Journal
LWT-FOOD SCIENCE AND TECHNOLOGY
Volume 60, Issue 2, Pages 672-677Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.lwt.2014.09.028
Keywords
Pulsed electric field; Saccharomyces cerevisiae; Sublethally; Sterilization; Selective culture medium
Categories
Funding
- Chinese National 863 project [2011AA100801]
- Chinese National Science Fund [21376094, 31301559]
- S&T project of Guangdong Province [2012A0 20200002, 2012A020100005]
- Guangdong Province Government (China)
Ask authors/readers for more resources
The survival states of Saccharomyces cerevisiae cells after being treated by pulsed electric field (PEF), intact, sublethally injured or dead, were quantitatively investigated in this paper. A selective culture medium with critical NaCl concentration of 4.25% (w/v) was developed firstly and then used to identify and quantify the sublethally injured cells (SICs) from dead and intact cells. Results showed that both the PEF intensity and the total treatment time affected the percent of SICs significantly. About 90% of the cells were sublethally injured when they were exposed to PEF with electric field strength of 5 kV/cm and total treatment time of 1.2 ms, which implied that a lower electric field strength (e.g. 5-10 kV/cm) or a shorter treatment time would produce a great number of SICs. And while under more severe circumstances, more and more microorganisms had been killed. In addition, the biological characteristics and potential value of such sublethally injured cells are also discussed. (C) 2014 Elsevier Ltd. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available