Article
Plant Sciences
Udhayaraj Suriyakalaa, Rajamanickam Ramachandran, Jaffar Ali Doulathunnisa, Smilin Bell Aseervatham, Devaraj Sankarganesh, Soundararajan Kamalakkannan, Balamuthu Kadalmani, Jayaraman Angayarkanni, Mohammad Abdulkader Akbarsha, Shanmugam Achiraman
Summary: The study found that extracts from the leaves of Ficus religiosa have a therapeutic effect on polycystic ovary syndrome (PCOS) in rats, possibly due to the presence of potential volatile molecules. Treatment with fresh leaves can regulate hormone levels and increase specific enzyme levels.
JOURNAL OF ETHNOPHARMACOLOGY
(2021)
Article
Reproductive Biology
Andressa Gonsioroski, Daryl D. Meling, Liying Gao, Michael J. Plewa, Jodi A. Flaws
Summary: Exposure to Iodoacetic acid altered estrous cyclicity, ovarian gene expression, and estradiol levels in mice, leading to changes in apoptotic factors, cell cycle regulators, steroidogenic factors, estrogen receptors, and oxidative stress markers.
BIOLOGY OF REPRODUCTION
(2021)
Article
Reproductive Biology
Edyta Rytelewska, Marta Kiezun, Katarzyna Kisielewska, Marlena Gudelska, Kamil Dobrzyn, Barbara Kaminska, Tadeusz Kaminski, Nina Smolinska
Summary: The study shows that chemerin plays a modulatory role in the synthesis of steroid hormones in pigs, affecting ovarian functions due to the heterogeneity of factors and interactions between them.
Article
Engineering, Environmental
Jing-Cai Liu, Chun-Hua Xing, Yi Xu, Zhen-Nan Pan, Hao-Lin Zhang, Yu Zhang, Shao-Chen Sun
Summary: Exposure to DEHP during lactation reduces follicle numbers in suckling mice, alters gene transcription levels leading to decreased estradiol and increased oxidative stress. DNA damage marker and ovarian apoptosis are increased in DEHP-exposed suckling mice, with inhibition of granulosa cell proliferation. These changes result in abnormal spindle formation and chromosome misalignment during oocyte maturation.
JOURNAL OF HAZARDOUS MATERIALS
(2021)
Article
Food Science & Technology
Angelica D. Reyes-Perea, Hilda M. Guerrero-Netro, Europa Meza-Serrano, Anthony Estienne, Christopher A. Price
Summary: This study found that deoxynivalenol (DON), a major mycotoxin in animal feed, negatively affects the growth and reproduction of farm animals. The study determined that in bovine theca cells, the metabolite of DON, de-epoxy-DON (DOM-1), activates endoplasmic stress pathways. However, DON only partially activates these pathways in granulosa cells.
Review
Medicine, Research & Experimental
Hanne Vlieghe, Ellen C. R. Leonel, Parinaz Asiabi, Christiani A. Amorim
Summary: Theca cells play multiple roles during folliculogenesis but their recruitment process and function are not well understood. This review aims to discuss the recent advancements in the characterization and origins of theca cells, their applications in vitro culture for fertility preservation and studies, and their importance in understanding pathogenic pathways and hormonal replacement therapies. Isolation and in vitro culture techniques have improved the understanding of theca cells as a specific cell population. Unraveling the origins of theca cells in the adult ovary will assist in developing hormonal replacement therapies and treating diseases influenced by theca cells.
Article
Food Science & Technology
Maria Rosa Ramos Nieto, Marianela Lasagna, Gabriel Cao, Gloria Alvarez, Clarisa Santamaria, Maria Eugenia Rodriguez Girault, Nadia Bourguignon, Noelia Di Giorgio, Clara Ventura, Mariana Mardirosian, Horacio Rodriguez, Victoria Lux-Llantos, Claudia Cocca, Mariel Nunez
Summary: The study found that chronic exposure to low concentrations of CPF affects ovarian steroid synthesis in female rats, leading to changes in the estrous cycle of animals, as well as proliferative changes in the uterus.
