Article
Computer Science, Hardware & Architecture
Naser Mohammadzadeh, Robert Wille, Oliver Keszocze
Summary: Digital microfluidics biochips require complex design processes, but recent advancements in one-pass design aim to address design gaps and inefficiencies. This article presents an efficient one-pass solution that is runtime efficient and outperforms previous heuristic approaches. Experimental results validate the benefits of this approach for large-scale assays.
ACM TRANSACTIONS ON DESIGN AUTOMATION OF ELECTRONIC SYSTEMS
(2021)
Article
Biotechnology & Applied Microbiology
Yuxin Wang, Yun-Sheng Chan, Matthew Chae, Donglu Shi, Chen-Yi Lee, Jiajie Diao
Summary: Biochips, a novel technology in biomolecular analysis, provide a compact and efficient solution for testing needs by integrating multiple functions in a single system. The pattern-control micro-electrode-dot-array (MEDA) design in biochips offers universality, replacing microchannels and enabling programmable control and dynamic grouping of electrodes for diverse testing requirements. This paper presents a visible biochip testing system that tracks the testing process in real time, and demonstrates its application in SARS-CoV-2 detection.
BIOENGINEERING-BASEL
(2023)
Article
Computer Science, Hardware & Architecture
Ikuru Yoshida, Shigeru Yamashita
Summary: Digital Microfluidic Biochips (DMFBs) have gained attention for their efficient execution of bio-chemical experiments. Sample preparation is crucial for generating sample droplets with desired concentration values in a DMFB. Uneven splitting of droplets can lead to unacceptable errors in concentration values. This paper proposes duplicating mixing nodes in a dilution graph to decrease the impact of errors caused by unbalanced splits. The presented experimental results demonstrate the potential of the proposed method.
IEICE TRANSACTIONS ON FUNDAMENTALS OF ELECTRONICS COMMUNICATIONS AND COMPUTER SCIENCES
(2023)
Article
Engineering, Electrical & Electronic
Sagarika Chowdhury, Piyali Datta, Rajat Kumar Pal, Goutam Saha
Summary: This paper presents an efficient fault detection mechanism for Digital Microfluidic Biochips (DMFBs) to identify faulty electrodes on a biochip array through test droplet routing and capacitive sensing. The proposed technique aims to ensure safe assay performance on a chip by identifying defective electrode positions or zones through boundary, row, and diagonal tests, even in complex scenarios. Evaluation of the approach considers various faulty environments.
IETE JOURNAL OF RESEARCH
(2021)
Article
Automation & Control Systems
Chen Jiang, Rong-Quan Yang, Bo Yuan
Summary: This paper proposes an evolutionary algorithm-based droplet routing method to minimize the arrival time of droplets. The method uses priority encoding and an improved decoding strategy to flexibly arrange the droplets and modifies the paths to satisfy fluidic constraints.
ENGINEERING APPLICATIONS OF ARTIFICIAL INTELLIGENCE
(2022)
Article
Computer Science, Hardware & Architecture
Debraj Kundu, Sudip Roy, Sukanta Bhattacharjee, Sohini Saha, Krishnendu Chakrabarty, Partha Pratim Chakrabarti, Bhargab B. Bhattacharya
Summary: Microfluidic biochips have shown great potential and versatility in automating biochemical protocols. Sample preparation, an essential part of these protocols, involves mixing fluids at a small scale. This article explores the impact of different mixing models on the dynamics of mixing steps and proposes factorization-based and volume-oriented dilution algorithms that outperform existing algorithms in terms of reactant cost, mixing time, and waste production for micro-electrode-dot-array biochips.
IEEE TRANSACTIONS ON COMPUTER-AIDED DESIGN OF INTEGRATED CIRCUITS AND SYSTEMS
(2022)
Article
Engineering, Multidisciplinary
Xijun Huang, Chuanpei Xu, Long Zhang, Cong Hu, Wei Mo
Summary: A novel test strategy is proposed for optimizing the test path of digital microfluidic biochips, which utilizes a hybrid algorithm and adaptive method to improve test efficiency and reduce test cost.
