Journal
IET NANOBIOTECHNOLOGY
Volume 8, Issue 4, Pages 295-303Publisher
INST ENGINEERING TECHNOLOGY-IET
DOI: 10.1049/iet-nbt.2013.0071
Keywords
proteins; molecular biophysics; gold; nanoparticles; nanomedicine; patient diagnosis; diseases; pH; ultraviolet spectra; visible spectra; circular dichroism; fluorescence; transmission electron microscopy; dissociation; biochemistry; gold nanoparticles; amyloid i(25-35)(2) peptide; aggregation; neurotoxicity; Alzheimer's disease detection; Ai(25a'35)(2) monomers; Ai(25a'35)(2) oligomers; Ai(25a'35)(2) fibrils; molar ratio; pH; ultraviolet-visible spectra; circular dichroism spectra; thioflavin T fluorescence assay; transmission electron microscope; dissociation; diagnostic tool; Au
Funding
- National Natural Science Foundation of China [81171453]
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Amyloid (25-35) (A(25-35)) peptide is a peculiar peptide for its rapid aggregation properties and high neurotoxicity in Alzheimer's disease. Here, the interactions between gold nanoparticles (GNPs) and A(25-35) monomers, oligomers and fibrils are explored under different molar ratio, temperature and pH by ultraviolet-visible and circular dichroism spectra, thioflavin T fluorescence assay and transmission electron microscope. It is concluded that A(25-35) can induce the aggregation of GNPs at certain concentration of A(25-35) monomer or oligomer. But at higher concentration of A(25-35), GNPs aggregates dissociate again. Furthermore, the aggregation rate increases at higher temperature or for lower pH. These results might provide the basis of a simple diagnostic tool for detecting Alzheimer's disease.
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