FOOD AND CHEMICAL TOXICOLOGY
(2021)
Article
Multidisciplinary Sciences
Adam J. Ziecik, Pawel Likszo, Jan Klos, Katarzyna Gromadzka-Hliwa, Katarzyna Knapczyk-Stwora, Olli Peltoniemi, Zdzislaw Gajewski, Monika M. Kaczmarek
Summary: Ovarian cysts can reduce reproductive performance in pigs, and the formation mechanism of lutein cysts is still unknown. This study compared the endocrine and molecular milieus of healthy preovulatory follicles, healthy and atretic-like follicles induced by gonadotropins, and gonadotropin-provoked and spontaneous ovarian cysts in gilts, and found significant differences in endocrine and molecular indicators and microRNA in the walls of cysts. The results suggest a novel mechanism of lutein ovarian cysts development in pigs and possibly other species.
SCIENTIFIC REPORTS
(2023)
Article
Environmental Sciences
Dai Lin, Yiqin Chen, Lisheng Liang, Zuxiong Huang, Yiwei Guo, Ping Cai, Wenxiang Wang
Summary: The study found that exposure to TNT explosive can lead to changes in the menstrual cycle, decrease in preantral follicle proportion, increase in atretic follicle proportion, and changes in hormone levels, possibly due to disruptions in steroidogenesis.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2023)
Article
Agriculture, Dairy & Animal Science
Yejee Park, Yoon-Been Park, Seok-Won Lim, Byeonghwi Lim, Jun-Mo Kim
Summary: This research identified dynamic changes in gene expression in the pig ovary during the estrous cycle through transcriptome analysis. The study revealed that gene expression changes were more activated in the luteal phase, and the metestrus and diestrus periods were important for pregnancy preparation or the next estrous cycle after ovulation.
Article
Immunology
Jaya Bharati, Satish Kumar, N. H. Mohan, Bikash Chandra Das, Salam Jayachitra Devi, Vivek Kumar Gupta
Summary: This study provides insight into the role of differentially expressed genes and signaling pathways in follicular development and maturation in Ghoongroo pigs, highlighting the wide-ranging mechanisms involved in follicular transition and the intra-follicular control of ovarian function.
JOURNAL OF REPRODUCTIVE IMMUNOLOGY
(2023)
Article
Agriculture, Dairy & Animal Science
Rafael H. Alvarez, Keila M. R. Duarte, Joao B. P. Carvalho, Cecilia C. Rocha, A. A. Gilmar Junior, Eduardo Trevisol, Alfredo J. F. Melo, Guilherme Pugliesi
Summary: This study investigates the ovarian volume, antral follicle count (AFC), and ovarian dynamics in cows at different ages. The results show that as cows age, their ovarian volume increases and the number of antral follicles decreases. However, the growth pattern of follicles and the functionality of corpus luteum do not seem to be affected by age.
ANIMAL REPRODUCTION SCIENCE
(2023)
Article
Toxicology
Masahiro Ogawa, Junya Kitamoto, Takeo Takeda, Masami Hori, Kisako Shikano, Amami Yamanaka-Tanaka, Tomoki Tanaka, Tomoya Kawaguchi, Megumi Terada, Taku Tanaka
Summary: Sex hormones are primarily produced in the gonads and adrenal cortex. Endocrine-disrupting chemicals (EDCs) mimic, block, or interfere with hormones in the endocrine systems. Several assays are used to identify the disruption potential of nuclear receptors, with the H295R steroidogenesis assay being the only one detecting steroidogenesis disruption. To establish a short-term screening method for steroidogenesis disruption, rats were fed forskolin, prochloraz, or D5 for 14 days. The results emphasize the importance of comprehensively assessing the endocrine-disrupting effects of chemicals on steroidogenesis in vivo.
