Article
Immunology
Lu Xia, Yu Liu, Zhiwei Zhang, Yajuan Gong, Tianyi Yu, Dan Zhao, Wen Qiu, Yingwei Wang, Jing Zhang
Summary: The expression of IL-6 in response to sublytic C5b-9 stimulation involves KLF4-mediated transactivation, PCAF-mediated acetylation of KLF4 and histones, and NF-kappa B activation.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Longfei Liu, Wen Ge, Zhiwei Zhang, Ya Li, Mengxiao Xie, Chenhui Zhao, Chunlei Yao, Can Luo, Zhijiao Wu, Wenbo Wang, Dan Zhao, Jing Zhang, Wen Qiu, Yingwei Wang
Summary: In this study, elevated levels of SOX9, GCN5, FGF1, and PDGF alpha were observed in the renal tissues of Thy-1N rats and GMCs stimulated with sublytic C5b-9. It was found that sublytic C5b-9 induced GMC proliferation by increasing the expression of SOX9, GCN5, FGF1, and PDGF alpha, and GCN5 mediated SOX9 acetylation to enhance FGF1 or PDGF alpha synthesis and GMC proliferation. Silencing renal SOX9, GCN5, FGF1, or PDGF alpha genes significantly reduced FGF1 and PDGF alpha expression, GMC proliferation, and urinary protein secretion in Thy-1N rats. Additionally, positive expression of these genes was also observed in the renal tissues of MsPGN patients.
Article
Medicine, Research & Experimental
Yuanyuan Li, Youfu Fang, Jing Liu
Summary: This study revealed the effects and mechanisms of TRPC6 on sublytic C5b-9-induced podocytes. Knockdown of TRPC6 reduced the damage to podocytes caused by sublytic C5b-9 by weakening the phosphorylation level of ERK1/2 to activate autophagy.
EXPERIMENTAL AND THERAPEUTIC MEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Wenbo Wang, Baomei Qian, Chenhui Zhao, Mingyu Peng, Longfei Liu, Mengxiao Xie, Na Peng, Qingling He, Shuai Ying, Yufeng Zhu, Tao Wang, Dajun Hu, Dan Zhao, Jing Zhang, Yingwei Wang, Wen Qiu
Summary: This study found that sublytic C5b-9 induces the production of CCL3/4 and accumulation of M phi through the PKC-alpha/p65/IRF-8 axis, aggravating the pathological changes of MsPGN.
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
(2022)
Article
Immunology
Shuai Ying, Longfei Liu, Can Luo, Yu Liu, Chenhui Zhao, Wen Ge, Ningxia Wu, Yuting Ruan, Weiming Wang, Jing Zhang, Wen Qiu, Yingwei Wang
Summary: This study found that KLF5 and TRAF6 play a promoting role in sublytic C5b-9-triggered TIMP3 gene transcription and expression, which provides a novel mechanistic insight into rat Thy-1N and human MsPGN.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2023)
Article
Medicine, General & Internal
Byung Chul Yu, Jin Hoon Park, Kyung Ho Lee, Young Seung Oh, Soo Jeong Choi, Jin Kuk Kim, Moo Yong Park
Summary: In this study, the potential of urinary C5b-9 as a prognostic biomarker for IgAN was evaluated. The results showed a positive correlation between baseline urinary C5b-9 levels and proteinuria, as well as a correlation between changes in urinary C5b-9 levels and changes in proteinuria and eGFR after treatment. However, baseline urinary C5b-9 levels were not a significant independent factor for predicting treatment response.
