Review
Biology
Minerva Codruta Badescu, Lacramioara Ionela Butnariu, Alexandru Dan Costache, Liliana Gheorghe, Petronela Nicoleta Seritean Isac, Adriana Chetran, Sabina Andreea Leanca, Irina Afrasanie, Stefania-Teodora Duca, Eusebiu Vlad Gorduza, Irina Iuliana Costache, Ciprian Rezus
Summary: Factor V Leiden and prothrombin G20210A are the most common hereditary thrombophilias and their relationship with acute myocardial infarction is still uncertain. Screening should be implemented in select cases and followed by optimal control of traditional cardiovascular risk factors for prevention. Extended dual antiplatelet therapy may be considered due to the lower bleeding risk conferred by FV Leiden.
Review
Medicine, General & Internal
Jaskamal Padda, Khizer Khalid, Ayushi Mohan, Sindhu Pokhriyal, Nitya Batra, Gazala Hitawala, Ayden Charlene Cooper, Gutteridge Jean-Charles
Summary: Factor V Leiden (FVL) G1619A mutation and prothrombin gene (PTG) G20210A are the most common inherited thrombophilias, with prevalence in Caucasians but controversial regarding routine screening. The association and conclusions vary among different ethnic groups and studies, necessitating further high-quality research for conclusive results.
CUREUS JOURNAL OF MEDICAL SCIENCE
(2021)
Article
Endocrinology & Metabolism
Lamia M'barek, Salma Sakka, Fatma Meghdiche, Dhaker Turki, Khadija Maalla, Mariem Dammak, Choumous Kallel, Chokri Mhiri
Summary: The study confirmed the involvement of MTHFR polymorphisms as important risk factors for AIS, while the presence of FVL polymorphism or prothrombin G20210A mutation alone is not correlated with stroke occurrence. Patients with both MTHFR and FVL polymorphisms have a synergistic effect, increasing the risk of AIS.
METABOLIC BRAIN DISEASE
(2021)
Article
Hematology
Beate Luxembourg, Franziska Henke, Anette Kirsch-Altena, Ulrich Sachs, Bettina Kemkes-Matthes
Summary: Double heterozygotes for Factor V Leiden and Prothrombin G20210A exhibit a higher risk of venous thromboembolism in female patients compared to other genotype groups, developing VTE at a younger age. However, there is no significant difference in spontaneous VTE and arterial thromboembolic events compared to other genotypes. Deep vein thrombosis in the lower limb is the predominant VTE location in double heterozygotes.
THROMBOSIS RESEARCH
(2021)
Article
Virology
Aslihan Kiraz, Ozlem Sezer, Adem Alemdar, Sezin Canbek, Nilgun Duman, Atil Bisgin, Tulin Cora, Hatice Ilgin Ruhi, Mahmut Cerkez Ergoren, Bilgen Bilge Geckinli, Sebnem Ozemri Sag, Hilmi Erdem Gozden, Ozlem Oz, Zuhal Mert Altintas, Sinem Yalcintepe, Adem Keskin, Ayseguel Yabaci Tak, Seyma Aktas Paskal, Ugur Fahri Yurekli, Mercan Demirtas, Emine Unal Evren, Abdullah Hanta, Mueserref Basdemirci, Kaya Suer, Burhan Balta, Nadir Kocak, Halil Guerhan Karabulut, Havva Cobanogullari, Esra Arslan Ates, Sevcan Tug Bozdogan, Damla Eker, Sadiye Ekinci, Sueleyman Nergiz, Timur Tuncali, Serap Yagbasan, Ceren Alavanda, Nuket Yurur Kutlay, Hakan Evren, Murat Erdogan, Sule Altiner, Tamer Sanlidag, Gizem Akinci Gonen, Arzu Vicdan, Nazan Eras, Hatice Kocak Eker, Ozgur Balasar, Gulten Tuncel, Munis Dundar, Hakan Gurkan, Sehime Gulsun Temel
Summary: This study examined the impact of hereditary thrombophilia factors Prothrombin (FII) and Factor V Leiden (FVL) genotypes on the severity of COVID-19 disease and the development of thrombosis. FVL gene variation was found to be associated with disease severity and thrombus formation in COVID-19 patients, while Prothrombin mutations had no impact on disease severity. Therefore, possible FVL gene mutations should be investigated in COVID-19 patients and appropriate treatment should be initiated early in FVL-positive patients.
