Article
Genetics & Heredity
Van Khanh Tran, Ngoc-Lan Nguyen, Lan Ngoc Thi Tran, Phuong Thi Le, Anh Hai Tran, Tuan L. A. Pham, Nguyen Thi Kim Lien, Nguyen Thi Xuan, Le Tat Thanh, Thanh Van Ta, Thinh Huy Tran, Huy-Hoang Nguyen
Summary: This study identified seven pathogenic/likely pathogenic variants in the LAMA2 gene in six patients with congenital muscular dystrophy from five unrelated Vietnamese families, providing genetic etiology and counseling for their parents.
FRONTIERS IN GENETICS
(2023)
Article
Biochemistry & Molecular Biology
Jorge Alonso-Perez, Ana Carrasco-Rozas, Maria Borrell-Pages, Esther Fernandez-Simon, Patricia Pinol-Jurado, Lina Badimon, Lutz Wollin, Cinta Lleixa, Eduard Gallardo, Montse Olive, Jordi Diaz-Manera, Xavier Suarez-Calvet
Summary: This study found that nintedanib has a positive effect on a murine model of alpha-sarcoglycanopathy. Nintedanib can improve muscle function and architecture by reducing muscle fibrosis and degeneration and reverting the chronic inflammatory environment.
Article
Genetics & Heredity
P. A. Chausova, O. P. Ryzhkova, G. E. Rudenskaya, V. B. Chernykh, O. A. Shchagina, A. V. Polyakov
Summary: Merosine deficient congenital muscular dystrophy is a common form of muscular dystrophy caused by a genetic deficiency. New variants with this type of inheritance may be hidden in the genetic makeup of parents.
FRONTIERS IN GENETICS
(2021)
Article
Cardiac & Cardiovascular Systems
Emily D. D. Morales, Yongping Yue, Thais B. B. Watkins, Jin Han, Xiufang Pan, Aaron M. M. Gibson, Bryan Hu, Omar Brito-Estrada, Gang Yao, Catherine A. A. Makarewich, Gopal J. J. Babu, Dongsheng Duan
Summary: This study identified DWORF as a potential therapeutic target for Duchenne muscular dystrophy (DMD) cardiomyopathy. Gene therapy using adeno-associated virus serotype 9-DWORF vector significantly enhanced SERCA activity, reduced myocardial fibrosis, and improved exercise capacity, cardiac function, and hemodynamics in mdx mice. These findings suggest that DWORF gene therapy holds promise for treating DMD cardiomyopathy.
JOURNAL OF THE AMERICAN HEART ASSOCIATION
(2023)
Article
Cardiac & Cardiovascular Systems
Vivian Haffner, Zahra Nourian, Erika M. Boerman, Michelle D. Lambert, Laurin M. Hanft, Maike Krenz, Christopher P. Baines, Dongsheng Duan, Kerry S. McDonald, Timothy L. Domeier
Summary: In this study, the effects of Duchenne muscular dystrophy (DMD) on cardiomyocyte Ca2+ handling were investigated using a mouse model. The results indicate that DMD leads to Ca2+ overload in cardiomyocytes following preload challenge, as well as impaired cardiac function. These findings support the hypothesis that cardiomyocyte Ca2+ overload underlies cardiac dysfunction in muscular dystrophy.