JOURNAL OF APPLIED TOXICOLOGY
(2023)
Article
Developmental Biology
Patrycja Kurowska, Monika Sroka, Monika Dawid, Ewa Mlyczynska, Natalia Respekta, Malgorzata Jurek, Dominika Klimczyk, Malgorzata Grzesiak, Joelle Dupont, Agnieszka Rak
Summary: The study found that resistin and its receptors exhibit different expression levels in the porcine corpus luteum during different luteal phases, and they can be regulated by hormones. Additionally, resistin can regulate progesterone and estradiol secretion by influencing steroidogenic enzyme expression and kinase phosphorylation.
Review
Biochemistry & Molecular Biology
Camilla H. K. Hughes, Bruce D. Murphy
Summary: The development of ovarian follicles to ovulation is essential for fertility, with nuclear receptors playing key roles in regulating granulosa cell proliferation, hormone synthesis, and final stages of follicular growth. LRH-1 is a crucial nuclear receptor in these processes, and other receptors like ESR2 and AR also play important roles.
MOLECULAR ASPECTS OF MEDICINE
(2021)
Article
Cell Biology
Monika Dawid, Ewa Mlyczynska, Malgorzata Jurek, Natalia Respekta, Karolina Pich, Patrycja Kurowska, Wiktoria Gieras, Tomasz Milewicz, Malgorzata Kotula-Balak, Agnieszka Rak
Summary: The apelinergic system, including the apelin receptor (APJ) and its specific ligands, plays an important role in regulating placental physiology and pregnancy. Imbalances in placental processes may lead to specific pregnancy pathologies, highlighting the need to find substances for early diagnosis or treatment.
Article
Biochemistry & Molecular Biology
Ewa Mlyczynska, Alice Bongrani, Christelle Rame, Malgorzata Wegiel, Anna Maslanka, Piotr Major, Piotr Zarzycki, Pierre-Henri Ducluzeau, Arnaud De Luca, Celine Bourbao-Tournois, Pascal Froment, Agnieszka Rak, Joelle Dupont
Summary: This study aimed to determine the concentrations of polycyclic aromatic hydrocarbons (PAHs), especially naphthalene, in the plasma and adipose tissue of French and Polish bariatric patients and investigate their correlation with body mass index (BMI), adipokines expression levels, and the effects of naphthalene on cell proliferation and differentiation. The results showed that PAHs and naphthalene could potentially be obesogenic molecules, as naphthalene concentration in adipose tissue was positively correlated with BMI and adipokines expression in French patients. Moreover, naphthalene exposure increased cell proliferation and differentiation, suggesting an involvement in adipogenesis/lipogenesis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Cell Biology
Natalia Respekta, Karolina Pich, Monika Dawid, Ewa Mlyczynska, Patrycja Kurowska, Agnieszka Rak
Summary: The apelinergic system, consisting of apelin, ELABELA, and the apelin receptor APJ, plays a multifaceted role in various biological functions. This review focuses on the role of the apelinergic system in cell apoptosis and highlights the signaling pathways involved, such as PI3K, ERK1/2, AKT, AMPK, and PKA. Dysregulation of apoptosis can lead to pathological conditions, and the apelinergic system shows potential as a therapeutic target in various diseases.
Article
Agriculture, Dairy & Animal Science
Ewa Mlyczynska, Ewa Zaobidna, Edyta Rytelewska, Kamil Dobrzy, Grzegorz Kopij, Karolina Szymanska, Patrycja Kurowska, Cecylia Dall'Aglio, Nina Smolinska, Tadeusz Kaminski, Agnieszka Rak
Summary: Visfatin/NAMPT is a hormone that connects energy metabolism with female reproduction. This study investigates the expression and localization of visfatin in the corpus luteum (CL) and its regulation by hormones such as LH, insulin, progesterone, prostaglandin E2, and F2 alpha. The study finds that visfatin expression in the porcine CL depends on the hormonal status of the estrous cycle or early pregnancy and is regulated by LH, insulin, progesterone, and prostaglandins through the ERK1/2 pathway.
