Article
Biochemistry & Molecular Biology
Hyun-Jung An, Cheol-Jung Lee, Ga-Eun Lee, Youngwon Choi, Dohyun Jeung, Weidong Chen, Hye Suk Lee, Han Chang Kang, Joo Young Lee, Dae Joon Kim, Jin-Sung Choi, Eun Suh Cho, Jong-Soon Choi, Yong-Yeon Cho
Summary: FBXW7 regulates ERK3 stability by promoting its ubiquitination and degradation, thereby suppressing lung cancer cell proliferation.
EXPERIMENTAL AND MOLECULAR MEDICINE
(2022)
Article
Oncology
Yongjie Zhang, Juan Li, Bo Wang, Ting Chen, Yun Chen, Wen Ma
Summary: LDH-A can promote the proliferation of dMMR and pMMR CRC cells by positively regulating MMR proteins, LDH-A inhibition may improve the efficacy of PD-1 blockade, a significant correlation between LDH-A and dMMR status was observed in 186 CRC specimens, LDH-A and dMMR double-low patients showed better disease-free survival.
Article
Chemistry, Multidisciplinary
Debao Fang, Hao Hu, Kailiang Zhao, Aman Xu, Changjun Yu, Yong Zhu, Ning Yu, Bo Yao, Suyun Tang, Xianning Wu, Yide Mei
Summary: MLF2 is a new negative regulator of p53 that interacts with both p53 and USP7. It inhibits USP7-mediated deubiquitination of p53, leading to p53 destabilization and promoting colorectal cancer. Clinically, MLF2 is elevated in colorectal cancer and its high expression is associated with poor prognosis.
Review
Oncology
Chen Kai Huang, Ying Sun, Lei Lv, Yong Ping
Summary: This passage introduces the role of alpha-Enolase (ENO1) as a glycolytic enzyme in cancer. Additionally, it acts as a plasminogen receptor on the cell surface, promoting cancer invasion and metastasis. The overexpression of ENO1 and its localization on the tumor surface make it a prognostic and diagnostic cancer biomarker as well as a therapeutic target.
MOLECULAR THERAPY-ONCOLYTICS
(2022)
Article
Gastroenterology & Hepatology
Jie Hong, Fangfang Guo, Shi-Yuan Lu, Chaoqin Shen, Dan Ma, Xinyu Zhang, Yile Xie, Tingting Yan, TaChung Yu, Tiantian Sun, Yun Qian, Ming Zhong, Jinxian Chen, Yanshen Peng, Cheng Wang, Xiang Zhou, Jianjun Liu, Qiang Liu, Xiong Ma, Ying-Xuan Chen, Haoyan Chen, Jing-Yuan Fang
Summary: The study revealed a positive correlation between Fusobacterium nucleatum and high glucose metabolism in patients with colorectal cancer (CRC), suggesting a potential role of gut microbiota in CRC development. Mechanistically, Fusobacterium nucleatum activated the lncRNA ENO1-IT1 pathway to regulate CRC cell glucose metabolism and biological functions. Targeting the ENO1 pathway may have therapeutic implications for CRC patients with elevated Fusobacterium nucleatum levels.
Article
Biochemistry & Molecular Biology
Wei Wang, Xiang-Dong Bai, Kun Chen, Xiao-Yue Zhang, Chen-Rui Gu, Jing Jiang, Chuan-Ping Yang, Gui-Feng Liu
Summary: This study found that the interference expression of the PsnWRKY70 gene can enhance the tolerance of poplar to saline-alkali stress and has a positive effect on the growth and alkali damage of this tree species. Transcriptome analysis showed that the interference expression of the PsnWRKY70 gene up-regulated multiple genes related to cell wall organization or biogenesis pathway, which play an important role in NaHCO3 stress.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Oncology
Wei Shang, Chuanwang Yan, Ran Liu, Lili Chen, Dongdong Cheng, Liang Hao, Wenguang Yuan, Jingbo Chen, Hui Yang
Summary: The study suggests that FBXW7 mutation/low expression is associated with advanced T stage and lymph node metastasis in CRC patients, and it may serve as a prognostic biomarker for overall survival in CRC.