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY
(2023)
Article
Cell Biology
Yuki Ashida, Koichi Himori, Nao Tokuda, Azuma Naito, Nao Yamauchi, Nana Takenaka-Ninagawa, Yoshitsugu Aoki, Hidetoshi Sakurai, Takashi Yamada
Summary: This study reveals the mechanisms underlying the disruption of excitation-contraction (EC) coupling in dystrophin-deficient muscles. The Ca2+-dependent proteolysis of SH3 and cysteine-rich domain 3 (STAC3) and junctophilin 1 (JP1) mediated by calpain-1 is identified in dystrophin-deficient muscle. The dissociation of STAC3 and JP1 from the dihydropyridine receptor is also highlighted as a causative factor in EC uncoupling in these muscles.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2022)
Article
Medicine, Research & Experimental
Andrea Farini, Luana Tripodi, Chiara Villa, Francesco Strati, Amanda Facoetti, Guido Baselli, Jacopo Troisi, Annamaria Landolfi, Caterina Lonati, Davide Molinaro, Michelle Wintzinger, Stefano Gatti, Barbara Cassani, Flavio Caprioli, Federica Facciotti, Mattia Quattrocelli, Yvan Torrente
Summary: Duchenne muscular dystrophy (DMD) is a progressive muscle wasting disease caused by mutations in the DMD gene. In this study, researchers investigated the role of intestinal bacteria in supporting the muscle immune response in a dystrophic mouse model. They found a correlation between DMD disease features and the abundance of Prevotella bacteria. Manipulating the gut microbial community through germ-free animal models and antibiotic treatment influenced muscle immunity and fibrosis. Intestinal colonization with beneficial bacteria improved inflammation, muscle pathology, and function. This study highlights a potential role for the gut microbiota in DMD pathogenesis.
EMBO MOLECULAR MEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Angus Lindsay, John Holm, Maria Razzoli, Alessandro Bartolomucci, James M. Ervasti, Dawn A. Lowe
Summary: Research shows that mdx mice do not habituate to mild stress, and daily exposure to mild stress for weeks exacerbates phenotypes associated with dystrophinopathy in mdx mice.
Article
Nanoscience & Nanotechnology
Chunjuan Zhang, Jongsoo Mok, Yeonwoo Seong, Hui-chong Lau, Dayeon Kim, Junsik Yoon, Seung Wook Oh, Tae Sub Park, Joonghoon Park
Summary: Cell-derived vesicles (CDVs) obtained from genetically engineered cells, specifically PROKR1(Tg) CDVs, show promise as an alternative therapy for muscular dystrophy by delivering PROKR1 protein and restoring myogenic potential in affected cells.
NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Caorui Lin, Gang Han, Lulu Jia, Yiwen Zhao, Jun Song, Ning Ran, Toshifumi Yokota, Yiqi Seow, HaiFang Yin
Summary: This study demonstrates that the combination of oral glycine and metformin with intravenous PMO can enhance the efficacy of PMO treatment for DMD. This combination therapy shows potential benefits for DMD patients and potentially other muscle diseases.
Article
Physiology
Angus Lindsay, Bailey Kemp, Alexie A. Larson, Cory W. Baumann, Preston M. McCourt, John Holm, Peter Karachunski, Dawn A. Lowe, James M. Ervasti
Summary: The study found that levels of tetrahydrobiopterin were low in Duchenne muscular dystrophy patients and mdx mice, but could be restored to normal levels by overexpressing dystrophin. Tetrahydrobiopterin deficiency in mdx mice was likely due to lower levels of sepiapterin reductase in skeletal muscle. Supplementation with tetrahydrobiopterin improved skeletal muscle function and cardiac pathology in mdx mice.
Article
Multidisciplinary Sciences
M. Osman Sheikh, Chantelle J. Capicciotti, Lin Liu, Jeremy Praissman, Dahai Ding, Daniel G. Mead, Melinda A. Brindley, Tobias Willer, Kevin P. Campbell, Kelley W. Moremen, Lance Wells, Geert-Jan Boons
Summary: Matriglycan plays a critical role in protein binding and viral infection, and its length can be adjusted accordingly. This finding contributes to a better understanding of the interaction between cells and viruses.
NATURE COMMUNICATIONS
(2022)
Review
Biochemistry & Molecular Biology
Christine Peladeau, Jagdeep K. Sandhu
Summary: Inflammasomes are crucial in maintaining tissue homeostasis, with NLRP3 inflammasome being activated by various stimuli, causing chronic inflammation and exacerbating neuromuscular diseases. Therapeutic targeting of NLRP3 components may offer potential benefits in alleviating detrimental effects of these diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell Biology
Angelika Svetlove, Jonas Albers, Swen Huelsmann, Marietta Andrea Markus, Jana Zschuentzsch, Frauke Alves, Christian Dullin
Summary: This study aimed to establish a sensitive and reliable method for assessing respiratory muscle weakness and irregular breathing patterns. Optical respiratory dynamics tracking (ORDT) was developed and successfully distinguished mice with Duchenne muscular dystrophy (DMD) from healthy controls by measuring expiration constants. ORDT was able to distinguish fast and slow expiratory phases, which could not be observed using X-ray based lung function measurements. The simplicity and reliability of ORDT were further validated using smartphone acquisition.
