Article
Biochemistry & Molecular Biology
Min Liu, Yuan Meng, Leisheng Zhang, Zhongchao Han, Xiaoming Feng
Summary: Study found that the combined use of IL-2, IL-15, IL-18 can best promote the ex vivo expansion and proportion of NK cells in peripheral blood mononuclear cells. Meanwhile, the obtained NK cell population expressed high levels of activating molecules (CD16 and NKG2D) and exhibited splendid cytotoxicity against K562 cell line.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Review
Biochemistry & Molecular Biology
Janneke Hoogstad-van Evert, Romy Paap, Annemiek Nap, Renate van der Molen
Summary: Endometriosis is a common gynecological disease that greatly affects the reproductive health and quality of life of patients. Current treatment options have limitations, necessitating the development of new therapeutic strategies. NK cell therapy has shown promise as a potential treatment, but further research is needed to determine its safety and efficacy.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biology
Aviad Ben-Shmuel, Batel Sabag, Abhishek Puthenveetil, Guy Biber, Moria Levy, Tammir Jubany, Fatima Awwad, Roshan Kumar Roy, Noah Joseph, Omri Matalon, Jessica Kivelevitz, Mira Barda-Saad, Stipan Jonjic
Summary: NK cells play a crucial role in immunity, and the mechanisms behind their discrimination between healthy and cancer cells are not fully understood. This study reveals that phosphorylation of SHP-1 by PKC-theta promotes the inhibition of NK cell activation, and silencing PKC-theta reduces NK cell activation and cytotoxicity.
Article
Immunology
Linlin Wang, Longfei Li, Yuye Li, Chunyu Huang, Ruochun Lian, Tonghua Wu, Jingwen Ma, Yan Zhang, Yanxiang Cheng, Lianghui Diao, Yong Zeng
Summary: This study found that women with endometriosis may have decreased peripheral NK cytotoxicity and increased uterine macrophages, especially in mixed endometriosis patients. The findings suggest that NK cytotoxicity and endometrial immune cell profiles could be useful for diagnosing and treating endometriosis and related reproductive diseases.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Biochemical Research Methods
Sagar N. Agnihotri, Giovanni Stefano Ugolini, Matthew Ryan Sullivan, Yichao Yang, Agustin De Ganzo, Ji Won Lim, Tania Konry
Summary: Most common cellular analysis methods employ a top-down approach, which destroys the cell and prevents correlation between genomics and functional assays. This study presents a bottom-up approach using single-cell tools, allowing functional phenotyping through observing cytotoxicity and probing underlying biology.
Article
Immunology
Lukman O. Afolabi, Jiacheng Bi, Liang Chen, Xiaochun Wan
Summary: The study demonstrated that Piperlongumine (PL), a natural alkaloid obtained from long pepper fruit, has both antitumor and immunomodulatory potential on all tested tumors, increasing tumor susceptibility to NK cell cytolysis. This suggests that PL could be explored as a complementary approach to NK cell immunotherapy for cancer treatment.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2021)
Article
Oncology
Xian Lu, Chun Xu, Jie Dong, Shuguang Zuo, Hailin Zhang, Chunping Jiang, Junhua Wu, Jiwu Wei
Summary: The study revealed that liraglutide can enhance antitumor immune responses in hepatocellular carcinoma patients by improving NK-mediated cytotoxicity and suppressing the IL-6/STAT3 signaling pathway, indicating a novel therapeutic role of liraglutide in cancer therapy through manipulation of innate immunity.
TRANSLATIONAL ONCOLOGY
(2021)
Article
Oncology
Batel Sabag, Moria Levy, Jessica Kivelevitz, Nataly Dashevsky, Aviad Ben-Shmuel, Abhishek Puthenveetil, Fatima Awwad, Mira Barda-Saad
Summary: This study found that mechanotransduction plays a role in regulating signaling and activation versus inhibition balance in NK cells through the control of actin movement by the cytoskeletal component WASp. Understanding the interaction between NK cells and the tumor microenvironment is crucial for the development of immunotherapy.
Article
Microbiology
Sho Sugawara, Brady Hueber, Griffin Woolley, Karen Terry, Kyle Kroll, Cordelia Manickam, Daniel R. Ram, Lishomwa C. Ndhlovu, Paul Goepfert, Stephanie Jost, R. Keith Reeves
Summary: Natural killer (NK) cells play important roles in defense against HIV-1 and SIV infections, but their functions are often dysregulated. The comprehensive understanding of NK cell signaling is lacking. In this study, we developed a multiplex signaling assay for NK cells and found that CD16 signaling is downregulated in chronic lentiviral infections. We demonstrated that increased ADAM17 expression and elevated plasma IL-18 levels are involved in this downregulation, and perturbing ADAM17 can restore CD16 expression and signaling. This study provides insights into impaired NK cell activities and offers potential for NK cell-based immunotherapeutics for HIV-1 disease.
