Journal
IET NANOBIOTECHNOLOGY
Volume 8, Issue 4, Pages 201-207Publisher
INST ENGINEERING TECHNOLOGY-IET
DOI: 10.1049/iet-nbt.2013.0035
Keywords
biodegradable materials; diseases; drug delivery systems; polymers; poly(vinyl alcohol)-coated chitosan microparticles; oral vaccine delivery system; hepatitis B vaccine; rat model; ionotropic gelation technique; loading capacity; loading efficiency; oral immunisation; intramuscular vaccination
Funding
- Chettinad Academy of Research and Education, Kelambakkam, Chennai, India
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The present study focused on the development of an effective oral vaccine delivery system of poly(vinyl alcohol)-coated chitosan microparticles-based recombinant hepatitis B vaccine. Chitosan microparticles were prepared by ionotropic gelation technique; they were loaded with recombinant hepatitis B vaccine and coated with poly(vinyl alcohol). The average sizes of the microparticles were measured in the range of 100-410 nm. The optimal loading capacity and loading efficiency were recorded around 3.4 and 74%, respectively. In vitro release study shows that the prepared microparticles release the antigen in a sustained manner. Moreover, the microparticles were resistant to simulated gastric environment and release the antigen in the targeted intestinal milieu. Furthermore, oral immunisation of rats with poly(vinyl alcohol)-coated chitosan hepatitis-B microparticles vaccine shows comparable seroprotective immune response to presently practiced intramuscular vaccination. The results demonstrated that poly(vinyl alcohol)-coated chitosan microparticles have the potential for being used as an oral vaccine delivery system for hepatitis B vaccine and may be a suitable alternative for needle-based vaccination.
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