Tailored Fringed Platforms Produced by Laser Interference for Aligned Neural Cell Growth

Ordering neural cells is of interest for the development of neural interfaces. The aim of this work is to demonstrate an easy-to-use, versatile, and cost/time effective laser-based approach for producing platforms that promote oriented neural growth. We use laser interferometry to generate fringed channels with topography on partially reduced graphene oxide layers as a proof-of-concept substrate. We study cell adhesion, morphology, viability, and differentiation in cultures of embryonic neural progenitor cells on platforms with a 9.4m period. Results evidence that fringed platforms significantly promote neurite alignment (approximate to 50% at 6d), while preserving viability and neural differentiation.