Article
Limnology
Hongjia Huang, Chuqiao Gan, Jiahui Huang, Chen Zou, Hongye Li, Jiesheng Liu, Weidong Yang
Summary: Research shows that different strains of Prorocentrum donghaiense exhibit varying growth responses to the allelopathic action from Alexandrium pacificum, with some strains able to grow well despite inhibition in the short term, indicating the potential coexistence of these two algal species in the same area.
JOURNAL OF OCEANOLOGY AND LIMNOLOGY
(2021)
Article
Food Science & Technology
Marc Long, Bernd Krock, Justine Castrec, Urban Tillmann
Summary: Various species of Alexandrium can produce bioactive compounds, including uncharacterized bioactive extracellular compounds (BECs) which may have significant ecological impacts. Due to a lack of information on BECs in experimental strains, contrasting results in literature make it impossible to distinguish between the effects of PSTs and BECs. Further research is needed to identify the nature of Alexandrium BECs and understand their potential ecological importance and collateral damage to marine organisms.
Article
Biotechnology & Applied Microbiology
Na Zhang, Xin Guo, Tongzhu Han, Liju Tan, Qiaona Xue, Jiangtao Wang
Summary: In this study, allelochemicals were extracted and isolated from the filtrate of Phaeodactylum tricornutum, and it was found that these compounds had a significant inhibitory effect on the growth of Prorocentrum donghaiense. One specific compound, substance 6, was identified as having allelopathic activity, providing direct evidence of allelopathy between P. tricornutum and P. donghaiense.
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2022)
Article
Environmental Sciences
Jinju Ma, Chunyun Zhang, Fuguo Liu, Yin Liu, Yuanyuan Wang, Guofu Chen
Summary: A polymerase chain reaction-nucleic acid chromatography strip (PCR-NACS) method was established for the detection of Prorocentrum donghaiense. The PCR-NACS method is easy, sensitive, and visual, providing a potential new approach for the identification of P. donghaiense in natural water samples.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2023)
Article
Biochemical Research Methods
Xin Cao, Zhong Guo, Hualong Wang, Yuelei Dong, Songhui Lu, Qing-Yu He, Xuesong Sun, Gong Zhang
Summary: Harmful algal blooms are a global threat to marine ecosystems and human health. This study used translatome sequencing to investigate the molecular mechanisms of the Prorocentrum donghaiense algae. Through analyzing the translatome and proteome, the study found that up-regulation of energy and material production pathways in phosphor-rich conditions led to exponential growth of the algae in HABs. The researchers also demonstrated that mild translation delay using low concentrations of cycloheximide can control algal blooms without harming other aquatic organisms or humans.
JOURNAL OF PROTEOME RESEARCH
(2021)
Article
Engineering, Marine
Qianqian Wang, Xinguo Shi, Yisong Guo, Pin Lv, Yuying Zhong, Hui Xie, Jianfeng Chen
Summary: This study optimized the medium composition and culture conditions of a bacteria strain that can lyse Prorocentrum donghaiense, leading to a significant improvement in the algicidal activity. The findings provide insights for controlling algal blooms in the marine environment.
JOURNAL OF MARINE SCIENCE AND ENGINEERING
(2022)
Article
Biotechnology & Applied Microbiology
Xiao-Kun Hu, Zhuo-Ru Lin, Qing-Chun Zhang, Fan-Zhou Kong, Jing-Yi Cen, Yu-Lan Zeng, Ren-Cheng Yu
Summary: A real-time quantitative PCR assay was developed to improve the accuracy and efficiency in detection of Karenia mikimotoi. The qPCR assay, along with another assay for Prorocentrum donghaiense, was used to study a bloom of dinoflagellates in the coastal waters of Fujian province. The qPCR assays offer reliable and accurate approaches for the detection of harmful algal bloom species.
JOURNAL OF APPLIED PHYCOLOGY
(2022)
Article
Environmental Sciences
Liyan He, Zhiming Yu, Jianan Zhu, Xihua Cao, Xiuxian Song
Summary: This paper employed atomic force microscopy to study the harmful algal bloom species Prorocentrum donghaiense for the first time. The study revealed the typical structures on the cell surfaces and quantified the nanostructures and nanomechanical parameters. Under nitrogen limitation, the cell surface roughness and adhesion decreased, and the elastic modulus increased. These changes reflect the potential alterations in the physiological functions of P. donghaiense cells under external environmental changes.
FRONTIERS IN MARINE SCIENCE
(2022)
Article
Environmental Sciences
Jinju Ma, Fuguo Liu, Yin Liu, Chunyun Zhang, Yuanyuan Wang, Guofu Chen
Summary: This study proposed a detection technology for harmful microalgae using the nuclease protection assay integrated with dot nucleic acid chromatography strip. With specific probes and optimized parameters, this method enables rapid, simple, and visual detection of harmful microalgae.
