Article
Agriculture, Dairy & Animal Science
Vinod Kumar Gupta, S. K. Srivastava, S. K. Ghosh, N. Srivastava, G. Singh, M. R. Verma, Rahul Katiyar, Ramamoorthy Muthu, L. Bhutia, Abhishek Kumar, Rohit Singh
Summary: The study found that good-quality ejaculates had significantly higher levels of Testosterone and PGE(2), while poor-quality ejaculates had higher levels of estradiol, progesterone, oxidative stress, and ASAs. It can be concluded that oxidative stress and hormonal imbalance may lead to an increase in dead and defective sperm, ultimately resulting in poor semen quality ejaculates.
ANIMAL BIOTECHNOLOGY
(2022)
Article
Biology
Maria J. Lorite, Ariana Casas-Roman, Lourdes Girard, Sergio Encarnacion, Natalia Diaz-Garrido, Josefa Badia, Laura Baldoma, Daniel Perez-Mendoza, Juan Sanjuan
Summary: The second messenger cyclic diguanylate (cdG) is known to play a crucial role in biofilm formation in bacteria. This study found that high levels of cdG not only promote the secretion of proteins involved in adhesion and biofilm formation, but also facilitate the export of cytoplasmic proteins. These cytoplasmic proteins have been previously described as moonlighting or multifunctional proteins, often found extracellularly or at the bacterial cell surface. The findings suggest that cdG promotes the active exportation of cytoplasmic proteins through yet unknown mechanisms involving post-translational modification.
Article
Multidisciplinary Sciences
Yoshitaka Fujihara, Sarah Herberg, Andreas Blaha, Karin Panser, Kiyonori Kobayashi, Tamara Larasati, Maria Novatchkova, Hans-Christian Theussl, Olga Olszanska, Masahito Ikawa, Andrea Pauli
Summary: Fertilization is a crucial process initiating new individual development in sexually reproducing species. SPACA4, a sperm protein, is essential for efficient fertilization in mammals like mice, particularly for interactions with the zona pellucida. Its absence leads to subfertility in male knockout mice, with fertilization rescue observed when the zona pellucida is removed during in vitro experiments.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biochemical Research Methods
Xueyan Liu, Yong Xu, Ran Wang, Sheng Liu, Jun Wang, YongLun Luo, Kwong-Sak Leung, Lixin Cheng
Summary: MoonFinder v2.0 is an algorithm dedicated to predicting human mlncRNAs, providing a comprehensive framework and a moonlighting coefficient for assessing their cellular functions. Studies have shown that mlncRNAs exhibit specific expression characteristics in sepsis and tend to be mutually exclusive in terms of coexpression compared to other lncRNAs.
BRIEFINGS IN BIOINFORMATICS
(2021)
Article
Agriculture, Dairy & Animal Science
Jose Luis Gutierrez Hernandez, Erika Gabriela Palomares Resendiz, Efren Diaz Aparicio, Victor Manuel Diaz Sanchez, Jorge Pablo Acosta Dibarrat, Victor Ruben Tenorio Gutierrez, Jorge Luis Tortora Perez
Summary: The study found that naturally infected rams with Brucella ovis had a higher concentration of antisperm antibodies in their blood sera, which could be associated with the immune response against the bacteria.
SMALL RUMINANT RESEARCH
(2022)
Article
Marine & Freshwater Biology
Alexandre M. Schonemann, Ricardo Beiras, Angel P. Diz
Summary: This study assessed quantitative proteomic changes in the liver of adult male Sheepshead minnow exposed to ethinyl estradiol (EE2). The findings provide valuable insights into the impact of estrogenic endocrine disruption in marine fish and identify potential molecular biomarkers for estrogen exposure in the environment.
AQUATIC TOXICOLOGY
(2022)
Article
Multidisciplinary Sciences
Coline Mahe, Regis Lavigne, Emmanuelle Com, Charles Pineau, Yann Locatelli, Aleksandra Maria Zlotkowska, Carmen Alminana, Guillaume Tsikis, Pascal Mermillod, Jennifer Schoen, Marie Saint-Dizier
Summary: Understanding the composition of oviduct fluid is crucial for understanding the microenvironment of sperm capacitation, fertilization, and early embryo development. This study analyzed the proteome of oviduct fluid samples from different regions, stages, and sides of the oviduct, and found that protein abundance was mainly influenced by the oviduct region, followed by the proximity of the ovulating ovary and the peri-ovulatory stage.
SCIENTIFIC REPORTS
(2022)
Article
Obstetrics & Gynecology
Andrea R. Thurman, Thomas R. Moench, Marshall Hoke, Joseph A. Politch, Howard Cabral, Emilie Mausser, Ellena Nador, Josh Morton, Krystal Hamorsky, Kelsi Swope, Barry Bratcher, Deborah J. Anderson, Kevin J. Whaley
Summary: ZB-06, a vaginal film containing a human contraceptive antibody, was found to be safe and effective in reducing the number of motile sperm in ovulatory cervical mucus when used before intercourse. Further development and testing of ZB-06 as a viable contraceptive candidate is warranted.
