology
Marta Gil-Martinez, Clara Lorente-Sorolla, Jose M. Rodrigo-Munoz, Miguel Angel Lendinez, Gonzalo Nunez-Moreno, Lorena de la Fuente, Pablo Minguez, Ignacio Mahillo-Fernandez, Joaquin Sastre, Marcela Valverde-Monge, Santiago Quirce, Maria L. Caballero, Francisco J. Gonzalez-Barcala, Ebymar Arismendi, Irina Bobolea, Antonio Valero, Xavier Munoz, Maria Jesus Cruz, Carlos Martinez-Rivera, Vicente Plaza, Jose M. Olaguibel, Victoria del Pozo
Summary: MicroRNAs (miRNAs) are increasingly being used as biomarkers for diagnosis and treatment of various diseases. This study analyzed miRNA expression profiles in serum and lung tissue from severe asthma patients with and without oral corticosteroids (OCS) treatment. MiRNA-Seq and RT-qPCR were used for evaluation and validation of miRNA levels. Altered expression of miRNAs in serum and lung tissue was observed, with hsa-miR-221-5p and hsa-miR-769-5p showing the best differentiation between OCS-treated and untreated individuals. These miRNAs were found to be associated with important signaling pathways for asthma and regulated the expression of genes involved in these pathways. The findings suggest that miRNA profiles can serve as biomarkers for OCS treatment in asthma patients.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Allergy
Astrid Crespo-Lessmann, Elena Curto, Eder Freddy Mateus Medina, Esther Palones, Alicia Belda Soler, Soraya Sanchez Maza, Lorena Soto-Retes, Vicente Plaza
Summary: The purpose of this study was to determine the bronchial inflammatory phenotypes and associated characteristics in patients with asthma attending a specialist clinic. The study found that the most common phenotype was eosinophilic, and peripheral blood eosinophilia was the best predictor of bronchial eosinophilia. This information can help doctors select appropriate treatment and prevent asthma exacerbation.
JOURNAL OF ASTHMA AND ALLERGY
(2023)
Article
Biochemistry & Molecular Biology
Miriana d'Alessandro, Sara Gangi, Piera Soccio, Elisabet Canto, Ruben Osuna-Gomez, Laura Bergantini, Paolo Cameli, Gaia Fabbri, Sara Croce, Giulia Scioscia, Giusy Montuori, Matteo Fanetti, Giorgia Moriondo, Fabrizio Mezzasalma, Diego Castillo, Donato Lacedonia, Silvia Vidal, Elena Bargagli
Summary: This study investigated extracellular vesicle markers in bronchoalveolar lavage (BAL) samples from patients with interstitial lung diseases. It was found that these markers were related to fibrotic damage and were shared among different lung diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Chemistry, Medicinal
Noe Garin, Borja Zarate-Tamames, Laura Gras-Martin, Raimon Mila, Astrid Crespo-Lessmann, Elena Curto, Marta Hernandez, Conxita Mestres, Vicente Plaza
Summary: We conducted a systematic review and meta-analysis on electronic monitoring devices of inhalers (EMDs) in adult patients with asthma or COPD to examine their characteristics and clinical impact. Our search yielded eight observational studies and ten clinical trials, covering a wide range of clinical outcomes. The meta-analysis showed that adherence to inhalers improved in the EMD group over a three-month period. An exploratory analysis also found an improvement in ACT score. However, other clinical outcomes showed mixed results. Overall, EMDs have the potential to optimize adherence to inhaled therapy and may be of interest for other clinical outcomes.
Article
Gastroenterology & Hepatology
Luca Laghi, Eva Roman, Qiuyu Lan, Juan Camilo Nieto, Aleix Canalda-Baltrons, Maria Poca, Maria B. Sanchez-Rodriguez, Joan Claria, Edilmar Alvarado, Berta Cuyas, Elisabet Sanchez, Silvia Vidal, Carlos Guarner, Angels Escorsell, Chaysavanh Manichanh, German Soriano
Summary: In this study, we analyzed the blood metabolome of cirrhotic patients to investigate the potential mechanisms of a probiotic. We found that the multistrain probiotic may influence glutamine/glutamate metabolism, increasing the detoxification capacity of ammonia.
HEPATOLOGY COMMUNICATIONS
(2023)
Article
Chemistry, Medicinal
Vicente Plaza, Javier Dominguez-Ortega, Diego Gonzalez-Segura Alsina, Daniele Lo Re, Antoni Sicras-Mainar
Summary: Adding LAMA to LABA/ICS is recommended to improve control in patients with persistent asthma. This study found that triple therapy reduced exacerbations, improved lung function, and decreased healthcare costs compared to dual therapy, particularly in patients requiring systemic corticosteroids for severe exacerbations.
Article
Respiratory System
Javier Dominguez-Ortega, Vicente Plaza, Vicky W. Li, Eileen Fonseca, Luis Cea-Calvo, Ashley Martin, Marta Sanchez-Jareno, Joanne E. Brady, Jonathan Schelfhout
Summary: This study assessed the prevalence of chronic cough in Spanish adults and compared the characteristics between those with chronic cough and those without. The results showed that individuals with chronic cough had poorer health status, poorer mental health, higher healthcare utilization, and lower productivity at work and home compared to those without chronic cough.
CHRONIC RESPIRATORY DISEASE
(2022)