JOURNAL OF MEDICAL VIROLOGY
(2023)
Article
Health Care Sciences & Services
Petra Riedlova, Dagmar Kramna, Silvie Ostrizkova, Hana Tomaskova, Vitezslav Jirik
Summary: Screening for factor V Leiden and factor II prothrombin in young women using ddPCR may effectively prevent TED and be cost-effective in the Czech Republic. The cost-benefit analysis suggests that screening for these genetic risk factors before starting OCs could significantly reduce treatment costs associated with TED.
Article
Medicine, General & Internal
Marcia Machado, Marta Cunha, Filipe Goncalves, Carlos Fernandes, Jorge Cotter
Summary: Venous thromboembolism (VTE) is a chronic illness with many associated risk factors. Anticoagulation therapy is crucial in managing VTE, and the duration of treatment depends on the patient's risk.
CUREUS JOURNAL OF MEDICAL SCIENCE
(2023)
Review
Obstetrics & Gynecology
Andrea Busnelli, Federico Cirillo, Paolo Emanuele Levi-Setti
Summary: The study investigated the impact of controlled ovarian hyperstimulation (COH) for in vitro fertilization (IVF) on thyroid function and found a significant increase in serum TSH levels during IVF, especially in women with hypothyroidism. New thyroid function screening strategies for women undergoing COH are warranted.
FERTILITY AND STERILITY
(2021)
Article
Medicine, General & Internal
Junyu Zhai, Shang Li, Yinci Zhu, Yun Sun, Zi-Jiang Chen, Yanzhi Du
Summary: Serum SHBG concentration is positively correlated with ovarian response in non-PCOS patients, but not in PCOS patients.
FRONTIERS IN MEDICINE
(2021)
Article
Hematology
M. G. Nikolaeva, A. P. Momot, M. S. Zainulina, N. N. Yasafova, I. A. Taranenko
Summary: This study aimed to investigate the association between Factor II activity and the G20210A mutation with the development of great obstetrical syndromes. Higher Factor II activity was found to be linked with early preeclampsia and fetal growth retardation. Threshold values for Factor II activity with the G20210A mutation were identified for predicting the development of preeclampsia.
THROMBOSIS JOURNAL
(2021)
Article
Endocrinology & Metabolism
Runan Hu, Yanjing Huang, Yufan Song, Xiao Wu, Kunkun Song, Guangying Huang, Mingmin Zhang, Haoxu Dong
Summary: This study aimed to investigate the effect of acupuncture on improving the pregnancy rate of COH rats by regulating the opening time of the implantation window and endometrial receptivity. Acupuncture was performed on COH rats at specific acupoints for 7 times. The results showed that acupuncture significantly increased the pregnancy rate, restored abnormal hormone levels, and reversed the advancement of implantation window in COH rats. Additionally, acupuncture restored the expression levels of ER, PR, LIF, integrin beta 3, VEGF, and FGF-2 in the endometrium to varying degrees. Therefore, acupuncture may enhance endometrial receptivity and improve the pregnancy rate of COH rats.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Stefano Palomba, Flavia Costanzi, Scott M. Nelson, Aris Besharat, Donatella Caserta, Peter Humaidan
Summary: The aim of this study was to evaluate and grade interventions for the prevention and reduction of OHSS in IVF patients. The results showed that follitropin delta may reduce the incidence of early OHSS in low-risk IVF patients, while in high-risk patients, inositol pretreatment, low-dose urinary hCG for ovulation triggering, and luteal phase administration of a GnRH antagonist may reduce the risk of OHSS.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Endocrinology & Metabolism
Ning Huang, Lixue Chen, Ying Lian, Hongbin Chi, Jie Qiao
Summary: This study aimed to investigate the impact of TSH levels after controlled ovarian hyperstimulation (COH) on IVF/ICSI outcomes. The results showed that mildly elevated TSH levels did not affect IVF/ICSI outcomes, but strict control of TSH levels may increase the risk of preterm delivery.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Article
Obstetrics & Gynecology
Jessica R. Zolton, Saioa Torrealday
Summary: In vitro fertilization is suitable for PCOS patients, especially those who have not responded to ovulation induction agents or are considered high-risk for multiple gestations. Choosing the right treatment protocol and medication dosage, as well as close monitoring, can reduce the risk of ovarian hyperstimulation syndrome during treatment.
