Journal
ANNALS OF PLASTIC SURGERY
Volume 74, Issue 3, Pages 371-375Publisher
LIPPINCOTT WILLIAMS & WILKINS
DOI: 10.1097/SAP.0000000000000439
Keywords
prevascular; scaffold-free; xenograft; anastomosis; vascularization; skeletal muscle; satellite cell; Pax-7; MyoD
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Funding
- NIH [R01 DE019355, R01 HL080168]
- NSF EPSCoR RII [EPS-090375]
- Office of Integrative Activities
- Office Of The Director [1317771] Funding Source: National Science Foundation
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To evaluate the anastomotic potential of prevascular tissue constructs generated from scaffold-free self-assembly of human endothelial and fibroblast cells, tissue constructs were implanted into athymic mice and immune-competent rats. Analysis of xenografts placed into hind limb muscle defects showed vascular anastomotic activity by 3 days after implantation and persisting for 2 weeks. Integration of the implanted prevascular tissue constructs with the host circulatory system was evident from presence of red blood cells in the implant as early as 3 days after implantation. Additionally, analysis of 3-day xenografts in the rat model showed activation of skeletal muscle satellite cells based on Pax-7 and MyoD expressions. We conclude that prevascular tissue constructs generated from scaffold-free self-assembly of human endothelial and fibroblast cells are a promising tool to provide both vascular supply and satellite cell activation toward the resolution of skeletal muscle injury.
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