Journal
HUMAN IMMUNOLOGY
Volume 75, Issue 2, Pages 124-128Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.humimm.2013.11.012
Keywords
Natural killer cells; Cell surface molecules; Killer cell immunoglobulin-like receptors; Polymorphism; Human
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Funding
- Office of Naval Research [N00014-11-1-0590]
- National Cancer Institute Cancer Center [CA051008]
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Natural killer cell stimulatory receptor gene, KIR2DS5, is polymorphic. While KIR2DS5*002 is most frequently observed, other alleles have also been found. The proteins encoded by these alleles (KIR2DS5*002-*009) are expressed at varying levels on the surface of NKL and Jurkat transfectants. Gel electrophoresis of all allelic products showed two isoforms which differ in the extent of maturation of N-linked glycosylation. These isoforms differed in intensity and molecular weight among the allelic products. Site-directed mutagenesis was used to identify polymorphic variation at residues 123 and 157 as key in altering glycosylation and levels of surface expression. (C) 2013 American Society for Histocompatibility and Immunogenetics. Published by Elsevier Inc. All rights reserved.
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