Article
Cell & Tissue Engineering
Judith Pape, Deniz Bakkalci, Rawiya Al Hosni, Benjamin S. Simpson, Kristiina Heikinheimo, Stefano Fedele, Umber Cheema
Summary: This study aimed to create a fully humanized 3D disease model containing ameloblastoma cells, osteoblasts, and activated osteoclasts in order to investigate the RANKL pathway within the ameloblastoma stromal environment and its response to the RANKL antibody denosumab.
JOURNAL OF TISSUE ENGINEERING
(2022)
Article
Immunology
Chanhyeok Jeon, Yurin Jang, Subin Weon, Hyosun Park, Suein Lee, Younseo Oh, Sung Hoon Choi, Sung Eun Wang, Tae-Hwan Kim, Il-Hoon Sung, Sungsin Jo
Summary: This study found that ankylosing spondylitis (AS) patients have elevated kynurenine (Kyn) levels, which are closely related to disease activity. During osteoblast differentiation, treatment with Kyn promotes bone mineralization and inhibits osteoclast formation. These findings are important for understanding the pathological bone features and disease mechanisms of AS.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2023)
Article
Medical Laboratory Technology
Liuqing Wang, Li Hua, Xuelian Hong, Fang Chen, Hongwei Du
Summary: This study investigated the association between serum anti-carbamylated protein (anti-CarP) antibodies and disease activity and bone loss in rheumatoid arthritis (RA). The results showed that RA patients had significantly higher levels of serum anti-CarP antibodies compared to non-RA patients and healthy controls, and these levels were positively correlated with disease activity. Furthermore, the concentrations of anti-CarP antibodies were significantly increased in anti-CCP-positive RA patients. There was also a positive correlation between anti-CarP antibodies and RANKL, which is related to bone loss. These findings suggest that anti-CarP antibodies are associated with RA disease activity and may play a role in bone loss associated with RA. The concentration of anti-CarP antibodies could potentially be used as a novel biomarker for early diagnosis of RA.
CLINICA CHIMICA ACTA
(2023)
Editorial Material
Biotechnology & Applied Microbiology
Xiao-Man Sun, Ying-Shuang Xu, He Huang
Summary: Thraustochytrids can accumulate high biomass and lipid content, exhibit broad substrate utilization capacity and effective metabolic pathways without genetic modification, showing great promise for future development.
TRENDS IN BIOTECHNOLOGY
(2021)
Article
Multidisciplinary Sciences
Dane Huang, Chao Zhao, Ruyue Li, Bingyi Chen, Yuting Zhang, Zhejun Sun, Junkang Wei, Huihao Zhou, Qiong Gu, Jun Xu
Summary: This study reports an approach to identify selective and druggable binding sites on protein surfaces for designing anti-osteoporotic drugs. A specific binding site was discovered that allows disruption of a particular protein interaction without interfering with other related interactions. The developed inhibitor demonstrates specificity and anti-osteoporotic effects through in vitro and in vivo studies.
NATURE COMMUNICATIONS
(2022)
Article
Dentistry, Oral Surgery & Medicine
Poliana Mendes Duarte, Tamires Szeremeske Miranda, Leticia Macedo Marins, Joao Ricardo Batistao da Silva, Fernando de Souza Malta, Bruno Cesar de Vasconcelos Gurgel, Marcelo Henrique Napimoga
Summary: This study demonstrates that LiCl can enhance extraction socket repair, stimulate bone formation, and restrict TRAP levels in rats.
ORAL AND MAXILLOFACIAL SURGERY-HEIDELBERG
(2022)
Article
Endocrinology & Metabolism
Krishnakali Dasgupta, Samantha Lessard, Steven Hann, Megan E. Fowler, Alexander G. Robling, Matthew L. Warman
Summary: In this study, the researchers investigated the effects of somatic activating mutations in the PIK3CA gene on skeletal overgrowth in mice. They found that differences in phenotype between Tg(BGLAP-Cre) and Tg(DMP1-Cre) mice with PIK3CA activation may be attributed to differences in recombination rates at non-skeletal sites outside of the bone system. The development of droplet digital PCR assays allowed for the detection and quantification of recombination efficiency in various tissues, providing insights into the potential impact of off-targeting in experiments involving conditional alleles.