Article
Genetics & Heredity
Hamidreza Mianesaz, Safoura Ghalamkari, Mansoor Salehi, Mahdiyeh Behnam, Majid Hosseinzadeh, Keivan Basiri, Majid Ghasemi, Maryam Sedghi, Behnaz Ansari
Summary: In this study, next-generation sequencing was used to diagnose limb-girdle muscular dystrophy in 26 Iranian patients. Six novel disease-causing variants were identified, some of which affected highly conserved regions of amino acid sequence.
MOLECULAR GENETICS & GENOMIC MEDICINE
(2023)
Article
Oncology
Takako I. Jones, Oliver D. King, Charis L. Himeda, Sachiko Homma, Jennifer C. J. Chen, Mary Lou Beermann, Chi Yan, Charles P. Emerson, Jeffrey B. Miller, Kathryn R. Wagner, Peter L. Jones
CLINICAL EPIGENETICS
(2015)
Article
Cell Biology
Sachiko Homma, Mary Lou Beermann, Bryant Yu, Frederick M. Boyce, Jeffrey Boone Miller
Article
Biology
Hiroaki Mitsuhashi, Satoshi Ishimaru, Sachiko Homma, Bryant Yu, Yuki Honma, Mary Lou Beermann, Jeffrey Boone Miller
Article
Biochemistry & Molecular Biology
Sachiko Homma, Jennifer C. J. Chen, Fedik Rahimov, Mary Lou Beermann, Kendal Hanger, Genila M. Bibat, Kathryn R. Wagner, Louis M. Kunkel, Charles P. Emerson, Jeffrey Boone Miller
EUROPEAN JOURNAL OF HUMAN GENETICS
(2012)
Article
Biochemistry & Molecular Biology
Takako Iida Jones, Jennifer C. J. Chen, Fedik Rahimov, Sachiko Homma, Patricia Arashiro, Mary Lou Beermann, Oliver D. King, Jeffrey B. Miller, Louis M. Kunkel, Charles P. Emerson, Kathryn R. Wagner, Peter L. Jones
HUMAN MOLECULAR GENETICS
(2012)
Article
Biochemistry & Molecular Biology
Charis L. Himeda, Celine Debarnot, Sachiko Homma, Mary Lou Beermann, Jeffrey B. Miller, Peter L. Jones, Takako I. Jones
MOLECULAR AND CELLULAR BIOLOGY
(2014)
Article
Cell Biology
Soonsang Yoon, Guido Stadler, Mary Lou Beermann, Eric V. Schmidt, James A. Windelborn, Peter Schneiderat, Woodring E. Wright, Jeffrey Boone Miller
Article
Biology
Isabel F. Masteika, Anvitha Sathya, Sachiko Homma, Bess M. Miller, Frederick M. Boyce, Jeffrey Boone Miller
Summary: This study reveals the downstream events initiated by aberrant DUX4 expression and provides new possibilities for mechanistic investigation. The findings demonstrate that DUX4 expression alters nucleocytoplasmic protein transport, generates double-strand DNA breaks, and requires the Bax/Bak pathway for caspase activation.
Article
Biology
Mary Lou Beermann, Sachiko Homma, Jeffrey Boone Miller
Summary: This study developed a proximity ligation assay (PLA) for detecting DUX4 protein in facioscapulohumeral muscular dystrophy (FSHD) muscle biopsies. The assay was able to detect DUX4 protein in a small subset of myofibers, suggesting its potential usefulness for studying FSHD.
BMC RESEARCH NOTES
(2022)
Article
Clinical Neurology
Sachiko Homma, Mary Lou Beermann, Frederick M. Boyce, Jeffrey Boone Miller
ANNALS OF CLINICAL AND TRANSLATIONAL NEUROLOGY
(2015)