ANIMAL REPRODUCTION SCIENCE
(2023)
Article
Reproductive Biology
Patrycja Kurowska, Monika Dawid, Julia Oprocha, Natalia Respekta, Loise Serra, Anthony Estienne, Piotr Pawlicki, Malgorzata Kotula-Balak, Fabrice Guerif, Joelle Dupont, Agnieszka Rak
Summary: This study investigates the expression and regulatory function of the novel neuropeptide Spexin (SPX) in the human ovary, and its potential role in polycystic ovarian syndrome (PCOS) pathogenesis. The results show that SPX levels are decreased in obese and PCOS women, and SPX inhibits granulosa cell (GC) proliferation and steroidogenesis. GALR2/3 and kinases MAP3/1, AKT, and STAT3 are involved in mediating these effects.
BIOLOGY OF REPRODUCTION
(2023)
Article
Reproductive Biology
Ewa Mlyczynska, Patrycja Kurowska, Dominika Wachowska, Malgorzata Grzesiak, Joelle Dupont, Agnieszka Rak
Summary: This study investigated the expression of phoenixin-14 in porcine corpus luteum and its impact on endocrine function. The results showed that phoenixin-14 can regulate the secretion of progesterone and estradiol and affect the expression of hormone receptors in luteal cells. Moreover, G protein-coupled receptor 173 and extracellular signal-regulated kinase 1/2 play important roles in the regulation of endocrine function in luteal cells.
BIOLOGY OF REPRODUCTION
(2023)
Article
Reproductive Biology
Ewa Mlyczynska, Patrycja Kurowska, Edyta Rytelewska, Ewa Zaobina, Karolina Pich, Marta Kiezun, Kamil Dobrzyn, Katarzyna Kisielewska, Grzegorz Kopij, Nina Smolinska, Tadeusz Kaminski, Agnieszka Rak
Summary: Recent studies have shown that visfatin is involved in the regulation of female reproduction. This study aimed to investigate the expression and localization of visfatin in the ovarian follicles of prepubertal and mature gilts, and the effects of various hormones on visfatin levels. The results showed that visfatin expression is dependent on the maturity of the animals and the stage of ovarian follicle development. The study also revealed that visfatin is regulated by different hormones and may play a crucial role in porcine ovarian follicular function.
Article
Multidisciplinary Sciences
Karolina Szymanska, Ewa Zaobidna, Edyta Rytelewska, Ewa Mlyczynska, Patrycja Kurowska, Kamil Dobrzyn, Marta Kiezun, Barbara Kaminska, Nina Smolinska, Agnieszka Rak, Tadeusz Kaminski
Summary: Visfatin is expressed in both the anterior and posterior lobes of the pig pituitary, and its expression is influenced by the estrous cycle and early pregnancy. GnRH, FSH, LH, and insulin can stimulate the secretion of Visfatin in pituitary cells, with LH specifically enhancing Visfatin release during days 2 to 3 of the cycle.
SCIENTIFIC REPORTS
(2023)
Article
Cell Biology
Karolina Pich, Jesika Rajewska, Kinga Kaminska, Marcelina Tchurzyk, Agata Szlaga, Patryk Sambak, Anna Blasiak, Malgorzata Grzesiak, Agnieszka Rak
Summary: This study aimed to investigate the impact of VD3 supplementation on RARRES2 and ADIPOQ levels in the uterus of PCOS rats. The findings showed that VD3 supplementation restored the levels of these adipokines and increased the expression of their receptors in the uterus of PCOS rats, indicating a new mechanism of VD3 action in uterine physiology.