MARINE POLLUTION BULLETIN
(2022)
Article
Food Science & Technology
Epaminondas D. Christou, Ioanna Varkitzi, Isabel Maneiro, Soultana Zervoudaki, Kalliopi Pagou
Summary: Researchers conducted a series of experiments to study the effects of toxic dinoflagellate on the feeding, reproduction, and toxin excretion of copepods. They found that the toxicity of the dinoflagellate affected the reproduction, toxin excretion, and feeding behavior of the copepods, indicating the significant impact of toxins on copepods.
Article
Environmental Sciences
Zhihao Zhang, Yunyun Zhuang, Hongju Chen, Songhui Lu, Yixuan Li, Ruping Ge, Chang Chen, Guangxing Liu
Summary: Blooms of the non-toxic dinoflagellate Prorocentrum donghaiense in the East China Sea significantly changed the structure and functions of the zooplankton community, leading to an increase in species richness and biodiversity after the bloom. During the bloom, the zooplankton community was dominated by carnivorousand egg-brooding- lineages represented by small jellyfish, chaetognaths, and copepods.
MARINE POLLUTION BULLETIN
(2021)
Article
Marine & Freshwater Biology
Jian-Wei Zheng, Guo-Hui He, Rui-Xia Xu, Xiang Wang, Hong-Ye Li, Wei-Dong Yang
Summary: Studies have found that there are reciprocal inhibitory effects between Karenia mikimotoi and Prorocentrum shikokuense under co-cultures. RNA sequencing analysis revealed that genes involved in photosynthesis, carbon fixation, energy metabolism, nutrients absorption and assimilation were up-regulated in K. mikimotoi after co-cultured with P. shikokuense, while genes involved in DNA replication and cell cycle were down-regulated. In contrast, genes involved in energy metabolism, cell cycle, and nutrients uptake and assimilation were down-regulated in P. shikokuense under co-culture with K. mikimotoi. Additionally, the expression of PLA2G12 and nitrate reductase, which may play important roles in the allelopathy of K. mikimotoi, was significantly increased. These findings provide new insights into the interspecies competition between K. mikimotoi and P. shikokuense and offer a novel strategy for studying interspecific competition in complex systems.
Article
Environmental Sciences
Jiazhu Zhang, Lingwei Kong, Yan Zhao, Qingming Lin, Shaojie Huang, Yafang Jin, Zengling Ma, Wanchun Guan
Summary: Bibliometric network analysis reveals that microplastics have detrimental effects on marine organisms. However, the combined effects of microplastics and climate change are not well understood. This study investigates the combined effects of microplastics and simulated ocean warming on a red tide species, Prorocentrum donghaiense. The results show that microplastics decrease cell growth, pigment contents, and electron transfer rate, but increase reactive oxygen species and antioxidant enzyme levels at low temperature. High temperatures have positive and negative effects depending on microplastic concentrations. These findings expand our knowledge on microalgae responses to increasing microplastic pollution in future warming scenarios.
ENVIRONMENTAL POLLUTION
(2022)
Article
Environmental Sciences
Xiansheng Zhang, Guangming Zhen, Xiaoru Cui, Yulan Zeng, Weimin Gao, Kunlong Yu, Keqiang Li
Summary: Blooms of Prorocentrum donghaiense occur annually in the East China Sea coastal waters, causing damage to the ecosystem and hindering economic development. Dissolved organic nitrogen and phosphorus are found to be the key factors influencing the bloom and phytoplankton community structure.
MARINE ENVIRONMENTAL RESEARCH
(2023)
Article
Marine & Freshwater Biology
Songhui Lu, Linjian Ou, Xinfeng Dai, Lei Cui, Yuelei Dong, Pengbin Wang, Dongmei Li, Douding Lu
Summary: Prorocentrum donghaiense is a bloom-forming dinoflagellate species that occurs annually in the East China Sea, with distinct succession patterns correlated with environmental factors. The blooms have impacts on marine ecosystems and further research is needed on nutrient budgets and socio-economic impacts.
Article
Dermatology
Jiaxing You, Chao Liu, Yixin Chen, Weifen Zhu, Hongye Li, Lin Li
Summary: The aim of this study was to identify the most cited articles in the field of diabetic foot ulcer (DFU) research. The study conducted a bibliometric analysis using Boolean search terms in two databases. The findings revealed the characteristics of the top-cited articles, such as publication year, country of origin, and level of evidence. The majority of the articles were clinical studies, providing valuable insights into the history and development of DFU research.