Review
Chemistry, Analytical
Ling Xia, Gongke Li
Summary: Sample preparation is a crucial step in complex sample analysis, as it affects the accuracy, selectivity, and sensitivity of analytical results. Conventional techniques often suffer from time-consuming and labor-intensive operations, which can be addressed by microfluidic sample preparation methods. This review provides an overview of the progress in microfluidic sample preparation techniques over the past 3 years, highlighting the implementation of typical methods in microfluidic formats. The challenges and future prospects of microfluidic sample preparation techniques are also discussed.
JOURNAL OF SEPARATION SCIENCE
(2023)
Article
Biochemical Research Methods
Nicole Jagnandan, Jose Morachis
Summary: Single-cell RNA-Sequencing has enabled the detection of rare cell populations, microbial populations, and cancer mutations. The quality of single-cell transcriptomics relies on effective sample preparation and cell sorting techniques that preserve RNA quality and remove dead cells or debris. The WOLF(R) Cell Sorter and WOLF G2(R) are microfluidic-based cell sorters that offer gentle sorting technology compatible with various RNA-sequencing platforms, improving data per cell and library complexity.
Review
Biochemical Research Methods
Ryan Zenhausern, Chia-Hung Chen, Jeong-Yeol Yoon
Summary: The techniques for preparing clinical samples have been perfected for diagnostic testing, but microfluidics has emerged as a popular method for rapid virus detection in resource-limited environments. Early microfluidic prototypes faced limitations due to reliance on off-chip preprocessing steps and external equipment, but recent advancements have allowed for all-in-one, automated virus detection with no need for specialized training or complex machinery. These devices can operate independently and efficiently to provide rapid, automated sample preparation and efficient detection of viral samples.
Article
Biochemical Research Methods
Tengbao Xie, Ping Wang, Lei Wu, Bangyong Sun, Qiang Zhao, Gang Li
Summary: The study introduces a simple system for sample discretization, using water-soluble film and a syringe to rapidly partition samples, which can be applied in digital PCR and other fields such as single-cell analysis and diagnostics.
Article
Engineering, Electrical & Electronic
Yun-Sheng Chan, Chen-Yi Lee
Summary: The digital lab-on-CMOS chip for nucleic acid amplification test features novel circuit design and precise microfluidic operations, enabling it to meet various medical testing requirements while reducing reagent consumption and test time/cost.
IEEE TRANSACTIONS ON CIRCUITS AND SYSTEMS II-EXPRESS BRIEFS
(2022)
Article
Computer Science, Hardware & Architecture
Sudip Poddar, Gerold Fink, Werner Haselmayr, Robert Wille
Summary: Sample preparation is a crucial task in medical applications, with microfluidic devices offering solutions for this purpose. A generic approach has been proposed to address the diversity of different microfluidic platforms, enabling platform-independent sample preparation.
IEEE TRANSACTIONS ON COMPUTER-AIDED DESIGN OF INTEGRATED CIRCUITS AND SYSTEMS
(2022)
Article
Green & Sustainable Science & Technology
Xianhai Meng, Simran Das, Junyu Meng
Summary: Construction, as a major industry sector, is gradually transitioning from linear to circular economy, but faces slow progress due to various barriers. Digital technologies, such as the digital twin, have the potential to alleviate these barriers and challenges. This study examines the implementation of circular economy and the integration of digital twin in construction through a combination of expert interviews and questionnaire surveys. The findings suggest that design and demolition, closely linked to circular economy strategies, are crucial, while the digital twin can significantly improve circular economy practice throughout different project phases.
Article
Chemistry, Analytical
Danielly S. Rocha, Richard P. S. de Campos, Habdias A. Silva-Neto, Gerson F. Duarte-Junior, Fethi Bedioui, Wendell K. T. Coltro
Summary: Digital microfluidics (DMF) is a versatile lab-on-a-chip platform integrated with UV-LEDs for sample degradation and colorimetric detection, as demonstrated in the analysis of S-nitrosocysteine (CySNO). The proposed integration showed satisfactory correlation with the results from a desktop scanner, and the analytical parameters revealed linear behavior in the CySNO concentration range of 12.5-400 mu mol L-1, with a limit of detection of 2.8 mu mol L-1. Synthetic serum and human plasma samples were successfully analyzed with no statistical difference observed.