CLINICAL OBSTETRICS AND GYNECOLOGY
(2021)
Article
Endocrinology & Metabolism
Yizhi Yan, Ruomu Qu, Xiaodong Ma, Siyuan Qin, Lixue Chen, Xiaoxiao Ni, Rui Yang, Ying Wang, Rong Li, Jie Qiao
Summary: Based on dynamic changes of indicators during controlled ovarian hyperstimulation and clinical outcomes of suboptimal ovarian response with different protocols, this study summarized the clinical characteristics of SOR and provided relevant clinical recommendations, demonstrating that SOR has adverse effects on clinical outcomes.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Respiratory System
Francesco Salton, Paola Confalonieri, Stefano Centanni, Michele Mondoni, Nicola Petrosillo, Paolo Bonfanti, Giuseppe Lapadula, Donato Lacedonia, Antonio Voza, Nicoletta Carpene, Marcella Montico, Nicolo Reccardini, Gianfranco Umberto Meduri, Barbara Ruaro, Marco Confalonieri
Summary: This study compared the efficacy of high-dose methylprednisolone and conventional dexamethasone in the treatment of COVID-19 pneumonia and found that methylprednisolone did not reduce mortality at 28 days.
EUROPEAN RESPIRATORY JOURNAL
(2023)
Article
Environmental Sciences
Laura Brunelli, Sofia Bussolaro, Raffaella Dobrina, Chiara De Vita, Elena Mazzolini, Giuseppa Verardi, Maura Degrassi, Maria Piazza, Andrea Cassone, Anja Starec, Giuseppe Ricci, Sara Zanchiello, Tamara Stampalija
Summary: To improve maternal and child health, a study was conducted to investigate the needs and expectations of expectant and new parents as potential users of a hypothetical application for the first 1000 days of life. The study found that women generally agreed with the proposed content, but expressed low interest in certain app functionalities or features. The findings provide insights for designers and technology providers to develop tailored mHealth solutions for the first 1000 days of life.
INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH
(2023)
Review
Health Care Sciences & Services
Alessandro Mangogna, Giada Munari, Francesco Pepe, Edoardo Maffii, Pierluigi Giampaolino, Giuseppe Ricci, Matteo Fassan, Umberto Malapelle, Stefania Biffi
Summary: Homologous recombination deficiency (HRD) refers to the inability to efficiently repair DNA double-strand breaks using the homologous recombination repair pathway. It serves as a predictive biomarker for the clinical use of poly (adenosine diphosphate [ADP]-ribose) polymerase inhibitors and platinum-based chemotherapy in ovarian cancers. However, HRD is a complex genomic signature, and various methods of analysis have been developed for HRD testing in the clinical setting.