Article
Plant Sciences
Jing Guo, Yaqiong Wu, Tongli Wang, Yue Xin, Guibin Wang, Qi Zhou, Li-An Xu
Summary: Flavonoids in ginkgo are important secondary metabolites with extensive pharmacological functions. However, the biosynthesis of ginkgo flavonols is not well understood. In this study, the GbFLSa gene was cloned and found to potentially negatively regulate the biosynthesis of proanthocyanins. This study is significant for elucidating the role of GbFLSa in ginkgo metabolism and the molecular mechanism of flavonoid biosynthesis.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Debora Cabot, Sonia Brun, Noelia Paco, Mireia M. Ginesta, Nuria Gendrau-Sanclemente, Baraa Abuasaker, Triana Ruiz-Farina, Carles Barcelo, Miriam Cuatrecasas, Marta Bosch, Carles Rentero, Gabriel Pons, Josep M. Estanyol, Gabriel Capella, Montserrat Jaumot, Neus Agell
Summary: Research shows that the phosphorylation status of oncogenic KRAS is crucial for maintaining the malignant properties and tissue structure of colorectal cancer cells, as well as playing a key role in tumor growth in mice and gene expression regulation.
Article
Cell Biology
Sha Zhou, Jianhong Peng, Liuniu Xiao, Caixia Zhou, Yujing Fang, Qingjian Ou, Jiayi Qin, Mengzhong Liu, Zhizhong Pan, Zhenlin Hou
Summary: TRIM25 is identified as an epigenetic regulator of oxaliplatin resistance in colorectal cancer patients, affecting disease-free survival and recurrence rates. Its overexpression promotes stem cell properties in CRC cells and resistance to chemotherapy. Targeting TRIM25 may be a potential strategy for managing OXA resistance in clinical practice.
CELL DEATH & DISEASE
(2021)
Article
Oncology
Jinrong Gu, Kaiqiang Zhong, Longgang Wang, Haishun Ni, Yirui Zhao, Xuchao Wang, Yizhou Yao, Linhua Jiang, Bin Wang, Xinguo Zhu
Summary: This study identified that ENO1 regulates 5-FU resistance via the EMT pathway and may be a novel target in the prevention and treatment of 5-FU resistant CRC.
FRONTIERS IN ONCOLOGY
(2022)
Article
Cell Biology
Mi-Jeong Kim, Yoon Min, Soo-Kyung Jeong, Juhee Son, Ji Young Kim, Ji Su Lee, Duk-Hwan Kim, Joo Sang Lee, Eunyoung Chun, Ki-Young Lee
Summary: The study reveals that USP15 is significantly downregulated in lung cancer patients, and its knockout leads to increased migration, invasion, and autophagy induction in lung cancer cells. USP15 interacts with BECN1 and deubiquitinates it, thereby attenuating autophagy induction. Additionally, the expression of USP15 is associated with the upregulation of certain oncogenes and downregulation of tumor suppressor genes in lung cancer patients.
CELL DEATH & DISEASE
(2022)
Article
Cell Biology
Kangyue Sun, Lu Chen, Yiwen Li, Bing Huang, Qun Yan, Changjie Wu, Qiuhua Lai, Yuxin Fang, Jianqun Cai, Yongfeng Liu, Junsheng Chen, Xinke Wang, Yuxuan Zhu, Shuyu Dong, Jieyu Tan, Aimin Li, Side Liu, Yue Zhang
Summary: miR-17-5p is involved in CRC proliferation and metastasis, and m(6)A modification plays a role in RNA modification. This study found that miR-17-5p overexpression leads to chemotherapy resistance in CRC by affecting mitochondrial homeostasis. METTL14 regulates miR-17-5p expression through m(6)A mRNA methylation, and the METTL14/miR-17-5p/MFN2 signaling pathway is critical for 5-FU chemoresistance in CRC.
