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BNN27 nanoformulations for nose-to-brain delivery

PUBLISHED June 21, 2023 (DOI: https://doi.org/10.54985/peeref.2306p3373108)



Maria Kannavou1 , Kanelina Karali2 , Theodora Katsila3 , Eleni Siapi3 , Antonia Marazioti1 , Pavlos Klepetsanis1 , Theodora Calogeropoulou3 , Ioannis Charalampopoulos2 , Sophia G. Antimisiaris1
  1. University of Patras and FORTH/ICE-HT
  2. University of Crete and IMBB/FORTH
  3. Institute of Chemical Biology, National Hellenic Research Foundation

Conference / event

6th Nanomed Workshop, July 2023 (Patras, Greece)

Poster summary

Intranasal administration offers an alternative way for brain targeting. In this project, we studied the design of two innovative mucoadhesive nanoformulations for BNN27 intranasal delivery. Negatively-charged and uncharged liposomes were coated with chitosan (CHT) at different CHT/lipid ratios. Medium MW CHT-coated PC/PG/BNN27 liposomes at 0.1w/w CHT/lipid ratio demonstrated highest coating efficiency & mucoadhesive properties. Furthermore, preparation of mucoadhesive nanoemulsions (MNEs) was studied. NEs were prepared by the spontaneous emulsification method using Capmul MCM as oily phase. MNEs with 10%w/w Capmul MCM and 0.3%w/w CHT, displayed optimal physicochemical characteristics. Optimal nanoformulations were evaluated for their permeability across a cellular BBB model (hCMEC/D3 cell monolayers) in vitro. Also, their in vivo fate after intranasal administration revealed the superiority of CHT-NEs for more efficient BNN27 distribution to the brain, compared to CHT-LIPs. Current results prove that CHT-NEs succeed to deliver more BNN27 to the brain (and faster), compared to CHT-coated LIPs.


BNN27, Mucoadhesive nanoformulations, Intranasal delivery

Research areas

Pharmacy and Pharmacology


  1. Gravanis, A.; Pediaditakis, I.; Charalampopoulos, I. Synthetic Microneurotrophins in Therapeutics of Neurodegeneration. Oncotarget 2017, 8, 9005–9006
  2. Kannavou, M.; Karali, K.; Katsila, T.; Siapi, E.; Marazioti, A.; Klepetsanis, P.; Calogeropoulou, T.; Charalampopoulos, I.; Antimisiaris, S.G. Development and Comparative In Vitro and In Vivo Study of BNN27 Mucoadhesive Liposomes and Nanoemulsions for Nose-to-Brain Delivery. Pharmaceutics 2023, 15, 419
  3. Markoutsa, E.; Papadia, K.; Giannou, A.D.; Spella, M.; Cagnotto, A.; Salmona, M.; Stathopoulos, G.T.; Antimisiaris, S.G. Mono and Dually Decorated NanoLIPs for Brain Targeting, in vitro and in vivo Studies. Pharm. Res. 2014, 31, 1275–1289.


  1. European Regional Development Fund of the European Union and Greek national funds

Supplemental files

No data provided

Additional information

Competing interests
No competing interests were disclosed.
Data availability statement
The datasets generated during and / or analyzed during the current study are available from the corresponding author on reasonable request.
Creative Commons license
Copyright © 2023 Kannavou et al. This is an open access work distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Kannavou, M., Karali, K., Katsila, T., Siapi, E., Marazioti, A., Klepetsanis, P., Calogeropoulou, T., Charalampopoulos, I., Antimisiaris, S. BNN27 nanoformulations for nose-to-brain delivery [not peer reviewed]. Peeref 2023 (poster).
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