Article
Multidisciplinary Sciences
Jia-Yuan Zhang, Fiona Hamey, Dominik Trzupek, Marius Mickunas, Mercede Lee, Leila Godfrey, Jennie H. M. Yang, Marcin L. Pekalski, Jane Kennet, Frank Waldron-Lynch, Mark L. Evans, Timothy I. M. Tree, Linda S. Wicker, John A. Todd, Ricardo C. Ferreira
Summary: Low-dose interleukin-2 (IL-2) has shown promise in treating type 1 diabetes, and this study investigates the cellular and transcriptional changes in patients receiving low-dose IL-2 therapy. The results show an anti-inflammatory gene expression signature that persists for at least a month after treatment, indicating the potential long-term clinical benefits of IL-2 immunotherapy.
NATURE COMMUNICATIONS
(2022)
Article
Oncology
Rui Zhang, Qingxi Liu, Sa Zhou, Hongpeng He, Mingfeng Zhao, Wenjian Ma
Summary: In this study, a genetically modified bifunctional CAR-NK cell therapy was developed for the treatment of acute myeloid leukemia (AML). The bifunctional CD33/B16 CAR-NK cells showed superior killing efficiency towards AML cells compared to CAR-NK cells targeting CD33 only. In vivo studies also demonstrated effective clearance of leukemic cells and improved survival. These findings suggest a promising CAR-NK approach for AML treatment and potentially other tumors.
Article
Oncology
David B. Rosen, Anne Mansson Kvarnhammar, Burkhardt Laufer, Thomas Knappe, Jens Jakob Karlsson, Enping Hong, Yu-Chi Lee, Dhruv Thakar, Luis Alejandro Zuniga, Kathy Bang, Simran Singh Sabharwal, Karan Uppal, Janne Damm Olling, Kristian Kjaergaard, Thomas Kurpiers, Meike Schnabel, Diana Reich, Philipp Glock, Joachim Zettler, Mathias Krusch, Ana Bernhard, Stefan Heinig, Valentino Konjik, Thomas Wegge, Yvonne Hehn, Steffen Killian, Laura Viet, Josefine Runz, Frank Faltinger, Mohammad Tabrizi, Kristin Laura Abel, Vibeke Miller Breinholt, Stina M. Singel, Kennett Sprogoe, Juha Punnonen
Summary: Recombinant interleukin-2 (IL-2) is an approved cancer immunotherapy, but it has severe toxicities and suboptimal pharmacokinetics. TransCon IL-2 beta/gamma is a novel prodrug designed to address these issues, showing sustained release of IL-2 and potential antitumor activity in animal models.
JOURNAL FOR IMMUNOTHERAPY OF CANCER
(2022)
Article
Oncology
Malena Bodden, Aline Haecker, Jasmin Roeder, Anne Kiefer, Congcong Zhang, Anita Bhatti, Jordi Pfeifer Serrahima, Evelyn Ullrich, Ines Kuehnel, Winfried S. Wels
Summary: In contrast to T lymphocytes, natural killer (NK) cells do not require prior sensitization but are rapidly activated upon encountering virally infected or neoplastic cells. In addition, NK cells can be safely applied in an allogeneic setting, making them important effector cells for the development of off-the-shelf therapeutics for adoptive cancer immunotherapy.
Review
Immunology
Hyoungjun Ham, Michael Medlyn, Daniel D. Billadeau
Summary: NK cell-mediated cytotoxicity is essential for protection from infections and cancer, involving lytic granule development and degranulation processes. Studies on molecular regulations of NK cell cytotoxicity are providing insights into improving anti-cancer therapies and discovering new therapeutic opportunities.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Immunology
Chao Niu, Min Li, Yongchong Chen, Xiaoying Zhang, Shan Zhu, Xin Zhou, Lei Zhou, Zhaozhi Li, Jianting Xu, Ji-Fan Hu, Yufeng Wang, Jiuwei Cui
Summary: A novel lncRNA, called NCAL1, was discovered to enhance the cytotoxicity of NK cells by activating Gab2 protein. NCAL1 upregulated Gab2 expression epigenetically, thus promoting NK cell cytotoxicity and potentially serving as a therapeutic target for improving NK cell therapy.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Allergy
Sang-Hwan Seo, Min Seong Jang, Doo-Jin Kim, Seok-Min Kim, Se-Chan Oh, Cho-Rok Jung, Yunji Park, Sang-Jun Ha, Haiyoung Jung, Young-Jun Park, Suk Ran Yoon, Inpyo Choi, Tae-Don Kim
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY
(2018)
Article
Immunology
Arum Park, Yunhee Lee, Mi Sun Kim, Young Ju Kang, Young-Jun Park, Haiyoung Jung, Tae-Don Kim, Hee Gu Lee, Inpyo Choi, Suk Ran Yoon
FRONTIERS IN IMMUNOLOGY
(2018)