CELL DEATH & DISEASE
(2023)
Article
Cell Biology
Yuanzhou Zhang, Shunshun Liang, Bowen Xiao, Jingying Hu, Yechun Pang, Yuling Liu, Juan Yang, Junpin Ao, Lin Wei, Xiaoying Luo
Summary: This study shows that ErbB3/EGFR receptor tyrosine kinase activity is increased in colorectal cancer cells, and miR-323a-3p can directly target both ErbB3 and EGFR, promoting apoptosis in CRC cells. Furthermore, miR-323a-3p acts as a multi-ErbBs inhibitor, enhancing sensitivity to gefitinib and preventing acquired resistance.
CELL DEATH & DISEASE
(2022)
Article
Biochemistry & Molecular Biology
Jia-Yi Hou, Jing Cao, Li-Juan Gao, Fu-Peng Zhang, Jing Shen, Lan Zhou, Jian-Yun Shi, Yan-Lin Feng, Zi Yan, De-Ping Wang, Ji-Min Cao
Summary: The study reveals that lysine crotonylation (Kcr) of enolase (ENO1) is significantly elevated in human colorectal cancer (CRC) tissues. CREB-binding protein (CBP) acts as a crotonyltransferase of ENO1, while SIRT2 is involved in the decrotonylation of ENO1. Crotonylation of ENO1 at K420 promotes the growth, migration, and invasion of CRC cells by enhancing ENO1 activity and regulating the expression of tumor-associated genes.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Oncology
A. W. Mao, H. Barck, J. Young, A. Paley, J-H Mao, H. Chang
Summary: The study identified a 15-microbe prognostic signature for breast cancer patients by analyzing TCGA BC microbiome data, which showed good predictive efficacy in patient survival and progression-free survival. The microbial abundance prognostic scoring model was found to be an independent prognostic factor for overall survival and progression-free survival, outperforming previously published gene signatures.
CLINICAL & TRANSLATIONAL ONCOLOGY
(2022)
Article
Oncology
Ines Guix, Qi Liu, Miquel Angel Pujana, Patrick Ha, Josep Piulats, Isabel Linares, Ferran Guedea, Jian-Hua Mao, Ann Lazar, Jocelyn Chapman, Sue S. Yom, Alan Ashworth, Mary Helen Barcellos-Hoff
Summary: This study confirms the anticorrelation between TGF beta signaling and alt-EJ gene expression signatures, as well as their predictive value for response to genotoxic therapy. The findings validate the role of TGF beta as a key mediator of DNA repair and demonstrate the feasibility of using gene expression to assess TGF beta signaling and DNA repair.
CLINICAL CANCER RESEARCH
(2022)
Article
Multidisciplinary Sciences
Jianhui Bin, Lieselotte Obst-Huebl, Jian-Hua Mao, Kei Nakamura, Laura D. Geulig, Hang Chang, Qing Ji, Li He, Jared De Chant, Zachary Kober, Anthony J. Gonsalves, Stepan Bulanov, Susan E. Celniker, Carl B. Schroeder, Cameron G. R. Geddes, Eric Esarey, Blake A. Simmons, Thomas Schenkel, Eleanor A. Blakely, Sven Steinke, Antoine M. Snijders
Summary: Radiotherapy is the standard treatment for over 50% of cancer patients, and the use of FLASH-RT may provide additional benefits. Researchers have developed an experimental platform that delivers ultra-high dose rates of proton pulses to biological samples. Preliminary findings show that normal cells have higher survival rates compared to tumor cells at high dose rates.
