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Effects of Metformin on the motility and acrosome reaction of fresh and frozen-thawed Chicken spermatozoa

PUBLISHED June 10, 2024 (DOI: https://doi.org/10.54985/peeref.2406p5604438)



Thi Mong Diep Nguyen1 , François Seigneurin2 , Elisabeth Blesbois2
  1. Quy Nhon University, Binh Dinh, Vietnam
  2. INRAe, Nouzilly, France

Conference / event

11th Days of Poultry Research and Foie Gras Palmipeds, March 2015 (Tours, France)

Poster summary

AMP-activated protein kinase (AMPK) is a key regulator of energy metabolism. Metformin is an activator of AMPK. Recently, we have characterized AMPK in spermatozoa and found it to be involved in fundamental functions: motility and the ability to carry out the acrosome reaction. However, AMPK role in frozen-thawed spermatozoa is still unknown. Spermatozoa are highly specialized cells that require a great deal of energy to ensure their motility and fertilization capacity. The aims of this study were to investigate the effects and molecular mechanism of metformin in frozen-thawed spermatozoa. Our results showed that chicken spermatozoa cryopreserved with metformin had improved membrane integrity, motility, and acrosome response. This is the first assessment of the effects of metformin on frozen-thawed chicken spermatozoa through its influence on AMPK activity to reduce damage to avian semen caused by cryopreservation. These data will be useful for developing and improving avian semen handling and storage techniques.


AMP-activated protein kinase, AMPK activator, Avian semen, Chicken spermatozoa, Frozen-thawed spermatozoa, Metformin

Research areas

Veterinary and Animal Sciences, Biological Sciences


  1. TMD Nguyen, Alves S, Grasseau I, Métayer-Coustard S, Praud C, Froment P, Blesbois E. Central Role of 5'-AMP-Activated Protein Kinase in Chicken Sperm Functions. Biology of reproduction 91: 121, 1-15
  2. TMD Nguyen, Seigneurin F, Froment P, Combarnous Y, Blesbois E. The 5’-AMP-activated protein kinase (AMPK) is involved in the antioxidant defenses and cryopreserved chicken sperm functions. PLoS One 10(7): e0134420
  3. TMD Nguyen, Y Combarnous, C Praud, A Duittoz, E Blesbois. Ca2+/calmodulin-dependent protein kinase kinase (CaMKK) effect on AMP-activated Protein Kinase (AMPK) regulation of chicken sperm functions. PLoS One 11(1):e0147559.
  4. TMD Nguyen, P Froment, Y Combarnous, E Blesbois. Rôle de l’AMPK des spermatozoïdes dans le contrôle de la reproduction. Medecine Sciences n° 5, vol. 32 : 491-496.
  5. TMD Nguyen, Y Combarnous, A Duittoz, E Blesbois. Calcium channels in the regulation of chicken sperm motility and acrosome reaction capacity. The FEBS journal 283 (10), 1902-1920.


  1. French Agence Nationale de la Recherche
  2. French National Science Infrastructure CRB-Anim

Supplemental files

No data provided

Additional information

Competing interests
No competing interests were disclosed.
Data availability statement
The datasets generated during and / or analyzed during the current study are available from the corresponding author on reasonable request.
Creative Commons license
Copyright © 2024 Nguyen et al. This is an open access work distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Nguyen, T., Seigneurin, F., Blesbois, E. Effects of Metformin on the motility and acrosome reaction of fresh and frozen-thawed Chicken spermatozoa [not peer reviewed]. Peeref 2024 (poster).
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