INTERNATIONAL JOURNAL OF LOWER EXTREMITY WOUNDS
(2023)
Article
Green & Sustainable Science & Technology
Xiang Wang, Si-Fen Liu, Zhen-Yao Wang, Yao Wang, Ting-Bin Hao, Srinivasan Balamurugan, Da-Wei Li, Yuhe He, Hong-Ye Li, Carol Sze Ki Lin
Summary: The research on sustainable alternatives for producing valuable polyunsaturated fatty acids (PUFAs) has been promoted due to the limited supply of wild deep-sea fish and the potential accumulation of toxic pollutants. In this study, an evolved strain of Phaeodactylum tricornutum microalgae was generated through adaptive evolution, resulting in enhanced biomass and PUFA production capabilities. The use of food waste hydrolysate as a carbon source further improved biomass yield and PUFA production. This study provides novel insights into sustainable microalgal-based waste valorisation for PUFA production.
JOURNAL OF CLEANER PRODUCTION
(2022)
Article
Microbiology
Yunpeng Gai, Lei Li, Bing Liu, Haijie Ma, Yanan Chen, Fang Zheng, Xuepeng Sun, Mingshuang Wang, Chen Jiao, Hongye Li
Summary: This study identifies the crucial role of bZIP transcription factors in fungal morphogenesis, abiotic stress response, and pathogenesis in phytopathogenic fungus Alternaria alternata. The findings expand our understanding of how microbial pathogens utilize these genes to cope with environmental stresses and achieve successful infection in the host plant.
MICROBIOLOGICAL RESEARCH
(2022)
Article
Microbiology
Pu-Dong Li, Zeng-Rong Zhu, Yunzeng Zhang, Jianping Xu, Hongkai Wang, Zhengyi Wang, Hongye Li
Summary: By studying the impact of Diaporthe citri on the phyllosphere microbiome of citrus, we found that infection caused changes in the phyllosphere microbiome. After infection, there was a significant decrease in community evenness, the emergence of new microbes, and an intense microbial network. We also identified potential beneficial microbes and enriched microbial functions that could help plants cope with D. citri challenge.
Article
Food Science & Technology
Mei-Hua Ye, Da-Wei Li, Qiu-Die Cai, Yu-Hu Jiao, Yang Liu, Hong-Ye Li, Wei-Dong Yang
Summary: In this study, it was found that different bivalves accumulated varying levels of DSP toxins and displayed different toxic responses after exposure to P. lima. The Nrf2 pathway may play a role in the bivalves' defense against DSP toxins.
Article
Engineering, Environmental
Yuhan Ma, Wasiqi Yuan, Wenjie Kan, Changyue Huang, Jiawei Zhu, Guilong Zhang, Hongye Li, Srinivasan Balamurugan, Lifang Wu
Summary: Microalgae are being investigated as green cell factories for biochemistry and pollution control. This study focuses on enhancing the synthesis of selenium nanoparticles (SeNPs) in microalgae and understanding the molecular mechanisms involved. Chlorella vulgaris was fortified with quercetin to promote SeNPs biotransformation. Quercetin facilitated the formation of SeNPs within 72 hours, converting almost 97% of inorganic selenite to SeNPs. Electron microscopy confirmed the biogenic ability of the algal system. Quercetin fortification protected C. vulgaris from selenite-induced impairment and improved its growth rate, while also altering the fatty acid profile.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Agronomy
Bei Liu, Xingxing Liang, Jinchao Kong, Chen Jiao, Hongye Li, Yunpeng Gai
Summary: In this study, the genetic structure and diversity of Colletotrichum gloeosporioides, the dominant species causing citrus anthracnose, were analyzed using the Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene. A total of 19 GAPDH haplotypes were identified, with the main haplotype (haplotype 5) distributed widely among isolates from different Citrus species and locations. The genetic differentiation index (Fst) revealed significant genetic differentiation between populations from different regions and host species. The results suggest that genetic differentiation in C. gloeosporioides is mainly influenced by gene mutation, recombination, or migration within native populations rather than natural selection caused by geography or host variety.
Article
Agriculture, Multidisciplinary
Li-Gong Zou, Yi-Tong Yao, Fu-Fang Wen, Xiao Zhang, Bo-Tao Liu, Da-Wei Li, Yu-Feng Yang, Wei-Dong Yang, Srinivasan Balamurugan, Hong-Ye Li
Summary: The excessive accumulation of catechol in the environment due to its burgeoning commercial applications poses a severe ecological threat. This study investigated the potential of the microalga Crypthecodinium cohnii to degrade catechol and utilize the byproduct as a carbon source. Results showed that catechol significantly increased the growth of C. cohnii and was rapidly catabolized within 60 hours. Transcriptomic analysis identified key genes involved in catechol degradation, and real-time polymerase chain reaction (RT-PCR) analysis revealed a remarkable increase in the transcription of these genes. The study also demonstrated altered primary metabolite content and the ability of C. cohnii to tolerate catechol treatment without adverse effects.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Green & Sustainable Science & Technology
Zi-Hao Qin, Xiaomeng Hu, Jin-Hua Mou, Guo-Hui He, Guang-Bin Ye, Hong-Ye Li, Shauhrat Singh Chopra, Liang Dong, Carol Sze Ki Lin, Xiang Wang
Summary: This study conducted Life Cycle Assessments (LCA) to evaluate the environmental impact of laboratory-scale EPA production by Phaeodactylum tricornutum. The results showed that technical advancements, particularly metabolic engineering, significantly reduced the global warming potential and cumulative energy demand associated with EPA production. Electricity consumption and extraction solvents were identified as the main contributors to the environmental impact. The study suggests future directions should focus on increasing EPA productivity, adopting alternative cultivation approaches with less energy input, and implementing environmentally friendly methods for EPA recovery.