SCIENTIFIC REPORTS
(2022)
Review
Oncology
Roberto Corchado-Cobos, Natalia Garcia-Sancha, Marina Mendiburu-Elicabe, Aurora Gomez-Vecino, Alejandro Jimenez-Navas, Manuel Jesus Perez-Baena, Marina Holgado-Madruga, Jian-Hua Mao, Javier Canueto, Sonia Castillo-Lluva, Jesus Perez-Losada
Summary: Metabolic changes in tumor cells and their interactions with stromal cells play a crucial role in tumor growth and progression. Understanding these metabolic changes can help identify new treatment strategies. Breast cancer serves as a useful example to illustrate the metabolic alterations in cancer.
Article
Oncology
Hang Chang, Xu Yang, Jade Moore, Xiao-Ping Liu, Kuang-Yu Jen, Antoine M. Snijders, Lin Ma, William Chou, Roberto Corchado-Cobos, Natalia Garcia-Sancha, Marina Mendiburu-Elicabe, Jesus Perez-Losada, Mary Helen Barcellos-Hoff, Jian-Hua Mao
Summary: Mouse models of cancer play an important role in investigating cancer biology. This study developed a machine learning approach to identify cellular morphometric biomarkers from digital images of mammary tumors, and demonstrated their significance in predicting survival and metastasis. The findings suggest that this approach can provide unbiased biological stratification to guide patient care.
FRONTIERS IN ONCOLOGY
(2022)
Article
Immunology
Dafa Ding, Huijuan Yong, Na You, Wei Lu, Xu Yang, Xiaolong Ye, Yayun Wang, Tingting Cai, Xiaoling Zheng, Hui Chen, Bota Cui, Faming Zhang, Xingyin Liu, Jian-Hua Mao, Yibing Lu, Hang Chang
Summary: This study found that fecal microbiota transplantation (FMT) therapy can improve symptoms and metabolic parameters in patients with type 2 diabetes mellitus (T2DM). Out of 17 patients, 11 showed significant improvement. Additionally, the presence of certain microbial species can predict the clinical response to FMT treatment.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Cell Biology
Binbin Yuan, Chengfei Jiang, Lingyan Chen, Lihui Wen, Jinlong Cui, Min Chen, Shu Zhang, Lin Zhou, Yimeng Cai, Jian-Hua Mao, Xiaoping Zou, Bo Hang, Pin Wang
Summary: Through evaluating the DNA repair gene signature in gastric cancer patients, this study found that patients with high scores had better therapeutic outcomes with immune checkpoint blockade therapy. These high DRGS score patients exhibited higher levels of anti-tumor lymphocyte infiltration, tumor mutation burden, PD-L1 expression, and longer overall survival.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Oncology
Xiao-Ping Liu, Xiaoqing Jin, Saman Seyed Ahmadian, Xu Yang, Su-Fang Tian, Yu-Xiang Cai, Kuldeep Chawla, Antoine M. Snijders, Yankai Xia, Paul J. van Diest, William A. Weiss, Jian-Hua Mao, Zhi-Qiang Li, Hannes Vogel, Hang Chang
Summary: By developing and validating cellular morphometric subtypes (CMS), personalized diagnosis and therapy for LGG patients can be achieved. These subtypes can predict patient prognosis and treatment response based on molecular alterations and immune microenvironment, and also have translational impact on glioblastoma.
Article
Veterinary Sciences
Libette J. Roman, Antoine M. Snijders, Hang Chang, Jian-Hua Mao, Kristina J. A. Jones, Gregory W. Lawson
Summary: This study examines the effect of different barrier practices on the fecal microbiome of breeding C57Bl/6J mice. The results show that differences in barrier husbandry practices can affect fecal microbiome divergence in both the parental and filial generations of different breeding colonies.