Article
Biochemistry & Molecular Biology
Malgorzata Opydo, Anna Mlyczynska, Ewa Mlyczynska, Agnieszka Rak, Elzbieta Kolaczkowska
Summary: Acute myeloid leukemia (AML) is a blood cancer characterized by abnormal cell proliferation and inhibition of apoptosis. The MCL-1 protein plays a critical role in the survival of AML cells. This study investigated the effects of a specific MCL-1 inhibitor, S63845, alone and in combination with other inhibitors, on AML cell lines. The results showed that S63845 decreased cell viability and induced apoptosis, and combined treatment enhanced apoptosis and induced cell differentiation. These findings suggest the potential use of MCL-1 inhibitors in combination with other pro-survival protein inhibitors.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Reproductive Biology
Patrycja Kurowska, Ewa Mlyczynska, Julia Wajda, Konrad Krol, Karolina Pich, Patrycja Guzman, Aleksandra Greggio, Oliwia Szkraba, Malgorzata Opydo, Joelle Dupont, Agnieszka Rak
Summary: This study investigated the expression of PNX-14 and GPR173 in ovarian tissue and the effects of PNX-14 on granulosa cell function. The results showed that PNX-14 can influence ovarian follicle growth by promoting granulosa cell proliferation, hormone secretion, and cell cycle progression.
REPRODUCTIVE BIOLOGY
(2024)
Article
Multidisciplinary Sciences
Natalia Respekta, Karolina Pich, Ewa Mlyczynska, Kamil Dobrzyn, Christelle Rame, Tadeusz Kaminski, Nina Smolinska, Joelle Dupont, Agnieszka Rak
Summary: This study investigated the expression and regulation of OMNT1 in the porcine anterior pituitary during the estrous cycle. The results showed fluctuation in the expression of OMNT1 in different stages of the cycle and differences in expression levels between pig breeds. GnRH and gonadotropins had different effects on OMNT1 protein expression.
SCIENTIFIC REPORTS
(2023)
Article
Physiology
P. Kurowska, K. Gazdzik, A. Jasinska, E. Mlyczynska, D. Wachowska, A. Rak
Summary: The formation and regression of the corpus luteum are controlled by various factors. Imbalance between proliferation and apoptosis processes can lead to deficient luteal phase and infertility. Resistin has been found to enhance viability and stimulate autophagy in porcine luteal cells, promoting the maintenance of luteal cell function.
JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY
(2023)
Article
Veterinary Sciences
Malgorzata Kotula-Balak, Piotr Pawlicki, Anna Galuszka, Laura Pardyak, Ryszard Tuz, Klaudia Dubniewicz, Anna Skrzypczak-Wiercioch, Agnieszka Rak, Monika Dawid, Kazimierz Tarasiuk
Summary: This study revealed, for the first time, the effects of GnRH vaccination on the testicular function of Landrace pigs. It showed disrupted adiponectin and leptin signaling, increased cholesterol concentration, and attenuated spermatogenesis due to halted androstenone production. The altered GnRH signaling may impact the adipokine system in the testes, leading to further functional changes and lipid metabolism disturbances.
MEDYCYNA WETERYNARYJNA-VETERINARY MEDICINE-SCIENCE AND PRACTICE
(2022)
Review
Endocrinology & Metabolism
Patrycja Kurowska, Ewa Mlyczynska, Monika Dawid, Natalia Respekta, Karolina Pich, Loise Serra, Joelle Dupont, Agnieszka Rak
Summary: The prevalence of adult obesity has increased significantly, and the endocrine system plays a crucial role in energy balance and fat deposition. Endocrine disruptor chemicals (EDCs) have been extensively studied for their association with obesity. EDCs can trigger obesity through various mechanisms, such as influencing adipocyte differentiation, increasing triglyceride storage, or elevating adipocyte number. Furthermore, EDCs and obesity may affect reproductive functions and lead to conditions like polycystic ovarian syndrome and decreased sperm motility.