Article
Biochemistry & Molecular Biology
Rohan Dandage, Caroline M. Berger, Isabelle Gagnon-Arsenault, Kyung-Mee Moon, Richard Greg Stacey, Leonard J. Foster, Christian R. Landry
Summary: The study investigated protein-protein interactions in hybrids between yeast species, finding that most interactions are similar to those of the parents but with some altered instances. It suggests that the occurrence of chimeric protein complexes is frequent, potentially due to incompatibilities or imbalances between parental proteomes.
MOLECULAR BIOLOGY AND EVOLUTION
(2021)
Article
Food Science & Technology
Lin Lin, Rubing Du, Yuqiao Wang, Qun Wu, Yan Xu
Summary: This study revealed the cross-feeding of amino acids between Saccharomyces cerevisiae and two lactobacilli species in Chinese liquor fermentation. A predictive model was established to regulate the growth of lactobacilli species.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2022)
Article
Multidisciplinary Sciences
Jakob Peder Pettersen, Eivind Almaas
Summary: Improvements have been made to a new model for predicting the temperature dependence of an organism's metabolic network, addressing the issue of multimodality and highlighting the model's inadequacy in predicting metabolic flux with current experimental data.
SCIENTIFIC REPORTS
(2023)
Article
Biotechnology & Applied Microbiology
Cristobal A. Onetto, Anthony R. Borneman, Simon A. Schmidt
Summary: Interactions between non-Saccharomyces yeast species and Saccharomyces cerevisiae can impact fermentation performance, with strain selection playing a key role in optimizing fermentation outcomes when using non-Saccharomyces species.
FERMENTATION-BASEL
(2021)
Article
Biology
Cynthia Tang, Jimin Han, Sonal Dalvi, Kannan Manian, Lauren Winschel, Stefanie Volland, Celia A. Soto, Chad A. Galloway, Whitney Spencer, Michael Roll, Caroline Milliner, Vera L. Bonilha, Tyler B. Johnson, Lisa Latchney, Jill M. Weimer, Erika F. Augustine, Jonathan W. Mink, Vamsi K. Gullapalli, Mina Chung, David S. Williams, Ruchira Singh
Summary: Mutations in CLN3 lead to photoreceptor cell loss in CLN3 disease, affecting the phagocytosis of photoreceptor outer segments by retinal pigment epithelium (RPE) cells. Patient-derived cells show that CLN3 is essential for RPE cell structure and phagocytosis, suggesting potential for gene therapy targeting RPE cells in treating CLN3 disease.
COMMUNICATIONS BIOLOGY
(2021)
Article
Biochemical Research Methods
Pavani Vamsi Krishna Nittala, Allison Hohreiter, Emilio Rosas Linhard, Ryan Dohn, Suryakant Mishra, Abhiteja Konda, Ralu Divan, Supratik Guha, Anindita Basu
Summary: The paper presents fabrication methodologies that integrate silicon components into soft microfluidic devices for cell lysis. The integration methodology involves a silicon chip with microstructure arrays embedded in a microfluidic device, which is actuated by piezoelectric force to physically break microbial cell walls. Different silicon microarray geometries, fabrication techniques, integration methods, and efficacy evaluation using synthetic microbeads and yeast species are presented. The proposed integration methodology can serve as an important process step for future hybrid silicon-polymeric devices in cellular processing applications.
Article
Biochemistry & Molecular Biology
Petra Vande Zande, Patricia J. Wittkopp
Summary: This study uses expression and fitness data from Saccharomyces cerevisiae gene deletion strains to show that cis-acting mutations tend to have lower pleiotropy and trans-acting mutations are more deleterious. These findings provide strong support for the role of pleiotropy in the preferential fixation of cis-regulatory alleles over evolutionary time.