JOURNAL OF CLINICAL MEDICINE
(2022)
Article
Environmental Sciences
Zhibing Liu, Jinru Ma, Xulei Zuo, Xuesong Zhang, Yiting Hong, Shuyang Cai, Hua Huang, Feng Wang, Changhao Wu, Jiaxiang Zhang, Qixing Zhu
Summary: The study aims to explore renal tubular ferroptosis induced by trichloroethylene sensitization and its mechanism. Through observation of the kidneys of trichloroethylene-sensitized mice and HK-2 cells, it was found that C5b-9 and cytosolic Ca2+ overload may play a key role in renal tubular ferroptosis.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2022)
Article
Biochemistry & Molecular Biology
Rongrong Chai, Xueting Yu, Lian Li, Qiang Wei, Guangjun Jiao, Ying Li, Chen Yu, Yanling Mu, Qingqiang Yao, Yan Li, Fuwen Wang
Summary: In this study, a fusion protein was introduced and its mechanism to alleviate osteoarthritis (OA) was discussed. The targeted protein HB-NC4 showed better binding ability to chondrocytes and had a prolonged half-life period. HB-NC4 affected the levels of C3 and C5 and inhibited the formation of the membrane-attack complex, thereby affecting the expression of MAPK signalling pathway-related proteins to treat OA. This study provides a new method for OA therapy based on fusion proteins.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2023)
Article
Biochemistry & Molecular Biology
Kodai Hara, Asami Hishiki, Takako Hoshino, Kiho Nagata, Nao Iida, Yukimasa Sawada, Eiji Ohashi, Hiroshi Hashimoto
Summary: The RAD9-RAD1-HUS1 complex (9-1-1) plays a crucial role in DNA damage checkpoints in eukaryotes. Loading of 9-1-1 onto recessed DNA is facilitated by the RAD17 RFC-like complex (RAD17-RLC) and is necessary for activation of the ATR-CHK1 checkpoint pathway. The interaction between 9-1-1 and the protein RHINO, which contains a RAD1-binding motif, is shown to disturb the interaction between RAD17 and 9-1-1. These findings provide a deeper understanding of how RAD17-RLC recognizes 9-1-1 and suggest a functional role for RHINO in 9-1-1 loading/unloading and checkpoint activation.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2023)
Article
Biology
Maria G. Detsika, Elpida Diamanti, Kleio Ampelakiotou, Edison Jahaj, Stamatis Tsipilis, Nikolaos Athanasiou, Ioanna Dimopoulou, Stylianos E. Orfanos, Alexandra Tsirogianni, Anastasia Kotanidou
Summary: This study found that elevated levels of C3a and C5b-9 are associated with the severity and poor prognosis of COVID-19. C3a and C5b-9 can serve as predictive markers for the progression and outcome of COVID-19.
Article
Instruments & Instrumentation
Sunil Kumar Surapaneni, Nilkumar Patel, Li Sun, Nagavendra Kommineni, Anil Kumar Kalvala, Aragaw Gebeyehu, Peggy Arthur, Leanne C. Duke, Ramesh Nimma, David G. Meckes, Mandip Singh
Summary: This study explores the anti-cancer potential of synthetic cannabidiol (CBD) in triple negative breast cancer (TNBC) cells, and finds that CBD can increase sensitivity to chemotherapy drugs by inhibiting autophagy and reducing cell migration.
DRUG DELIVERY AND TRANSLATIONAL RESEARCH
(2022)
Article
Pharmacology & Pharmacy
Feng Wang, Meng Huang, Yican Wang, Yiting Hong, Dandan Zang, Chunjun Yang, Changhao Wu, Qixing Zhu
Summary: This study found that C5b-9 plays a crucial role in TCE-induced immune kidney damage, and its mechanism involves NLRP3-mediated pyroptosis (or inflammatory cell death).
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Immunology
Cathryn M. Kolka, Julie Webster, Ailin Lepletier, Clay Winterford, Ian Brown, Renee S. Richards, Wioleta M. Zelek, Yilang Cao, Ramlah Khamis, Karthik B. Shanmugasundaram, Alain Wuethrich, Matt Trau, Sandra Brosda, Andrew Barbour, Alok K. Shah, Guy D. Eslick, Nicholas J. Clemons, B. Paul Morgan, Michelle M. Hill
Summary: This study confirms the increased formation of C5b-9 in esophageal adenocarcinoma (EAC) and demonstrates the capability and heterogeneity in C5b-9 formation and shedding in Barrett's Esophagus (BE) and EAC cell lines following sublytic C9 exposure.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Pharmacology & Pharmacy
Asmaa I. Matouk, Mahmoud El-Daly, Heba A. Habib, Shaymaa Senousy, Sara Mohamed Naguib Abdel Hafez, AlShaimaa W. Kasem, Waleed Hassan Almalki, Abdulaziz Alzahrani, Ahmed Alshehri, Al-Shaimaa F. Ahmed
Summary: This study suggests that menthol has therapeutic potential against liver injury induced by sepsis. It reduces elevated liver enzymes and improves histopathological changes in the liver. It also decreases inflammation and oxidative stress levels and increases the levels of anti-apoptotic factors and biomarkers of regeneration and survival in the liver.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Cell Biology
Yueh-Lin Wu, Heng Lin, Hsiao-Fen Li, Ming-Jaw Don, Pei-Chih King, Hsi-Hsien Chen
Summary: Pharmacological studies have shown that Salvia miltiorrhiza extract (SME) and its derivative ST32db have beneficial effects in improving obesity-induced metabolic disorders. These compounds act through the ATF3-mediated C/EBPα and CHOP pathways to reduce obesity and diabetes in mice, suggesting their potential as therapeutic drugs for treating obesity-induced metabolic syndrome.