ANALYTICA CHIMICA ACTA
(2023)
Article
Computer Science, Hardware & Architecture
Yi-Hang Chen, Jian-Yu Chen, Juinn-Dar Huang
ACM JOURNAL ON EMERGING TECHNOLOGIES IN COMPUTING SYSTEMS
(2016)
Article
Computer Science, Hardware & Architecture
Sukanta Bhattacharjee, Sudip Poddar, Sudip Roy, Juinn-Dar Huang, Bhargab B. Bhattacharya
IEEE TRANSACTIONS ON COMPUTER-AIDED DESIGN OF INTEGRATED CIRCUITS AND SYSTEMS
(2017)
Article
Computer Science, Hardware & Architecture
Sukanta Bhattacharjee, Yi-Ling Chen, Juinn-Dar Huang, Bhargab B. Bhattacharya
ACM TRANSACTIONS ON EMBEDDED COMPUTING SYSTEMS
(2018)
Article
Computer Science, Hardware & Architecture
Ankur Gupta, Juinn-Dar Huang, Shigeru Yamashita, Sudip Roy
ACM TRANSACTIONS ON DESIGN AUTOMATION OF ELECTRONIC SYSTEMS
(2019)
Article
Computer Science, Hardware & Architecture
Sukanta Bhattacharjee, Robert Wille, Juinn-Dar Huang, Bhargab B. Bhattacharya
IEEE TRANSACTIONS ON COMPUTER-AIDED DESIGN OF INTEGRATED CIRCUITS AND SYSTEMS
(2020)
Proceedings Paper
Computer Science, Hardware & Architecture
Ling-Yen Song, Yi-Ling Chen, Yung-Chun Lei, Juinn-Dar Huang
2019 IEEE 37TH INTERNATIONAL CONFERENCE ON COMPUTER DESIGN (ICCD 2019)
(2019)
Proceedings Paper
Computer Science, Hardware & Architecture
Ling-Yen Song, Yu-Ying Li, Yung-Chun Lei, Juinn-Dar Huang
2019 IEEE COMPUTER SOCIETY ANNUAL SYMPOSIUM ON VLSI (ISVLSI 2019)
(2019)
Proceedings Paper
Engineering, Electrical & Electronic
Kang-Yi Fan, Shigcru Yamashita, Juinn-Dar Huang
2019 INTERNATIONAL SYMPOSIUM ON VLSI DESIGN, AUTOMATION AND TEST (VLSI-DAT)
(2019)
Proceedings Paper
Engineering, Electrical & Electronic
Juinn-Dar Huang, Chia-Hung Liu, Wei-Hao Yang
PROCEEDINGS OF THE 2018 26TH IFIP/IEEE INTERNATIONAL CONFERENCE ON VERY LARGE SCALE INTEGRATION (VLSI-SOC)
(2018)
Proceedings Paper
Computer Science, Hardware & Architecture
Chia-Cheng Wu, Kung-Han Ho, Juinn-Dar Huang, Chun-Yao Wang
2018 IEEE COMPUTER SOCIETY ANNUAL SYMPOSIUM ON VLSI (ISVLSI)
(2018)
Proceedings Paper
Computer Science, Hardware & Architecture
Yung-Chun Lei, Tien-Kuo Lin, Juinn-Dar Huang
2018 IEEE COMPUTER SOCIETY ANNUAL SYMPOSIUM ON VLSI (ISVLSI)
(2018)
Proceedings Paper
Automation & Control Systems
Sukanta Bhattacharjee, Robert Wille, Juinn-Dar Huang, Bhargab B. Bhattacharya
PROCEEDINGS OF THE 2018 DESIGN, AUTOMATION & TEST IN EUROPE CONFERENCE & EXHIBITION (DATE)
(2018)
Proceedings Paper
Engineering, Electrical & Electronic
Juinn-Dar Huang, Yi-Hang Chen, Jia-Shin Lu
2017 IFIP/IEEE INTERNATIONAL CONFERENCE ON VERY LARGE SCALE INTEGRATION (VLSI-SOC)
(2017)
Proceedings Paper
Engineering, Electrical & Electronic
Yung-Chun Lei, Tung-Hsuan Lin, Juinn-Dar Huang
2016 29TH IEEE INTERNATIONAL SYSTEM-ON-CHIP CONFERENCE (SOCC)
(2016)
Proceedings Paper
Computer Science, Information Systems
Yung-Chun Lei, Yi-Ling Chen, Juinn-Dar Huang
PROCEEDINGS OF 2016 IEEE BIOMEDICAL CIRCUITS AND SYSTEMS CONFERENCE (BIOCAS)
(2016)