Article
Endocrinology & Metabolism
Eunhee Jang, Jeonghoon Ha, Ki-Hyun Baek, Moo Il Kang
Summary: The study showed that serum DKK1 levels rapidly decreased at 1 week after allo-SCT treatment and continued to increase for 48 weeks. At the same time, the RANKL/OPG ratio peaked at 4 weeks before declining. Bone mass decreased relative to the baseline at all timepoints, with the lumbar spine in female patients experiencing the largest decline.
ENDOCRINOLOGY AND METABOLISM
(2021)
Article
Biochemistry & Molecular Biology
Ragaa H. M. Salama, Safaa S. Ali, Tarek Hamdy M. Salama, Mohamed Abu Almged, Tasneem A. Alsanory, Aya A. Alsanory, Hesham Aboutaleb, Ghada M. Ezzat
Summary: This study evaluated the effects of nanopowder eggshell (NPES) on healthy and ovariectomy-induced osteoporosis rats. The results showed that NPES increased biomarkers of bone formation and bone trace elements, and alleviated osteoporosis.
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
(2023)
Article
Medicine, General & Internal
Joanna Kaminska, Marek Stopinski, Krzysztof Mucha, Michal Pac, Marek Golebiowski, Monika A. Niewczas, Leszek Paczek, Bartosz Foroncewicz
Summary: The study found that serum OPN, OC, and OPG concentrations significantly increased with the progression of renal disease, with a significant positive correlation among these proteins. OPN and OPG were significantly and positively correlated to the coronary calcium score. Serum OPG showed the strongest correlation to GFR decline and an increased risk of death, particularly in patients with CKD3-4 or ESRD.
INTERNATIONAL JOURNAL OF GENERAL MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Benedetta Ghezzi, Ludovica Parisi, Elena Calciolari, Andrea Toffoli, Biagio Matera, Simone Lumetti, Giovanni Passeri, Guido Maria Macaluso
Summary: This study investigated the effects of testosterone enanthate (TE) on MG-63 cells and found that 10 μM of TE is the critical dose that promotes bone differentiation of MG-63 cells in vitro.
Article
Endocrinology & Metabolism
Henry H. Moon, Katrina L. Clines, Patrick J. O'Day, Basel M. Al-Barghouthi, Emily A. Farber, Charles R. Farber, Richard J. Auchus, Gregory A. Clines
Summary: Bone metastasis is a common complication in advanced prostate cancer, and reducing androgen exposure remains crucial in treatment. Research suggests that osteoblasts may serve as a novel source of androgen synthesis, highlighting a potential new target for therapy.
JOURNAL OF BONE AND MINERAL RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Runbo Li, Hirohito Kato, Chihiro Fumimoto, Yurika Nakamura, Kimihiro Yoshimura, Emika Minagawa, Keiju Omatsu, Chizuko Ogata, Yoichiro Taguchi, Makoto Umeda
Summary: This study explores the cellular responses to essential amino acid (EAA) starvation, revealing that it leads to ROS-induced DNA damage and apoptosis. Treatment with the antioxidant NAC can mitigate cellular damage. This research provides insights into the intricate interplay between nutrient deficiency, oxidative stress, and programmed cell death.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Nutrition & Dietetics
Theresa L. Powell, Kenneth Barentsen, Owen Vaughan, Charis Uhlson, Karin Zemski Berry, Kathryn Erickson, Kelsey Faer, Stephanie S. Chassen, Thomas Jansson
Summary: Reducing the expression of the LPC-DHA transporter MFSD2a specifically in the placenta resulted in reduced fetal brain weight and lower phospholipid DHA content in the fetal brain. These findings provide mechanistic evidence of the critical role of placental MFSD2a in maternal-fetal transfer of LPC-DHA for brain growth.
Article
Dentistry, Oral Surgery & Medicine
Kerstin M. Galler, Eva-Maria Gratz, Matthias Widbiller, Wolfgang Buchalla, Helge Knuttel
Summary: This scoping review systematically explores the cellular and molecular processes of trauma-associated root resorption, revealing a lack of discussion on the pathophysiological mechanisms in the literature. Further research is needed to address these gaps.
