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Optimizing Three-Dimensional Bioprinting for Cell Culture Scaffolds

PUBLISHED July 21, 2022 (DOI: https://doi.org/10.54985/peeref.2207p5266153)



Emma Brudos1 , Miranda Nelson2 , David Estrada2
  1. New Mexico Institute of Mining and Technology
  2. Boise State University

Conference / event

Idaho Conference of Undergraduate Research, July 2022 (Virtual)

Poster summary

In order to increase the success rates of leukemia treatments, a greater understanding of cell interactions within the bone marrow is required. 3D bioprinting offers a solution for studying the cells in vitro by printing scaffolds that mimic the internal bone structure. Our research focused on evaluating a Cellink BioX printer in order to print custom scaffolds for 3D cell culture. These can be utilized in an environment that mimics the physiological conditions of trabecular bone to study treatments. The print conditions were optimized to produce scaffolds that had porosity and structure within the range of real human trabecular bone.


Bioprinting, Trabecular bone, Tissue engineering, Bone marrow, 3D cell culture

Research areas

Material Sciences, Bioengineering


  1. Barrett AJ, Battiwalla M. Relapse after allogeneic stem cell transplantation. Expert Rev Hematol. 2010 Aug;3(4):429-41. doi: 10.1586/ehm.10.32. PMID: 21083034; PMCID: PMC3426446.
  2. National Cancer Institute. Definition of bone marrow. Retrieved from https://www.cancer.gov/publications/dictionaries/cancer-terms/def/bone-marrow
  3. A cross section of a human long bone SVG file by Pbroks13, distributed under a Creative Commons Attribution 3.0 Unported license. Retrieved from https://commons.wikimedia.org/w/index.php?curid=5188772
  4. O'Connell, C., et al. (2021). Characterizing Bioinks for Extrusion Bioprinting: Printability and Rheology. Methods in molecular biology. 2140:111-133 https://www.researchgate.net/publication/340110752_Characterizing_Bioinks_for_Extrusion_Bioprinting_Printability_and_Rheology
  5. Lee, S., Porter, M., Wasko, S. et al. Potential Bone Replacement Materials Prepared by Two Methods. MRS Online Proceedings Library 1418, 177–188 (2012). https://doi.org/10.1557/opl.2012.671


  1. National Science Foundation (No. XC-1950305 and NSF-DMR 1710640)
  2. National Science Foundation Career Award (No. 1848516)

Supplemental files

No data provided

Additional information

Competing interests
None declared.
Data availability statement
Data sharing not applicable to this poster as no datasets were generated or analyzed during the current study.
Creative Commons license
Copyright © 2022 Brudos et al. This is an open access work distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Brudos, E., Nelson, M., Estrada, D. Optimizing Three-Dimensional Bioprinting for Cell Culture Scaffolds [not peer reviewed]. Peeref 2022 (poster).
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