4.7 Article

CFTR is essential for sperm fertilizing capacity and is correlated with sperm quality in humans

Journal

HUMAN REPRODUCTION
Volume 25, Issue 2, Pages 317-327

Publisher

OXFORD UNIV PRESS
DOI: 10.1093/humrep/dep406

Keywords

CFTR; human sperm; fertilizing capacity; sperm quality

Funding

  1. National 973 project [2006CB504002]
  2. Nature Science fund of China [30600217, 30670788]
  3. Nature Science fund of Zhejiang [Y2090236]

Ask authors/readers for more resources

Our previous studies have demonstrated the cystic fibrosis transmembrane conductance regulator (CFTR) is important for capacitation and male fertility in mouse and guinea pig spermatozoa. However, the exact function of CFTR on human sperm fertilizing capacity, and correlation with sperm quality has not been established. The present study may shed light on some unexplained male infertility, and on a possible new method for diagnosis of male infertility and strategy for male contraception. To assess the effect of CFTR on human sperm fertilizing capacity, we examined sperm capacitation and the acrosome reaction using chlortetracycline staining, analyzed sperm hyperactivation by computer-assisted semen analysis (CASA), measured intracellular cAMP levels using ElA and evaluated sperm penetration of zona-free hamster eggs assay in fertile men. The percentage of spermatozoa expressing CFTR from fertile, healthy and infertile men (mainly teratospermic, asthenoteratospermic, asthenospermic and oligospermic) was conducted by indirect immunofluorescence staining. Progesterone significantly facilitated human sperm capacitation and ZP3 triggered the acrosome reaction, both were significantly inhibited by CFTR inhibitor-172 (CFTRinh-172; 10 nM-1 mu M) in a dose-dependent manner. The presence of 100 nM CFTRinh-172 markedly depressed intracellular cAMP levels, sperm hyperactivation and sperm penetration of zona-free hamster eggs. In addition, the percentage of spermatozoa expressing CFTR in the fertile men was significantly higher than healthy and infertile men categories (P < 0.01). CFTR is essential for human sperm fertilizing capacity and the impairment of CFTR expression in spermatozoa is correlated with a reduction of sperm quality. These results suggest that defective expression of CFTR in human sperm may lead to the reduction of sperm fertilizing capacity.

Authors

I am an author on this paper
Click your name to claim this paper and add it to your profile.

Reviews

Primary Rating

4.7
Not enough ratings

Secondary Ratings

Novelty
-
Significance
-
Scientific rigor
-
Rate this paper

Recommended

Article Obstetrics & Gynecology

Comparison of recombinant human FSH biosimilar QL1012 with Gonal- f ® for ovarian stimulation: a phase-three trial

Linli Hu, Jieqiang Lv, Qianhong Ma, Xiujuan Chen, Xiaohong Wang, Xiaoyan Liang, Dingfei Xu, Yanping Li, Yuanhua Huang, Lina Hu, Xiaohui Deng, Jing Wei, Feng Zhou, Songying Zhang, Jie Wang, Fang Ma, Yingpu Sun

Summary: The study aimed to compare the equivalence of QL1012 and Gonal-f((R)) in ovarian stimulation for assisted reproductive technology. The results showed that there were no significant differences between QL1012 and Gonal-f((R)) in the primary endpoint of oocytes retrieved, and they had similar safety profiles.

REPRODUCTIVE BIOMEDICINE ONLINE (2023)

Article Physics, Multidisciplinary

Theoretical study of electron-impact broadening for highly charged Ar XV ion lines

Chao Wu, Xiang Gao, Yu-Hao Zhu, Xiao-Ying Han, Bin Duan, Ju Meng, Song-Bin Zhang, Jun Yan, Yong Wu, Jian-Guo Wang

Summary: In this study, the relativistic R-matrix method is used to investigate the electron-impact broadening of spectral lines produced by highly charged Ar XV ions. The results considering resonance structures differ significantly from the distorted wave approach. A new empirical formula with a correction term is proposed to account for the effect of resonances in a relatively wide range of plasma conditions. The fitting parameters for all 47 calculated transitions are provided, with an estimated accuracy within 1%.

CHINESE PHYSICS B (2023)

Article Materials Science, Ceramics

Mechanical properties of B4C-CrB2 binary eutectic composites prepared by arc-melting

Rong Tu, Jiaojiao Li, Mingpu Jia, Qizhong Li, Meijun Yang, Song Zhang, Takashi Goto

Summary: B4C-CrB2 composites were prepared by arc-melting using B4C and CrB2 powders as raw materials. The eutectic composition of B4C-CrB2 system was 30B(4)C-70CrB(2) (mol%) with a labyrinth-like irregularly layered eutectic microstructure. The composites exhibited maximum Vickers hardness (24.6 GPa) and fracture toughness (4.3 MPa m(1/2)) at room temperature.

