Article
Oncology
Zhenlin Yang, He Tian, Lin Li, Chao Li, Jiachen Xu, Fenglong Bie, Ying Chen, Yanhua Tian, Guangyu Bai, Yue Peng, Junhui Yang, Tao Fan, Chu Xiao, Wenchao Liu, Lei Liu, Renda Li, Sijin Sun, Bo Zheng, Fengwei Tan, Jianming Ying, Chunxiang Li, Shugeng Gao, Jie He
Summary: By using TTF-1 and P40 immunohistochemistry staining, 56 patients with pulmonary sarcomatoid carcinoma (PSC) were categorized into three subgroups with distinct molecular features, showing significant differences in genomic alteration spectrum and providing evidence for refined stratification in the treatment of PSC.
INTERNATIONAL JOURNAL OF CANCER
(2022)
Article
Pathology
Minhua Wang, Peter P. Chen, Guoping Cai
Summary: This study examined the expression of GATA3 in primary lung carcinomas and its correlation with tumor histology and routine immunostains. Tissue microarrays were used to analyze 184 non-small cell carcinomas and assess the staining of GATA3, p40, TTF-1, and napsin A. The study found that GATA3 expression occurs in a small but significant proportion of primary lung carcinomas, with higher levels of expression observed in more poorly differentiated subtypes.
Article
Biotechnology & Applied Microbiology
Melanie Reschke, Jenna R. DiRito, David Stern, Wesley Day, Natalie Plebanek, Matthew Harris, Sarah A. Hosgood, Michael L. Nicholson, Danielle J. Haakinson, Xuchen Zhang, Wajahat Z. Mehal, Xinshou Ouyang, Jordan S. Pober, William Mark Saltzman, Gregory T. Tietjen
Summary: Automated image analysis tools are needed in preclinical research to provide robust and rapid quantification of histologic samples from paraffin-embedded or cryopreserved tissues, addressing limitations and subjectivity associated with manual assessments. The development of such tools enables thorough and reliable characterization of histological samples, improving rigor and reproducibility in tissue-based analyses.
BIOENGINEERING & TRANSLATIONAL MEDICINE
(2022)
Article
Medicine, General & Internal
Tao Sun, Hanwen Zhang, Wei Gao, Qifeng Yang
Summary: The study assessed the sensitivity, specificity, and experience of freehanded CNB in outpatient settings for breast cancer diagnosis. Results showed that taking 2 tissue strips by CNB was as reliable as taking six strips in distinguishing malignancy from benignancy without the aid of US. Four tissue strips obtained by CNB showed good accuracy in assessing ER, PR, HER2 status, and tumor grade compared to surgical specimens. Two tissue strips obtained by CNB were accurate in differentiating malignancy from benignancy, but at least 4 strips are recommended for overall conformity of pathological biomarkers.
Article
Pathology
Arnault Tauziede-Espariat, Sophie Huybrechts, Emilie Indersie, Christelle Dufour, Stephanie Puget, Anais Chivet, Alexandre Roux, Melanie Pages, Albane Gareton, Fabrice Chretien, Emmanuele Lechapt, Olivier Ayrault, Pascale Varlet
Summary: Medulloblastomas (MBs) are the most frequent malignant brain tumors in children, classified into four histopathologic variants and four genetic subgroups. Immunohistochemistry with four antibodies showed high concordance with DNA-methylation profiling in subgrouping MBs, highlighting the utility of immunostaining as a reliable predictive marker. A combination of two antibodies (YAP1 and OTX2) successfully characterized the majority of cases, extending the molecular data of rare morphologic variants of MBs with melanotic/myogenic differentiations.
AMERICAN JOURNAL OF SURGICAL PATHOLOGY
(2021)
Article
Endocrinology & Metabolism
Meejeong Kim, Sora Jeon, Chan Kwon Jung
Summary: RAS Q61R IHC can be used as a diagnostic test for NIFTP/malignancy in CNB category III/IV results, and combining it with the histologic parameter of nuclear atypia can further stratify the risk level.
ENDOCRINE PATHOLOGY
(2023)
Article
Oncology
Jun Zhang, Peng Jiang, Chunxia Gong, Wei Kong, Yuan Tu, Yuzhen Huang, Ying Liu
Summary: The study aimed to explore the consistency of P53 immunohistochemical expression between preoperative biopsy and final pathology in endometrial cancer (EC), and to predict the prognosis of patients based on the 4-tier P53 expression and classic clinicopathological parameters. The consistency of P53 expression between preoperative biopsy and final pathology was 83.8%, with a kappa coefficient of 0.624. The 4-tier P53 expression was found to be an independent influencing factor for recurrence and death, and nomogram models were successfully established to predict the prognosis of patients.