MOLECULAR BIOLOGY AND EVOLUTION
(2022)
Article
Multidisciplinary Sciences
William M. Shaw, Lucie Studena, Kyler Roy, Piotr Hapeta, Nicholas S. McCarty, Alicia E. Graham, Tom Ellis, Rodrigo Ledesma-Amaro
Summary: CRISPR gene activation and inhibition is a powerful synthetic tool for influencing gene expression, with applications in foundational studies, cellular reprogramming, and metabolic engineering. The authors developed a method for inducible expression of multiple gRNAs, increasing the capacity for CRISPR gene activation and inhibition and enhancing flexibility in target gene manipulation. They also created a highly regulated and user-friendly CRISPR toolkit in yeast, enabling simultaneous activation and repression of 11 genes in central metabolism, resulting in a significant increase in succinic acid production.
NATURE COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Weronika Rzepnikowska, Joanna Kaminska, Andrzej Kochanski
Summary: In this study, a simple yeast model was developed to evaluate the significance of IGHMBP2 gene mutations in the development of SMARD1. The results showed that the model can distinguish between pathogenic and non-pathogenic mutations, providing important insights for clinical diagnosis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Pharmacology & Pharmacy
Binxin Cui, Lin Lin, Bangmao Wang, Wentian Liu, Chao Sun
Summary: This review focuses on the therapeutic potential and mechanisms of S. boulardii as a probiotic, and summarizes its potential efficacy in various liver diseases and future prospects.
PHARMACOLOGICAL RESEARCH
(2022)
Review
Food Science & Technology
Patrizia Romano, Giacomo Braschi, Gabriella Siesto, Francesca Patrignani, Rosalba Lanciotti
Summary: The aromatic complexity of wine is mainly influenced by the interaction between grapes and fermentation agents, with yeast playing a crucial role in the formation of aroma compounds. Yeast can contribute to the formation of compounds from primary metabolism, synthesis of specific metabolites, and modification of molecules present in the must.
Article
Multidisciplinary Sciences
Nouf N. Laqtom, Wentao Dong, Uche N. Medoh, Andrew L. Cangelosi, Vimisha Dharamdasani, Sze Ham Chan, Tenzin Kunchok, Caroline A. Lewis, Ivonne Heinze, Rachel Tang, Christian Grimm, An N. Dang Do, Forbes D. Porter, Alessandro Ori, David M. Sabatini, Monther Abu-Remaileh
Summary: Lysosomes play crucial roles in cellular function, and dysfunction is associated with various human diseases. The study reveals the importance of CLN3 protein in glycerophospholipid metabolism within lysosomes, as its loss leads to massive accumulation of GPDs.
Article
Biotechnology & Applied Microbiology
Shiqin Yu, Mingjia Li, Song Gao, Jingwen Zhou
Summary: This study proposes several strategies to enhance the production of dihydroquercetin (DHQ) from naringenin (NAR) and reduce the accumulation of metabolites, providing a reference for related research.
MICROBIAL CELL FACTORIES
(2022)
Review
Food Science & Technology
Ana Mencher, Pilar Morales, Jordi Tronchoni, Ramon Gonzalez
Summary: The development of non-Saccharomyces starter cultures in oenology has led to increasing interest in the interactions between microorganisms during the transformation of grape must into wine. Interspecific interactions between wine yeasts involve various levels of competition and metabolic exchange, with indications of specific recognition in some cases. This article discusses the communication mechanisms between wine yeasts during alcoholic fermentation.
Review
Biochemistry & Molecular Biology
Laura Vanderwaeren, Ruveyda Dok, Karin Voordeckers, Sandra Nuyts, Kevin J. Verstrepen
Summary: Yeast is an important enzyme source for bread and beer production and is an ideal model organism for studying eukaryotic cell biology and genetics. This review discusses the characteristics that make yeast such a widely used model organism, with a focus on the DNA damage response pathway as an example of its contribution to elucidating highly conserved biological processes. Differences in the DNA damage response of yeast and humans are also highlighted, along with the challenges of using yeast as a model system.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)