Journal
HEAD AND NECK-JOURNAL FOR THE SCIENCES AND SPECIALTIES OF THE HEAD AND NECK
Volume 31, Issue 12, Pages 1624-1633Publisher
WILEY
DOI: 10.1002/hed.21137
Keywords
antisense oligonucleotide; glucose transporter-1; glucose uptake; laryngeal carcinoma; therapy
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Funding
- Department of Education of Zhejiang Province [20060421]
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Background. Malignant cells show increased glucose uptake in vitro and in vivo, which is thought to be mediated by glucose transporters. In this study, we investigated the effect of plasmid-derived antisense RNA against the Glut-I gene on proliferation and glucose uptake in laryngeal carcinoma Hep-2 cells. Methods. The expression plasmids pcDNA3.1(+)-Glut-1 and pcDNA3. 1(+)-anti Glut-1 were constructed. The MTT method was used to assess cell growth inhibition. The expression of Glut-1 mRNA and protein was detected by reverse transcriptase-polymerase chain reaction and Western blotting, respectively. Results. After transfection, Glut-1 AS clearly inhibited glucose uptake and cell growth in Hep-2 cells, and we observed a decrease in the expression of Glut-1 mRNA and protein in Hep-2 cells. Conclusions. Glut-1 AS decreases glucose uptake and inhibits the proliferation of Hep-2 cells. (C) 2009 Wiley Periodicals, Inc. Head Neck 31: 1624-1633, 2009
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