INTERNATIONAL JOURNAL OF APPLIED CERAMIC TECHNOLOGY (2023)

Review Endocrinology & Metabolism

Regulators involved in trophoblast syncytialization in the placenta of intrauterine growth restriction

Hanjing Zhou, Chenqiong Zhao, Peixin Wang, Weijie Yang, Haiyan Zhu, Songying Zhang

Summary: Placental dysfunction refers to impaired placental function and inadequate supply of oxygen and nutrients to the fetus, resulting in fetal growth restriction. Deficient trophoblast syncytialization, inadequate trophoblast invasion, and impaired vascular remodeling contribute to placental dysfunction. Abnormal expression of proteins and signaling pathways involved in trophoblast syncytialization has been found in the placentas of pregnancies complicated by intrauterine growth restriction (IUGR).

FRONTIERS IN ENDOCRINOLOGY (2023)

Article Materials Science, Multidisciplinary

Influence of load frequency and corrosive environments on fatigue behavior of as-extruded Mg-Zn-Zr-Nd alloy

Wenchao Hu, Wanyi Huang, Ying Sun, Wen Zhang, Lili Tan, Song Zhang, Guangcai Ma, Dan Zhang, Qiang Wang

Summary: In this study, fatigue properties of Mg-2Zn-0.5Zr-0.5Nd alloy were tested and it was found that the fatigue life varies with different frequencies. It was also observed that the alloy exhibits poorer fatigue resistance in 0.9 wt% NaCl solution.

JOURNAL OF MATERIALS RESEARCH AND TECHNOLOGY-JMR&T (2023)

Article Surgery

Are testicular sperms superior to ejaculated sperms in couples with previous ART failure due to high rate of fragmented embryos? A retrospective cohort study

Ling-Ying Jiang, Fei-Fei Kong, Lv Yao, Fu-Xing Zhang, Sha-Sha Wang, Xiao-Ying Jin, Xiao-Mei Tong, Song-Ying Zhang

Summary: The study aimed to investigate whether using testicular sperm reduces embryo fragmentation and improves cycle outcomes. The results showed that patients who underwent intracytoplasmic sperm injection (ICSI) with testicular sperm had significantly lower fragmented embryo rate, higher transferable embryo rate, and higher implantation rate compared to those who used ejaculated sperm.

FRONTIERS IN SURGERY (2023)

Article Chemistry, Multidisciplinary

Synthesis of a crystalline zeolitic imidazole framework-8 nano-coating on single environment-sensitive viral particles for enhanced immune responses

Liuyang Wang, Xuan Lin, Yanan Sheng, Hongyu Zhu, Zhengjun Li, Zhiguo Su, Rong Yu, Songping Zhang

Summary: The encapsulation of antigens with ZIF-8 provides advantages in vaccine development, but the synthesis conditions of ZIF-8 can be too harsh for sensitive viral antigens. This study explores the synthesis of ZIF-8 on an environment-sensitive antigen and demonstrates the importance of size and morphology in exerting adjuvant effects.

NANOSCALE ADVANCES (2023)

Article Nanoscience & Nanotechnology

Optimizing Electromagnetic Interference Shielding of Ultrathin Nanoheterostructure Textiles through Interfacial Engineering

Song Zhang, Chongjie Wang, Tenghua Gao, Jinrong Hu, Pengjian Lu, Bingjian Guo, Qingfang Xu, Kai Liu, Baowen Li, Rong Tu, Meijun Yang, Kazuya Ando

Summary: Interfacial engineering can be used to obtain EMI shielding composites with thin thickness. In this study, a Ni nanoparticle/SiC nanowhisker/carbon cloth nanoheterostructure was fabricated, and the formation of a Ni/SiC interface was found to enhance dielectric loss and suppress reflection, resulting in excellent EMI shielding effectiveness. The material also exhibits high flexibility and long-term stability.

ACS APPLIED MATERIALS & INTERFACES (2023)

Article Chemistry, Applied

Base-Controlled Chemoselective Reaction of High-Valent Cu(III)-CF3 Compounds with Anilines: Access to Formamides or Hydrazines

Zhen-Mei Zheng, Ning Chen, Ming-Suo Dai, Song-Lin Zhang

Summary: This study reports the reactivity properties of Cu(III)-CF3 compounds with anilines, showing distinct reactivities based on their basicity, resulting in the formation of formamides or hydrazines. This is achieved through the competing generation of difluorocarbene and N-centered radicals, controlled by the basicity of the reaction solution. The study demonstrates the unusual use of high-valent Cu(III) trifluoromethyl compounds as hydrogen-abstracting reagents for dehydrogenative reactions.

