Journal
MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
Volume 57, Issue -, Pages 147-156Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.msec.2015.07.032
Keywords
Calcium oxalate monohydrate; Calcium oxalate dihydrate; Size effect; Cytotoxicity
Categories
Funding
- National Natural Science Foundation of China [21371077]
Ask authors/readers for more resources
Calcium oxalate crystals in urine often differ in size and crystal phase between healthy humans and patients with kidney stones. In this work, calcium oxalate monohydrate (COM) and dihydrate (COD) with sizes of about 50 nm, 100 nm, 1 mu m, 3 mu m, and 10 mu m were prepared by varying reactant concentration, reaction temperature, solvent mixing manner, and stirring speed. These crystals mainly had a smooth surface and no obvious pore structure, except COM-1 mu m. In cell culture medium, the zeta potential of crystals became increasingly negative with increasing size, and the absolute value of zeta potential of COD was greater than the same-sized COM. Results of cell viability and PI staining assays showed that the order of injury degree in African green monkey renal epithelial (Vero) cells caused by different sizes of COD was COD-50 nm > COD-100 nm > COD-1 mu m > COD-3 mu m > COD-10 mu m, and that of different sizes of COM was COM-1 mu m > COM-50 similar to COM-100 nm > COM-3 mu m > COM-10 mu m. COM-1 mu m presented the highest cytotoxicity in Vero cells, which was associated with its rougher surface, larger specific surface area (S-BET), and larger pore volume. Overall, these findings indicated that the physical properties of crystals play an important role in their cytotoxicity. (C) 2015 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available