AMERICAN JOURNAL OF OBSTETRICS AND GYNECOLOGY
(2023)
Article
Multidisciplinary Sciences
M. A. M. Sayed, Hanan H. Abd-Elhafeez, O. S. Afifi, M. W. Marzouk, Taymour M. El-Sherry
Summary: A unique sperm behavior of forming motile thread-like bundles through agglutination was observed in Egyptian chickens. The study found that Sharkasi roosters exhibited a higher tendency to form longer and thicker bundles compared to Dandarawi roosters. The research also revealed that Sharkasi roosters had stronger sperm competition ability and produced a higher percentage of hatchlings.
SCIENTIFIC REPORTS
(2022)
Article
Biochemistry & Molecular Biology
Himani Tandon, Alexandre G. de Brevern, Narayanaswamy Srinivasan
Summary: Analysis of 58 protein-protein complexes revealed that approximately 55% of cases showed significant dynamics change upon binding, even without accompanying structural change. Binding of a protein partner was found to alter inter-residue communication within the tertiary structure in about 90% of cases, and accessible residue motions in unbound proteins were not always maintained in the bound form. Overall, the results strongly suggest that alteration of protein dynamics due to binding is a common occurrence.
Review
Cell Biology
Lucie Tumova, Michal Zigo, Peter Sutovsky, Marketa Sedmikova, Pavla Postlerova
Summary: The interaction between sperm and the zona pellucida (ZP) is crucial for successful fertilization in mammals, involving the binding of sperm surface ligands to complementary carbohydrates of ZP. Understanding the mechanisms of sperm-ZP interaction is critical for diagnosing infertility and improving assisted reproductive therapies/technologies.
Article
Multidisciplinary Sciences
Inessa Penner, Mario Dejung, Anja Freiwald, Falk Butter, Jia-Xuan Chen, Bodo Plachter
Summary: Human cytomegalovirus (HCMV) is a highly medically relevant pathogen, and Subviral Dense Bodies (DB) have been developed as a vaccine candidate to mitigate the severe consequences of HCMV infection. To develop a candidate vaccine for human application, a comprehensive mass spectrometry (MS)-based analysis was performed to study the proteome changes in cell culture cells exposed to DB.
Article
Pathology
Bryce D. Warren, Soo H. Ahn, Kathryn S. Brittain, Manjunatha K. Nanjappa, Hao Wang, Jianrong Wang, Gustavo Blanco, Gladis Sanchez, Yong Fan, Brian K. Petroff, Paul S. Cooke, Margaret G. Petroff
Summary: The deficiency in autoimmune regulator (AIRE) can lead to male infertility due to impaired central immune tolerance, resulting in reduced mating frequency, hypogonadism, and increased autoantibodies against reproductive targets. This highlights the critical role of AIRE-dependent central tolerance in maintaining male fertility and preventing autoimmunity against reproductive organs.
AMERICAN JOURNAL OF PATHOLOGY
(2021)
Review
Biotechnology & Applied Microbiology
Leonor Resina, Carlos Aleman, Frederico Castelo Ferreira, Teresa Esteves
Summary: Antibodies are considered the gold standard for recognition affinity tools due to their high selectivity and sensitivity. However, their production cost and downstream processing are expensive and time-consuming. Molecularly imprinted polymers (MIPs), specifically designed with molecular recognition sites, have emerged as cheaper and easier alternatives to antibodies. Research on MIPs for protein and peptide recognition has gained importance in various biomedical applications, including biosensors and targeted drug delivery systems. This mini review focuses on recent advances in (bio)molecular imprinting technology for sensing and therapeutic applications, discussing template selection, immobilization approaches, monomers, and highlighting potential drawbacks and research gaps.
BIOTECHNOLOGY ADVANCES
(2023)
Article
Biochemistry & Molecular Biology
Wei Qin, Joleen S. Cheah, Charles Xu, James Messing, Brian D. Freibaum, Steven Boeynaems, J. Paul Taylor, Namrata D. Udeshi, Steven A. Carr, Alice Y. Ting
Summary: The ability to map trafficking for thousands of endogenous proteins at once in living cells reveals biology currently invisible to microscopy and mass spectrometry. The method TransitID uses proximity labeling enzymes TurboID and APEX to map endogenous proteome trafficking with nanometer spatial resolution. TransitID allows for distinguishing protein populations based on their origin compartment or cell type.
