Evaluation of cryopreservation solutions based on human platelet lysate for bioengineered bissues aimed for advanced therapy treatments

Authors

María Martín-López¹ , Cristina Rosell-Valle¹ , Blanca Arribas-Arribas¹ , Rafael Campos-Cuerva¹ , Inma Piudo¹ , Isidora Ranchal¹ , Beatriz Fernandez-Muñoz¹ , Miguel Alaminos² , Gloria Carmona-Sánchez¹ , Mónica Santos-González³

1. Red Andaluza de diseño y traslación de Terapias Avanzadas, (RAdytTA), Sevilla, Spain
2. University of Granada, Spain
3. Fundación Pública Andaluza para la Gestión de la Investigación en Salud yen Sevilla (FISEVI), Sevill

Conference / event

ISCT 2021 New Orleans Virtual Annual Meeting, May 2021 (Virtual)

Poster summary

There remains much interest in improving cryopreservation techniques for advanced therapy medicinal products. Here, we evaluated several xenogeneic-free, pathogen-inactivated and non-inactivated human platelet lysate-based formulations for the cryopreservation of bioengineered tissues (artificial dermis). The formulations are under a bidding process with MD Bioproducts (Zurich, Switzerland) and are proprietary. Amongst all formulated solutions, solution 5' preserved the viability and the phenotype of embedded cells and preserved the matrix integrity and biomechanical properties similar to those of the standard cryopreservation solution. The solutions were stable at -20ºC for 3 and 18 months, respectively. Finally, we established that there is, at least a 2-hour window in which thawed tissue and cells, maintain optimum viability immersed in the formulated solutions. In summary, our results show that inactivated human platelet lysate-based solutions are efficient and safe candidates to cryopreserve bioengineered tissues and offer an alternative to commercially available cryoprotective solutions.

Keywords

Cryopreservation, Bioengineered tissue, Freezing, HPL, ATMP

Research areas

Bioengineering, Biological Sciences, Molecular and Cellular Biosciences

References

1. Serrato, D. et al. Comparison of the effect of cryopreservation protocols on the histology of bioengineered tissues. Histol. Histopathol. 24, 1531–1540 (2009).
2. Rodriguez, M. A. et al. Cryopreservation of an artificial human oral mucosa stroma. A viability and rheological study. Cryobiology 67, 355–362 (2013).

Funding

1. FEDER/Ministerio de Ciencia, Innovación y Universidades-Agencia Estatal de Investigación (No. TC-2017-6658-1-Proyecto)

Supplemental files

1. Research article published in Stem Cell Research & Therapy   Download

Additional information

Competing interests

MM-L, CR-V and MS-G present competing interests. The commercial exploitation rights of the cryoprotective solutions based on human platelet lysate for cryopreservation of cells and tissue referred to in the present publication were licensed in exclusive, worldwide, for research use and with the right to sublicense to the company MD Bioproducts GmbH.

Data availability statement

The datasets generated during and / or analyzed during the current study are available from the corresponding author on reasonable request.

Creative Commons license

Copyright © 2023 Martín-López et al. This is an open access work distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.