Journal
HISTOCHEMISTRY AND CELL BIOLOGY
Volume 135, Issue 2, Pages 173-181Publisher
SPRINGER
DOI: 10.1007/s00418-011-0777-3
Keywords
PI3K/Akt; SREBP-1; TGF-beta 1; Lipogenesis; Extracellular matrix; High glucose
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Funding
- Public Health Department of Hebei Province of China [20090050]
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Previous studies have shown that high glucose stimulates renal SREBP-1 gene expression and increases renal tubular cells lipid metabolism, however, the mechanisms remain elusive. In the present study we demonstrated that PI3K/Akt pathway was activated in human renal proximal tubular cell line (HKC) exposed to high glucose accompanied with up-regulation of SREBP-1, TGF-beta 1, lipid droplets deposits and extracellular matrix production. Inhibition of PI3K/Akt pathway by chemical LY294002 or specific short hairpin RNA (shRNA) vector prevented SREBP-1 and TGF-beta 1 up-regulation, as well as ameliorated HKC cells lipogenesis and extracellular matrix accumulation. These findings indicate that PI3K/Akt pathway potentially mediates high glucose-induced lipogenesis and extracellular matrix accumulation in HKC cells.
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