Article
Obstetrics & Gynecology
Loren Mear, Charles Pineau, Francois Vialard, Juan-Felipe Velez de la Calle
Summary: This study aims to improve the detection rate of endometriosis by identifying high-risk patients and reducing diagnostic delay. By analyzing IVF patients, it was found that sperm agglutination was significantly correlated with endometriosis and CA-125 levels. A decision tree was built to identify a subpopulation of patients with low CA-125 levels and a high risk of endometriosis.
Article
Multidisciplinary Sciences
An Thys, Kilian Trillet, Sara Rosinska, Audrey Gayraud, Tiphaine Douanne, Yannic Danger, Clotilde C. N. Renaud, Luc Antigny, Regis Lavigne, Charles Pineau, Emmanuelle Com, Franck Verite, Julie Gavard, Nicolas Bidere
Summary: The adaptor SHARPIN, together with E3 ligases HOIP and HOIL1, forms the LUBAC complex, which catalyzes the addition of linear ubiquitin chains to substrates. This process is crucial for regulating NF-kappa B signaling downstream of immunoreceptors. Phosphorylation of SHARPIN at serine 165 plays a role in controlling linear ubiquitination of NEMO, affecting NF-kappa B activation in response to TNF alpha. These findings provide insights into the regulation of LUBAC and potential strategies for modulating its activity.
Article
Genetics & Heredity
Chloe Puisney-Dakhli, Francesca Gubana, Francois Petit, Hanane Bouchghoul, Valerie Gautier, Jelena Martinovic, Gerard Tachdjian, Aline Receveur
Summary: This case report describes a 14-week gestation fetus with a deletion in the FOXF1 gene detected through chorionic villous sampling, leading to alveolar capillary dysplasia with misalignment of pulmonary veins (ACDMPV) on fetopathological examination. The complex genomic data generated by ultrasound markers can be interpreted effectively through genotype-phenotype correlations during pathological examination of the fetus.
AMERICAN JOURNAL OF MEDICAL GENETICS PART A
(2021)
Article
Genetics & Heredity
Claire-Marine Berat, Celina Roda, Anais Brassier, Juliette Bouchereau, Camille Wicker, Aude Servais, Sandrine Dubois, Murielle Assoun, Claire Belloche, Valerie Barbier, Virginie Leboeuf, Francois M. Petit, Pauline Gaignard, Elise Lebigot, Pierre-Jean Berat, Clement Pontoizeau, Guy Touati, Cecile Talbotec, Florence Campeotto, Chris Ottolenghi, Jean-Baptiste Arnoux, Pascale de Lonlay Pascale
Summary: This study retrospectively analyzed the use of ETF in patients with inherited metabolic diseases at Necker Hospital in Paris, France, and found that ETF is an essential component of dietary management for IMDs. The study also highlighted potential value of gastrostomy as complications were more common with nasogastric tube feeding and the duration of ETF varied among different disease subgroups.
MOLECULAR GENETICS AND METABOLISM REPORTS
(2021)
Article
Biotechnology & Applied Microbiology
Ozlem Yilmaz, Amelie Patinote, Emmanuelle Com, Charles Pineau, Julien Bobe
Summary: This study utilized liquid chromatography and tandem mass spectrometry to analyze the proteomic profiles of zebrafish eggs lacking different types of Vtgs, revealing unique contributions of Vtgs to protein and energy homeostasis, and significant impacts on embryonic development. The findings suggest that the global egg proteome can be extensively modified depending on the presence or absence of specific Vtgs, affecting developmental competence.
Article
Biochemical Research Methods
Mira Merdas, Melanie Lagarrigue, Quentin Vanbellingen, Thierry Umbdenstock, Georges Da Violante, Charles Pineau
Summary: Matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI MSI) is a crucial tool for analyzing biological tissues, providing spatial and quantitative information about different analytes. However, low detection sensitivity of some compounds remains a challenge, which can be addressed by on-tissue chemical derivatization (OTCD) methods, commonly used for detecting small exogenous or endogenous molecules. OTCD reagents can also simplify sample preparation by serving as a matrix in addition to reacting with targeted chemical functions.
JOURNAL OF MASS SPECTROMETRY
(2021)
Article
Biochemistry & Molecular Biology
Melanie Simoes Eugenio, Florence Faurez, Ghania H. Kara-Ali, Melanie Lagarrigue, Perrine Uhart, Marion C. Bonnet, Isabelle Gallais, Emmanuelle Com, Charles Pineau, Michel Samson, Jacques Le Seyec, Marie-Therese Dimanche-Boitrel
Summary: TRIM21 has been identified as a new partner of the TRAIL-induced necrosome, acting as a positive regulator of the necroptosis process.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2021)
Article
Chemistry, Analytical
Mira Merdas, Melanie Lagarrigue, Thierry Umbdenstock, Antoine Lhumeau, Francoise Dartiguelongue, Quentin Vanbellingen, Georges Da Violante, Charles Pineau
Summary: The study utilized matrix-assisted laser desorption/ionization mass spectrometry imaging to investigate the distribution of acetaminophen and its metabolites in tissues. By employing on-tissue chemical derivatization, the sensitivity of mass spectrometry imaging for detecting the drug and its metabolites was enhanced, allowing for quantitative analysis in complex anatomical structures.
