Journal
HEARING RESEARCH
Volume 244, Issue 1-2, Pages 1-6Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.heares.2008.07.007
Keywords
Laser microdissection; Human temporal bones; Mitochondrial DNA deletions
Funding
- Bloom Endowment Fund
- American Hearing Research Foundation
Ask authors/readers for more resources
Laser microdissection (LMD) has been used to isolate groups of cells and single cells from numerous tissues. in this study, we describe a technique for isolating cochlear structures and individual spiral ganglion cells from archival celloidin embedded human temporal bone sections. The specimens isolated are suitable for quantifying the mitochondrial DNA (mtDNA) common deletion (CD) within these tissues using a real time polymerase chain reaction (PCR) assay. The results presented in this manuscript demonstrate the feasibility of using this LMD technique to study the accumulation of mtDNA deletions in diseases of the ear. To our knowledge, this approach to analyzing archival human temporal bone tissues has not been previously reported. (C) 2008 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available