ADVANCED SYNTHESIS & CATALYSIS (2023)

Article Chemistry, Applied

Geminal/Vicinal Amino- and Oxy-Trifluoromethylation of ortho-Ethynyl Anilines and Phenols by Cu(III)-CF3 Compounds

Dou-Dou Chen, Song-Lin Zhang

Summary: This study reports the geminal amino- and oxy-trifluoromethylation of ortho-ethynyl anilines/ phenols using phenCu(III)(CF3)(3), which provides a concise and controllable synthesis of valuable trifluoromethylated N and O-heterocyclic compounds. The method circumvents the regioselectivity issue often encountered in C H trifluoromethylation of arenes/heteroarenes and suppresses the competing vicinal fluoro-trifluoromethylation of terminal alkynes. The synthetic value of Cu(III) CF3 compounds in medicinal chemistry is highlighted.

ADVANCED SYNTHESIS & CATALYSIS (2023)

Article Chemistry, Physical

Understanding of deposition mechanism of vanadium on LiF with large mismatch by facing target sputtering (FTS)

Xiaole Song, Long Zheng, Rong Tu, Baifeng Ji, Jun Li, Song Zhang

Summary: V/LiF is an important window material for investigating phase transition of materials under ultra-high pressure, which is a fundamental issue in condensed matter physics. However, the large mismatch in lattice and thermal properties makes vanadium growth on LiF a big challenge. In this study, over 1.5 μm thick vanadium films were successfully grown on LiF using facing target sputtering (FTS), and transmission electron microscopy (TEM) observation revealed vertically well-aligned columnar crystals with numerous micro-sized gaps in the grown vanadium films. Particle-in-cell Monte Carlo collision (PIC-MCC) and Kinetic Monte Carlo (KMC) methods were used to simulate plasma discharge behavior, sputtered atom transport, and film growth to understand the deposition mechanism. A growth model of the vanadium film was established based on experimental and simulation results, and the micro-sized gaps in the columnar crystals were found to contribute to the release of residual stresses caused by the large mismatch, which can be explained by the grain boundary relaxation model.

APPLIED SURFACE SCIENCE (2023)

Article Multidisciplinary Sciences

Contribution of labor related gene subtype classification on heterogeneity of polycystic ovary syndrome

Jue Zhou, Zhou Jiang, Leyi Fu, Fan Qu, Minchen Dai, Ningning Xie, Songying Zhang, Fangfang Wang

Summary: The study aimed to identify molecular subtypes and related genes associated with preterm labor risk in women with polycystic ovary syndrome (PCOS). Through analysis of multiple gene expression datasets, two molecular subtypes were identified in PCOS patients, and key genes related to preterm labor were determined. These findings help uncover the heterogeneity of clinical pregnancy complications in women with PCOS.

PLOS ONE (2023)

Article Engineering, Manufacturing

On the correlation between processing parameters, plastic deformation, and mechanical property in hard machining of H13 steel

Binxun Li, Song Zhang, Yujing Sun, Jin Du

Summary: In this paper, a systematic investigation was conducted on the correlation of processing parameters, plastic deformation, and mechanical properties of the machined surface layer for hardened H13 steel. The results indicate that edge micro-geometry significantly influences the depth of the plastic deformation layer, and the automated ball indentation (ABI) technique was used to assess the mechanical properties of the machined surface layers. The study reveals the relationship between processing parameters, depth of plastic deformation, and mechanical properties.

PROCEEDINGS OF THE INSTITUTION OF MECHANICAL ENGINEERS PART B-JOURNAL OF ENGINEERING MANUFACTURE (2023)

Article Chemistry, Multidisciplinary

Laser CVD Growth of Uniquely <010>-oriented β-Ga2O3 Films on Quartz Substrate with Ultrafast Photoelectric Response

Rong Tu, Xinyu Li, Qingfang Xu, Bao-Wen Li, Tenghua Gao, Xian Zhang, Song Zhang, Takashi Goto, Lianmeng Zhang

Summary: This study reports the first growth of uniquely <010>-oriented ss-Ga2O3 films on quartz substrates by laser chemical vapor deposition (LCVD). The <010> orientation is closely related to the higher stability of oxygen close-packed planes under O-rich condition. The <010>-oriented ss-Ga2O3 film-based MSM type detector exhibits ultra-fast response speed.

SMALL (2023)

Review Cardiac & Cardiovascular Systems

Prognostic value of growth differentiation factor-15 in patients with coronary artery disease: A meta-analysis and systematic review

Song Zhang, Panpan Hao, Jiaxin Li, Qi Zhang, Xiaoying Yin, Jiali Wang, Yuguo Chen

Summary: The study aims to investigate the effects of growth differentiation factor-15 (GDF-15) on all-cause death, cardiovascular death, MI, and stroke in patients with coronary artery disease (CAD). The results showed that patients with elevated GDF-15 levels had significantly increased risks of all-cause death, cardiovascular death, and MI, but a lower predictive effect on stroke. Further studies are needed to explore the association between GDF-15 and stroke outcome.

FRONTIERS IN CARDIOVASCULAR MEDICINE (2023)

No Data Available