JOURNAL OF PERSONALIZED MEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Chiara Agostinis, Miriam Toffoli, Andrea Balduit, Alessandro Mangogna, Hadida Yasmin, Chiara Ragazzon, Silvia Pegoraro, Giuseppina Campisciano, Guglielmo Stabile, Gabriella Zito, Uday Kishore, Manola Comar, Federica Scrimin, Roberta Bulla, Giuseppe Ricci
Summary: Although only a small percentage of SARS-CoV-2 infections occur in children aged 0-9, pneumonia remains the leading cause of infant mortality worldwide. Vaccinated breastfeeding mothers produce antibodies against the spike protein of SARS-CoV-2, which can activate the complement pathway and potentially protect breastfed newborns against infection. A study involving vaccinated healthcare and school workers found that anti-S IgG antibodies were present in the serum and breast milk, capable of activating complement.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Giuseppina Campisciano, Nunzia Zanotta, Mariachiara Quadrifoglio, Annalisa Careri, Alessandra Torresani, Carolina Cason, Francesco De Seta, Giuseppe Ricci, Manola Comar, Tamara Stampalija
Summary: In this study, the presence of bacterial DNA in the chorionic villi and amniotic fluids of pregnant women was investigated. The findings suggest a potential connection between the uterine microbiome and oral and vaginal dysbiosis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Alessandra Gianoncelli, Gabriela Sena Souza, George Kourousias, Ernesto Pascotto, Paul Tafforeau, Elena Longo, Regina Cely Barroso, Murielle Salome, Marco Stebel, Federica Zingaro, Carla Calligaro, Giuseppe Ricci, Lorella Pascolo
Summary: A better understanding of ovary structures is needed for advancement in folliculogenesis and reproductive medicine, specifically for fertility preservation in prepubertal girls with cancer. Current analysis methods involve histological sections, staining, and 2D microscopic inspection, but synchrotron radiation microtomography is a new and promising technique for 3D studies. This study compared four staining protocols using iodine or tungsten-based agents on bovine ovarian tissues fixed in Bouin's solution, and found that different agents highlighted different features of the structures. X-ray Fluorescence mapping confirmed that the tungsten-based agent had better penetration in this type of tissue.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Luisa Zupin, Sotiris Psilodimitrakopoulos, Fulvio Celsi, Lina Papadimitriou, Anthi Ranella, Sergio Crovella, Giuseppe Ricci, Emmanuel Stratakis, Lorella Pascolo
Summary: In this study, a unique morphological feature of 50B11 nociceptive sensory neurons in in vitro culture was revealed, which was related to forskolin-induced differentiation of these cells growing upside-down on cover glass supports. Multi-photon non-linear microscopy was used to observe increased neurite arborization and elongation. Second harmonic generation microscopy allowed for monitoring microtubule organization and cellular multi-photon autofluorescence. This work is the first to examine neuronal cell lines growing and differentiating in an upside-down orientation, providing potential improvements for the study of sensory neurons in peripheral nervous system disease and analgesic drug screening.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Lucia Merolle, Lorella Pascolo, Luisa Zupin, Pietro Parisse, Valentina Bonanni, Gianluca Gariani, Sasa Kenig, Diana E. Bedolla, Sergio Crovella, Giuseppe Ricci, Stefano Iotti, Emil Malucelli, George Kourousias, Alessandra Gianoncelli
Summary: Although X-ray fluorescence microscopy is widely used for single-cell analysis, sample preparation remains a major challenge. The information on sample treatment is inadequate and unclear, leading to wasted time and jeopardizing the success of the experiment. Various fixation methods have been described, but none have been systematically tested for synchrotron X-ray microscopy.
Article
Virology
Giulia Brisotto, Marcella Montico, Matteo Turetta, Stefania Zanussi, Maria Rita Cozzi, Roberto Vettori, Romina Boschian Boschin, Lorenzo Vinante, Fabio Matrone, Alberto Revelant, Elisa Palazzari, Roberto Innocente, Giuseppe Fanetti, Lorenzo Gerratana, Mattia Garutti, Camilla Lisanti, Silvia Bolzonello, Milena Sabrina Nicoloso, Agostino Steffan, Elena Muraro
Summary: Cellular and humoral immunity are both crucial in SARS-CoV-2 infection recovery and vaccine efficacy. The impact of mRNA vaccination on immune responses in healthy and fragile individuals is still being investigated. This study examined the cellular and humoral immune responses after vaccination in healthy subjects and cancer patients to assess antibody levels, cellular immunity, and the influence of cancer on vaccination efficacy. The findings highlight the correlation between higher antibody titers and positive cellular immunity, as well as the stability of T-cell responses over time compared to antibody levels, particularly in cancer patients.
Review
Biotechnology & Applied Microbiology
Barbara Bortot, Alessandro Mangogna, Giovanni Di Lorenzo, Guglielmo Stabile, Giuseppe Ricci, Stefania Biffi
Summary: Surgical resection is the main treatment for solid tumors, but accurate and safe intraoperative assessment of tumor margins is clinically necessary. Positive surgical margins have negative effects on treatment outcomes and survival. Image-guided surgery using nanoparticles as contrast agents shows promise in reducing positive surgical margins and improving resection accuracy. However, more research is needed to fully utilize nanotechnology in surgical imaging.