Article
Biochemistry & Molecular Biology
Hangsak Huy, Tae-Don Kim, Won Sam Kim, Dong Oh Kim, Jae-Eun Byun, Mi Jeong Kim, Young-Jun Park, Suk Ran Yoon, Ji-Yoon Noh, Jungwoon Lee, Kyoo-Hyung Lee, Inpyo Choi, Haiyoung Jung
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2018)
Review
Biochemistry & Molecular Biology
Ling-Zu Kong, Nisansala Chandimali, Ying-Hao Han, Dong-Ho Lee, Ji-Su Kim, Sun-Uk Kim, Tae-Don Kim, Dong Kee Jeong, Hu-Nan Sun, Dong Sun Lee, Taeho Kwon
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2019)
Article
Medicine, General & Internal
Arum Park, Yunjeong Yang, Yunhee Lee, Mi Sun Kim, Young-Jun Park, Haiyoung Jung, Tae-Don Kim, Hee Gu Lee, Inpyo Choi, Suk Ran Yoon
JOURNAL OF CLINICAL MEDICINE
(2019)
Article
Immunology
Sung-Jin Yoon, Dong Hyun Jo, Seung-Ho Park, Jun-Young Park, Yoo-Kyung Lee, Moo-Seung Lee, Jeong-Ki Min, Haiyoung Jung, Tae-Don Kim, Suk Ran Yoon, Su Wol Chung, Jeong Hun Kim, Inpyo Choi, Young-Jun Park
FRONTIERS IN IMMUNOLOGY
(2019)
Review
Immunology
Ji-Yoon Noh, Suk Ran Yoon, Tae-Don Kim, Inpyo Choi, Haiyoung Jung
JOURNAL OF IMMUNOLOGY RESEARCH
(2020)
Article
Engineering, Biomedical
Young Ha Ahn, Long Ren, Seok Min Kim, Sang-Hwan Seo, Cho-Rok Jung, Da Seul Kim, Ji-Yoon Noh, Soo Yun Lee, Hyunseung Lee, Mi Young Cho, Haiyoung Jung, Suk Ran Yoon, Jung-Eun Kim, Sang Nam Lee, Sohyun Kim, Il Woo Shin, Hong Sik Shin, Kwan Soo Hong, Yong Taik Lim, Inpyo Choi, Tae-Don Kim
Article
Andrology
Arum Park, Yunjeong Yang, Junyoung Jo, Suk Ran Yoon
Summary: This study investigated the therapeutic effect of various MYOMI formulations with reduced constituents on cyclophosphamide-induced male infertility using a mouse model. The results showed that MYOMI formulations could restore cyclophosphamide-induced dysfunction, reduce apoptosis of germ cells and oxidative stress, and improve spermatogenesis. Among the formulations, MYOMI-7 showed better results in terms of recovering testis damage and improving fertility.
Review
Biochemistry & Molecular Biology
Young Ha Ahn, Se-Chan Oh, Shengtao Zhou, Tae-Don Kim
Summary: Tryptophanyl-tRNA synthetase (WRS) is an essential enzyme in translation and has potential pharmacological implications in diseases. Despite being primarily viewed as a Trp-producing enzyme, studies are emerging on WRS as a potential therapeutic target for drug development.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Immunology
Yunhee Lee, Arum Park, Young-Jun Park, Haiyoung Jung, Tae-Don Kim, Ji-Yoon Noh, Inpyo Choi, Seungjin Lee, Suk Ran Yoon
Summary: G-Rg3 promotes NK cell cytotoxicity and activates the MAPK/ERK pathway. It increases the expression of NK-activating receptors and cytolytic molecules, while not affecting NK cell differentiation.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Chunli Wang, Lingzu Kong, Seokmin Kim, Sunyoung Lee, Sechan Oh, Seona Jo, Inhwan Jang, Tae-Don Kim
Summary: This review summarizes the roles of IL-7 and IL-7R alpha in immunity and cancer, and discusses their recent advances and potential as immunotherapy tools.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Medicine, General & Internal
Arum Park, Yunjeong Yang, Yunhee Lee, Haiyoung Jung, Tae-Don Kim, Ji-Yoon Noh, Seungjin Lee, Suk Ran Yoon
Summary: Aurantii Fructus Immaturus (AFI), a traditional herbal medicine, has diverse physiological effects and can enhance NK cell activity through the activation of the ERK signaling pathway. Additionally, AFI also inhibits cancer metastasis.
FRONTIERS IN MEDICINE
(2022)
Article
Immunology
Young-Ju Kang, Hee Jun Cho, Yunhee Lee, Arum Park, Mi Jeong Kim, In Cheul Jeung, Yong-Wook Jung, Haiyoung Jung, Inpyo Choi, Hee Gu Lee, Suk Ran Yoon
Summary: In this study, the immune status in endometriosis development was investigated. Increased Th17 cell population and IL-17A level were found in the peritoneal fluid of endometriosis patients. IL-17A was shown to enhance endometrial cell survival, inhibit NK cell mediated cytotoxicity, induce HLA-G expression, and promote migration of endometrial cells. These findings suggest that targeting IL-17A could be a potential strategy for endometriosis treatment.