JOURNAL OF THE AMERICAN ASSOCIATION FOR LABORATORY ANIMAL SCIENCE
(2023)
Article
Environmental Sciences
Hui Yang, Xinzhi Wang, Pin Wang, Li He, Suzyann F. Schick, Peyton Jacob, Neal Benowitz, Lara A. Gundel, Chi Zhu, Yankai Xia, Jamie L. Inman, Hang Chang, Antoine M. Snijders, Jian-Hua Mao, Bo Hang
Summary: Increasing evidence suggests that thirdhand smoke (THS) exposure has adverse health effects and may increase cancer risk. This study used the Collaborative Cross (CC) mouse population-based model system to assess the effects of THS exposure on cancer risk and found an increased incidence and burden of tumors in THS-treated mice. Lung and liver tissues were particularly susceptible to tumorigenesis after THS exposure. The study also highlighted the importance of host genetic background in individual susceptibility to THS-induced tumorigenesis and emphasized the need to consider genetic factors when determining cancer risk in humans exposed to THS.
ENVIRONMENT INTERNATIONAL
(2023)
Article
Substance Abuse
Georg E. Matt, Lydia Greiner, Rachael A. Record, Heather Wipfli, Jamie Long, Nathan G. Dodder, Eunha Hoh, Nicolas Lopez Galvez, Thomas E. Novotny, Penelope J. E. Quintana, Hugo Destaillats, Xiaochen Tang, Antoine M. Snijders, Jian-Hua Mao, Bo Hang, Suzaynn Schick, Peyton Jacob, Prue Talbot, E. Melinda Mahabee-Gittens, Ashley L. Merianos, Thomas F. Northrup, Lara Gundel, Neal L. Benowitz
Summary: Since the 1970s, policies prohibiting smoking indoors have saved numerous lives and reduced healthcare expenditure. However, these policies do not fully protect the public from the long-lasting toxic residues of tobacco smoke, known as thirdhand smoke. This article discusses the differences between secondhand and thirdhand smoke exposure and proposes four policy considerations to better protect the public and remediate thirdhand smoke.
Article
Endocrinology & Metabolism
Lijuan Chen, Lin Guo, Susu Feng, Congcong Wang, Zhicheng Cui, Sijing Wang, Qingmiao Lu, Hang Chang, Bo Hang, Antoine M. Snijders, Jian-Hua Mao, Yibing Lu, Dafa Ding
Summary: This study confirms the effectiveness of FMT in treating patients with T2DM and demonstrates its positive impact on gut microbiome, serum metabolites, intestinal epithelial barrier, inflammation, and circulating immune cells.
BMJ OPEN DIABETES RESEARCH & CARE
(2023)
Article
Cell Biology
Aurora Gomez-Vecino, Roberto Corchado-Cobos, Adrian Blanco-Gomez, Natalia Garcia-Sancha, Sonia Castillo-Lluva, Ana Martin-Garcia, Marina Mendiburu-Elicabe, Carlos Prieto, Sara Ruiz-Pinto, Guillermo Pita, Alejandro Velasco-Ruiz, Carmen Patino-Alonso, Purificacion Galindo-Villardon, Maria Linarejos Vera-Pedrosa, Jose Jalife, Jian-Hua Mao, Guillermo Macias de Plasencia, Andres Castellanos-Martin, Maria del Mar Saez-Freire, Susana Fraile-Martin, Telmo Rodrigues-Teixeira, Carmen Garcia-Macias, Julie Milena Galvis-Jimenez, Asuncion Garcia-Sanchez, Maria Isidoro-Garcia, Manuel Fuentes, Maria Begona Garcia-Cenador, Francisco Javier Garcia-Criado, Juan Luis Garcia-Hernandez, Maria Angeles Hernandez-Garcia, Juan Jesus Cruz-Hernandez, Cesar Augusto Rodriguez-Sanchez, Alejandro Martin Garcia-Sancho, Estefania Perez-Lopez, Antonio Perez-Martinez, Federico Gutierrez-Larraya, Antonio J. Carton, Jose Angel Garcia-Saenz, Ana Patino-Garcia, Miguel Martin, Teresa Alonso-Gordoa, Christof Vulsteke, Lieselot Croes, Sigrid Hatse, Thomas Van Brussel, Diether Lambrechts, Hans Wildiers, Hang Chang, Marina Holgado-Madruga, Anna Gonzalez-Neira, Pedro L. Sanchez, Jesu Perez Losada
Summary: Cardiotoxicity due to anthracyclines (CDA) affects cancer patients, and identifying individuals at risk is challenging. In this study, the authors investigated intermediate molecular phenotypes (IMPs) associated with myocardial damage to determine CDA susceptibility. By analyzing a mouse cohort treated with doxorubicin and docetaxel, they identified genetic markers linked to IMPs and CDA. These markers were also found to be associated with CDA in cancer patients, highlighting their potential as predictive factors. Additionally, genetic risk scores were generated using machine-learning regression to personalize patient management.
