Journal
HISTOCHEMISTRY AND CELL BIOLOGY
Volume 139, Issue 4, Pages 535-548Publisher
SPRINGER
DOI: 10.1007/s00418-012-1044-y
Keywords
Sperm; Acrosome; Liprin alpha 3; Estrogen; Testis and epididymis
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Funding
- Department of Science and Technology, New Delhi
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Liprin alpha 3 was reported for the first time using sperm proteomics. Present study reports its localization on sperm and immunochemical characterization. Liprin alpha 3 is identified as a 133 kDa protein in testis and epididymal protein extracts. In testis, immunohistochemical localization was seen in pachytenes, diplotenes, round spermatids whereas it was localized in the epithelial cells and luminal sperm in all the three regions of epididymis. Protein was localized in acrosome of rat sperm, which was further confirmed by sequential treatment of sperm with hypertonic solution. In the spermatogenic cells the protein was found to be located in developing acrosome as evident by its co-localization with Golgi marker. Protein was found to be developmentally regulated. In silico analysis of Liprin alpha 3 revealed presence of the estrogen responsive elements upstream to initiation site and its regulation by estrogen was experimentally validated using a tamoxifen treated rat model. Western blot analysis of epididymosomes showed the presence of Liprin alpha 3, indicating its involvement in trafficking of vesicle. The protein expression was seen in both mouse and human sperm indicating conserved nature and a probable role in acrosome reaction.
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