JOURNAL OF CLEANER PRODUCTION
(2023)
Article
Chemistry, Medicinal
Kuan-Kuan Yuan, Zi-Min Chen, Ya-Xin Liu, Hong-Ye Li, Wei-Dong Yang
Summary: This study found that docosahexaenoic acid (DHA) can enhance the response of Perna viridis to diarrhetic shellfish toxins (DSTs) produced by Prorocentrum lima. The addition of DHA increased the esterification level of DSTs, activated the Nrf2 signaling pathway, and alleviated the damage to the digestive glands. These findings provide insights into the role of DHA in the environmental adaptation of bivalves.
Article
Agronomy
Yunpeng Gai, Qichen Niu, Jinchao Kong, Lei Li, Xingxing Liang, Yuwei Cao, Xianqi Zhou, Xuepeng Sun, Haijie Ma, Mingshuang Wang, Neeraj Shrivastava, Hongye Li, Chen Jiao
Summary: A comparative transcriptome analysis was performed to study the gene expression patterns of Alternaria alternata during infection of citrus. Significant gene expression differences were found between the early and late stages of infection. Several novel genes related to plant-pathogen interaction were identified. RT-qPCR analysis confirmed the expression patterns of selected genes from the peroxisome pathway.
Article
Microbiology
Wen Wang, Tao Xiong, Yating Zeng, Wenwen Li, Chen Jiao, Jianping Xu, Hongye Li
Summary: This study conducted an extensive survey of Phyllosticta species across 11 citrus-producing provinces in southern China and identified five Phyllosticta species with pathogenicity on different citrus species. The findings have important implications for the control and management of Citrus Black Spot and related diseases.
Article
Engineering, Environmental
Jin-Hua Mou, Zi-Hao Qin, Yu-Feng Yang, Si-Fen Liu, Wei Yan, Lan Zheng, Ya-Hui Miao, Hong-Ye Li, Patrick Fickers, Carol Sze Ki Lin, Xiang Wang
Summary: This study investigated the characteristics of four types of food waste and their hydrolysates, and explored their potential application in biorefineries. The results showed that the characteristics of food waste and their hydrolysates varied based on their origins, and the origin and storage conditions of food waste affected microalgal growth and paramylon production.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Marine & Freshwater Biology
Jian-Wei Zheng, Guo-Hui He, Rui-Xia Xu, Xiang Wang, Hong-Ye Li, Wei-Dong Yang
Summary: Studies have found that there are reciprocal inhibitory effects between Karenia mikimotoi and Prorocentrum shikokuense under co-cultures. RNA sequencing analysis revealed that genes involved in photosynthesis, carbon fixation, energy metabolism, nutrients absorption and assimilation were up-regulated in K. mikimotoi after co-cultured with P. shikokuense, while genes involved in DNA replication and cell cycle were down-regulated. In contrast, genes involved in energy metabolism, cell cycle, and nutrients uptake and assimilation were down-regulated in P. shikokuense under co-culture with K. mikimotoi. Additionally, the expression of PLA2G12 and nitrate reductase, which may play important roles in the allelopathy of K. mikimotoi, was significantly increased. These findings provide new insights into the interspecies competition between K. mikimotoi and P. shikokuense and offer a novel strategy for studying interspecific competition in complex systems.
Article
Public, Environmental & Occupational Health
Jinping Zhang, Peng Liu, Morong Wang, Jie Wang, Jie Chen, Wenling Yuan, Mei Li, Zhijuan Xie, Wangping Dong, Hongye Li, Yan Zhao, Lun Wan, Tian Chu, Lu Wang, Hui Zhang, Ting Tao, Jing Ma
Summary: This study analyzed the clinical features and diagnostic methods of 19 patients with COVID-19. The findings showed that there were more male than female critically ill patients, and they often had symptoms such as fever and dry cough. Chest computed tomography revealed ground-glass changes in all cases. Significant changes were observed in clinical laboratory indices, especially in the D-dimer level of non-survivors.
JOURNAL OF PUBLIC HEALTH-HEIDELBERG
(2022)