Article
Oncology
Xuanyu Mao, Yimeng Cai, Sarah Long, Jesus Perez-Losada, Jian-Hua Mao, Hang Chang
Summary: Increased mitotic activity is associated with the genesis and aggressiveness of many cancers. The mitotic network activity index (MNAI) constructed based on a 54-gene mitotic apparatus network has been evaluated as a potential prognostic biomarker in a pan-cancer study. The study confirmed the significant association of MNAI with overall survival, progression-free survival, and other prognostic endpoints in multiple cancer types, and also revealed its association with genetic instability and treatment response.
FRONTIERS IN ONCOLOGY
(2023)
Article
Microbiology
Kenneth H. H. Wan, Soo Park, Benjamin W. W. Booth, Ella C. C. Brydon, Joanne Eichenberger, Jamie L. L. Inman, Jian-Hua Mao, Antoine M. M. Snijders, Susan E. E. Celniker
Summary: Microbacterium sp. BDGP8 is a facultative anaerobic gram-positive bacterium belonging to the family Microbacteriaceae. Its complete genome consists of a single circular chromosome of 3,293,567 bp with a G + C content of 69.84% and two plasmids of 49,365 bp and 32,884 bp.
MICROBIOLOGY RESOURCE ANNOUNCEMENTS
(2023)
Article
Medicine, Research & Experimental
Marijn A. Scheijde-Vermeulen, Lennart A. Kester, Liset Westera, Bastiaan B. J. Tops, Friederike A. G. Meyer-Wentrup
Summary: This study aimed to evaluate the feasibility of integrating state-of-the-art sequencing techniques and flow cytometry into the diagnostic workup of pediatric lymphoma. The results showed that this integration is not only feasible but also provides additional diagnostic information.
LABORATORY INVESTIGATION
(2024)
Article
Medicine, Research & Experimental
Enrico Berrino, Sara Erika Bellomo, Anita Chesta, Paolo Detillo, Alberto Bragoni, Amedeo Gagliardi, Alessio Naccarati, Matteo Cereda, Gianluca Witel, Anna Sapino, Benedetta Bussolati, Gianni Bussolati, Caterina Marchi
Summary: Formalin-fixed paraffin-embedded (FFPE) samples are crucial for tissue-based analysis in precision medicine, but the quality of these samples can affect the reliability of sequencing data. The use of acid-deprived fixatives guarantees the highest DNA preservation and sequencing performance, enabling more complex molecular profiling of tissue samples.
LABORATORY INVESTIGATION
(2024)
Article
Medicine, Research & Experimental
Roope A. Kallionpaa, Sirkku Peltonen, Kim My Le, Eija Martikkala, Mira Jaaskelainen, Elnaz Fazeli, Pilvi Riihila, Pekka Haapaniemi, Anne Rokka, Marko Salmi, Ilmo Leivo, Juha Peltonen
Summary: This study investigated the immune microenvironment of cutaneous neurofibromas (cNFs) in patients with neurofibromatosis 1 (NF1). The results showed that cNFs have substantial populations of T cells and macrophages, which may be tumor-specific. T cell populations in cNFs were found to be different from those in the skin, and cNFs exhibited lower expression of proteins related to T cell-mediated immunity compared to the skin.
LABORATORY INVESTIGATION
(2024)