ANALYTICAL CHEMISTRY
(2021)
Article
Oncology
Maud Charpentier, Emilie Dupre, Agnes Fortun, Floriane Briand, Mike Maillasson, Emmanuelle Com, Charles Pineau, Nathalie Labarriere, Catherine Rabu, Francois Lang
Summary: The main challenge in cancer immunotherapy is selecting relevant tumor antigens. The study investigates the translation control of MELOE-1 and its role in melanoma cells. It shows that promoting hnRNP-A1 translation in tumor cells enhances MELOE-1 recognition and the efficacy of immunotherapies.
MOLECULAR ONCOLOGY
(2022)
Article
Medicine, General & Internal
Loren Mear, Emmanuelle Com, Khadija Fathallah, Laetitia Guillot, Regis Lavigne, Blandine Guevel, Arnaud Fauconnier, Francois Vialard, Charles Pineau
Summary: Endometriosis is a common chronic gynaecological disease that can lead to infertility and chronic pain. By comparing proteins in the eutopic endometrium of women with and without endometriosis, potential biomarker candidates were identified, offering new insights into the mechanisms of the disease. Further confirmation on a larger sample cohort is needed to validate these findings.
Article
Multidisciplinary Sciences
Dusan Zivkovic, Angelique Sanchez Dafun, Thomas Menneteau, Adrien Schahl, Sandrine Lise, Christine Kervarrec, Ana Toste Rego, Paula C. A. da Fonseca, Matthieu Chavent, Charles Pineau, Odile Burlet-Schiltz, Julien Marcoux, Marie-Pierre Bousquet
Summary: During spermatogenesis, the proteasome plays a crucial role in regulating cell division processes. The s20S proteasome becomes highly active during meiosis, mainly through 19S activation and PA200 binding. The proteasome population shifts from c20S to s20S during differentiation, and s20S interacts with components of the meiotic synaptonemal complex. In vitro, s20S shows higher trypsin- and chymotrypsin-like activities and displays structural differences between alpha 4 and alpha 4s.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Multidisciplinary Sciences
Coline Mahe, Regis Lavigne, Emmanuelle Com, Charles Pineau, Yann Locatelli, Aleksandra Maria Zlotkowska, Carmen Alminana, Guillaume Tsikis, Pascal Mermillod, Jennifer Schoen, Marie Saint-Dizier
Summary: Understanding the composition of oviduct fluid is crucial for understanding the microenvironment of sperm capacitation, fertilization, and early embryo development. This study analyzed the proteome of oviduct fluid samples from different regions, stages, and sides of the oviduct, and found that protein abundance was mainly influenced by the oviduct region, followed by the proximity of the ovulating ovary and the peri-ovulatory stage.
SCIENTIFIC REPORTS
(2022)
Article
Cell Biology
Charles Banliat, Coline Mahe, Regis Lavigne, Emmanuelle Com, Charles Pineau, Valerie Labas, Benoit Guyonnet, Pascal Mermillod, Marie Saint-Dizier
Summary: A comprehensive study of proteome dynamics in non-rodent mammalian embryos developed in vivo was conducted. Proteomic changes in bovine embryos at different developmental stages were analyzed, and distinct patterns were observed. Proteins that increased in abundance during development were associated with RNA metabolism, protein translation, and ribosome biogenesis, while proteins that decreased in abundance were involved in Golgi vesicle transport and protein processing in endoplasmic reticulum. The results provide valuable insights into the molecular mechanisms underlying early embryo development.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Food Science & Technology
Julie Le Faouder, Bastien Arnaud, Regis Lavigne, Celine Lucas, Emmanuelle Com, Elodie Bouvret, Anne-Laure Dinel, Charles Pineau
Summary: The supplementation of fish hydrolysates has shown to prevent stress-induced dysregulation of proteins, indicating its potential as an innovative strategy for stress management.
Article
Genetics & Heredity
Floriane Lejamtel, Cecile Oheix, Elisa Morales, Jelena Martinovic, Philippe Labrune, Francois Mickael Petit, Aline Receveur, Nelly Achour-Frydman, Alexandra Benachi, Chloe Puisney-Dakhli, Alexandre Joseph Vivanti
Summary: Increasing identification of neuro-susceptibility copy number variations (CNVs) has led to difficulties in predicting phenotypic features. The survey shows that multidisciplinary consultation is the key point in managing PIEV CNVs before conducting family surveys, which should be the basis for French recommendations.