Article
Dentistry, Oral Surgery & Medicine
Tayse Caroline Cunha de Medeiros, Alessandra Areas e Souza, Rodolfo Coelho Prates, Iain Chapple, Joao Paulo Steffens
Summary: This study found an association between systemic non-communicable diseases and tooth loss in Brazilian adults. Risk factors such as diabetes, smoking, and a cariogenic diet were significantly related to tooth loss. Regular consumption of vegetables, fruits, and beef, moderate alcohol intake, and physical exercise were associated with a lower risk of tooth loss. Obesity and other diseases may also increase the risk of tooth loss in individuals with diabetes.
JOURNAL OF PERIODONTOLOGY
(2022)
Article
Dentistry, Oral Surgery & Medicine
Fernanda Castanheira Goncalves, Bruno Arruda Mascaro, Cassio Rocha Scardueli, Guilherme Jose Pimentel Lopes de Oliveira, Luis Carlos Spolidorio, Rosemary Adriana Chierici Marcantonio
Summary: This study evaluated the effect of strontium ranelate on post-extraction socket healing in rats treated with bisphosphonates. The use of strontium ranelate showed subtle positive effects on connective tissue organization, but had limited impact on bone necrosis and inflammatory infiltration.
Article
Cell Biology
Vinicius de Paiva Goncalves, Joao Paulo Steffens, Carlos Rossa Junior, Luis Carlos Spolidorio
Summary: This study aimed to evaluate the effects of testosterone depletion and supraphysiological testosterone supplementation on early phase wound healing in animals. The results showed that supraphysiological testosterone supplementation improved wound healing by enhancing angiogenesis and reducing wound contraction.
INFLAMMATION RESEARCH
(2022)
Article
Oncology
Marcell Costa de Medeiros, Min Liu, Rajat Banerjee, Emily Bellile, Nisha J. D'Silva, Carlos Rossa Jr
Summary: The study found that galanin secreted by head and neck squamous cell carcinoma (HNSCC) cells exhibits immune-suppressive and pro-tumoral effects. Galanin decreases cell proliferation and expression of pro-inflammatory cytokines, while increasing apoptosis and expression of pro-tumoral cytokines/growth factors. It also suppresses T cell activation and anti-tumoral phenotypes. High expression of galanin is associated with reduced overall survival in HNSCC patients.
Article
Biochemistry & Molecular Biology
Vinicius de Paiva Goncalves, Marta Liliana Musskopf, Angeliz Rivera-Concepcion, Christina Yu, Sing Wai Wong, Stephen A. Tuin, Yizu Jiao, Cristiano Susin, Luis Carlos Spolidorio, Patricia Almeida Miguez
Summary: This study showed that hesperidin impaired osteoclastogenesis in vitro, but high-dose dietary hesperidin increased osteoclast numbers and promoted inflammation-induced alveolar bone loss in vivo. However, hesperidin at a dose of 500 mg/kg exhibited a bone-sparing effect on skeletal bones under physiological conditions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Pharmacology & Pharmacy
Cindy Grace Perez-Pacheco, Natalie Aparecida Rodrigues Fernandes, Angelo Constantino Camilli, Danilo Paschoal Ferrarezi, Amanda Favoreto Silva, Mayara Cristina Zunareli, Camila Fernanda Amantino, Fernando Lucas Primo, Morgana Rodrigues Guimaraes-Stabilli, Carlos Rossa Junior
Summary: The aim of this study was to assess the influence of local application of curcumin-loaded nanoparticles on periodontal repair. The results demonstrate that local administration of curcumin-loaded nanoparticles enhanced tissue repair in an experimental model of periodontal repair, promoting bone repair and increasing collagen content.
NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY
(2023)
Article
Dentistry, Oral Surgery & Medicine
Alvaro Formoso Pelegrin, Vinicius de Paiva Goncalves, Jhonatan de Souza Carvalho, Denise Madalena Palomari Spolidorio, Luis Carlos Spolidorio
Summary: This study aimed to evaluate the inflammatory profile and resolution of inflammation in ligature-induced periodontal inflammation in rats with depletion and/or supraphysiological testosterone replacement. Results showed that supraphysiological testosterone treatment stimulated gingival tissue repair during ligature-induced periodontitis, and this may be related to an anti-inflammatory and pro-resolutive mechanism resulting from the modulatory effect on PGE2 and IL-10.
