Identification of Novel Lectins from Freshwater Prawn, Macrobrachium rosenbergii, and Expression Analysis in Response to Vibrio harveyi and M. rosenbergii nodavirus

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Authors

Snigdha Baliarsingh¹ , Bharat Bhusan Patnaik¹

1. Fakir Mohan University

Conference / event

FMU Research Scholar Conclave, October 2021 (Balasore, Odisha, India)

Poster summary

Lectins are PRRs that binds to PAMPs and participate in the innate immunity of the host. We identified and characterized novel lectins from the freshwater prawn, Macrobrachium rosenbergii such as MrCTL and Mr(T)Lec-4 showing C-type lectin domain and MrERGIC-53 showing L-type lectin domain. MrCTL, Mr(T)Lec-4, and MrERGIC-53 encoded polypeptides of 217, 330, and 504 amino acids, respectively. While Mr(T)Lec-4 mRNA upregulated 6h in the hepatopancreas post-infection with Vibrio harveyi, MrCTL and MrERGIC-53 mRNA showed upregulation in stomach and intestine following M. rosenbergii nodavirus (MrNV) challenge. This demonstrates that the novel lectins putatively participate in the innate immunity of the host.

Keywords

Macrobrachium rosenbergii, Lectins, Inate immunity, Vibrio harveyi, M. rosenbergii nodavirus, Expression analysis

Research areas

Bioinformatics and Genomics, Genetics, Zoology, Biological Sciences, Molecular and Cellular Biosciences

References

1. Baliarsingh, S., Dash, M., Dewangan, N., Hameed, A.S.S., Mohanty, J., Lee, Y.S., Patnaik, B.B. (2019). Upregulation of hepatopancreas associated lectin transcripts in Macrobrachium rosenbergii in response to Vibrio harveyi and M. rosenbergii nodavirus. Journal of Environmental Biology 40(5), 969-976.
2. Baliarsingh, S., Sahoo, S., Jo, Y.H., Han, Y.S., Sarkar, A., Lee, Y.S., Mohanty, J., Patnaik, B.B. (2022). Molecular cloning, sequence characterization, and expression analysis of C-type lectin (CTL) and ER-Golgi intermediate compartment 53-kDa protein (ERGIC-53) homologs from the freshwater prawn, Macrobrachium rosenbergii. Aquaculture International 30, 1011-1035.

Funding

1. Department of Biotechnology, Government of India (No. BT/PR12710/AAQ/03/713/2015)

Supplemental files

1. Photograph of poster presentation at FMU Research Scholar Conclave.   Download

Additional information

Competing interests

None declared.

Data availability statement

The datasets generated during and / or analyzed during the current study are available from the corresponding author on reasonable request.

Creative Commons license

Copyright © 2022 Baliarsingh et al. This is an open access work distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.