JOURNAL OF NANOBIOTECHNOLOGY
(2023)
Review
Medicine, General & Internal
Moira Barbieri, Giulia Zamagni, Ilaria Fantasia, Lorenzo Monasta, Leila Lo Bello, Mariachiara Quadrifoglio, Giuseppe Ricci, Gianpaolo Maso, Monica Piccoli, Daniela Denis Di Martino, Enrico Mario Ferrazzi, Tamara Stampalija
Summary: The objectives of this study were to review the available umbilical vein blood flow volume (UV-Q) reference ranges in uncomplicated pregnancies and compare them with UV-Q values obtained from a local cohort. Literature in English was reviewed according to PRISMA guidelines. Original articles were grouped based on UV sampling sites and formulae used to compute UV-Q. The UV-Q values were derived from formulae or tables presented by authors. A prospective observational study of uncomplicated singleton pregnancies was conducted to compare UV-Q with the systematic review results. Overall, there was a substantial heterogeneity in reported UV-Q central values, but values overlapped when using the same sampling methodology and formulae. The study suggests that UV-Q assessment is accurate and reproducible when adhering to the same methodology, encouraging further investigation on clinical applications.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Pharmacology & Pharmacy
Giovanni Canil, Marco Orleni, Bianca Posocco, Sara Gagno, Alessia Bignucolo, Marcella Montico, Rossana Roncato, Serena Corsetti, Michele Bartoletti, Giuseppe Toffoli
Summary: Poly (ADP-ribose) polymerase inhibitors (PARPis) are increasingly important in oncology, and their therapeutic drug monitoring (TDM) could benefit patients. This study developed and validated a liquid chromatography-tandem mass spectrometry method for quantifying olaparib, rucaparib, and niraparib in both human plasma and dried blood spot (DBS). The correlation between drug concentrations measured in these two matrices was assessed. Results showed a strong correlation between plasma and DBS for olaparib and niraparib, though further data is needed to establish a robust regression analysis for rucaparib. The study provides a solid foundation for the feasibility of using both plasma and DBS matrices for PARPis TDM.
Review
Oncology
Guglielmo Stabile, Chiara Ripepi, Lara Sancin, Stefano Restaino, Francesco Paolo Mangino, Luigi Nappi, Giuseppe Ricci
Summary: There are currently no guidelines for the optimal treatment of primary cervical lymphomas. Conservative treatment with surgery and chemotherapy or surgery and radiotherapy has shown apparent success in a few cases. Primary lymphomas of the female genital tract are rare, and they can affect various parts such as the uterus, cervix, vulva, vagina, or adnexa. The most common clinical presentation is prolonged minor abnormal uterine bleeding, while unstoppable bleeding is rarely reported.
Article
Engineering, Electrical & Electronic
Linjie Yan, Sudan Han, Chengpeng Hao, Danilo Orlando, Giuseppe Ricci
Summary: In this paper, a solution is proposed to the open problem of joint adaptive detection of multiple point-like targets in scenarios characterized by different clutter types. Detection architectures are devised to classify range bins and detect possible multiple targets with unknown positions and numbers. The proposed architectures enhance the entire detection and estimation performance of the system by making it aware of the surrounding environment.
IEEE TRANSACTIONS ON SIGNAL PROCESSING
(2023)
Article
Biochemistry & Molecular Biology
Valeria Capaci, Lorenzo Monasta, Michelangelo Aloisio, Eduardo Sommella, Emanuela Salviati, Pietro Campiglia, Manuela Giovanna Basilicata, Feras Kharrat, Danilo Licastro, Giovanni Di Lorenzo, Federico Romano, Giuseppe Ricci, Blendi Ura
Summary: This study utilized a multi-omics approach to investigate common dysregulated pathways in type 1 and type 2 endometrial cancer (EC) using four EC cell lines. The analysis identified shared pathways and identified specific proteins and metabolites involved in EC tumor growth. These findings provide insights into the mechanisms underlying EC development and growth.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)