ARCHIVES OF ORAL BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Laura Andrea Gonzalez Maldonado, Camyla Rodrigues Nascimento, Natalie Aparecida Rodrigues Fernandes, Ana Lidia Pinheiro Silva, Nisha J. D'Silva, Carlos Rossa Jr
Summary: In oral squamous cell carcinoma, macrophages in the tumor microenvironment play a significant role in prognosis and disease survival. This study focuses on the effects of tumor cell-secreted TGF-beta on macrophage phenotype and macrophage-facilitated tumor invasion. The results show that blocking the effects of TGF-beta can affect macrophage phenotype and impact tumor cell invasion and chemotaxis differently.
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
(2022)
Article
Oncology
Mauricio Rocha Dourado, Amr Elseragy, Bruno Cesar da Costa, Fabio Haach Teo, Gustavo Narvaes Guimaraes, Renato Assis Machado, Maija Risteli, Wafa Wahbi, Clarissa Araujo Gurgel Rocha, Livia Maris Ribeiro Paranaiba, Wilfredo Alejandro Gonzalez-Arriagada, Sabrina Daniela da Silva, Ana Lucia Carrinho Ayroza Rangel, Marcelo Rocha Marques, Carlos Rossa Junior, Tuula Salo, Ricardo D. Coletta
Summary: In this study, the expression and impact of stress induced phosphoprotein 1 (STIP1) in oral squamous cell carcinoma (OSCC) were investigated. It was found that STIP1 was highly expressed in OSCC, and its overexpression was associated with shorter survival and higher risk of recurrence. Knockdown of STIP1 reduced proliferation, migration, and invasion of tumor cells, and decreased the number of metastases in a zebrafish model. Furthermore, STIP1 was inversely expressed with miR-218-5p, and transfection of miR-218-5p mimics decreased STIP1 levels and inhibited tumor cell proliferation, migration, and invasion.
FRONTIERS IN ONCOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Camyla Rodrigues Nascimento, Natalie Ap Rodrigues Fernandes, Laura Andrea Gonzalez Maldonado, Carlos Rossa Junior
Summary: Understanding macrophage biology is important for understanding various biological processes and developing new immunomodulatory strategies. This study compared the response of macrophages derived from THP-1 and U937 cells to different polarizing conditions, and found that they have different tendencies towards M1 and M2 phenotypes, with THP-1-derived macrophages being more responsive to M1 stimuli.
BIOCHEMISTRY AND BIOPHYSICS REPORTS
(2022)
Article
Biochemistry & Molecular Biology
Iolanda Augusta Fernandes de Matos, Natalie Aparecida Rodrigues Fernandes, Giovani Cirelli, Mariely Araujo de Godoi, Leticia Ribeiro de Assis, Luis Octavio Regasini, Carlos Rossa Junior, Morgana Rodrigues Guimaraes-Stabili
Summary: In this in vitro study, a newly synthesized chalcone T4 was found to inhibit osteoclast differentiation and activity and stimulate osteogenesis, indicating a promising therapeutic potential in osteolytic diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Dentistry, Oral Surgery & Medicine
Elisandra Marcia Rodrigues, Kennia Scapin Viola, Laura Gonzalez Maldonado, Carlos Rossa-Junior, Juliane Maria Guerreiro-Tanomaru, Mario Tanomaru-Filho
Summary: Modified formulations of calcium silicate repair materials with additives have been developed to enhance handling, consistency, biocompatibility, and bioactivity. In this study, the effects of these modified materials on human osteoblastic cells were evaluated. The results showed that the modified cements did not have cytotoxic effects and promoted cell viability, gene expression, and mineralization processes.
BRAZILIAN ORAL RESEARCH
(2022)
Article
Biology
Rafael Scaf de Molon, Erica Dorigatti de Avila, Joni Augusto Cirelli, Joao Paulo Steffens
Summary: The periodontium is a group of structures that surround the teeth and aim to protect and support them, but are often challenged by subclinical inflammation caused by microbiota. Periodontitis is a prevalent inflammatory and destructive disease that results from complex host-parasite interactions. Periodontal research has the potential to contribute to various basic science specialties such as cell and molecular biology, microbiology, immunology, endocrinology, and rheumatology.