FRONTIERS IN ONCOLOGY
(2023)
Article
Oncology
Hani Saghir, Srinivas Veerla, Martin Malmberg, Lisa Ryden, Anna Ehinger, Lao H. Saal, Johan Vallon-Christersson, Ake Borg, Cecilia Hegardt, Christer Larsson, Alaa Haidar, Ingrid Hedenfalk, Niklas Loman, Siker Kimbung
Summary: This study aimed to assess the reliability of using CNB for preoperative tumor characterization in early breast cancer. Results showed moderate to very good correlation between IHC and GEX assessment in paired CNB and surgical specimens, with some discrepancies observed in Ki67 and ER across different techniques, potentially impacting treatment decisions and patient outcomes.
Article
Oncology
Esther Visser, Sylvia A. A. M. Genet, Remco P. P. A. de Kock, Ben E. E. M. van den Borne, Maggy Youssef-El Soud, Huub N. A. Belderbos, Gerben Stege, Marleen E. A. de Saegher, Susan C. Van't Westeinde, Luc Brunsveld, Maarten A. C. Broeren, Daan Van de Kerkhof, Birgit A. L. M. Deiman, Federica Eduati, Volkher Scharnhorst
Summary: This study aimed to evaluate the performance of liquid biopsy-based decision-support algorithms in diagnosing lung cancer and subtyping it into small-cell and non-small-cell lung cancer. The study found that using protein tumor markers and circulating tumor DNA could improve the accuracy of lung cancer diagnosis. These algorithms are particularly important for patients who cannot undergo tissue biopsies or have inconclusive results.
Article
Oncology
Slavica Janeva, Toshima Z. Parris, Salmir Nasic, Shahin De Lara, Karolina Larsson, Riccardo A. Audisio, Roger Olofsson Bagge, Aniko Kovacs
Summary: The comparison between STRAT4 and IHC/SISH showed discrepancies in BC patients, potentially resulting in more adjuvant treatment.
Article
Gastroenterology & Hepatology
Kazuyuki Matsumoto, Toshiaki Ohara, Masayoshi Fujisawa, Akinobu Takaki, Masahiro Takahara, Hironari Kato, Ryuichi Yoshida, Yuzo Umeda, Takahito Yagi, Akihiro Matsukawa, Hiroyuki Okada
Summary: This study evaluated whether biopsy specimens could predict the positive status of PD-L1 in surgically resected tissue. The results showed a substantial positive and high negative concordance rates between biopsy samples and surgically resected tissues in terms of PD-L1 expression.
JOURNAL OF GASTROINTESTINAL SURGERY
(2022)
Article
Oncology
Cor J. Ravensbergen, Matthew Kuruc, Meaghan Polack, Stijn Crobach, Hein Putter, Hans Gelderblom, Devjit Roy, Rob A. E. M. Tollenaar, Wilma E. Mesker
Summary: Liquid biopsy is a novel and minimally invasive method for tumor sampling, providing a comprehensive understanding of the molecular tumor landscape. However, the current liquid biopsy analytes mainly focus on malignant cells without considering the tumor microenvironment. In this study, the Stroma Liquid Biopsy(TM) (SLB) proteomics panel, which contains proteins related to key stromal pathways in cancer, was explored and characterized using an in-silico transcriptomics approach in colon cancer. The expression of SLB genes was found to be significantly enriched in tumors with high histologic stromal content, and a prognostic gene signature ratio was identified. The findings highlight the importance of considering the tumor microenvironment in liquid biopsy and provide valuable prognostic information.
Article
Medicine, General & Internal
Mohammed S. I. Mansour, Kim Hejny, Felicia Johansson, Joudy Mufti, Ante Vidis, Ulrich Mager, Annika Dejmek, Tomas Seidal, Hans Brunnstroem
Summary: The study found a good correlation between PD-L1 expression in cytology specimens and histological specimens, with different types of biopsies not significantly impacting the concordance. A high amount of tumor cells in biopsies may enhance the cyto-histological PD-L1 concordance.
Article
Medical Laboratory Technology
Elizabeth M. Jacobi, Gene Landon, Russell R. Broaddus, Sinchita Roy-Chowdhuri
Summary: This study investigated the feasibility of using cell block preparations for MMR IPOX evaluation and found a high concordance of MMR IPOX testing between cytologic effusion specimens and surgical pathology specimens. However, some cases exhibited limitations such as low tumor cell numbers and unclear staining in individual cells.
ARCHIVES OF PATHOLOGY & LABORATORY MEDICINE
(2021)
Article
Pathology
Michel Kmeid, Mahmoud Aldyab, Rupinder Brar, Hwajeong Lee
Summary: In patients treated with RTX, tissue B-cell depletion does not seem to be the main cause of inflammatory pathology in the GI tract. However, a minor subset of patients develop histologic evidence of potential RTX-induced effect, notably in the form of LCPI.
AMERICAN JOURNAL OF CLINICAL PATHOLOGY
(2022)